LOCUS       MR218018                8394 bp    DNA     circular  SYN 
DEFINITION  Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3)
ACCESSION   NM_019670
VERSION     NM_019670.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4551..4642 
                     /label="Myc-DDK"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    4729..4748 
                     /label="Restriction Enzyme"  
     misc_feature    5533..6205 
                     /label="ColE1"  
     misc_feature    6635..7429 
                     /label="Kan/Neo"  
     misc_feature    7464..7766 
                     /label="SV40 ori"  
     misc_feature    7852..8329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="Diaph3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGGCAC CGGGCGCGCG CTCTCGGCCG GGACAGCAAG TCGTCGCGGA 
     1081 GGAAGGGCTT GCAGTCCGCG CCGCCCGCTG GCCCCTACGA GCCCGGGGAG AAGCGACCCA 
     1141 AGTTGCATTT AAATATTAGA ACACTGACAG ATGATATGCT GGACAAATTT GCCAGTATAA 
     1201 GAATTCCAGG GAGCAAGAAA GAGAGACCTC CCCTTCCCCA CCTGAAGACT GTGTCTGGGA 
     1261 TCAGTGACAG CTCATCACTG TCCTCAGAGA CAATGGAAAA CAACCCAAAG GCGCTGCCAG 
     1321 AGAGTGAAGT CTTGAAGCTT TTTGAGAAGA TGATGGAAGA TATGAATTTA AATGAAGATA 
     1381 AAAAGGCACC ATTGCGGGAA AAAGACTTCG GTATCAAAAA AGAAATGGTG ATGCAGTACA 
     1441 TTAATACTGC TTCTAAGACA GGAAGTCTTA GAAGTAGCCG ACAGATCTCA CCTCAGGAAT 
     1501 TTCTCCATGA GCTGAAAATG GGTTACACAG ACGAGAGACT TTTCACGTAT CTGGAGTCAC 
     1561 TCCGAGTATC ATTGACCAGT CATCCTGTGA GTTGGGTGCA AAGCTTTGGA CACGAGGGAC 
     1621 TCGGATTATT GCTGGACATT TTGGAAAAAC TAATTAATGG GCAAATCCAA GAAAAAGTTG 
     1681 TGAAGAAGAC TCAGCACAAA GTCATCCAGT GTCTGAGAGC CCTGATGAAC ACACAGTATG 
     1741 GCTTAGAAAG GATTATGAGT GACAAGAGGA GTCTTTCCTT GTTGGCAAAA GCCATGGATC 
     1801 CCAGGCAGCC CGCTATGATG GCAGACGTGG TGAAGCTTCT GTCTGCAGTG TGCATTGTCG 
     1861 GCGAGGAAAG CATCCTTGAA GAAGTGTTAG AAGCCTTGAC TTCAGCTGGA GAAGAAAGAA 
     1921 AGATTGACAG ATTTTTTTCC ATTGTGGAAG GCCTCCGGCA TAACTCAGTG AACCTGCAAG 
     1981 TTGCTTGTAT GCAGCTCATA AATGCTCTCG TTACATCTCC TGATGATTTG GACTTCAGGC 
     2041 TTCACCTCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATATTGCCA AACTTAAAGG 
     2101 GCATTAAGAA TGATGGCCTG GATATACAAC TTAAAGTCTT TGATGAGCAC AAAGAAGAAG 
     2161 ATTTGAGTGA GTTTTTCCAT CGCCTTGAAG ACATTAGAGC TGAACTTGAT GAAGCATCTG 
     2221 ATGTTTACAG CATGTTATGG GACACAGTTA AGGAAACTCG AGCAGAGGGA CATTTTCTTT 
     2281 CTATTCTTCA GCATCTCCTG CTCATTCGCA ATGATCGTTT TATAAGAGAG CAGTATTTCA 
     2341 AATTAATTGA TGAGTGTGTG TCACAGATTG TATTACATAG AGATGGAACG GACCCTGACT 
     2401 TCACATACAG AAAAAGACTA GATTTGGACT TAAGTCAGTT TGTAGATGTT TGCATAGATC 
     2461 AGGCCAAACT AGATGAGTGG GAAGAGAAAG CATCCGAACA TTGCAAGAAA TTTGAAAAAG 
     2521 AGTGTACTGA CCACCAAGAA ACCCAGGCTC AATTGCAGAA AAGAGAGGCA AAGATTAATG 
     2581 AGCTTCAAGC AGAGTTACAA GCTTTTAAAT CCCAGTTTGG TGCCTTGCCA CCTGGTACAA 
     2641 AAATTCCTTT GCAACCTTCA GTAGAAGGTG AAGCTGGCCC TTCAGCCCTT CCTCCTGCCC 
     2701 CACCAGCACT CAGTGGAGGA GTGCCGCCTC CCCCACCGCC CCCTCCTCCA CCACCCCCAC 
     2761 CACTCCCAGG AATGCCAATG CCATTTGGTG GCCCTGTACC ACCACCACCT CCTCTGGGAT 
     2821 TCCTGGGTGG GCAAAGCTCT ATTCCATTAA ACCTGCCATT TGGTTTGAAA CCAAAGAAAG 
     2881 AATTTAAGCC TGAAATCAGC ATGAGAAGAT TGAATTGGTT AAAGATCGGA CCAAATGAAA 
     2941 TGTCTGAGAA CTGCTTCTGG ATCAAAGTAA ATGAAAATAA GTATGAAAAT AGGGATTTGC 
     3001 TTTGTAAACT TGAGAACACT TTTTGTTGCC AAGAAAAAGA GAAAAGGAAT ACAAATGACT 
     3061 TTGATGAGAA GAAAGTTATT AAGAAGAGAA TGAAGGAACT TAAATTTCTA GATCCTAAAA 
     3121 TTGCTCAGAA CCTTTCAATC TTCCTGAGCT CCTTCCGGGT GCCATATGAG AAAATCAGGA 
     3181 CGATGATATT GGAAGTGGAT GAAACACAGT TGTCAGAGTC CATGATTCAG AACTTAATAA 
     3241 AGCACCTTCC TGATGAGGAG CAGTTGAAGT CATTGTCCCA GTTTAGAAGT GACTATAACA 
     3301 GTTTGTGTGA GCCTGAGCAG TTCGCTGTTG TGATGAGCAA TGTGAAGAGA CTCCGGCCAC 
     3361 GGCTCAGTGC TATTCTCTTT AAGCTTCAAT TTGAAGAGCA GGTGAACAAC ATCAAACCTG 
     3421 ACATCATGGC TGTCAGTACT GCCTGCGAGG AGATCAAGAA GAGCAAAGGC TTTAGCAAGT 
     3481 TGCTGGAACT TGTGTTGCTA ATGGGAAACT ACATGAATGC TGGCTCCCGG AATGCTCAAA 
     3541 CCTTCGGATT TGACCTTAGC TCTCTCTGTA AACTGAAGGA TACAAAATCT GCAGATCAGA 
