LOCUS       MR218517                8130 bp    DNA     circular  SYN 
DEFINITION  Fmnl2 (Myc-DDK-tagged) - Mouse formin-like 2 (Fmnl2)
ACCESSION   NM_172409
VERSION     NM_172409.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4287..4378 
                     /label="Myc-DDK"  
     misc_feature    4408..4993 
                     /label="PolyA signal"  
     misc_feature    4465..4484 
                     /label="Restriction Enzyme"  
     misc_feature    5269..5941 
                     /label="ColE1"  
     misc_feature    6371..7165 
                     /label="Kan/Neo"  
     misc_feature    7200..7502 
                     /label="SV40 ori"  
     misc_feature    7588..8065 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4269..4275 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4269 
                     /label="Fmnl2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACGCG GGGAGCATGG ACTCGCAGCA GACCGATTTC AAGGCGCACA 
     1081 ACGTGCCATT GAAGCTGCCG ATGCCCGAGC CAGGTGAACT GGAGGAGCGC TTTGCCATCG 
     1141 TGCTGAATGC TATGAACCTA CCTCCTGACA AAGCCAGGCT ACTGCGGCAG TATGACAATG 
     1201 AGAAAAAATG GGAGCTGATC TGTGATCAGG AGCGATTCCA GGTGAAGAAC CCTCCCCATA 
     1261 CGTACATACA GAAGCTCAAA GGCTACCTGG ACCCAGCTGT AACCAGGAAG AAATTCAGAA 
     1321 GGCGTGTTCA AGAATCTACA CAAGTGCTAA GAGAACTGGA AATTTCATTA AGAACCAACC 
     1381 ACATTGGATG GGTCAGAGAG TTTTTGAATG AAGAAAACAA AGGCCTTGAT GTTCTTGTGG 
     1441 AGTATTTGTC TTTTGCACAG TATGCAGTCA CTTTTGACTT TGAAAGTGTG GAAAGTACCA 
     1501 TGGAGAGTAC GGTGGACAAA TCAAAGCCCT GGAGCAGGTC CATCGAGGAC CTGCACAGAG 
     1561 GGAGCAACCT GCCCTCACCT GTGGGCAACA GTGTGTCCCG CTCTGGAAGA CATTCTGCAC 
     1621 TTCGATATAA TACTTTGCCA AGCAGAAGAA CCCTGAAAAA TTCAAGATTA GTGAGTAAGA 
     1681 AGGATGATGT TCACGTTTGT ATCATGTGTT TACGAGCCAT CATGAATTAC CAGTATGGCT 
     1741 TCAACATGGT CATGTCTCAT CCACACGCCG TCAATGAGAT TGCACTAAGT CTGAACAACA 
     1801 AAAATCCCAG AACAAAAGCT CTTGTCTTGG AGCTGTTGGC AGCCGTTTGT CTTGTCAGAG 
     1861 GCGGGCATGA AATAATTTTA TCAGCTTTTG ATAACTTCAA GGAGGTCTGC GGAGAAAAGC 
     1921 AGCGTTTTGA GAAGCTGATG GAACATTTCA GGAATGAAGA TAATAACATA GATTTTATGG 
     1981 TGGCCTCTAT GCAGTTTATT AACATTGTAG TCCATTCAGT AGAAGACATG AACTTCAGAG 
     2041 TTCATCTACA ATATGAATTC ACCAAATTAG GCCTGGATGA ATACTTGGAT AAGCTGAAAC 
     2101 ACACAGAGAG CGACAAGCTG CAAGTACAGA TTCAGGCTTA CCTGGACAAT GTGTTTGATG 
     2161 TGGGAGCTCT GTTGGAAGAC GCAGAAACCA AGAATGCTGC CTTGGAGCGG GTGGAGGAGC 
     2221 TGGAAGAAAA CATCTCTCAT TTGTCTGAAA AGCTGCAGGA CACGGAGAAC GAAGCCATGT 
     2281 CCAAGATCGT GGAACTGGAA AAGCAACTTA TGCAGAGGAA TAAGGAACTG GATGTTGTCC 
     2341 GGGAAATCTA CAAAGATGCA AATACCCAAG TTCACACGTT AAGAAAAATG GTCAAAGAAA 
     2401 AGGAAGAAGC CATTCAAAGA CAGTCTACCC TGGAAAAAAA GATTCACGAA CTGGAGAAAC 
     2461 AAGGGACCAT TAAAATTCAG AAGAAAGGGG ATGGGGACAT TGCCATACTG CCAGTCATGG 
     2521 CCTCTGGCAC GTTGTCCACG GGGTCAGAAC TAGCAGTGGG TAACTATGTG GGATCAGTTC 
     2581 CAGGGGCCAC CACCTCAGGA CCCTCAGTCC CTCCTCCCCC ACCACTACCT CCATCATCAG 
     2641 ACACATCTGA AGCAGCACAA AATGGAACAG CATCGCCACC TATGTCCCCA CCTCCTCCCC 
     2701 CACCCCCTCC ACCCCCTCCC CCACCCCCTC CACCCCCTCC ACTCCCAGGC CCTGCAGCTG 
     2761 AGACTTCACC AGCTCCTCCT CTGCCACCAC CCCCACCTCC CTCTGCACCC CCGCTGCCTG 
     2821 GGACTTCTTC ACCTACAGTG GTTTTCAACT CAGGATTAGC AGCTGTGAAA ATTAAGAAGC 
     2881 CAATCAAGAC GAAGTTCCGA ATGCCAGTGT TTAACTGGGT CGCCCTGAAG CCCAATCAGA 
     2941 TCAACGGCAC AGTCTTCAAT GAAATCGATG ATGAGCGGAT CCTAGAGGAC TTAAATGTGG 
     3001 ACGAATTTGA AGAAATATTC AAGACAAAAG CCCAAGGTCC TGCCATTGAT CTTTCTTCAA 
     3061 GCAAGCAGAA AATAACACAG AAGGCATCAA GTAAAGTGAC ATTATTAGAA GCAAATCGAG 
     3121 CCAAAAATCT TGCCATAACT TTAAGGAAAG CTGGGAAGTC TGCGGATGAG ATATGTAAAG 
     3181 CTATTCATGT GTTTGACTTG AAGACGCTAC CGGTTGACTT TGTTGAGTGT TTGATGAGGT 
     3241 TCTTGCCAAC TGAGAATGAG GTCAAAGTGC TTCGGCTGTA TGAGCGAGAG AGGAAGCCCT 
     3301 TGGAGAACTT GTCAGACGAG GACCGTTTCA TGATGCAGTT CAGTAAGATT GAGCGGCTGC 
     3361 TGCAGAAGAT GACCATCATG GCCTTCATTG GGAACTTCAC AGAGAGCATT CAGATGCTGA 
     3421 CTCCTCAACT TCACGCAATT ATAGCAGCAT CCGTCTCTAT CAAGTCATCT CAAAAGCTCA 
     3481 AGAAGATTCT GGAGATCATC TTGGCCCTTG GAAACTATAT GAATAGCAGC AAACGAGGAG 
     3541 CAGTCTATGG ATTTAAACTT CAGAGTTTAG ATCTGCTCTT AGATACAAAG TCGACAGACC 
     3601 GAAAGCAAAC ACTGTTGCAC TATATATCAA ATGTGGTAAA AGAAAAATAT CAACAAGTTA 
     3661 CTCTGTTTTA TAATGAGCTT CATTACGTGG AAAAAGCTGC TGCAGTCTCC CTGGAGAACG 
     3721 TTCTGCTGGA TGTGAAGGAG CTGCAGAGGG GGATGGATTT AACCAAGAGA GAGTATACCA 
