LOCUS       MR218327                7383 bp    DNA     circular  SYN 
DEFINITION  Rasa3 (Myc-DDK-tagged) - Mouse RAS p21 protein activator 3 (Rasa3)
ACCESSION   NM_009025
VERSION     NM_009025.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3540..3631 
                     /label="Myc-DDK"  
     misc_feature    3661..4246 
                     /label="PolyA signal"  
     misc_feature    3718..3737 
                     /label="Restriction Enzyme"  
     misc_feature    4522..5194 
                     /label="ColE1"  
     misc_feature    5624..6418 
                     /label="Kan/Neo"  
     misc_feature    6453..6755 
                     /label="SV40 ori"  
     misc_feature    6841..7318 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3522..3528 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3522 
                     /label="Rasa3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGGAG GAAGAGGGAC TGCGGGTCTT CCAGAGCGTG AGGATCAAGA 
     1081 TTGGTGAAGC CAAGAATCTT CCTTCCTACC CGGGGCCAAA CAAGATGAGG GACTGCTACT 
     1141 GCACTGTGAA CCTGGACCAG GAGGAGGTTT TCCGGACCAA AATTGTCGAG AAGTCACTCT 
     1201 GCCCCTTTTA CGGAGAGGAC TTTTACTGTG AAATCCCTCG GAGTTTCCGC CACCTGTCCT 
     1261 TTTACATTTT TGATAGAGAT GTTTTCCGAA GGGACTCCAT CATAGGCAAG GTGGCCATCC 
     1321 AGAAGGAAGA CTTGCAGAGG TACCACAACA GGGACACATG GTTCCAGCTG CAGCACGTGG 
     1381 ACGCAGACTC AGAGGTGCAG GGCAAGGTCC ACCTGGAGCT GAGACTAAGT GAGGTCATTA 
     1441 CGGACACTGG TGTCGTCTGC CACAAACTCG CTGCACGCAT CTTCGAGTGC CAGGGTCTCC 
     1501 CCATTGTGAA CGGGCAGTGT GACCCTTACG CCACAGTGAC GCTGGCCGGA CCCTTCAGGT 
     1561 CTGAAGCAAA GAAAACGAAG GTGAAGAAGA AAACCAACAA CCCCCAGTTT GACGAGGTGT 
     1621 TTTATTTTGA GGTGACCAGA CCCTGCAGCT ACAGCAAAAA GTCCCACTTT GACTTTGAGG 
     1681 AAGAGGACGT GGACAAACTT GAAATCCGAG TTGACCTCTG GAACGCCAGC AACCTGAAGT 
     1741 TTGGGGATGA GTTTCTCGGG GAACTGAGGC TTCCTCTCAA GATTCTGCGA CACTCGAGCT 
     1801 CTTATGAAGC CTGGTACTTC CTCCAGCCCC GAGACAACGG CAATAAGAGC CTGAAGCCAG 
     1861 ATGACTTGGG GTCTCTGAGG TTAAATGTCG TTTATACAGA AGACCATGTC TTCTCCTCTG 
     1921 AGTACTACAG CCCACTGCGT GACCTGTTAC TGAAGTCTGC AGATGTGGAG CCTGTCTCAG 
     1981 CCTCAGCAGC TCACATCCTG GGTGAGGTGT GCAGAGACAA GCAAGAGGCA GCCATCCCGC 
     2041 TGGTACGGCT CCTGCTGCAC TATGGCAGGG TAGTGCCCTT CATCAGTGCC ATCGCTAGCG 
     2101 CAGAGGTGAA GAGGACCCAG GACCCCAATA CCATCTTCCG AGGAAACTCA CTGACATCCA 
     2161 AGTGCATAGA TGAGACGATG AAGCTGGCAG GCATGCACTA TCTCCATGTG ACCCTGAAGC 
     2221 CCACCATTGA GGAGATCTGC CAGAGCCACA AGTCCTGTGA GATCGACCCT GTGAAACTGA 
     2281 AAGACGGCGA AAACCTCGAG AACAACATGG AGAGCCTGAG GCAGTATGTG GATCGCATCT 
     2341 TCACTGTCAT CACCAAGTCC GGGGTGAGCT GCCCCACCGT CATGTGTGAC ATCTTTTTCT 
     2401 CCTTGCGGGA GGCGGCTGCC AAGCGCTTCC AAGATGACTT GGATGTGAGG TACACGGCCG 
     2461 TGAGCAGCTT CATCTTCCTC AGGTTCTTCG CCCCTGCCAT CCTGTCCCCA AACCTTTTCC 
     2521 AGCTGACACC CCACCACACG GATCCACAGA CTTCTAGAAC CCTGACACTT ATCTCAAAGA 
     2581 CGATCCAGAC CCTCGGCAGC TTGTCCAAGT CCAAGTCTGC CAGTTTTAAG GAGTCGTACA 
     2641 TGGCGACATT CTATGAATTC TTCAATGAGC AGAAGTATGC AGATGCTGTA AAAAATTTTC 
     2701 TGGATTTGAT CTCATCCTCG GGGAGAAGGG ACCCCAAGAG CATAGAGCAG CCCATCCTGC 
     2761 TTAAAGAAGG GTTCATGATC AAGCGGGCCC AGGGAAGGAA ACGGTTTGGA ATGAAGAATT 
     2821 TCAAGAAGAG GTGGTTTCGC CTGACGAACC ACGAGTTCAC CTACCAGAAA AGCAAAGGTG 
     2881 ATCAGCCACT CTGCAACATC CCCATCGAGA ACATCTTGGC TGTGGAGAGG CTAGAGGAGG 
     2941 AGTCCTTCCG AATGAAAAAC ATGTTCCAGG TCATCCAGCC AGAGCGTGCC CTGTACATCC 
     3001 AGGCCAACAA CTGTGTGGAG GCCAAGGACT GGATCGACAT CCTCACCAAA GTGAGCCAGT 
     3061 GCAACCAGAA GCGGCTCACC GTCTTCCACC CGTCGGCCTA CCTGAACGGC CACTGGCTCT 
     3121 GCTGCAGGGC CTCCTCAGAC ACGGCTGCTG GCTGCACTCC CTGCACTGGT GGGCTCCCGG 
     3181 CCAACATCCA GCTGGACATT GATGGGGACC GTGAAACAGA GCGCATCTAC TCTCTCTTTA 
     3241 ACCTGTACAT GGGCAAGCTG GAAAAGATGC AGGAGGCCTG CGGCAGCAAG TCTGTGTATG 
     3301 ACGGCCCTGA GCAGGAGGAG TACTCAACGT TCGTCATCGA CGACCCCCAG GAGACGTACA 
     3361 AGACGCTGAA GCAGGTCATC GCTGGGGTGG GGACCCTTGA GCAGGAGCAC GCACAGTACA 
     3421 GGAGAGACAA GTTCAAGAAG ACGAGATACG GGAGCCAGGA GCACCCTATT GGAGACAAGA 
     3481 GCTTCCAGAA CTACATCCGG CAGCAGTCTG AGATCTCCAC CCATTCCATT ACGCGTACGC 
     3541 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3601 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3661 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3721 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3781 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3841 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3901 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3961 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4021 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4081 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4141 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4201 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4261 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4321 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4381 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4441 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4501 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4561 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4621 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4681 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4741 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4801 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4861 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4921 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4981 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5041 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5101 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5161 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5221 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5281 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5341 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5401 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5461 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5521 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5581 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5641 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5701 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5761 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5821 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5881 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5941 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6001 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6061 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6121 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6181 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6241 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6301 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6361 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6421 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6481 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6541 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6601 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6661 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6721 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6781 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6841 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6901 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6961 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7021 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7081 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7141 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7201 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7261 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7321 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7381 TTT
//