LOCUS       MR218326                8151 bp    DNA     circular  SYN 
DEFINITION  Fmnl1 (Myc-DDK-tagged) - Mouse formin-like 1 (Fmnl1), transcript variant 1
ACCESSION   NM_019679
VERSION     NM_019679.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4308..4399 
                     /label="Myc-DDK"  
     misc_feature    4429..5014 
                     /label="PolyA signal"  
     misc_feature    4486..4505 
                     /label="Restriction Enzyme"  
     misc_feature    5290..5962 
                     /label="ColE1"  
     misc_feature    6392..7186 
                     /label="Kan/Neo"  
     misc_feature    7221..7523 
                     /label="SV40 ori"  
     misc_feature    7609..8086 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4290..4296 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4290 
                     /label="Fmnl1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACGCG GCTGGCAGCG CCGAACAGCC CGCGGGCCCC ACCGCGTCGC 
     1081 CCCCGAAGCA GCCAGCCGTC CCCAAGCAAC CAATGCCGGC GGCCGGGGAG CTGGAGGAGA 
     1141 GGTTCACGCG GGTCTTGAAC TGTATGAACT TGCCTCCAGA CAAGGTCCAG CTGCTGAGCC 
     1201 AGTATGACAA TGAGAAGAAA TGGGAGCTCA TCTGTGACCA GGAGCGGTTT CAAGTCAAGA 
     1261 ATCCCCCTGC AGCCTACATC CAGAAGCTGA AGAGCTACCT GGATACCGGT GGGGTCAGCC 
     1321 GAAAGGTAGC ATCTGATTGG ATGTCCAACC TGGGGTTTAA GAGGCGAGTT CAGGAGTCTA 
     1381 CACAGGTGCT GCGGGAACTG GAGACTTCCC TGAGGACAAA CCACATTGGG TGGGTGCAGG 
     1441 AGTTCCTCAA TGAAGAGAAC CGGGGTCTGG ATGTCCTGCT TGAGTACCTG GCCTTTGCCC 
     1501 AGTGCTCTGT TGCATATGAC ATGGAAAGCA CAGACAGTGT GGCCTCTGGT GCAGAGAAGA 
     1561 GCAAGCCCCT GGACCAGTCT GTGGAAGACC TCAGCAAGGC ACCTCCCTCA TCTGTGCCCA 
     1621 AAAGTCGCCT GACCATCAAG CTGACCCCTG CCCACAGCAG GAAGGCGCTG AGGAATTCCC 
     1681 GAATCGTCAG CCAGAAGGAT GATGTCCACG TGTGCATCAT GTGTCTAAGA GCCATCATGA 
     1741 ACTACCAGTC CGGCTTCAGC CTTGTTATGA ACCACCCAGC CTGTGTCAAT GAGATCGCTC 
     1801 TAAGCCTTAA CAACAAGAGC CCCAGAACCA AGGCTCTGGT ACTGGAGCTG CTGGCAGCTG 
     1861 TATGTCTCGT GCGGGGAGGA CACGACATCA TCCTTGCGGC CTTTGACAAC TTCAAGGAGG 
     1921 TTTGCGGGGA GCAGCACCGA TTTGAAAAGC TAATGGAGTA TTTCCGGCAC GAAGACAGCA 
     1981 ACATTGACTT CATGGTGGCT TGCATGCAAT TCATCAACAT TGTGGTGCAC TCTGTGGAGA 
     2041 ATATGAACTT CCGTGTCTTC CTGCAATATG AGTTCACTCA CCTGGGTCTG GACCTGTACT 
     2101 TGGAGAGGCT CCGGCTCACT GAGAGTGACA AGCTGCAGGT GCAGATCCAG GCGTATCTGG 
     2161 ACAACGTTTT TGATGTGGGG ACATTGTTGG AGGAGACAGA GACGAAGAAT GCAGTGTTGG 
     2221 AGCACATGGA GGAACTGCAG GAGCAGGTGG CAACGCTGAC AGAGCGGCTT CGGGACACAG 
     2281 AGAACGACTC CATGGCCAAG ATCGCTGAAC TGGAGAAGCA GCTAAGCCAG GCTCGCAAGG 
     2341 AATTGGAGAC CCTGAGGGAG CGCTTCAGCG AGTCGACCCC CATGGGCACT TCCAGACGAA 
     2401 TCCCTGAACC TGAGAAAGTG CCTGTCCCTA CCGTGGTGCG ACCCTCGGCT CTAGAACTGA 
     2461 AGGTGGAGGA GCTTGAGGAA AAGGGGTTAA TCCGCATCCT GCGGGGGCCG GGGGACGTCG 
     2521 TTTCCATCGA GATCCTTCCA GGCGCTGCGG CGACACCAAG CGGTGACGAC GCACAAGCTC 
     2581 CGAGGGTGTC CACTGATTCC CCTAGCACAG CAGAGTCAAT TCCCGAAGCA GCATCACCAC 
     2641 CGCCTCCGCC ACCTCCACCA CCACCTCCAC TACCTAACCT CCAGTCCCAG CAGGAAGCCC 
     2701 CTCCCTCCGC ACCCCCGCTG GCCCCGCCCC TCCCAGGCTG CGCAGAGCCG CCACCTGCAC 
     2761 CACCGTTGCC TGGAGACCTG CCTCCCCCAC CTCCGCCACC CCCACTTGGT ACTGATGGAC 
     2821 CAGTGCCACC GCCACCGCCA CCGCCTCCGG GAGGGCCCCC TGATATCCTT GGAGGACAAG 
     2881 GCCCAGATAT CGGCCCAGGG GTGAAAGCCA AGAAACCCAT CCAGACCAAG TTCCGGATGC 
     2941 CACTGCTTAA CTGGGTGGCG CTGAAACCCA GCCAGATCAC AGGCACTGTC TTCACGGAGC 
     3001 TCAATGACGA GAAGGTGCTC CAGGAGCTAG ACATGAATGA CTTTGAGGAA CACTTTAAGA 
     3061 CCAAATCCCA AGGTCCCTGC CTGGATATCA GCGCTCTGAA GGGGAAGGCC TCCCAGAAGG 
     3121 CCCCCACTAA GACAATACTC ATTGAAGCCA ACCGGGCCAA GAATTTGGCT ATCACCCTGC 
     3181 GCAAGGGCAA CCTAGGAGCA GACAGAATCT GCCAGGCCAT CGAGACGTAT GACCTACAGA 
     3241 CTCTCAGCTT GGATTTCTTG GAGCTGTTGA CCCGCTTCCT GCCCACGGAC TACGAGCGTA 
     3301 GCCTCATAGC CCGATTCGAG AAGGAACAGC GGCCGATGGA AGAGCTGTCA GAGGAGGACC 
     3361 GCTTCATGCT TCGCTTCAGT CGCATCCAGA GACTTCCGGA GCGCATGAAC ACACTCACCT 
     3421 TCCTGGGCAA CTTTCCAGAC ACGGCCCAGC TGCTCATGCC GCAACTGAAC GCCATCATTG 
     3481 CAGCCTCTAT GTCTATCAAG TCCTCTGACA AGCTTCGCCA GATCCTAGAG ATTGTCCTGG 
     3541 CTTTTGGCAA CTACATGAAC AGTAGCAAGC GTGGGGCAGC CTATGGTTTC CGACTTCAGA 
     3601 GTCTGGATGC GTTGTTGGAG ATGAAGTCGA CCGACCGGAA ACAGACGTTG TTGCACTACT 
     3661 TGGTGAAGGT CATTGCTGAG AAGTACCCAC AGCTCACGGG TTTTCATAGT GATCTGCACT 
