LOCUS       MR218237                9417 bp    DNA     circular  SYN 
DEFINITION  Eprs (Myc-DDK-tagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs)
ACCESSION   NM_029735
VERSION     NM_029735.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5574..5665 
                     /label="Myc-DDK"  
     misc_feature    5695..6280 
                     /label="PolyA signal"  
     misc_feature    5752..5771 
                     /label="Restriction Enzyme"  
     misc_feature    6556..7228 
                     /label="ColE1"  
     misc_feature    7658..8452 
                     /label="Kan/Neo"  
     misc_feature    8487..8789 
                     /label="SV40 ori"  
     misc_feature    8875..9352 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5556..5562 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5556 
                     /label="Eprs"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGCTG TGCCTCACGG TGAACGCCGG AAACCCTCCG CTGGAAGCTC 
     1081 TGCTGGCGGT GGAACATGTG AAAGGTGATG TCAGCATTTC TGTGGAAGAA GGGAAAGAGA 
     1141 ATCTTCTTCG GGTTTCTGAG ACTGTGGCAT TCACTGACGT GAATTCAATC CTGCGCTACC 
     1201 TGGCTAGAAT TGCAACTACG TCTGGGCTGT ATGGGACTAA CCTGATGGAG CACACTGAGA 
     1261 TTGATCATTG GTTGGAGTTT AGTGCTACAA AGTTGTCTTC GTGTGATAGG CTGACTTCTG 
     1321 CAATCAACGA GCTTAATCAT TGCCTGTCTC TGAGAACATA CCTAGTTGGG AACTCCTTGA 
     1381 CCTTAGCAGA TCTATGTGTT TGGGCCACCC TAAAAGGAAG TGCTGCATGG CAGGAACACT 
     1441 TGAAACAGAA CAAAACTCTA GTCCACGTTA AACGCTGGTT TGGCTTTCTT GAGGCCCAGC 
     1501 AGGCCTTCCG CTCAGTTGGC ACCAAGTGGG ATGTTTCAGG AAACAGAGCT ACAGTGGCAC 
     1561 CTGACAAAAA ACAAGATGTT GGGAAATTCG TGGAGCTTCC AGGTGCAGAG ATGGGAAAGG 
     1621 TGACAGTCAG ATTCCCCCCA GAGGCTAGTG GTTACTTACA CATTGGGCAT GCGAAAGCTG 
     1681 CTCTTCTGAA CCAGCACTAC CAGGTTAACT TTAAAGGGAA ACTGATCATG AGATTTGATG 
     1741 ACACAAACCC AGAAAAGGAA AAGGAAGATT TTGAGAAGGT CATCTTGGAA GATGTTGCAA 
     1801 TGCTGCATAT TAAACCAGAT CAGTTTACTT ACACTTCAGA TCATTTTGAA ACTATAATGA 
     1861 AGTATGCAGA GAAATTAATT CAAGAAGGGA AGGCTTATGT GGATGACACT CCTGCAGAAC 
     1921 AGATGAAGGC GGAACGTGAA CAGAGGACAG AATCCAAACA CAGGAAGAAC TCTGTTGAGA 
     1981 AGAATCTACA GATGTGGGAA GAAATGAAAA AAGGAAGCCA GTTTGGTCAG TCCTGTTGCC 
     2041 TGCGAGCAAA AATTGACATG AGTAGTAACA ATGGGTGTAT GAGAGACCCG ACCCTTTACC 
     2101 GATGCAAAAT CCAGCCCCAC CCAAGGACTG GGAATAAATA CAATGTTTAT CCAACATATG 
     2161 ACTTTGCCTG CCCTATAGTG GACAGCATTG AAGGTGTTAC TCATGCCTTG AGGACAACAG 
     2221 AGTACCACGA CCGAGATGAA CAATTTTATT GGATCATTGA AGCTTTAGGC ATAAGGAAAC 
     2281 CATACATCTG GGAGTACAGT CGCTTAAATC TCAACAACAC CGTGCTGTCC AAAAGAAAGC 
     2341 TCACGTGGTT TGTCAATGAA GGACTGGTAG ATGGATGGGA TGACCCACGG TTTCCTACAG 
     2401 TTCGTGGAGT TCTGAGAAGA GGGATGACAG TTGAGGGGCT GAAGCAGTTC ATTGCTGCTC 
     2461 AGGGCTCCTC AAGGTCTGTT GTGAACATGG AATGGGACAA AATCTGGGCG TTCAACAAAA 
     2521 AGGTTATTGA CCCAGTGGCT CCACGGTACG TTGCATTGCT GAAGAAGGAA GTCGTCCCAG 
     2581 TGAATGTCCT GGATGCGCAG GAGGAGATGA AGGAAGTAGC CAGACACCCC AAGAATCCTG 
     2641 ATGTTGGTTT GAAGCCTGTG TGGTACAGTC CCAAGGTTTT CATAGAAGGG GCCGATGCAG 
     2701 AGACTTTCTC AGAGGGTGAG ATGGTCACAT TTATAAACTG GGGCAACATC AACATTACTA 
     2761 AAATACACAA AAATGCAGAT GGAAAAATTA CATCTCTAGA TGCAAAATTG AATTTGGAAA 
     2821 ACAAAGACTA CAAGAAAACC ACCAAGATCA CTTGGCTTGC TGAGAGCACA CACGCTCTCT 
     2881 CGATCCCAGC CGTCTGTGTC ACTTATGAGC ACTTGATCAC AAAGCCAGTG CTGGGAAAAG 
     2941 ATGAGGACTT CAAGCAGTAC ATCAACAAAG ACAGTAAGCA TGAAGAGCTA ATGCTAGGAG 
     3001 ATCCTTGCCT TAAAGATCTG AAAAAAGGAG ACATCATACA ACTGCAGAGG AGAGGGTTCT 
