LOCUS       MR218117                8166 bp    DNA     circular  SYN 
DEFINITION  Msh3 (Myc-DDK-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3)
ACCESSION   NM_010829
VERSION     NM_010829.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4323..4414 
                     /label="Myc-DDK"  
     misc_feature    4444..5029 
                     /label="PolyA signal"  
     misc_feature    4501..4520 
                     /label="Restriction Enzyme"  
     misc_feature    5305..5977 
                     /label="ColE1"  
     misc_feature    6407..7201 
                     /label="Kan/Neo"  
     misc_feature    7236..7538 
                     /label="SV40 ori"  
     misc_feature    7624..8101 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4305..4311 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4305 
                     /label="Msh3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCGGG AAATCCGCGT CGGGAGGCTC TACTGCCGCC GGCCCAGGCC 
     1081 CGGGGAGGCA AACGGTTCTA AGCCGGTTCT TCAGGTCTGC GGGAAGCCTA AGATCCAGCG 
     1141 CGTCCTCCAC GGAGCCAGCA GAGAAGGTGA CAGAAGGTGA CAGCAGGAAG AGGTCACTGG 
     1201 GAAATGGTGG GCCCACTAAG AAAAAAGCAA GGAAAGTTCC AGAGAAGGAG GAAGAAAACA 
     1261 TCTCAGTAGC AGCTCACCAC CCTGAGGCAA AGAAATGTCT GAGGCCCAGG ATTGTTTTAA 
     1321 AGTCCCTGGA AAAGTTGAAA GAATTCTGCT GTGATTCTGC CCTCCCTCAA AACAGAGTCC 
     1381 AGACAGAAGC TCTTCGGGAG AGACTTGAAG TTCTGCCAAG GTGTACTGAT TTTGAAGATA 
     1441 TCACTCTGCA ACGTGCAAAG AATGCGGTTT TGTCCGAAGA TTCCAAATCT CAGGCTAATC 
     1501 AGAAAGACAG TCAATTTGGA CCCTGCCCTG AAGTTTTCCA GAAGACTTCC GATTGTAAAC 
     1561 CTTTTAACAA GCGATCCAAG AGCGTCTATA CACCGTTAGA ACTGCAGTAC TTAGATATGA 
     1621 AGCAGCAGCA TAAAGACGCA GTTTTGTGTG TGGAGTGTGG TTACAAGTAC AGATTCTTTG 
     1681 GGGAAGATGC AGAAATTGCT GCCCGGGAAC TCAATATTTA TTGCCACTTA GACCACAACT 
     1741 TTATGACTGC AAGCATACCT ACCCACAGAC TGTTCGTGCA TGTCCGCCGA CTTGTGGCCA 
     1801 AAGGGTACAA GGTGGGAGTT GTGAAGCAAA CTGAAACTGC CGCATTAAAG GCCATCGGAG 
     1861 ACAATAAAAG TTCTGTCTTC TCCCGGAAAT TGACCGCTCT TTATACGAAA TCCACACTTA 
     1921 TTGGTGAAGA TGTGAATCCT CTCATCAGGC TGGATGATTC TGTAAATATC GATGAGGTAA 
     1981 TGACAGATAC ATCTACCAAC TATCTTCTGT GTATCTACGA AGAAAAGGAG AACATTAAAG 
     2041 ACAAAAAGAA GGGGAACCTT TCCGTTGGTA TCGTGGGAGT GCAGCCCGCA ACTGGCGAGG 
     2101 TTGTGTTTGA CTGCTTCCAG GACTCTGCTT CCAGACTGGA GCTGGAGACT CGCATATCAA 
     2161 GCCTGCAGCC TGTGGAATTA CTGCTTCCCT CCGACCTGTC TGTGCCAACA GAGATGCTCA 
     2221 TCCAAAGAGC CACCAATGTC AGTGTGCGGG ATGACAGGAT TCGAGTAGAA AGGATGAATA 
     2281 ATACTTACTT TGAATACAGC CATGCTTTCC AGACAGTTAC GGAGTTTTAT GCTAGAGAGA 
     2341 TAGTTGACAG CCAGGGCTCT CAGAGTCTCT CTGGTGTCAT TAACTTGGAG AAGCCTGTGA 
     2401 TCTGCGCCTT GGCTGCCGTA ATAAGGTACC TCAAGGAATT TAACCTGGAA AAGATGCTGT 
     2461 CCAAACCCGA GAGTTTTAAA CAGCTGTCAA GTGGAATGGA ATTCATGAGA ATTAATGGGA 
     2521 CCACGCTAAG GAATCTGGAG ATCCTTCAGA ATCAGACTGA TATGAAGACC AAAGGAAGCT 
     2581 TGCTTTGGGT TTTAGACCAC ACTAAAACTT CATTTGGGAG AAGGAAGTTA AAGAATTGGG 
     2641 TTACCCAGCC ACTGCTTAAA TTAAGGGAAA TAAATGCCCG ACTTGATGCA GTATCTGATG 
     2701 TTCTCCACTC AGAATCCAGT GTGTTTGAGC AGATAGAAAA CCTTCTTCGT AAGTTACCGG 
     2761 ATGTCGAGAG AGGACTATGT AGCATTTATC ACAAGAAGTG CTCTACCCAA GAGTTCTTCT 
     2821 TGATCGTCAA AAGCCTATGT CAACTGAAGT CAGAGCTTCA AGCATTAATG CCTGCTGTTA 
     2881 ATTCCCACGT TCAGTCCGAC TTACTCCGAG CACTCATCGT GGAGGCTCCT GAGCTCCTCA 
     2941 GCCCAGTGGA ACATTACTTA AAGGTTCTGA ATGGGCCAGC TGCCAAAGTT GGCGATAAAA 
     3001 CTGAATTATT CAAAGACCTC TCTGACTTCC CTTTAATAAA AAAGAGGAAA AATGAAATTC 
     3061 AGGAAGTCAT TCACAGCATC CAAATGCGTT TACAGGAATT TCGGAAAATA TTAAAACTTC 
     3121 CTTCTTTACA GTATGTGACT GTATCAGGAC AAGAGTTTAT GATTGAAATA AAGAACTCGG 
     3181 CTGTATCTTG CATCCCAGCT GATTGGGTGA AGGTTGGAAG CACAAAAGCT GTGAGCCGCT 
     3241 TTCACCCTCC CTTCATCGTG GAAAGCTACA GACGTCTGAA TCAGCTCCGG GAGCAGCTAG 
     3301 TCCTTGACTG CAATGCTGAA TGGCTTGGCT TCCTAGAGAA TTTTGGTGAA CATTATCACA 
     3361 CTTTGTGTAA GGCTGTGGAT CACCTAGCAA CTGTTGACTG TATTTTCTCC CTGGCCAAGG 
     3421 TCGCTAAACA AGGAAATTAC TGCAGGCCAA CTCTACAAGA AGAAAAGAAG ATCATCATAA 
     3481 AAAATGGAAG GCACCCTATG ATTGATGTGT TGCTGGGTGA ACAAGATCAG TTTGTGCCCA 
     3541 ACAGTACGAG TTTATCACAG GACTCGGAGA GAGTAATGAT AATCACCGGA CCAAACATGG 
     3601 GTGGGAAAAG CTCCTACATA AAACAGGTTG CACTGGTTAC CATCATGGCT CAGATTGGCT 
     3661 CCTACGTCCC TGCAGAGGAA GCCACAATTG GGATTGTGGA TGGCATTTTC ACAAGGATGG 
     3721 GTGCTGCAGA CAATATATAC AAAGGCCGGA GTACTTTCAT GGAAGAACTG ACGGACACAG 