     3721 TTTTGGACAA GGCAGGCTCA GTGTCCCTGG ACAGCGTACT GGGAGACGTG CGCTCCTTGC 
     3781 AGCGAGGTCT GGAGCTGACG CAGAGAGAGT TTGTGCGGCA AGATGACTGC CTGGTGCTCA 
     3841 AGGAGTTCCT AAGGGCCAAC TCTCCCACCA TGGACAAGTT GCTGGCAGAC AGCAAGACAG 
     3901 CTCAGGAGGC CTATGAGTCT GTGGTGGAGT ACTTTGGAGA AAATCCCAAG ACCACATCCC 
     3961 CCTCCATGTT CTTTTCCCTC TTCAGTCGCT TCACCAAGGC CTACAAGAAA GCGGAACAGG 
     4021 AGGTCGAACA GTGGAAGAAG GAAGCAGCTG CTGATACCTC AGGCAGGGAA GAGCCTCCAA 
     4081 CACCCAAGTC TCCACCCAAG GCCCGGCGAC AACAGATGGA CCTCATCTCT GAGCTAAAAC 
     4141 GGAAGCAGCA GAAAGAGCCA CTCATCTACG AGAGTGACCG AGATGGGGCC ATCGAGGACA 
     4201 TCATCACAGT GCTGAAGACA GTGCCCTTCA CTGCCCGCAC CGGCAAGCGG ACATCCCGGC 
     4261 TCCTCTGTGA GGCCAGCCTG GGAGAGGAGA TGACCCTTAC GCGTACGCGG CCGCTCGAGC 
     4321 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4381 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4441 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4501 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4561 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4621 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4681 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4741 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4801 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4861 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4921 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4981 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5041 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5161 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5221 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5281 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5341 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5461 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5521 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5581 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5641 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5761 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5821 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5881 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5941 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6001 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6061 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6121 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6181 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6241 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6301 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6361 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6421 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6481 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6541 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6601 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6661 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6721 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6781 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6841 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6901 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6961 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7021 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7081 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7141 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7201 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7261 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7321 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7381 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7501 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7561 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7621 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7681 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7741 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7801 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7861 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7921 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7981 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8041 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8101 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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