LOCUS       MR218552                8538 bp    DNA     circular  SYN 
DEFINITION  Synj2 (Myc-DDK-tagged) - Mouse synaptojanin 2 (Synj2), transcript variant 3
ACCESSION   NM_011523
VERSION     NM_011523.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4693..4784 
                     /label="Myc-DDK"  
     misc_feature    4814..5399 
                     /label="PolyA signal"  
     misc_feature    4871..4890 
                     /label="Restriction Enzyme"  
     misc_feature    5675..6347 
                     /label="ColE1"  
     misc_feature    6777..7571 
                     /label="Kan/Neo"  
     misc_feature    7606..7908 
                     /label="SV40 ori"  
     misc_feature    7994..8471 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4675 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGTGGGC AGAATTCCAG ATGCAGAGAT CTACAAAATC ACTGCCACTG 
     1081 AGTTGTACCC CCTGCAGGAA GAGGCCAAGG AAGAGGACCG CCTGCCCACC TTAAAGAAAA 
     1141 TCCTGAGCTC AGGGGTGTTC TATTTCGCAT GGCCCAATGA TGGCGCCTGC TTCGATCTGA 
     1201 CCATCAGGGC TCAGAAACAG GGTGATGACG GCTCTGAATG GGGGACCTCT TTCTTCTGGA 
     1261 ACCAGCTATT GCATGTGCCT CTGCGGCAGC ACCAGGTGAA CTGTCATAAC TGGTTGCTGA 
     1321 AAGTCATCTG TGGGGTGGTG ACCATCCGCA CAGTATATGC CTCCCACAAG CAGGCCAAGG 
     1381 CCTGTCTCAT CTCTCGCATC AGCTGTGAAC GCGCAGGTGC TCGCTTCCTC ACCCGTGGTG 
     1441 TGAACGATGA TGGCCACGTG TCCAACTTTG TGGAGACAGA GCAGACGATT TACATGGATG 
     1501 ATGGAGTATC GTCCTTTGTC CAGATCCGAG GCTCCGTTCC GCTGTTCTGG GAGCAACCAG 
     1561 GACTTCAGGT TGGCTCCCAT CATCTGAGAC TGCACAGAGG CCTAGAGGCC AACGCTCCTG 
     1621 CTTTTGAAAG GCACATGGTG CTTCTGAAGG AGCAATACGG TAAGCAGGTG GTTGTGAACC 
     1681 TGCTGGGTAG CAGAGGCGGT GAAGAGGTGC TCAACAGAGC CTTCAAGAAG TTGCTCTGGG 
     1741 CTTCTTGCCA CGCGGGTGAC ACACCTATGA TAAATTTTGA CTTCCATCAG TTTGCCAAAG 
     1801 GTAGGAAGCT AGAGAAATTG GAGAACCTGT TGAGACCTCA GTTACAGCTA CACTGGGAAG 
     1861 ACTTCGGCGT GTTTGCGAAG GGCGAGAATG TAAGTCCACG GTTTCAGAAA GGCACTCTGC 
     1921 GGATGAACTG TCTCGACTGT CTGGATAGAA CCAACACTGT GCAGTGCTTC ATTGCTCTTG 
     1981 AGGTCCTTCA TCTGCAGCTT GAGAGCTTGG GGCTAAATTC AAAGCCCATC ATTGACCGTT 
     2041 TTGTGGAGTC CTTCAAAGCC ATGTGGTCTC TGAATGGGCA CAGCCTGAGC AAGGTGTTCA 
     2101 CAGGGAGCAG GGCCCTGGAA GGAAAGGCCA AGGTGGGAAA GCTGAAGGAT GGGGCCCGAT 
     2161 CCATGTCTCG CACCATCCAG TCCAACTTCT TCGACGGGGT GAAGCAGGAG GCCATCAAGC 
     2221 TACTGCTAGT CGGAGATGTC TACAATGAAG AGTCTACAGA CAAAGGACGG ATGCTGCTGG 
     2281 ACAACACGGC CCTTCTGGCG ACCCCCAGGA TCTTGAAGGC CATGACAGAA CGCCAGTCGG 
     2341 AATTCACGAA TTTCAAGCGC ATCCAGATTG CTGTGGGGAC CTGGAATGTG AACGGAGGAA 
     2401 AGCAATTCCG TAGCAATCTC CTGGGGACGG CTGAGCTCAC GGACTGGCTC CTAGATGCTC 
     2461 CTCAGCTGTC AGGAGCAGTG GACTCCCAGG ATGATGGCAG TCCTGCTGAC GTATTTGCCA 
     2521 TCGGGTTTGA GGAGATGGTG GAACTGAGTG CGGGAAATAT TGTCAATGCC AGCACCACCA 
