LOCUS       MR218291                7302 bp    DNA     circular  SYN 
DEFINITION  Smpd4 (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4), transcript variant 4
ACCESSION   NM_001164609
VERSION     NM_001164609.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3459..3550 
                     /label="Myc-DDK"  
     misc_feature    3580..4165 
                     /label="PolyA signal"  
     misc_feature    3637..3656 
                     /label="Restriction Enzyme"  
     misc_feature    4441..5113 
                     /label="ColE1"  
     misc_feature    5543..6337 
                     /label="Kan/Neo"  
     misc_feature    6372..6674 
                     /label="SV40 ori"  
     misc_feature    6760..7237 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3441..3447 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3441 
                     /label="Smpd4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTTCCCT CACCTACAAC AGCCTAGTTT CCTTCTGGCT AGCCTGAAAG 
     1081 CTGACTCTAT AAATAAGCCC TTTGCACAAC GTTGCCAAGA TTTGGTGAAA GTCATTGAGG 
     1141 ATTTTCCAGC CAAGGAATTG CATGCCGTCT TCCCATGGCT GGTGGAAAGC ATCTTTGGCA 
     1201 GCCTGGATGG TGTCCTTGTT GGTTGGAATC TCCGATGCCT ACAGGGACGT GTGAACCCTG 
     1261 TGGAGTACAG CACAGCGATG GAATTTCTAG ACCCAAGTGG TCCAATGATG AAGTTGGTTT 
     1321 ATAAACTTCA AGCTGAAGAC TATAACTTTG ATTTTCCTGT CTCCTGTCTT CCTGGCCCTG 
     1381 TGAAGGCATC CATTCAAGAG AATGTCCTCC CTGACAGTCC TCTGTACCAC AACAAAGTCC 
     1441 AGTTTCCCCC GACCGGAGGC CTTGGCCTGA ACCTGGCCCT CAATCCATTT GAATACTATA 
     1501 TGTTCTACTT TGCTTTGAGC CTCATCTCTC AAAAGCCAAT GTCTATGACC CTCCATGTCC 
     1561 GTACTTCGGA CTGTGCCTAT TTCACCCTGG TGGATAGGTA CCTATCATGG TTCCTACCCA 
     1621 CTGAAGGCAG CGTACCTCCA CCACTCTGCT CCAGTCCAGG AGGCTCCAGC CCCTCACCAG 
     1681 CTCCCAGAAC ACCAGCCATG CCCTTTGCCT CCTATGGCCT CCACACTAGC CTCTTGAAGC 
     1741 GACACATCTC TCATCAGACA TCTGTGAATG CAGACCCTGC ATCCCATGAG ATCTGGCGGT 
     1801 CAGAAACTCT CCTCCAGGTT TTTGTGGAAA TGTGGCTTCA TCATTACTCC CTGGAGATGT 
     1861 ACCAAAAAAT GCAGTCCCCT CATGCCAAGG AGTCCTTCAC GCCTACTGAA GAGCACGTGC 
     1921 TGGTGGTACG CCTGCTGCTG AAGCACCTGC ATGCCTTTGC CAACAGCCTG AAGCCAGATC 
     1981 AGGCTTCGCC ATCTGCACAC TCCCATGCCA CCAGTCCGCT GGAGGAATTC AAACGGGCCG 
     2041 CAGTGCCTCG CTTTGTCCAG CAGAAGCTGT ACGTCTTCCT GCAGCACTGT TTTGGCCACT 
     2101 GGCCTCTGGA TGCAACCTTC AGAGCTGTCT TGGAGATGTG GCTGAGCTAC CTGCAGCCAT 
     2161 GGAGGTATGC CCCTGAGAAA CAGGCTCAGG GCAGCGATCC CCAGCCACGG TGTGTGTCAG 
     2221 AGAAGTGCCT GGCTCCATGG TCACCCTATC AGCACAGAGG ACTGAGGGCA CCCTTCATCC 
     2281 AGGAAAACCT GCTGATGTAC ACCAAGCTGT TTGTGAGCTT TCTGAACCGT GCCCTACGCA 
     2341 CAGACCTTGT CAGCCCCAAG AATGCACTCA TGGTCTTCCG AGTGGCTAAG GTCTTTGCCC 
     2401 AGCCCAACCT GGCTGAGATG ATCCAGAAAG GTGAGCAGCT GTTCCTGGAG CCGGAGCTCA 
     2461 TCATCCCCCA CCGCCAACAC CGGCTCTTCA CAGTCACCAC CAGCTTCCTG TCACCATGGC 
     2521 CCCCTGTTGT GACAGATGCC TCCTTCAAGG TGAAGAGCCA TGTCTATAGC CTAGAGGGCC 
     2581 AAGACTGCAA ATATACCCCA ATGTTTGGTC CTGAAATCCG AACCCTGGTC TTGCGCCTTG 
     2641 CTCAGCTCAT CACACAGGCC AAGCAGACTG CCAAGTCCAT CTCTGATCAG TATGTGGAAA 
     2701 GCCCAACTGG CCGCTCCTTC CTGTCATGGC TGACCTTTGG CCTCACAGAC ACAAATAGCT 
     2761 GCTACCCAGC CAATGACCTG GATGAAATAG GTCAGGACAG CATCCGCAAG ACAGACGAGT 
     2821 ACTTAGAAAA GGCCCTGGAG TACCTGCGCC AGATATTCCG GCTCAGCGAA GCCCAGCTGG 
     2881 CCCAGCTCAC GCTTGCCCTG GGGAGTGCTC GGGACGAGAA TGGGAAGCAG CAGCTCCCAG 
     2941 ATTGCATTGT GGGGGAAGAA GGACTCATCC TTACACCCTT GGGCCGCTAC CAGATCATTA 
     3001 ATGGTCTGCG AAGGTTCGAG ATTGAGTACC AGGGAGACTT AGAGCTGCAG CCCATCCGGA 
     3061 GCTACGAAAT CACCAGTCTG GTCCGTGCAC TCTTCCGGCT GTCCTCTGCC ATCAACCGTA 
     3121 GATTTGCAGG CCAGATGGCA GCTCTGTGCT CCCGGAATGA CTTCCTTGGC AGCTTCTGTC 
     3181 GCTACCACCT CACTGAGCCT GCCTTGAGCA ACAGACATCT GCTGAGCCCT GTAGGGCGGA 
     3241 GGCAGGTCAC CAATCCTGCA CGGGGCCCCA GGCTCAGCCT CCGTTTCCTG GGCAGTTACC 
     3301 GGACACTGCT CCTGCTCCTG ATGGCCTTCT TTGTGGCTTC TCTGTTCTGC ATTGGGCCCC 
     3361 TGTCCTGCTC CTTGCTCCTG GTCCTAGGAT ACGTCCTCTA TGCCATAGCT ATGACACTGC 
     3421 TCACTGAACG GGGGAAGCTG CACCAGCTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3481 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3541 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3601 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3661 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3721 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3781 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3841 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3901 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3961 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4021 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4081 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4141 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4201 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4261 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4321 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4381 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4441 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4501 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4561 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4621 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4681 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4741 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4801 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4861 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4921 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4981 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5041 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5101 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5161 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5221 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5281 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5341 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5401 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5461 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5521 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5581 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5641 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5701 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5761 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5821 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5881 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5941 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6001 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6061 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6121 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6181 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6241 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6301 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6361 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6421 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6481 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6541 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6601 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6661 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6721 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6781 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6841 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6901 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6961 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7021 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7081 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7141 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7201 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7261 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//