     3061 TCATATGCGA CCAGCCATAC GAACCTGTCA GCCCATATAG TTGCAGAGAA GCTCCATGCA 
     3121 TCCTGATATA CATACCTGAT GGGCACACAA AGGAAATGCC AACATCCGGA TCAAAGGAAA 
     3181 AGACCAAAGT GGAAATCTCA AAAAAGGAGA CAAGCTCTGC TCCTAAAGAA AGACCAGCAC 
     3241 CTGCTGTGAG TTCTACTTGT GCTACAGCTG AGGATTCCTC CGTCCTCTAC AGCAGAGTGG 
     3301 CGGTGCAGGG GGATGTGGTT CGTGAATTGA AAGCCAAGAA AGCGCCAAAG GAAGATATAG 
     3361 ACGCAGCTGT GAAGCAGCTT TTGACTTTGA AAGCTGAATA TAAAGAGAAA ACTGGGCAGG 
     3421 AGTATAAACC TGGGAACCCC TCTGCTGCAG CAGTACAGAC TGTTTCTACA AAGTCATCAT 
     3481 CAAACACTGT GGAAAGTACA TCTTTATACA ATAAAGTCGC TGCCCAAGGG GAGGTGGTCC 
     3541 GAAAGCTGAA AGCGGAAAAG GCTCCTAAGG CTAAAGTAAC TGAAGCTGTG GAATGTTTGC 
     3601 TGTCCCTGAA AGCTGAGTAT AAAGAAAAGA CTGGGAAGGA CTATGTACCT GGCCAGCCCC 
     3661 CAGCATCGCA GAACTCACAT TCAAACCCTG TCAGTAATGC TCAGCCTGCT GGAGCGGAGA 
     3721 AACCAGAAGC CAAAGTGCTA TTTGACAGAG TGGCTTGTCA AGGAGAAGTA GTTCGAAAAC 
     3781 TTAAAGCTGA AAAGGCCTCT AAGGATCAAG TGGATTCGGC GGTACAAGAA CTTCTTCAGC 
     3841 TGAAGGCACA GTACAAGTCG CTGACAGGAA TCGAGTATAA GCCTGTGTCT GCTACTGGGG 
     3901 CAGAAGACAA AGATAAAAAG AAGAAAGAAA AAGAAAATAA ATCTGAAAAG CAGAACAAGC 
     3961 CTCAGAAACA AAATGATGGC CAAGGGAAAG ACTCTTCCAA AAGCCAAGGC AGTGGCCTGT 
     4021 CATCAGGTGG AGCAGGAGAA GGGCAGGGGC CTAAGAAACA GACCAGATTG GGTCTTGAAG 
     4081 CAAAAAAAGA AGAAAATCTT GCCGAATGGT ACTCTCAAGT CATCACAAAG TCCGAGATGA 
     4141 TTGAATACTA CGATGTGAGT GGCTGCTATA TCCTCCGTCC CTGGTCGTAC TCCATCTGGG 
     4201 AGTCCATTAA GGACTTCTTT GACGCTGAGA TCAAGAAGCT CGGTGTTGAG AACTGCTACT 
     4261 TCCCCATATT TGTGTCTCAG GCTGCGTTAG AGAAAGAGAA GAATCACATC GAAGACTTTG 
     4321 CTCCTGAGGT TGCTTGGGTT ACAAGATCAG GAAAAACAGA GTTGGCAGAG CCAATTGCCA 
     4381 TTCGTCCTAC TAGTGAAACA GTGATGTATC CGGCATATGC TAAATGGGTG CAGTCCCACA 
     4441 GAGACCTGCC CGTCAGGCTC AACCAGTGGT GCAATGTGGT GCGCTGGGAG TTCAAACACC 
     4501 CACAGCCTTT CCTCCGAACT CGGGAGTTCC TGTGGCAGGA GGGGCACAGC GCCTTTGCCA 
     4561 CCTTTGAGGA AGCAGCTGAT GAGGTTTTAC AGATCCTTGA GTTATATGCT CGAGTATATG 
     4621 AAGAGCTCTT AGCCATTCCT GTTGTGAGAG GAAGGAAGAC TGAAAAGGAG AAGTTTGCAG 
     4681 GAGGAGACTA CACAACCACA ATAGAAGCCT TCATATCTGC CAGCGGACGC GCCATTCAGG 
     4741 GAGCAACATC ACACCATTTA GGCCAGAATT TCTCCAAAAT GTGTGAAATA GTTTTTGAAG 
     4801 ATCCCAAGAC ACCAGGAGAA AAGCAGTTTG CATACCAGTG CTCCTGGGGC CTGACAACAC 
     4861 GAACCATCGG TGTGATGGTC ATGGTCCATG GGGACAACAT GGGCCTAGTG CTTCCACCCC 
     4921 GAGTAGCATC TGTCCAGGTG GTAGTCATTC CCTGTGGAAT CACAAATGCC CTGTCTGAAG 
     4981 AAGACAGAGA GGCACTGATG GCAAAATGCA ACGAGTACAG AAGGCGGCTG CTTGGTGCTA 
     5041 ACATCCGAGT CCGAGTCGAT CTACGAGACA ACTACTCCCC AGGCTGGAAG TTCAACCACT 
     5101 GGGAACTCAA GGGAGTTCCA GTCAGACTTG AGGTGGGGCC ACGTGATATG AAGAGCTGTC 
     5161 AGTTTGTAGC AGTCAGACGA GATACTGGAG AGAAACTGAC AATTGCTGAG AAGGAGGCTG 
     5221 AGGCTAAGCT GGAAAAGGTT TTGGAAGACA TCCAGCTGAA CCTTTTCACA AGGGCTTCTG 
     5281 AAGACCTTAA AACCCACATG GTTGTGTCTA ATACACTGGA GGACTTCCAG AAGGTGCTAG 
     5341 ATGCTGGAAA GGTTGCACAG ATTCCATTTT GTGGGGAAAT TGACTGTGAG GACTGGATCA 
     5401 AAAAGATGAC TGCCAGGGAT CAAGATGTGG AACCTGGTGC TCCATCCATG GGAGCCAAAA 
     5461 GCCTTTGCAT TCCTTTCAAC CCTCTGTGTG AGCTGCAGCC CGGAGCCATG TGTGTCTGTG 
     5521 GCAAGAATCC TGCCAAGTTC TACACCTTGT TTGGTCGGAG TTACACGCGT ACGCGGCCGC 
     5581 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5641 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5701 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5761 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5821 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5881 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5941 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     6001 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     6061 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6121 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6181 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6241 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6301 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6361 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6421 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6481 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6541 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6601 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6661 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6721 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6781 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6841 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6901 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6961 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     7021 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7081 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7141 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7201 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7261 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7321 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7381 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7441 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7501 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7561 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7621 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7681 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7741 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7801 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7861 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7921 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7981 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     8041 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     8101 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8161 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8221 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8281 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8341 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8401 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8461 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8521 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8581 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8641 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8701 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8761 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8821 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8881 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8941 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     9001 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     9061 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9121 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9181 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9241 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9301 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9361 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//