     2581 ACAGGAAGAT GTGGGGCGAG CAGCTTCAGA AAGCCATCTC CCGCTCCCAT CGGTACATCC 
     2641 TCTTGACCTC CGCACAGCTG GTGGGCGTCT GTCTTTACAT CTTTGTACGT CCGTACCACG 
     2701 TCCCGTTCAT CAGAGACGTG GCCATCGACA CCGTGAAGAC CGGCATGGGG GGAAAGGCGG 
     2761 GGAATAAGGG TGCTGTGGGC ATCCGCTTCC AGCTCCACAG CACCAGTTTC TGCTTCGTCT 
     2821 GTAGCCACCT GACGGCTGGG CAGTCTCAGG TGAAGGAGAG GAATGAAGAC TACCGGGAGA 
     2881 TCACGCACAA ACTCTCCTTC CCTTCGGGGA GAAACATATT TTCACATGAT TACGTGTTTT 
     2941 GGTGTGGCGA TTTCAACTAC CGTATTGATC TTACTTACGA AGAAGTCTTC TATTTTGTTA 
     3001 AACGCCAAGA CTGGAAGAAA CTTATGGAAT TCGATCAGTT ACAGTTACAG AAATCAAGTG 
     3061 GAAAAATTTT TAAAGACTTT CATGAAGGAG CCGTTAACTT CGGACCCACC TACAAGTATG 
     3121 ACGTTGGATC AGCTGCCTAC GACACAAGTG ACAAGTGCCG TACCCCAGCC TGGACAGACA 
     3181 GGGTGCTGTG GTGGAGGAAG AAGCATCCAT ATGATAAAAC AGCTGGTGAA CTCAACCTTC 
     3241 TAGACAGCGA TCTAGACGGC GATCCCCAAA TCAGACACAC CTGGTCTCCA GGCACTCTGA 
     3301 AATACTACGG CCGTGCAGAG CTGCAGGCGT CTGATCACAG ACCTGTGCTG GCCATTGTGG 
     3361 AGGTGGAGGT TCAAGAGGTG GATGTAGGAG CCCGGGAGAG GGTCTTCCAG GAAGTGTCCT 
     3421 CTGTCCAAGG CCCGCTGGAT GCCACCGTTG TTGTAAACCT CCAGTCTCCA ACCCTAGAAG 
     3481 AGAAAAATGA ATTTCCAGAG GACCTGCGCA CAGAACTTAT GCAGACTTTG GGGAATTATG 
     3541 GGACGATTAT TCTAGTCAGG ATCAACCAAG GGCAGATGCT GGTGACGTTT GCAGACAGCC 
     3601 ACTCGGCTCT CAGTGTGCTG GATGTGGATG GTATGAAGGT GAAAGGCAGG GCCGTGAAGA 
     3661 TTCGACCAAA GACCAAAGAT TGGCTAGAAG GCCTGAGAGA GGAGCTTCTC CGGAAGCGAG 
     3721 ACAGCATGGC CCCTGTGTCT CCCACCGCCA ACTCCTGCTT GTTGGAGGAG AACTTTGACT 
     3781 TCTCGAGTCT GGACTATGAG TCCGAAGGGG ATGTTCTTGA AGAGGATGAA GACTATTTAG 
     3841 TGGATGGGTT TGGCCAGCCT GTAGTCTCAG ACAGTGAGCT CGGTGGAGAC AACTCTTCCG 
     3901 ACACCATGAG CTCCTTGACA CCCGCCAGCA AGTCTCCCGC CCTGGCTAAA AAGAAGCAAC 
     3961 ATCCAACATA CAAAGACGAC GCTCACCTGG TGACCTTAAA GCAGGAGCTG GAAGTTGCTG 
     4021 GGAATTTTCG CCACCGTTCT CCGAGCAGGT CCCTGTCCGT CCCCAATAGG CCTCGGCCAC 
     4081 CTCACCCACC ACAGAGACCC CCCCCTCCAA CTGGCTTAAT GGTGAAGAAG TCAGCCTCAG 
     4141 ACGCGTCCAT CTCTTCTGGC ACTCATGGAC AATATTCCAT CTTGCAGACA GCGAAACTTC 
     4201 TCCCAGGAGC ACCCCAGCAA CCACCCAAGG CTAGAACTGG AATAAGTAAA CCTTACAACG 
     4261 TCAAACAGAT CAAAACCACC AACGCTCAGG AGGCAGAAGC AGCTATCCGG TGTCTCCTGG 
     4321 AAGCTAGCGG AGGGGTCCCG GAATCAGCCC CAGGTGCCAT ACCCCTGAGA AACCAAGGGT 
     4381 CTTCTAAGCC AGAGGCCACC CTGGGGCCCC CAGCCCTGCC CCGCCGGCCT GCTCCAAGGG 
     4441 TTCCCACTAT GAAGAAACCA ACTTTGAGGA GGACAGGAAA GGTGTACTCG GGCATCTCCC 
     4501 AGTGCTTGCG TGAAGAGCTG CGCTCTGCTG CCTGCACCCC ACACGCAGTG AGTGCACAGG 
     4561 ACTGCGGAGA CCTTAACAAC AGATGGAGAA TGCCACGTTT CTCGCACTAT ATTCACACAA 
     4621 AAAAATGGAA GAACGTATCT CTCAGTTTTC AAGATCTCTG GCTAAAGTTT CGCAGAAGCG 
     4681 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//