LOCUS       MR218567                9327 bp    DNA     circular  SYN 
DEFINITION  Synj2 (Myc-DDK-tagged) - Mouse synaptojanin 2 (Synj2), transcript variant 1
ACCESSION   NM_001113353
VERSION     NM_001113353.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5482..5573 
                     /label="Myc-DDK"  
     misc_feature    5603..6188 
                     /label="PolyA signal"  
     misc_feature    5660..5679 
                     /label="Restriction Enzyme"  
     misc_feature    6464..7136 
                     /label="ColE1"  
     misc_feature    7566..8360 
                     /label="Kan/Neo"  
     misc_feature    8395..8697 
                     /label="SV40 ori"  
     misc_feature    8783..9260 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5457..5464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5457 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTAAGC AAAGGGCTGC GGCTGCTGGC GCGGCTGGAC CCCACTGGCC 
     1081 CGAGCAGCGT GCTGTTGGAG GCGCGGGGCC GCGGCGACTG CCTGCTCTTC GAGGCCGGCG 
     1141 CAGTGGCCAC GCTGGCTCCG GAAGAGAAGG AAGTCATTAA AGGACTGTAT AGCAAGCTGA 
     1201 CGGATGCCTA TGGCTGCCTC GGGGAGCTGA GGTTACAATC CGGTGGCGTC CCCTTGAGCT 
     1261 TTCTGGTGTT GGTGACAGGC TGCATGTCAG TGGGCAGAAT TCCAGATGCA GAGATCTACA 
     1321 AAATCACTGC CACTGAGTTG TACCCCCTGC AGGAAGAGGC CAAGGAAGAG GACCGCCTGC 
     1381 CCACCTTAAA GAAAATCCTG AGCTCAGGGG TGTTCTATTT CGCATGGCCC AATGATGGCG 
     1441 CCTGCTTCGA TCTGACCATC AGGGCTCAGA AACAGGGTGA TGACGGCTCT GAATGGGGGA 
     1501 CCTCTTTCTT CTGGAACCAG CTATTGCATG TGCCTCTGCG GCAGCACCAG GTGAACTGTC 
     1561 ATAACTGGTT GCTGAAAGTC ATCTGTGGGG TGGTGACCAT CCGCACAGTA TATGCCTCCC 
     1621 ACAAGCAGGC CAAGGCCTGT CTCATCTCTC GCATCAGCTG TGAACGCGCA GGTGCTCGCT 
     1681 TCCTCACCCG TGGTGTGAAC GATGATGGCC ACGTGTCCAA CTTTGTGGAG ACAGAGCAGA 
     1741 CGATTTACAT GGATGATGGA GTATCGTCCT TTGTCCAGAT CCGAGGCTCC GTTCCGCTGT 
     1801 TCTGGGAGCA ACCAGGACTT CAGGTTGGCT CCCATCATCT GAGACTGCAC AGAGGCCTAG 
     1861 AGGCCAACGC TCCTGCTTTT GAAAGGCACA TGGTGCTTCT GAAGGAGCAA TACGGTAAGC 
     1921 AGGTGGTTGT GAACCTGCTG GGTAGCAGAG GCGGTGAAGA GGTGCTCAAC AGAGCCTTCA 
     1981 AGAAGTTGCT CTGGGCTTCT TGCCACGCGG GTGACACACC TATGATAAAT TTTGACTTCC 
     2041 ATCAGTTTGC CAAAGGTAGG AAGCTAGAGA AATTGGAGAA CCTGTTGAGA CCTCAGTTAC 
     2101 AGCTACACTG GGAAGACTTC GGCGTGTTTG CGAAGGGCGA GAATGTAAGT CCACGGTTTC 
     2161 AGAAAGGCAC TCTGCGGATG AACTGTCTCG ACTGTCTGGA TAGAACCAAC ACTGTGCAGT 
     2221 GCTTCATTGC TCTTGAGGTC CTTCATCTGC AGCTTGAGAG CTTGGGGCTA AATTCAAAGC 
     2281 CCATCATTGA CCGTTTTGTG GAGTCCTTCA AAGCCATGTG GTCTCTGAAT GGGCACAGCC 
     2341 TGAGCAAGGT GTTCACAGGG AGCAGGGCCC TGGAAGGAAA GGCCAAGGTG GGAAAGCTGA 
     2401 AGGATGGGGC CCGATCCATG TCTCGCACCA TCCAGTCCAA CTTCTTCGAC GGGGTGAAGC 
     2461 AGGAGGCCAT CAAGCTACTG CTAGTCGGAG ATGTCTACAA TGAAGAGTCT ACAGACAAAG 
     2521 GACGGATGCT GCTGGACAAC ACGGCCCTTC TGGCGACCCC CAGGATCTTG AAGGCCATGA 
     2581 CAGAACGCCA GTCGGAATTC ACGAATTTCA AGCGCATCCA GATTGCTGTG GGGACCTGGA 
     2641 ATGTGAACGG AGGAAAGCAA TTCCGTAGCA ATCTCCTGGG GACGGCTGAG CTCACGGACT 
     2701 GGCTCCTAGA TGCTCCTCAG CTGTCAGGAG CAGTGGACTC CCAGGATGAT GGCAGTCCTG 
     2761 CTGACGTATT TGCCATCGGG TTTGAGGAGA TGGTGGAACT GAGTGCGGGA AATATTGTCA 
     2821 ATGCCAGCAC CACCAACAGG AAGATGTGGG GCGAGCAGCT TCAGAAAGCC ATCTCCCGCT 
     2881 CCCATCGGTA CATCCTCTTG ACCTCCGCAC AGCTGGTGGG CGTCTGTCTT TACATCTTTG 
     2941 TACGTCCGTA CCACGTCCCG TTCATCAGAG ACGTGGCCAT CGACACCGTG AAGACCGGCA 
