LOCUS       MR218131                8811 bp    DNA     circular  SYN 
DEFINITION  Gpr125 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 125 (Gpr125)
ACCESSION   NM_133911
VERSION     NM_133911.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4968..5059 
                     /label="Myc-DDK"  
     misc_feature    5089..5674 
                     /label="PolyA signal"  
     misc_feature    5146..5165 
                     /label="Restriction Enzyme"  
     misc_feature    5950..6622 
                     /label="ColE1"  
     misc_feature    7052..7846 
                     /label="Kan/Neo"  
     misc_feature    7881..8183 
                     /label="SV40 ori"  
     misc_feature    8269..8746 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4950..4956 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4950 
                     /label="Adgra3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCCG CCGCCGCTGC TGCTGCTGCC GCTGGCGCTG CTCGCTCTGC 
     1081 TGTGGGGAGG AGAGCGCGGG GCCGCGGCGC TGCCCGCGGG CTGCAAGCAC GACGGTCGGG 
     1141 CCCGGGGTAC CGGCCGAGCG GCCGCCGCCG CCGAGGGGAA GGTGGTGTGC AGCAGCCTGG 
     1201 AGCTCGCGCA GGTTCTGCCC CCGGACACGC TGCCCAACCG CACGGTCACC CTGATTTTAA 
     1261 GCAACAACAA GATCTCCGAG CTGAAGAATG GTTCATTTTC TGGCTTAAGT CTCCTCGAAA 
     1321 GACTGGACCT CCGGAACAAC CTTATTAGTA GGATAGCCCC AGGTGCCTTT TGGGGACTGT 
     1381 CTTCACTGAA GAGATTGGAC CTGACGAACA ACCGAATAGG TTGTCTGAAT GCAGATGTAT 
     1441 TTCGAGGACT CACCAATCTG GTTCGGCTAA ACCTTTCAGG GAATCTGTTT ACTTCACTGT 
     1501 CTCAAGGAAC TTTTGATTAT CTTGGCTCGT TGCGGTCTTT AGAATTTCAG ACTGAGTACC 
     1561 TTCTGTGTGA CTGTAACATT CTGTGGATGC ATCGCTGGGT AAAGGAGAGA AACATCACTG 
     1621 TGCGAGACAC CAGGTGTGTT TATCCCAAGT CACTGCAGGC CCAGCCTGTC ACGGGGGTGA 
     1681 AACAGGAGCT CCTCACTTGC GATCCTCCCC TTGAACTGCC GTCCTTCTAC ATGACTCCGT 
     1741 CGCACCGCCA GGTTGTGTTT GAAGGAGACA GCCTTCCCTT CCAGTGCATG GCTTCCTATA 
     1801 TAGATCAGGA CATGCAAGTG CTGTGGTATC AGGATGGGCG CATTGTTGAG ACCGATGAGT 
     1861 CCCAAGGGAT CTTTGTGGAG AAAAGCATGA TTCACAATTG CTCCTTGATC GCCAGTGCCC 
     1921 TAACCATTTC TAATATTCAA GCTGGATCTA CTGGAAATTG GGGCTGTCAT GTTCAGACGA 
     1981 AACGTGGGAA TAACACAAGA ACTGTTGACA TTGTGGTATT AGAAAGCTCC GCCCAATACT 
     2041 GTCCACCAGA GAGGGTTGTG AACAACAAAG GTGATTTCAG ATGGCCCAGG ACACTGGCGG 
     2101 GCATCACAGC ATATCTCCAG TGTACCCGGA ACACCCACAG CAGTGGGATC TACCCTGGAA 
     2161 GCGCACAGGA TGAAAGGAAG GCGTGGCGCC GATGCGACAG AGGTGGCTTT TGGGCAGATG 
     2221 ATGATTATTC TAGGTGCCAG TATGCAAATG ACGTCACTAG AGTCCTGTAT ATGTTTAATC 
     2281 AGATGCCCCT CAACCTTACA AATGCGGTCG CTACAGCTCG GCAGCTGCTG GCTTACACAG 
     2341 TGGAGGCCGC CAACTTCTCT GACAAAATGG ACGTTATATT TGTGGCTGAA ATGATAGAAA 
     2401 AGTTTGGAAG ATTTACCAGA GAGGAAAAAT CAAAAGAGCT TGGTGATGTA ATGGTCGATG 
     2461 TGGCAAGCAA CATCATGTTG GCTGATGAGC GGGTCCTGTG GCTGGCACAG AGGGAAGCAA 
     2521 AGGCCTGCAG TCGGATTGTC CAGTGCCTGC AGCGCATTGC CACACATCGC CTGGCCAGTG 
     2581 GGGCCCACGT GTACTCCACG TACTCGCCCA ACATTGCTCT GGAGGCTTAC GTCATCAAGG 
     2641 CTGCTGGCTT CACAGGAATG ACCTGCTCCG TGTTCCAGAA GGTGGCTGCC TCCGACCGTG 
     2701 CAGGTCTTTC TGACTATGGG CGAAGGGACC CGGATGGAAA CCTGGATAAG CAGCTGAGCT 
     2761 TCAAATGCAA TGTCTCCAGC ACCTTCTCAA GCCTGGCCCT GAAGAACACC ATCATGGAGG 
     2821 CCTCCATTCA GCTTCCTTCC TCCCTTTTGT CACCAAAACA CAAGCGAGAA GCCCGAGCGG 
     2881 CGGATGACGC CCTCTATAAG CTCCAGCTCA TTGCCTTCCG CAACGGAAAG CTTTTTCCAG 
     2941 CCACAGGAAA TTCAACAAAG TTGGCAGACG ATGGCAAGCG GCGGACAGTA GTGACCCCTG 
     3001 TGATCCTCAC GAAAATAGAT GGTGCAACCG TAGATACCCA CCACATCCCT GTTAATGTGA 
     3061 CGCTGCGCCG AATTGCCCAC GGAGCAGATG CGGTTGCAGC GCAGTGGGAC TTTGATTTGC 
     3121 TGAACGGACA AGGAGGCTGG AAGTCAGATG GGTGCTGTAT ACTCTACTCG GATGAGAACA 
     3181 TCACCACCAT TCAGTGCGGC TCCCTGGGCA ACTATGCTGT GCTAATGGAT CTGACTGGGA 
     3241 CAGAGTTGTA CACCCCAGCA GCCAGTCTCC TGCACCCTGT GGTTTACACC ACTGCCATCA 
     3301 CTCTCCTCTT GTGTCTCTTG GCTGTTATCA TCAGTTACAT GTACCACCAC AGCTTGATCC 
     3361 GAATCAGTCT CAAGAGCTGG CACATGCTTG TGAACCTGTG CTTTCACATT CTCCTGACCT 
     3421 GCGTGGTGTT TGTGGGAGGA ATAACCCAGA CCAGAAATGC CAGCGTCTGT CAAGCAGTTG 
     3481 GGATCATTCT TCATTATTCT ACCCTTGCCA CGGTATTGTG GGTTGGAGTC ACTGCTAGAA 
     3541 ATATCTATAA ACAAGTCACC AAGAAAGCCA AGAGATGCCA GGATCCAGAT GAGCCACCCG 
     3601 CTCCTCCACG ACCGATGCTG AGGTTCTACC TGATTGGTGG TGGGATCCCC ATCATAGTGT 
     3661 GTGGTATCAC CGCGGCAGCA AACATCAAGA ACTATGGCAG TCGGCCCAGT GCACCGTATT 
     3721 GCTGGATGGC CTGGGAACCG TCCTTGGGAG CCTTCTACGG ACCTGCCAGC TTCATCACTT 
     3781 TTGTAAACTG TATGTATTTT CTAAGCATAT TTATTCAGTT GAAAAGACAC CCTGAGCGCA 
     3841 AATATGAGCT CAAGGAGCCG ACAGAAGAGC AGCAGAGATT GGCAGCCAAT GAAAATGGTG 
     3901 AAATCAACCA TCAGGACTCC ATGTCTCTGT CTCTCATCTC TACGTCCACG TTGGAGAACG 
     3961 AGCACAGTTT TCAGTCTCAG CTTCTGGGCG CCAGCCTTAC TTTGCTTTTG TATGTCATCT 
     4021 TGTGGATGTT TGGGGCCATG GCTGTTTCTC TGTATTACCC TCTGGACTTG GTTTTTAGCT 
