LOCUS       MR218342                7137 bp    DNA     circular  SYN 
DEFINITION  Ppig (Myc-DDK-tagged) - Mouse peptidyl-prolyl isomerase G (cyclophilin G) (Ppig)
ACCESSION   NM_001081086
VERSION     NM_001081086.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3294..3385 
                     /label="Myc-DDK"  
     misc_feature    3415..4000 
                     /label="PolyA signal"  
     misc_feature    3472..3491 
                     /label="Restriction Enzyme"  
     misc_feature    4276..4948 
                     /label="ColE1"  
     misc_feature    5378..6172 
                     /label="Kan/Neo"  
     misc_feature    6207..6509 
                     /label="SV40 ori"  
     misc_feature    6595..7072 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3276..3282 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3276 
                     /label="Ppig"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGATAAAG GTCCAGCGTC CTCGGTGTTT TTTTGACATT GCCATTAATA 
     1081 ATCAACCTGC TGGAAGAGTT GTCTTTGAAT TATTTTCCGA TGTATGTCCC AAAACGTGTG 
     1141 AGAACTTTCG CTGCCTTTGC ACAGGTGAAA AAGGGACAGG GAAATCAACT CAGAAGCCAT 
     1201 TACATTATAA GAGCTGTCTC TTTCATAGAG TTGTCAAGGA TTTCATGGTT CAGGGTGGTG 
     1261 ACTTCAGTGA AGGGAATGGA AGAGGAGGAG AATCTATTTA TGGAGGATTC TTTGAAGATG 
     1321 AGAGTTTTGC TGTTAAACAC AACAAAGAGT TTCTCTTATC AATGGCCAAC AGAGGGAAGG 
     1381 ATACAAATGG CTCTCAGTTC TTTATAACAA CAAAACCAAC TCCTCATTTA GATGGGCATC 
     1441 ATGTTGTTTT TGGGCAAGTG ATCTCTGGTC AGGAAGTTGT GAGAGAGATT GAAAACCAGA 
     1501 AAACAGATGC CGCTAGCAAA CCATTTGCTG AAGTTCGGAT ACTCAGTTGC GGAGAGCTGA 
     1561 TTCCAAAGTC TAAGGTTAAG AAAGAAGAAA AGAAAAGGCA TAAGTCCTCG TCCTCCTCCT 
     1621 CCTCCAGTGA CTCAGACAGC TCAAGCGATT CTCAGTCCTC CTCTGAGTCT TCTGATTCTG 
     1681 AAAGCGCTTC CGAAGAAAAA TCCAGAAAAA GGAAAAAGAA ACATAGGAAA AATTCCCGAA 
     1741 AGCATAAGAA GGAGAAGAAG AAGCGAAAGA AAAGCAAGAA AAGCCCATCT AGTGAAAGTG 
     1801 AAGCAGAAAA TGTTGATGCA CAGCCCCAGT CTACTGTCCG TCCAGAAGAG ATCCCTCCTA 
     1861 TACCAGAAAA TAGATTTCTA ATGAGAAAAA GTCCTCCTAA AGCCGATGAC AAGGAGAGGA 
     1921 AAAACAGAGA GAGAGAAAGA GAAAGAGAGT GTAATCCACC TAACTCCCAG CCTGCTTCAT 
     1981 ACCAGCGACG ATTCTTAGTT ACTAGGTCTG GCAGGAAAAT TAAAGGAAGA GGACCGAGGC 
     2041 GTTATCGAAC TCCTTCCAGA TCCAGATCGA GGGATCGATT CAGACGAAGT GAGACTCCTC 
     2101 CACATTGGAG ACAAGAGATG CAGAGAGCTC AGAGAATGAG GGTGTCAAGT GGTGAAAGAT 
     2161 GGATCAAAGG GGATAAGAGT GAGTTAAATG AAATAAAAGA AAATCAAAGG AGCCCAGTTA 
     2221 GAGTAAAAGA GAAAAAAATA ACAGATCACA GACACATGTC TGAGAGTCCA AACAGAAAAG 
     2281 TAGAAAAGGA AAAGAAAGCT AAAGACCATA AATCTGAAAG CAAAGAGAGA GACATCAGAA 
     2341 GAAATTCAGA AAAAGATGAT AAATATAACA AAAACAAAGT GAAGAAAAGG GGCAAATCCA 
     2401 AGAGTAGGAG CAAGAGCAAG GAGAGATCCA AGAGTAAGGA GCGAGACTCG AAGCACAGCA 
     2461 GACACGAAGA CAAGAGGGTG AGGTCAAGAA GTAAAGAAAG GGATCATGAG ACTACTAAAG 
     2521 AAAAAGAAAA ACCACTGGAT CCTAAAGGGA AAGATCAGGA AAGGAGTAGA AGCAAAGAGA 
     2581 ACTCCAAACA GGTAGAATCA AAAAGTAATG AACATGATCA TAGCAAAAGT AAAGAAAAGG 
     2641 ATAGACGTGC ACAGTCCAGG AGTAGAGAAC GGGATCTGAC TAAAAGTAAA CACAGTTACA 
     2701 ACAGTAGAAC AAGGGAGCGG AGCAGAAGTA GGGACAGGAG CAGAAGAGTG CGGTCCAGAA 
     2761 GCCACGACAG AGATCGCAGC AGAAGCAAGG AGTACCATAG ATACAGAGAA CAGGAGTATA 
     2821 GGAGAAGAGG AAGGTCACGA AGCCGGGATC GAAGAACGCC AGGACGATCG AGAAGTAAAG 
     2881 ATAGGAGGAG AAGAAGGAGG GATTCCCGGA GCTCAGAGAG AGAAGAAAGT CAAAGTAGAA 
     2941 ACAAAGATAA ATATAGAAGC CAAGAAAGTA AGAGTTCACA CAGAAAAGAA AATTCTGAGG 
     3001 GTGAGAAAAG AACGTACTCT AAAAGTCGTG ATCATAATAG CTCAAGTAAT AACAGGGAAA 
     3061 AAAAGGCTGA TAGAGAGCAA AGTCCAGTGT CAAAAACAAA GCAAAGCAGT CAGGACAATG 
     3121 AAGTGAAGTC CTCCACATTA AAAAATCAAG AGGACGAAAA GACCAGATCC CCGGTGGAAA 
     3181 AAGAAAACCA AAAATCAAAG GGTCAAGAAA ATGACCATGT ACATGACAAA AATAAAAAAT 
     3241 GTGATCATGA ATCAAGCCCT GGAACAGATG AAGACAAAAG TGGAACGCGT ACGCGGCCGC 
     3301 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3361 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3421 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3481 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3541 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3601 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3661 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3721 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3781 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3841 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3901 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3961 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4021 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4081 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4141 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4201 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4261 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4321 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4381 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4441 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4501 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4561 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4621 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4681 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4741 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4801 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4861 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4921 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4981 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5041 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5101 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5161 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5221 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5281 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5341 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5401 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5461 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5521 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5581 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5641 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5701 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5761 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5821 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5881 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5941 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6001 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6061 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6121 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6181 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6241 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6301 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6361 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6421 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6481 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6541 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6601 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6661 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6721 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6781 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6841 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6901 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6961 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7021 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7081 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//