LOCUS       MR218114                9231 bp    DNA     circular  SYN 
DEFINITION  Cpsf1 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 1 (Cpsf1), transcript variant 1
ACCESSION   NM_001164173
VERSION     NM_001164173.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5388..5479 
                     /label="Myc-DDK"  
     misc_feature    5509..6094 
                     /label="PolyA signal"  
     misc_feature    5566..5585 
                     /label="Restriction Enzyme"  
     misc_feature    6370..7042 
                     /label="ColE1"  
     misc_feature    7472..8266 
                     /label="Kan/Neo"  
     misc_feature    8301..8603 
                     /label="SV40 ori"  
     misc_feature    8689..9166 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5370..5376 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5370 
                     /label="Cpsf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACGCGGTG TACAAGCAGG CGCACCCGCC CACCGGGCTG GAGTTTACCA 
     1081 TGTACTGCAA CTTCTTCAAC AACAGTGAGC GCAACCTCGT GGTGGCCGGC ACCTCGCAGC 
     1141 TCTATGTGTA CCGCCTGAAC CGCGACGCCG AGGCTCTGAC CAAGAATGAT GGGAGCACAG 
     1201 AGGGGAAGGC CCACCGAGAG AAACTAGAGC TGGTAGCCTC CTTCTCTTTT TTTGGCAATG 
     1261 TCATGTCCAT GGCCAGTGTG CAGCTTGCAG GCGCCAAACG TGATGCCTTG CTTCTCAGTT 
     1321 TCAAAGACGC TAAGCTGTCT GTGGTGGAGT ATGACCCAGG CACTCATGAC CTGAAGACCC 
     1381 TGTCTCTACA CTACTTTGAG GAGCCTGAAC TTCGGGATGG GTTTGTGCAG AACGTGCATA 
     1441 CGCCTCGTGT GCGGGTGGAC CCTGACGGGC GCTGTGCAGC AATGCTCATC TATGGCACGA 
     1501 GGCTGGTAGT TCTGCCTTTC CGCAGAGAGA GCCTGGCTGA GGAACATGAG GGGCTCATGG 
     1561 GCGAAGGGCA GAGGTCTAGC TTCTTGCCCA GCTACATCAT TGATGTACGG GCTCTGGATG 
     1621 AGAAACTACT CAACATCATT GACCTGCAGT TCCTGCATGG CTACTATGAG CCCACCCTGC 
     1681 TTATCCTGTT TGAGCCCAAC CAGACTTGGC CAGGGCGGGT GGCTGTGAGG CAGGACACGT 
     1741 GCTCCATTGT GGCTATCTCG CTGAACATCA CACAGAAAGT CCATCCAGTC ATCTGGTCCC 
     1801 TCACCAGCTT GCCTTTTGAC TGTACCCAGG CCCTGGCTGT GCCCAAGCCC ATAGGTGGGG 
     1861 TAGTGATCTT CGCTGTCAAC TCACTGTTGT ACCTGAACCA GAGTGTTCCC CCATATGGTG 
     1921 TGGCTCTCAA TAGTCTCACC ACAGGCACCA CCGCTTTCCC ATTGCGTACC CAAGAGGGTG 
     1981 TACGGATCAC CCTAGACTGC GCACAGGCGG CCTTCATCTC TTATGACAAG ATGGTCATCT 
     2041 CCCTCAAGGG TGGTGAGATT TATGTGCTGA CCCTCATCAC TGACGGCATG CGAAGTGTCC 
     2101 GAGCGTTTCA CTTTGACAAG GCAGCTGCTA GTGTCCTTAC CACCAGTATG GTCACAATGG 
     2161 AGCCCGGATA CCTGTTCCTA GGCTCTCGCC TGGGCAATTC CCTCCTCCTC AAGTACACGG 
     2221 AGAAGCTGCA GGAGCCCCCA GCCAGCTCCG TCCGTGAGGC TGCTGACAAG GAAGAGCCAC 
     2281 CCTCAAAGAA GAAGCGAGTG GAGCCTGCAG TGGGCTGGAC AGGAGGCAAG ACGGTGCCAC 
     2341 AGGACGAGGT GGATGAAATT GAAGTTTATG GCAGTGAGGC CCAGTCAGGC ACACAGCTGG 
     2401 CTACTTACTC TTTTGAGGTA TGTGACAGCA TGCTCAACAT TGGACCCTGT GCCAACGCTG 
     2461 CTGTGGGCGA GCCTGCCTTC CTCTCTGAAG AGTTCCAGAA TAGCCCTGAG CCAGACCTGG 
     2521 AGATTGTGGT TTGTTCCGGC TACGGGAAGA ATGGGGCCCT GTCAGTGTTG CAGAAGAGTA 
     2581 TCCGGCCCCA GGTGGTGACA ACCTTTGAAC TTCCTGGCTG CTATGATATG TGGACAGTCA 
     2641 TTGCTCCTGT GCGGAAAGAG GAGGAAGAAA CCCCCAAAGC AGAGAGCACA GAGCAGGAGC 
     2701 CCAGTGCCCC GAAAGCAGAG GAGGATGGGC GAAGACATGG CTTCCTTATT CTTAGCCGGG 
     2761 AAGACTCAAC CATGATCCTA CAGACTGGCC AGGAGATCAT GGAGCTGGAC ACCAGTGGTT 
     2821 TTGCCACTCA GGGTCCTACT GTCTTTGCTG GAAACATTGG GGACAATCGT TACATTGTTC 
     2881 AAGTCTCGCC ACTGGGCATT CGCCTGTTGG AAGGCGTGAA TCAATTGCAC TTCATCCCTG 