     3601 AAACCACACT CCTCCATTTC CTGGTAGATG TATGTGAAGA AAAGCATGCT GACATCCTTC 
     3661 ACTTTGTGGA CGATTTGGCA CATTTAGACA AAGCTAGCAG AGTCTCTGTA GAAATGCTGG 
     3721 AAAAGAACGT GAAGCAGATG GGAAGGCAGC TTCAACAGCT TGAGAAGAAT TTGGAAACCT 
     3781 TTCCCCCTCC TGAGGACTTG CATGACAAGT TTGTGATAAA GATGTCCAGC TTCGTTATCA 
     3841 GTGCGAACGA GCAGTATGAA AAACTCTCCA CACTACTGGG CAGCATGACA CAATTGTACC 
     3901 AGAGTATAAT GGGCTACTAT GCTGTCGACA TGAAGAAGGT TTCCGTGGAA GAGTTTTTTA 
     3961 ATGATCTGAA CAACTTCAGA ACTTCATTTA TGCTAGCATT AAAGGAAAAC ATCAAAAAAC 
     4021 GAGAAGCAGC AGAAAAGGAG AAACGTGCCA GGATAGCGAA AGAGCGAGCA GAGAAAGAGC 
     4081 GACTTGAACG CCAGCAAGAG AAAAAGCGCT TACTAGAAAT GAAAACTGAG GGAGATGAGA 
     4141 CAGGAGTGAT GGATAGTCTG CTGGAGGCCT TGCAGTCAGG GGCTGCCTTC CGCGACAGAA 
     4201 GAAAAAGGAC ACCAAAGCTG AAAGATATTC GGCAGAGTCT CAGCCCGATG TCTCAGAGGC 
     4261 CTGTTCTCAA AGTTTGTAAC CATGAAAATC AGAAAATGCA GTTGACAGAA GGGTCACGTC 
     4321 CACACCACAG TATCAATTGC AACTCCACCA GGACTCCAGT CGCCAAGGAG CTTAATTATA 
     4381 ATCTAGACAC TCATGCGTCT ACAGGGAGGA TCAAGGCAGT TGAGAAGGAA GCCTGTAATG 
     4441 CAGAAAGCAA CAAAAAAAAG GAAATGGAAC TTCTTGGCTC TGTTGCTAAA AGCGAATCAG 
     4501 TTCCTGAAGT TGAAGCCCTG CTGGCAAGAT TACGAGCTTT AACGCGTACG CGGCCGCTCG 
     4561 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4621 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5341 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5401 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5461 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5521 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5581 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5641 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5701 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5761 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5821 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5881 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5941 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6001 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6061 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6121 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6181 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6241 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6301 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6361 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6421 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6481 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6541 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6601 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6661 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6721 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6781 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6841 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6901 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6961 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7021 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7081 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7141 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7201 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7261 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7321 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7381 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7441 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7501 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7561 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7621 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7801 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7981 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8041 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8101 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8161 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8221 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8281 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8341 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//