     3781 CGGAAATAAT CAGGAGAGCG TCCCCACAGT CCTTGGTTAT CCTGGATGAG CTGGGGAGAG 
     3841 GGACAAGCAC CCATGACGGA ATCGCCATCG CCTATGCAAC TCTAGAGTAT TTTATCAGAG 
     3901 ACGTGAAATC CTTAACACTG TTCGTTACCC ACTATCCACC AGTCTGTGAA CTAGAAAAAT 
     3961 GTTACCCAGA ACAAGTGGGG AATTACCACA TGGGATTCTT GGTCAATGAA GATGAAAGCA 
     4021 AGCAGGACTC AGGTGACATG GAGCAGATGC CGGATTCTGT CACTTTTCTC TATCAGATAA 
     4081 CCCGAGGAAT TGCAGCGAGA AGTTATGGAC TTAATGTGGC GAAGTTAGCA GATGTACCTA 
     4141 GAGAAGTCTT ACAGAAAGCC GCGCACAAGT CTAAGGAGCT GGAAGGCTTA GTCAGCCTGA 
     4201 GAAGGAAAAG GCTTGAATGT TTTACCGACT TATGGACGAC ACACAGTGTG AAGGACCTGC 
     4261 ACACATGGGC AGATAAGCTG GAAATGGAGG AAATACAGAC TTCTCTCCCA CATACGCGTA 
     4321 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4381 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4441 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4501 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4561 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4621 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4681 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4741 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4801 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4861 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4921 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4981 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5041 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5101 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5161 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5221 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5281 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5341 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5401 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5461 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5521 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5581 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5641 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5701 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5761 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5821 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5881 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5941 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6001 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6061 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6121 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6181 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6241 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6301 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6361 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6421 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6481 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6541 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6601 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6661 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6721 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6781 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6841 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6901 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6961 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7021 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7081 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7141 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7201 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7261 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7321 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7381 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7441 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7501 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7561 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7621 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7681 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7741 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7801 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7861 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7921 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7981 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8041 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8101 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8161 AATTTT
//