LOCUS       MR217977                8706 bp    DNA     circular  SYN 
DEFINITION  Magi2 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 2 (Magi2), transcript variant 1
ACCESSION   NM_001170746
VERSION     NM_001170746.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4863..4954 
                     /label="Myc-DDK"  
     misc_feature    4984..5569 
                     /label="PolyA signal"  
     misc_feature    5041..5060 
                     /label="Restriction Enzyme"  
     misc_feature    5845..6517 
                     /label="ColE1"  
     misc_feature    6947..7741 
                     /label="Kan/Neo"  
     misc_feature    7776..8078 
                     /label="SV40 ori"  
     misc_feature    8164..8641 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4845..4851 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4845 
                     /label="Magi2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAAAAGC TTGAAAAAGA AAAGCCACTG GACTAGCAAA GTCCACGAGA 
     1081 GTGTCATTGG CAGGAACCCG GAGGGCCAGC TGGGCTTTGA ACTGAAGGGG GGCGCGGAAA 
     1141 ACGGACAGTT CCCCTACCTG GGGGAGGTGA AGCCGGGCAA GGTGGCCTAT GAAAGCGGCA 
     1201 GCAAGTTGGT GTCCGAGGAG CTGCTGCTGG AGGTGAACGA GACCCCCGTG GCGGGGCTCA 
     1261 CCATCAGGGA CGTTCTGGCC GTGATCAAAC ACTGCAAGGA CCCCCTCCGG CTCAAGTGTG 
     1321 TCAAGCAAGG AGGAATTGTT GATAAAGACC TTCGTCACTA CCTCAACCTA AGATTTCAGA 
     1381 AGGGTTCTGT TGACCATGAA CTACAGCAAA TCATCCGTGA CAACCTCTAC CTCCGCACAG 
     1441 TGCCATGCAC CACAAGGCCA CATAAGGAGG GTGAGGTCCC TGGAGTGGAC TACATTTTCA 
     1501 TAACCGTTGA GGAGTTTATG GAATTGGAGA AAAGTGGTGC TCTCCTAGAA AGCGGGACCT 
     1561 ATGAAGACAA CTACTACGGT ACCCCGAAGC CTCCAGCTGA ACCAGCACCA TTATTAAATG 
     1621 TAACAGACCA GATACTTCCG GGAGCTACTC CAAGTGCTGA GGGGAAGCGG AAAAGAAATA 
     1681 AGTCAGTGAC CAACATGGAG AAAGCAAGTA TAGAGCCTCC AGAGGAGGAA GAAGAAGAAA 
     1741 GGCCTGTAGT CAATGGAAAC GGCGTGGTCA TAACCCCAGA ATCCAGTGAA CATGAAGACA 
     1801 AAAGTGCAGG TGCCTCAGGG GAGACACCCT CCCAGCCTTA CCCTGCACCC GTGTACAGCC 
     1861 AGCCCGAAGA GCTCAAGGAC CAGATGGACG ATACAAAGCC AACAAAGCCT GAGGAGAACG 
     1921 AGGACTCTGA TCCATTGCCT GATAACTGGG AAATGGCCTA CACAGAGAAG GGGGAAGTCT 
     1981 ACTTCATTGA CCATAACACA AAGACAACAT CATGGCTGGA TCCGCGACTT GCGAAAAAGG 
     2041 CTAAACCTCC AGAAGAGTGC AAAGAAAATG AGCTTCCATA TGGCTGGGAA AAAATCGATG 
     2101 ATCCTATATA TGGCACTTAC TATGTTGACC ACATAAATAG AAGAACACAG TTTGAAAACC 
     2161 CTGTCCTGGA AGCAAAAAGG AAGCTACAGC AACATAACAT GCCCCACACA GAACTTGGAG 
     2221 CAAAGCCCCT GCAGGCCCCA GGTTTCCGAG AAAAGCCACT CTTCACCCGG GATGCATCCC 
     2281 AGTTGAAGGG AACGTTCCTC AGCACCACCC TCAAAAAGAG CAACATGGGC TTTGGGTTTA 
     2341 CCATCATTGG TGGAGACGAG CCGGATGAGT TTCTACAGGT GAAAAGTGTG ATCCCGGATG 
     2401 GGCCTGCCGC ACAGGATGGG AAAATGGAGA CAGGTGATGT CATTGTCTAT ATTAATGAAG 
     2461 TTTGTGTCCT TGGACACACT CATGCAGATG TTGTCAAACT TTTCCAGTCT GTTCCTATTG 
     2521 GTCAGAGTGT CAACTTGGTG TTGTGTCGTG GCTACCCTTT GCCCTTTGAC CCTGAAGATC 
     2581 CTGCTAACAG CATGGTGCCA CCCCTTGCAA TAATGGAGAG GCCACCTCCG GTGATGGTCA 
     2641 ATGGAAGACA TAACTATGAA ACATACTTGG AATACATTTC TCGGACCTCA CAGTCGGTCC 
     2701 CAGATATTAC AGACCGGCCA CCTCATTCTT TGCACTCCAT GCCAGCTGAC GGCCAGCTAG 
     2761 ATGGCACGTA TCCACCACCC GTCCATGACG ACAATGTGTC TATGGCTTCG TCTGGAGCCA 
     2821 CTCAAGCTGA ACTTATGACC TTAACCATTG TGAAAGGTGC CCAGGGATTT GGCTTTACTA 
     2881 TTGCCGACAG TCCCACGGGA CAGCGGGTGA AACAAATCCT TGACATTCAG GGATGCCCTG 
     2941 GGCTGTGTGA AGGAGACCTC ATTGTTGAGA TCAACCAACA GAATGTACAG AACCTGAGCC 
     3001 ATACAGAAGT AGTGGATATA CTTAAGGACT GCCCCGTTGG AAGTGAGACT TCTTTAATCA 
     3061 TCCATCGAGG AGGTTTCTTT TCTCCATGGA AAACTCCAAA GCCTATGATG GACCGATGGG 
     3121 AGAACCAAGG CAGTCCACAA ACAAGTTTAT CTGCTCCGGC CGTCCCACAG AACCTGCCCT 
     3181 TCCCACCTGC CCTTCACAGG AGCTCCTTTC CTGATTCAAC AGAGGCCTTT GACCCACGGA 
     3241 AGCCTGACCC ATATGAGCTC TACGAGAAAT CGAGAGCCAT TTATGAAAGT AGGCAACAAG 
     3301 TGCCACCCAG GACCAGTTTT CGAATGGATT CCTCTGGTCC AGATTATAAG GAACTGGATG 
     3361 TTCACCTTCG GAGGATGGAG TCTGGATTTG GCTTTAGAAT CCTTGGGGGA GATGAACCTG 
     3421 GACAGCCTAT TTTGATCGGA GCCGTCATTG CCATGGGCTC AGCTGACAGA GACGGCCGTC 
     3481 TACACCCAGG AGATGAGCTT GTCTATGTCG ATGGGATCCC AGTGGCTGGC AAGACCCACC 
     3541 GCTATGTCAT CGACCTCATG CACCACGCGG CCCGCAATGG GCAGGTTAAC CTCACTGTGA 
     3601 GAAGAAAGGT GCTATGTGGA GGGGAGCCCT GCCCAGAGAA TGGGAGGAGT CCAGGCTCTG 
     3661 TATCAACTCA CCACAGCTCT CCGCGCAGTG ACTATGCCAC CTACTCCAAC AGCAACCACG 
     3721 CCGCCCCCAG CAGCAATGCC TCACCTCCTG AAGGCTTTGC CTCACACAGC TTGCAGACCA 
     3781 GTGATGTGGT CATTCACCGC AAAGAAAACG AAGGGTTTGG CTTCGTCATC ATCAGCTCTC 
     3841 TGAACAGGCC TGAGTCTGGA GCCACCATAA CTGTGCCCCA TAAAATTGGA CGAATCATTG 
     3901 ATGGGAGCCC TGCAGATCGC TGTGCCAAAC TCAAAGTGGG CGACCGTATC TTAGCAGTCA 
     3961 ACGGCCAGTC TATCATCAAC ATGCCTCACG CTGACATTGT GAAGCTCATC AAGGACGCCG 