     4081 TCTTCTTCGG AGCCACTTGT TTAAGCTTCA GTGCTTTCAT GATGGTGCAC CACTGCATCA 
     4141 ACAGGGAGGA CGTGAGACTT GCGTGGATCA TGATGTGCTG CCCAGGGCGG AGCTCGTACT 
     4201 CCGTGCAAGT CAACGTCCAA CCTCCCAACT CAAGCGCCAC TAATGGAGAG GCTCCAAAGT 
     4261 GCACCAATAG CAGCGCAGAG TCTTCGTGCA CGAACAAAAG CGCATCGAGC TTCAAAAACT 
     4321 CTTCCCAGGG CTGCAAGCTG ACAAACTTGC AGGCTGCTGC GGCACAGTAC CACAGCAATG 
     4381 CCCTACCTGT GAATGCCACG CCGCAGCTTG ATAACAGTCT GACCGAACAC TCGATGGACA 
     4441 ACGATATTAA AATGCATGTG GCTCCTTTAG ACGTGCAGTT TCGAACAAAC GTGCACCCAA 
     4501 GCCGCCACCA CAAAAACCGA AGTAAAGGAC ACCGGGCCAG CAGGCTCACA GTCCTGCGAG 
     4561 AGTATGCCTA TGACGTCCCA ACAAGTGTGG AAGGAAGCGT GCAGAATGGC TTACCTAAAA 
     4621 GCCGGCCAGG CAGCAATGAA GGACATTCAA GGAGTAGGAG AGCTTATTTA GCCTACAGAG 
     4681 AGAGACAGTA CAACCCACCC CAACAAGACA GCAGTGATGC TTGTAGCACA CTTCCCAAAA 
     4741 GTAGCCGAAA TGTTGAAAAG CCTGTTTCAA CTAGTAGTAA GAAAGATGCA CCAAGGAAGC 
     4801 CAGCTGCAGC CGACCTTGAA AGTCAGCAGA AATCTTACGG CCTGAACTTG GCTGTTCAGA 
     4861 ATGGACCAGT TAAAAGCAAT GGGCAGGAAG GACCCTTGCT AGCTACCGAC GTCACTGGTA 
     4921 ATGTTAGGAC TGGGTTATGG AAACACGAAA CAACTGTGAC GCGTACGCGG CCGCTCGAGC 
     4981 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5041 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5101 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5161 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5221 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5281 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5341 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5401 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5461 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5521 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5581 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5641 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5701 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5761 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5821 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5881 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5941 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6001 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6061 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6121 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6181 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6241 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6301 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6361 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6421 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6481 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6541 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6601 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6661 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6721 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6781 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6841 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6901 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6961 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7021 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7081 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7141 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7201 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7261 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7321 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7381 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7441 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7501 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7561 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7621 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7681 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7741 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7801 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7861 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7921 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7981 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8041 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8101 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8161 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8221 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8281 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8341 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8401 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8461 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8521 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8581 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8641 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8701 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8761 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//