LOCUS       MR217837                8274 bp    DNA     circular  SYN 
DEFINITION  Slc4a7 (Myc-DDK-tagged) - Mouse solute carrier family 4, sodium bicarbonate cotransporter, member 7 (Slc4a7)
ACCESSION   NM_001033270
VERSION     NM_001033270.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4431..4522 
                     /label="Myc-DDK"  
     misc_feature    4552..5137 
                     /label="PolyA signal"  
     misc_feature    4609..4628 
                     /label="Restriction Enzyme"  
     misc_feature    5413..6085 
                     /label="ColE1"  
     misc_feature    6515..7309 
                     /label="Kan/Neo"  
     misc_feature    7344..7646 
                     /label="SV40 ori"  
     misc_feature    7732..8209 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4413..4419 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4413 
                     /label="Slc4a7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCAGAC GGGGCCGGCG AGCAGATGAG ACCGCTACTC ACGCGGGGTC 
     1081 CAGATGAAGA AGCTGTTGTG GATCTTGGCA AAACTAGCTC AACTGTGAAC ACCAAGTTTG 
     1141 AAAAAGAAGA ATTAGAAAGT CATCGAGCTG TATATGTTGG TGTTCATGTA CCATTTAGTA 
     1201 AAGAGAGTCG TCGGCGTCAC AAGCATCGAG GGCACAAACA TCACCACCGG AGAAGAAAAG 
     1261 ACAAAGACTC AGATAAAGAA GATGGACGGG AATCTCCTTC TTATGACACG CCATCGCAGA 
     1321 GGGTGCAGTT CATCCTCGGG ACTGAAGACG ACGATGAGGA GCACATTCCC CACGACCTCT 
     1381 TCACAGAGAT GGATGAGCTC TGCTACAGAG ACGGAGAAGA GTACGAGTGG AAGGAGACGG 
     1441 CCAGGTGGCT GAAATTTGAA GAGGATGTTG AAGATGGTGG TGACCGATGG AGTAAACCCT 
     1501 ATGTGGCAAC TCTGTCTCTA CACAGTCTCT TTGAATTGAG GAGTTGTATC CTGAATGGAA 
     1561 CAGTTATGCT GGATATGAGA GCAAGCACTC TTGATGAAAT TGCAGATATG GTGTTAGACA 
     1621 ACATGATAGC ATCTGGCCAG TTAGATGACT CCATAAGGGA GAACGTCAGA GAAGCTCTTC 
     1681 TGAAGAGACA TCATCATCAA AATGAGAAAA GGTTCACGAG TCGGATTCCC CTGGTTCGAT 
     1741 CCTTTGCAGA TATAGGCAAG AAACATTCTG ACCCTCACTT GCTTGAAAGG AATGGGATTT 
     1801 TGGCTTCTCC ACAGTCTGCT CCTGGAAACT TGGACAATAG TAAAAGTGGT GAAATGAAAG 
     1861 GTAACGGAAG TGGAGGAAGC AGAGAAAATA GTACTGTTGA CTTTAGCAAG GTTGACATGA 
     1921 ATTTCATGAG GAAAATTCCT ACAGGAGCTG AGGCATCCAA TGTTTTGGTA GGAGAAGTGG 
     1981 ACTTCTTGGA GAGACCGATC ATTGCATTTG TGAGACTGGC TCCTGCAGTT CTCCTCTCAG 
     2041 GGTTGACTGA GGTCCCTGTG CCCACTAGGT TTTTGTTTCT GTTACTGGGC CCAGCAGGGA 
     2101 AAGCCCCACA GTACCATGAA ATTGGCAGAT CCATAGCAAC TCTCATGACA GATGAGATTT 
