LOCUS       MR218540                7755 bp    DNA     circular  SYN 
DEFINITION  Dis3 (Myc-DDK-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae) (Dis3)
ACCESSION   NM_028315
VERSION     NM_028315.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3912..4003 
                     /label="Myc-DDK"  
     misc_feature    4033..4618 
                     /label="PolyA signal"  
     misc_feature    4090..4109 
                     /label="Restriction Enzyme"  
     misc_feature    4894..5566 
                     /label="ColE1"  
     misc_feature    5996..6790 
                     /label="Kan/Neo"  
     misc_feature    6825..7127 
                     /label="SV40 ori"  
     misc_feature    7213..7690 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3894..3900 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3894 
                     /label="Dis3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCAGGTCC AAGACGTTCT TGAAGAAGAC CCGCGCGGGC GGCGTGGTGA 
     1081 AGATCGTGCG CGAGCACTAC CTGCGGGATG ACATCGGCTG CGGCGCGCCG GCTTGCTCGG 
     1141 CCTGCGGCGG GGCGCACGCG GGCCCGGCCC TGGAGCTGCA GCCCCGGGAC CAGGCGAGCA 
     1201 GCCTCTGCCC GTGGCCGCAC TACCTTCTGC CGGACACCAA TGTGCTGCTG CACCAGATTG 
     1261 ATGTCCTCGA ACACCCGGCC ATCAGAAATG TCATTGTGCT ACAAACAGTG ATGCAAGAAG 
     1321 TGAGAAACCG GAGCGCCCCC ATCTACAAGC GAATCAGGGA TGTGACCAAT AACCAGGAAA 
     1381 AGCATTTCTA TACCTTCACT AATGAGCACC ATAAAGAAAC CTACATCGAG CAAGAGCAGG 
     1441 GAGAGAATGC CAATGACAGG AATGACAGAG CCATCCGAGT CGCAGCGAAG TGGTACAACG 
     1501 AGCACCTGAA GAGGGTGGCA GCAGACAGTC AGCTGCAAGT TATCCTGATA ACCAATGACA 
     1561 GGAAGAACAA AGAGAAAGCT GTGCAAGAGG GGATACCAGC CTTCACGTGT GAAGAATACG 
     1621 TAAAGAGCCT GACTGCTAAC CCTGAACTTA TAGACCGTCT TGCTTACTTG TCCGATGAAA 
     1681 TGAATGAAAT AGAAAGTGGG AAAATAATAT TTTCAGAGCA TCTTCCCTTA AGCAAGCTCC 
     1741 AACAAGGCAT AAAATCTGGT TCCTATCTTC AAGGAACATT CAGAGCTAGC AGGGAAAATT 
     1801 ATTTGGAGGC TACAGTATGG ATTCATGGAG ACAAAGAAGA GGAAAAAGAG ATACTTATAC 
     1861 AGGGAATTAA GCATCTAAAC AGAGCTGTGC ATGAAGACAT TGTGGCCGTG GAGCTACTGC 
     1921 CCAGGAGCCA GTGGGTGGCA CCGTCTTCCG TGGTTTTAGA CGATGAAGGT CAAAATGAAG 
     1981 ACGATGTGGA GAAAGATGAG GAGAGAGAAC TCCTGCTTAA GACTGCTGTA AGTGAAAAAA 
     2041 TGTTACGGCC TACAGGTCGA GTTGTGGGGA TAATAAAAAG GAACTGGAGA CCGTATTGTG 
     2101 GCATGCTTTC CAAGTCTGAT ATTAAGGAGT CAAGAAGACA TCTCTTTACA CCCGCTGATA 
     2161 AGAGAATTCC ACGAATTCGG ATAGAAACCA GACAGGCTTC TGCGTTAGAA GGACGGAGAA 
     2221 TTATTGTCGC TATTGATGGT TGGCCTAGAA ATTCTAGATA TCCAAATGGA CACTTTGTAA 
     2281 AGAATTTAGG CGATGTTGGA GAGAAGGAGA CAGAAACGGA AGTGTTGCTG CTCGAGCACG 
     2341 ATGTTCCTCA TCAGCCCTTT TCCCAGGCTG TGCTTAGCTT CCTGCCCAGG ATGCCCTGGA 
     2401 GCATTACTGA GGAGGACATG AAAAACCGAG AAGACCTGAG ACATCTGTGT GTTTGCAGTG 
     2461 TGGACCCTCC AGGGTGCACT GACATAGATG ACGCTCTGCA TTGTAGAGAG CTCAGCAATG 
     2521 GAAACTTGGA GGTTGGTGTT CATATTGCGG ATGTTAGCCA TTTTATCAGG CCAGGAAATG 
     2581 CGTTGGATCA AGAATCTGCA AGAAGAGGAA CAACTGTTTA TCTTTGTGAA AAGAGGATTG 
     2641 ACATGGTTCC AGAGTTGCTC AGCTCCAACC TCTGTTCCTT AAGATCCAAC GTTGACAGGT 
     2701 TGGCATTTTC CTGTATTTGG GAAATGAATC ATAATGCTGA AATATTAAAA ACGAGATTTA 
     2761 CCAAAAGTGT CATTAATTCA AAGGCTTCTC TTACGTACGC GGAAGCACAG ATGAGAATTG 
     2821 ATTCGGCGGC TATGAATGAT GATATTACCA CTAGTCTCCG TGGACTCAAT CAGCTGGCTA 
     2881 AAATTCTAAA AAAGGGAAGG ATTGAAAAGG GGGCTTTGAC TCTGTCTTCT CCAGAGATCC 
     2941 GATTCCACAT GGACAGTGAA ACCCACGACC CAATCGACCT GCAGACGAAA GAGCTGAGAG 
     3001 AAACAAATTC CATGGTGGAA GAATTTATGT TACTTGCTAA TATTTCTGTC GCAAAAAAAA 
     3061 TTCATGAAGA GTTTTCTGAA CATGCTCTGC TTCGGAAACA CCCAGCTCCG CCTCCCTCGA 
     3121 ATTATGACAT TCTTGTTAAG GCTGCAAAGT CCAAGAATTT GCAAATTAAG ACTGATACAG 
     3181 CCAAATCTTT GGCCGACTCT TTGGACCGGG CTGAATCTCC GGATTTCCCA TACCTGAATA 
     3241 CTCTATTAAG GATACTGGCC ACTCGCTGTA TGATGCAAGC TGTGTACTTC TGCTCTGGGA 
     3301 TGGATAATGA TTTTCATCAC TATGGCTTAG CCTCCCCCAT ATACACACAT TTCACCTCTC 
     3361 CTATCAGAAG ATATGCAGAC ATAATTGTCC ATCGGCTATT AGCTGTGGCG ATTGGGGCTG 
     3421 ACTGTACTTA CCCTGAGTTG ACAGACAAAC ACAAGCTTTC AGATATATGT AAAAACCTCA 
     3481 ATTTCCGGCA TAAAATGGCT CAGTATGCCC AGCGTGCTTC GGTGGCTTTT CATACACAGT 
