LOCUS       MR218256                7533 bp    DNA     circular  SYN 
DEFINITION  Chsy3 (Myc-DDK-tagged) - Mouse chondroitin sulfate synthase 3 (Chsy3)
ACCESSION   NM_001081328
VERSION     NM_001081328.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3690..3781 
                     /label="Myc-DDK"  
     misc_feature    3811..4396 
                     /label="PolyA signal"  
     misc_feature    3868..3887 
                     /label="Restriction Enzyme"  
     misc_feature    4672..5344 
                     /label="ColE1"  
     misc_feature    5774..6568 
                     /label="Kan/Neo"  
     misc_feature    6603..6905 
                     /label="SV40 ori"  
     misc_feature    6991..7468 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3672..3678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3672 
                     /label="Chsy3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGTGCGC TCCCGCCGCC CATGGGTGAG CGTGGCATTG GGGTTGGTCC 
     1081 TGGGCTTCAC CGCCGCGTCC TGGCTCATCG CCCCCCGGGT GGCCGAGCTG AGCGAGAAGA 
     1141 GGCGACGCGG CTCCAGTCTT TGCTCCTACT ACGGCCGCTC GGCTACCGGG CCCCGCGCGG 
     1201 ACGCGCAGCA GCTGCTCCCC CAACCCCAGT CCCGGCCGCG GCTAGAGCAG TCGCCGCCCC 
     1261 CTGCCAGCCA CGAGCTCCCC GGTCCTCAGC AGCCGGAGGC GGCGCCCGGA GGTCCCAGTT 
     1321 TTCGGAGCAG CCCCTGGCAG CAGCCGGCTC TGTTGCCGCA GAGGAGGCGA GGACACACGC 
     1381 CCGAAGGTGC GACGGCGCTT CCCGGCGCTC CGGCTGCCAA AGGGGAACCA GAGGAGGAGG 
     1441 ATGGGGGCGC GGCTGACCCT CGGAAGGGTG GCCGGCCGGG GAGCAGCCAC AACGGCAGCG 
     1501 GGGACGGGGG TGCCGCTGTC CCGACCTCCG GACCCGGGGA CTTCCTGTAC GTGGGTGTGA 
     1561 TGACCGCACA GAAGTACCTG GGCAGTCGCG CGCTGGCCGC GCAGCGGACC TGGGCGCGCT 
     1621 TCATCCCTGG CCGCGTGGAG TTCTTTTCCA GTCAGCAATC TCCCAGTGCT GCGCTTGGCC 
     1681 AGCCCCCGCC ACCTTTGCCT GTCATCGCGC TGCCAGGGGT CGACGATTCC TACCCTCCCC 
     1741 AGAAAAAGTC CTTCATGATG ATCAAGTACA TGCACGACCA CTATCTGGAC AAGTATGAGT 
     1801 GGTTCATGCG CGCCGACGAC GATGTCTACA TCAAAGGTGA TAAGTTAGAA GAATTTCTAA 
     1861 GATCCCTAAA TAGCAGCAAG CCTCTCTACC TGGGACAGAC GGGCCTGGGC AATACTGAAG 
     1921 AACTTGGAAA GCTGGGGCTG GAGCCCGGGG AGAACTTCTG CATGGGAGGA CCTGGCATGA 
     1981 TCTTCAGCAG AGAGGTTCTC AGGCGGATGG TGCCTCATAT CGGCGAATGC CTCCGAGAGA 
     2041 TGTACACCAC ACACGAAGAC GTAGAAGTAG GAAGGTGTGT TCGCCGTTTC GGCGGGACGC 
     2101 AGTGTGTCTG GTCTTATGAG ATGCAGCAGC TGTTCCATGA AAACTACGAA CACAATCGCA 
     2161 AGGGTTACAT CCAAGACCTC CACAACAGCA AAATCCACGC AGCCATCACG CTCCATCCGA 
     2221 ACAAAAGGCC CGCGTACCAG TACAGACTTC ATAACTACAT GCTCAGCCGC AAGATCTCCG 
     2281 AGCTCCGCTA CCGCACCATC CAGCTCCACC GGGAGAGCGC TCTCATGAGC AAGCTCAGCA 
     2341 ACAGTGAAGT GAGCAAAGAG GACCAACAGC TGGGAAGGAC GCCGTCCTTC AACCACTTCC 
     2401 AGCCTCGGGA GAGAAATGAA GTCATGGAGT GGGAGTTCCT GACGGGGAAG CTGCTTTACT 
     2461 CAGCTGCAGA GAACCAGCCT CCTCGACAGA GCATCAACAG CATCCTAAGG TCAGCTCTGG 
     2521 ATGACACTGT CCTGCAGGTG ATGGAGATGA TCAACGAGAA TGCCAAGAGT AGGGGCCGAC 
     2581 TCATCGACTT CAAGGAAATT CAGTATGGCT ACCGCAGGGT TGATCCCATG CACGGGGTTG 
     2641 AGTACATATT GGACCTGCTA CTCCTGTACA AAAGGCACAA AGGAAGGAAA CTGACTGTGC 
     2701 CTGTGAGGCG CCATGCCTAT CTTCAGCAGC CATTTAGCAA GCCTTTCTTC AGAGAGGTGG 
     2761 AAGAACTCGA CGTCAACCGT CTGGTGGAGA GTATCAACAG CGGTACACAG TCATTCTCCG 
     2821 TTATATCCAA TTCTCTAAAA ATTCTCTCTT CTCTTCAAGA GGCCAAAGAC ATAGGAGGGC 
     2881 ACAATGAAAA GAAAGTACAC ATTCTCGTTC CACTCGTTGG AAGGTACGAC ATTTTCTTGA 
     2941 GATTCATGGA AAATTTTGAA AGTACGTGTC TTATCCCAAA GCAAAATGTC AAGCTTGTCA 
     3001 TCATCCTTTT CAGCAGGGAT GCTGGCCAAG AGTCCATCAA GCACATTGAG CTGATACAAG 
     3061 AATATCAGAG CAGGTATCCC AGTGCAGAAA TGATGCTCAT TCCCATGAAG GGAGAGTTTT 
     3121 CCAGAGGTCT TGGTCTTGAA ATGGCTTCTT CCCAGTTTGA CAATGACACA TTGCTGCTAT 
     3181 TTTGTGATGT TGACTTGATT TTCAGAGGAG ACTTCCTCCA ACGCTGTCGA GACAATACAG 
     3241 TTCAGGGACA ACAGGTATAT TACCCCATCA TCTTTAGCCA GTATGACCCA AAGGTCACCC 
     3301 ATATGAGAAA TCCTCCCACA GAGGGTGACT TTGTATTCTC AAAGGAAACT GGGTTTTGGA 
     3361 GAGACTATGG CTACGGAATC ACATGCATTT ACAAAAGCGA TCTACTGGGT GCAGGTGGAT 
     3421 TTGATACCTC AATACAAGGC TGGGGACTGG AAGATGTAGA TCTCTATAAT AAAGTCATCC 
     3481 TATCTGGCTT ACGGCCCTTC AGAAGTCAAG AAGTGGGAGT GGTGCATATT TTCCATCCTG 
     3541 TTCATTGTGA TCCTAACTTG GACCCTAAGC AGTATAAGAT GTGCTTAGGA TCCAAAGCAA 
     3601 GTACTTTTGC CTCAACCATG CAACTGGCTG AACTCTGGTT AGAAAAACAT TTGGGTGTCA 
     3661 GGGATAATCG AACTCTCTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3721 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3781 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3841 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3901 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3961 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4021 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4081 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4141 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4201 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4261 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4321 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4381 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4441 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4501 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4561 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4621 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4681 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4741 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4801 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4861 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4921 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4981 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5041 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5101 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5161 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5221 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5281 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5341 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5401 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5461 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5521 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5581 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5641 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5701 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5761 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5821 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5881 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5941 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6001 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6061 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6121 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6181 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6241 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6301 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6361 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6421 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6481 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6541 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6601 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6661 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6721 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6781 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6841 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6901 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6961 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7021 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7081 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7141 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7201 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7261 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7321 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7381 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7441 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7501 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//