     3781 TGCATGACCA CAACACCCTG CTGAAAGAGT TCCTCCTTCA CAACGAGGGC AAGCTGAAGA 
     3841 AACTGCAGGA GGACGCGAAG ATTGCTCAGG ATGCCTTTGA TGATGTGGTG AAATATTTTG 
     3901 GCGAAAATCC CAAGACAACT CCACCCTCCG TGTTCTTCCC TGTCTTTGTT CGATTTGTGA 
     3961 AAGCCTACAA GCAAGCAGAA GAGGAAAATG AGCTGAGGAA AAAGCAGGAA CAAGCTCTTA 
     4021 TGGAAAAACT GCTAGAGCAA GAAGCCCTGA TGGAGCAGCA GGATGCAAAG TCTCCTTCCC 
     4081 ATAAATCAAA GAGGCAGCAG CAAGAGTTAA TTGCGGAATT AAGAAGGCGA CAAGTTAAAG 
     4141 ATAACAGACA TGTATACGAG GGAAAAGATG GCGCCATTGA AGACATTATC ACAGCCTTAA 
     4201 AGAAGAATAA TATCACTAAA TTTCCAAATG TTCACTCGAG GATCTTAGAA ACCAACCCTA 
     4261 CAGACGAGCC GATGCGGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4321 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4381 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4441 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4501 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4561 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4621 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4681 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4741 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4801 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4861 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4921 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4981 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5041 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5101 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5161 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5221 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5281 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5341 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5401 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5461 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5521 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5581 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5641 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5701 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5761 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5821 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5881 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5941 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6001 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6061 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6121 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6181 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6241 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6301 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6361 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6421 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6481 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6541 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6601 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6661 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6721 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6781 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6841 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6901 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6961 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7021 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7081 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7141 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7201 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7261 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7321 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7381 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7441 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7501 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7561 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7621 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7681 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7741 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7801 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7861 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7921 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7981 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8041 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8101 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//