     2941 TGGACCTGGG CGCCCCCATC GTGCAGTGTG CCGTGGCTGA TCCCTATGTG GTTATCATGA 
     3001 GTGCTGAGGG ACACGTTACC ATGTTCCTAC TCAAGAGCGA TTCCTATGGT GGCCGTCATC 
     3061 ACCGCCTGGC ACTACACAAG CCTCCACTGC ATCATCAGTC CAAGGTGATT GCTCTCTGCC 
     3121 TGTACCGCGA TGTCAGTGGC ATGTTCACCA CTGAGAGCCG CCTGGGTGGG GCCCGTGATG 
     3181 AACTGGGTGG CCGAAGTGGC TCCGAGGCAG AAGGCCTGGG CTCAGAAACG AGCCCCACAG 
     3241 TGGATGATGA GGAAGAGATG CTCTATGGGG ACTCCAGTGC CCTCTTCAGT CCCAGCAAAG 
     3301 AGGAGGCCCG TAGGAGCAGC CAGCCACCGG CTGATCGTGA CCCTGCACCC TTCAAGGCAG 
     3361 ACCCCACTCA CTGGTGCCTG CTGGTGCGGG AGAATGGCAC CATGGAGATC TACCAGCTCC 
     3421 CAGACTGGCG GCTGGTATTC CTAGTTAAGA ACTTCCCCGT GGGGCAGCGG GTTCTGGTGG 
     3481 ACAGTTCATT TGGACAGCCC ACTACACAAG GTGAAGTCCG AAAGGAAGAA GCCACACGCC 
     3541 AGGGGGAGCT GCCTTTGGTT AAGGAGGTGT TGTTGGTTGC TCTGGGAAGC CGGCAGAGCA 
     3601 GGCCCTACCT GCTGGTACAC GTGGACCAGG AGTTGCTTAT CTATGAAGCC TTTCCCCATG 
     3661 ACTCTCAGCT TGGCCAGGGA AATCTTAAAG TCCGTTTCAA AAAGGTCCCT CACAATATCA 
     3721 ACTTCCGTGA AAAGAAGCCA AAGCCGTCCA AGAAGAAGGC CGAAGGTTGC AGCACTGAGG 
     3781 AGGGATCTGG AGGCCGGGGC CGTGTGGCAC GTTTCCGCTA CTTTGAGGAT ATTTATGGCT 
     3841 ATTCGGGGGT TTTCATCTGT GGACCCTCAC CTCACTGGCT CCTGGTGACT GGCCGCGGAG 
     3901 CTCTGCGTCT GCACCCCATG GGCATTGATG GCCCCATTGA CTCCTTTGCT CCTTTCCACA 
     3961 ATGTGAACTG CCCCCGTGGT TTCCTGTACT TCAACAGACA GGGGGAGCTG AGGATCAGCG 
     4021 TCCTGCCTGC CTACCTGTCC TACGATGCCC CATGGCCTGT CAGGAAGATC CCACTCCGAT 
     4081 GCACAGCCCA CTATGTGGCC TACCATGTGG AATCCAAGGT CTATGCTGTG GCCACTAGTA 
     4141 CCAACACACC TTGCACTAGG ATCCCACGCA TGACTGGCGA GGAAAAAGAA TTCGAGGCCA 
     4201 TTGAGAGAGA CGACAGATAC ATCCACCCCC AGCAAGAAGC CTTCTCCATC CAGCTCATCT 
     4261 CCCCGGTCAG CTGGGAGGCC ATTCCCAATG CCAGGATCGA GCTAGAGGAA TGGGAGCATG 
     4321 TCACGTGCAT GAAGACAGTG TCACTGCGCA GTGAGGAGAC CGTGTCAGGC CTAAAGGGCT 
     4381 ATGTGGCTGC TGGGACCTGC CTCATGCAGG GGGAGGAGGT CACGTGCCGT GGGCGGATCC 
     4441 TGATCATGGA TGTGATTGAA GTGGTGCCTG AGCCTGGGCA GCCCCTGACC AAGAACAAAT 
     4501 TCAAGGTCCT GTATGAGAAG GAGCAGAAAG GGCCCGTGAC TGCGCTGTGC CACTGCAATG 
     4561 GCCACCTGGT GTCGGCCATT GGCCAAAAAA TCTTCCTGTG GAGCCTGCGG GCTAGCGAGC 
     4621 TGACCGGCAT GGCCTTCATC GACACCCAGC TGTACATCCA CCAGATGATC AGTGTCAAGA 
     4681 ACTTCATCCT GGCAGCAGAC GTCATGAAGA GCATCTCACT GCTGCGTTAC CAAGAGGAGA 
     4741 GCAAGACGCT GAGCCTGGTG TCCCGGGATG CCAAGCCTTT GGAGGTATAC AGCGTGGACT 
     4801 TCATGGTGGA CAATGCCCAA CTGGGCTTCT TGGTGTCTGA CCGAGACCGC AATCTTATGG 
     4861 TGTATATGTA TCTGCCAGAA GCCAAAGAGA GTTTTGGGGG CATGCGCCTG CTACGCAGAG 
     4921 CGGATTTCCA TGTGGGTGCC CACGTGAATA CGTTCTGGAG GACCCCGTGC CGAGGTGCTG 
     4981 CCGAGGGCCC CAGCAAGAAG TCAGTCGTGT GGGAAAACAA GCATATCACA TGGTTTGCAA 
     5041 CCCTTGACGG TGGCATTGGG CTCCTGCTGC CCATGCAAGA AAAGACATAC CGACGCTTGC 
     5101 TGATGCTGCA GAATGCACTG ACGACCATGC TACCCCACCA CGCTGGCCTC AACCCCCGCG 
     5161 CCTTCCGAAT GTTGCACGTA GACCGGCGCA TCTTGCAGAA TGCTGTGCGC AACGTCCTTG 
     5221 ATGGGGAACT GCTAAACCGC TACCTATACC TCAGCACCAT GGAGCGCAGT GAGCTGGCCA 
     5281 AGAAGATAGG TACCACGCCT GACATTACCG GGTCACGGCT CACTTCTAAC TCTAGGATGC 
     5341 TGCTCTGGTG TTGTACACTT CCCTCTACCC ATTTTGTAAC GCGTACGCGG CCGCTCGAGC 
     5401 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5461 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5521 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5581 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5641 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5701 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5761 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5821 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5881 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5941 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6001 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6061 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6121 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6181 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6241 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6301 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6361 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6421 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6481 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6541 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6601 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6661 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6721 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6781 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6841 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6901 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6961 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7021 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7081 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7141 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7201 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7261 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7321 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7381 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7441 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7501 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7561 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7621 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7681 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7741 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7801 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7861 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7921 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7981 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8041 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8101 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8161 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8221 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8281 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8341 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8401 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8461 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8521 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8581 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8641 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8701 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8761 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8821 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8881 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8941 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9001 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9061 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9121 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9181 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//