     3541 TGTTTTTCAA AAGCAAAGGA ATAGTAAGTG AAGAAGCCTA TATTCTCTTT GTAAGGAAAA 
     3601 ATGCAATTGT GGTGTTAATT CCAAAGTATG GCTTAGAAGG TACAGTTTTC TTTGAAGAAA 
     3661 AAGATAAACC AAAGCCACGC CTTGCTTACG ATGATGAGAT CCCTTCCCTG AGAATAGAAG 
     3721 GTACAGTGTT CCATGTGTTT GATAAGGTTA AAGTGAAGAT CACATTAGAT TCATCAAATC 
     3781 TTCAACATCA GAAAATCCGC ATGGCCCTTG TAGAACCACA GATCCCAGGA ATAAATATTC 
     3841 CTCCTAATGT TGCAGACAAG GCTCTTACTG CACCAGGGGG AAAGAAGAGG AAGCTTGAGA 
     3901 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3961 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4021 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4081 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4141 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4201 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4261 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4321 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4381 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4441 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4501 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4561 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4621 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4681 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4741 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4801 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4861 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4921 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4981 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5041 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5101 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5161 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5221 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5281 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5341 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5401 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5461 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5521 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5581 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5641 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5701 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5761 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5821 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5881 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5941 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6001 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6061 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6121 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6181 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6241 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6301 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6361 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6421 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6481 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6541 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6601 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6661 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6721 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6781 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6841 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6901 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6961 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7021 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7081 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7141 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7201 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7261 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7321 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7381 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7441 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7501 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7561 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7621 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7681 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7741 TTTAACGCGA ATTTT
//