     4021 GTCTCAGTGT CACCCTTCGC ATCATTCCTC AGGAGGAGCT CAACAGCCCA ACATCAGCAC 
     4081 CCAGTTCAGA GAAACAGAGC CCCATGGCCC AGCAGCACAG CCCTCTGGCC CAGCAGAGTC 
     4141 CTCTGGCCCA GCCAAGCCCC GCCACCCCCA ACAGCCCAGT CGCACAGCCA GCTCCTCCCC 
     4201 AACCTCTCCA GCTGCAAGGA CACGAAAATA GTTACAGGTC AGAAGTTAAA GCGAGGCAAG 
     4261 ATGTGAAGCC AGACATCCGG CAGCCTCCCT TCACAGACTA CAGGCAGCCC CCGCTGGACT 
     4321 ACAGGCAGCC CCCGGGAGGA GACTACTCAC AGCCCCCACC CTTGGACTAC AGGCAGCACT 
     4381 CTCCAGACAC CAGGCAGTAC CCTCTGTCAG ACTACAGGCA GCCACAGGAT TTTGATTATT 
     4441 TCACTGTGGA CATGGAGAAA GGAGCCAAAG GATTTGGATT CAGCATTCGT GGAGGAAGGG 
     4501 AATACAAGAT GGATCTGTAT GTGTTGAGAT TGGCAGAGGA TGGGCCAGCC ATAAGGAACG 
     4561 GCAGGATGAG GGTAGGAGAT CAGATCATTG AAATAAATGG GGAAAGCACA CGAGACATGA 
     4621 CCCACGCCAG AGCAATAGAA CTCATCAAGT CTGGAGGAAG AAGAGTGCGG CTGCTGCTGA 
     4681 AGAGAGGCAC GGGGCAGGTC CCGGAGTATG GAATGGTACC TTCCAGCCTC TCCATGTGCA 
     4741 TGAAAAGTGA CAAGCATGGG TCCCCATATT TCTACTTACT GGGCCACCCT AAAGACACGA 
     4801 CGAACCCCAC GCCTGGAGTG CTGCCGCTGC CGCCGCCCCA GGCCTGCCGG AAGACGCGTA 
     4861 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4921 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4981 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5041 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5101 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5161 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5221 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5281 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5341 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5401 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5461 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5521 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5581 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5641 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5701 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5761 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5821 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5881 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5941 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6001 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6061 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6121 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6181 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6241 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6301 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6361 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6421 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6481 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6541 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6601 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6661 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6721 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6781 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6841 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6901 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6961 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7021 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7081 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7141 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7201 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7261 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7321 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7381 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7441 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7501 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7561 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7621 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7681 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7741 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7801 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7861 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7921 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8041 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8101 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8161 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8221 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8281 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8341 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8401 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8461 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8521 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8581 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8641 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8701 AATTTT
//