     3001 TGGGGGGAAA GGCGGGGAAT AAGGGTGCTG TGGGCATCCG CTTCCAGCTC CACAGCACCA 
     3061 GTTTCTGCTT CGTCTGTAGC CACCTGACGG CTGGGCAGTC TCAGGTGAAG GAGAGGAATG 
     3121 AAGACTACCG GGAGATCACG CACAAACTCT CCTTCCCTTC GGGGAGAAAC ATATTTTCAC 
     3181 ATGATTACGT GTTTTGGTGT GGCGATTTCA ACTACCGTAT TGATCTTACT TACGAAGAAG 
     3241 TCTTCTATTT TGTTAAACGC CAAGACTGGA AGAAACTTAT GGAATTCGAT CAGTTACAGT 
     3301 TACAGAAATC AAGTGGAAAA ATTTTTAAAG ACTTTCATGA AGGAGCCGTT AACTTCGGAC 
     3361 CCACCTACAA GTATGACGTT GGATCAGCTG CCTACGACAC AAGTGACAAG TGCCGTACCC 
     3421 CAGCCTGGAC AGACAGGGTG CTGTGGTGGA GGAAGAAGCA TCCATATGAT AAAACAGCTG 
     3481 GTGAACTCAA CCTTCTAGAC AGCGATCTAG ACGGCGATCC CCAAATCAGA CACACCTGGT 
     3541 CTCCAGGCAC TCTGAAATAC TACGGCCGTG CAGAGCTGCA GGCGTCTGAT CACAGACCTG 
     3601 TGCTGGCCAT TGTGGAGGTG GAGGTTCAAG AGGTGGATGT AGGAGCCCGG GAGAGGGTCT 
     3661 TCCAGGAAGT GTCCTCTGTC CAAGGCCCGC TGGATGCCAC CGTTGTTGTA AACCTCCAGT 
     3721 CTCCAACCCT AGAAGAGAAA AATGAATTTC CAGAGGACCT GCGCACAGAA CTTATGCAGA 
     3781 CTTTGGGGAA TTATGGGACG ATTATTCTAG TCAGGATCAA CCAAGGGCAG ATGCTGGTGA 
     3841 CGTTTGCAGA CAGCCACTCG GCTCTCAGTG TGCTGGATGT GGATGGTATG AAGGTGAAAG 
     3901 GCAGGGCCGT GAAGATTCGA CCAAAGACCA AAGATTGGCT AGAAGGCCTG AGAGAGGAGC 
     3961 TTCTCCGGAA GCGAGACAGC ATGGCCCCTG TGTCTCCCAC CGCCAACTCC TGCTTGTTGG 
     4021 AGGAGAACTT TGACTTCTCG AGTCTGGACT ATGAGTCCGA AGGGGATGTT CTTGAAGAGG 
     4081 ATGAAGACTA TTTAGTGGAT GGGTTTGGCC AGCCTGTAGT CTCAGACAGT GAGCTCGGTG 
     4141 GAGACAACTC TTCCGACACC ATGAGCTCCT TGACACCCGC CAGCAAGTCT CCCGCCCTGG 
     4201 CTAAAAAGAA GCAACATCCA ACATACAAAG ACGACGCTCA CCTGGTGACC TTAAAGCAGG 
     4261 AGCTGGAAGT TGCTGGGAAT TTTCGCCACC GTTCTCCGAG CAGGTCCCTG TCCGTCCCCA 
     4321 ATAGGCCTCG GCCACCTCAC CCACCACAGA GACCCCCCCC TCCAACTGGC TTAATGGTGA 
     4381 AGAAGTCAGC CTCAGACGCG TCCATCTCTT CTGGCACTCA TGGACAATAT TCCATCTTGC 
     4441 AGACAGCGAA ACTTCTCCCA GGAGCACCCC AGCAACCACC CAAGGCTAGA ACTGGAATAA 
     4501 GTAAACCTTA CAACGTCAAA CAGATCAAAA CCACCAACGC TCAGGAGGCA GAAGCAGCTA 
     4561 TCCGGTGTCT CCTGGAAGCT AGCGGAGGGG TCCCGGAATC AGCCCCAGGT GCCATACCCC 
     4621 TGAGAAACCA AGGGTCTTCT AAGCCAGAGG CCACCCTGGG GCCCCCAGCC CTGCCCCGCC 
     4681 GGCCTGCTCC AAGGGTTCCC ACTATGAAGA AACCAACTTT GAGGAGGACA GGAAAGCCCA 
     4741 TGTTACCAGA AGAAAACTTT GAGCCACAGC CTGTCCATTT TACAATGGCT TCCCAGGAAA 
     4801 TGAACCTTGA GACCCCTCCT CCAATAACAG CTACTCCCAT CCCGCCTGTT CCCAAACCAA 
     4861 GAACACTTCA GCCTGGGAAA GGTGTTGAGG GGAGGCCAAG CAGCGGTAAG CCAGAACCAG 
     4921 ATGAAGCTCC TTCCGTGACA GGCACTGTGG AGTCACCACC TCCAGAGGCC CAGGAGGCCC 
     4981 CGTCTCTGGC TCCCAAGGTG CCTCCGAGGA GGAAGAAGTC TGCACCAGCG GCCTTCCACC 
     5041 TGCAGGTCCT GCAGAACAAC AGCCAAGTTC TCCAGGGCCT CACGTGCTCC AGCAGTTCTC 
     5101 CACCCTCACT GAAGCCCGAC ACCCACCCGC TCTGTCTACA AGTGGCACTT GGCACTTCAT 
     5161 CTGCCAGAAG CCCTGAAACC CATGGCCCCA GAGTGACAGA GCCCGAGGCG GCCTCTTTTC 
     5221 ATGGCAACTA TCCAGATCCC TTCTGGAGCC TTCTCCACCA CCCTAAGCTG TTGAACAATA 
     5281 CCTGGCTGTC CAAGAGCTCC GAGCCTTTAG ACGTGGGGTC CAGGAACCCT GAGAGGACAC 
     5341 ACACAGAGCC GGCACAGGTC AATGCATCGT TGGCTGAGAG GGGGCTTCCA CCAGACCATG 
     5401 GGGGAAAAGA CTTAAGTCAC TGGGTGACAG CTAGTAACAA AGACAAGAGG ACAACATTAG 
     5461 GTGTTAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5521 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5581 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5641 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5701 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5761 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5821 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5881 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5941 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     6001 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6061 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6121 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6181 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6241 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6301 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6361 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6421 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6481 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6541 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6601 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6661 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6721 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6781 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6841 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6901 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6961 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     7021 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7081 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7141 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7201 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7261 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7321 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7381 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7441 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7501 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7561 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7621 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7681 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7741 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7801 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7861 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7921 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7981 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     8041 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8101 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8161 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8221 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8281 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8341 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8401 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8461 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8521 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8581 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8641 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8701 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8761 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8821 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8881 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8941 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     9001 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9061 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9121 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9181 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9241 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9301 ATTTAACAAA AATTTAACGC GAATTTT
//