     2161 TTCATGATGT GGCTTATAAA GCAAAAGATC GTAATGACCT CCTATCTGGA ATTGATGAAT 
     2221 TCTTAGATCA AGTAACTGTT CTTCCTCCAG GAGAGTGGGA TCCTTCCATA CGCATAGAGC 
     2281 CTCCAAAAAG TGTTCCTTCT CAGGAAAAAA GGAAGATTCC TGTGTTTCCT AATGGATCTG 
     2341 CTGCAATGTC TGTTGACCCT CCCAAGGAGG ATGATCACCA TGCTGGGCCT GAGCTGCAGA 
     2401 GGACTGGACG GCTTTTTGGT GGTTTGATAC TTGACATCAA AAGGAAAGCA CCTTTTTTCT 
     2461 TGAGTGACTT CAAGGATGCA TTAAGTCTGC AGTGCCTGGC CTCGATTCTT TTCCTATACT 
     2521 GTGCCTGTAT GTCTCCTGTA ATCACTTTTG GAGGGCTGCT TGGAGAAGCT ACAGAAGGCA 
     2581 GAATAAGTGC AATAGAGTCT CTTTTTGGAG CGTCATTAAC TGGGATTGCC TATTCATTGT 
     2641 TTGCTGGGCA ACCTCTAACA ATACTGGGGA GCACAGGTCC AGTCCTAGTG TTTGAAAAAA 
     2701 TTTTATTTAA ATTCTGTAGA GATTATCACC TTTCCTATCT ATCATTAAGA ACCAGTATTG 
     2761 GTCTGTGGAC ATCTTTCTTG TGCATTGTAT TGGTCGCAAC AGATGCAAGC AGCCTTGTTT 
     2821 GTTACATTAC TCGGTTCACA GAAGAAGCTT TTGCGGCTCT CATTTGTATC ATATTCATCT 
     2881 ATGAAGCTCT GGAGAAGCTC TTTCACTTAG GAGAAATATA TGCATTTAAT ATGCACAACA 
     2941 ACTTGGATGA ATTGACCAGT TACACATGTG TATGTGCTGA GCCATCTAAC CCTAGCAACG 
     3001 AAACTCTAGA ACTGTGGAAG AGGAAGAATA TAACAGCATA CAGTGTTTCC TGGGGCAACC 
     3061 TTACTGTTTC TGAGTGTAAG ACCTTCCATG GTATGTTTGT GGGATCAGCT TGTGGGCCTC 
     3121 ATGGACCTTA TGTTCCTGAC GTGCTCTTCT GGTGTGTCGT CTTGTTTTTC ACGACGTTCT 
     3181 TTCTGTCTTC ATTCCTCAAG CAGTTTAAGA CCAAGAGATA TTTTCCTACC AAGGTACGAT 
     3241 CAACAATCAG TGACTTTGCT GTATTTCTCA CAATAGTAAT AATGGTTGCA ATTGACTACC 
     3301 TTGTAGGAAT TCCATCTCCT AAACTTCATG TGCCTGAAAA GTTCGAGCCT ACTGATCCAA 
     3361 GTAGGGGCTG GATCATAAGC CCTTTGGGAG ATAACCCTTG GTGGACCTTA CTAATTGCAG 
     3421 CAGTTCCAGC TCTCCTTTGT ACTATTCTCA TCTTTATGGA CCAGCAGATC ACAGCTGTAA 
     3481 TCATCAACAG AAAAGAACAC AAACTCAAGA AAGGAGCTGG CTACCACCTT GACTTGCTCA 
     3541 TGGTTGGTGT CATGTTGGGA GTCTGCTCTA TCATGGGCTT GCCGTGGTTT GTGGCTGCGA 
     3601 CAGTGTTGTC TATAAGTCAT GTCAACAGCT TGAAAGTGGA GTCTGAGTGT TCTGCTCCAG 
     3661 GGGAGCAGCC CAAGTTTCTG GGAATTCGTG AACAGCGGGT TACAGGGCTG ATGATTTTTA 
     3721 TCCTGATGGG CCTCTCTGTG TTCATGACTT CAGTATTAAA GTTTATCCCA ATGCCAGTTT 
     3781 TGTACGGTGT TTTTCTTTAT ATGGGAGTTT CATCGTTGAA AGGAATTCAG TTTTTTGACC 
     3841 GTATCAAGTT ATTTGGAATG CCTGCCAAGC ACCAGCCCGA CCTAATCTAC CTCCGCTATG 
     3901 TCCCCCTGTG GAAGGTGCAC GTGTTCACGG TCGTCCAGCT GACCTGCCTA GTCCTGCTGT 
     3961 GGGTGATCAA AGCCTCTGCT GCTGCAGTAG TTTTTCCCAT GATGGTTCTT GCATTAGTCT 
     4021 TTGTGCGCAA GCTCATGGAT CTCTGTTTCA CAAAGAGAGA ACTCAGTTGG CTTGATGACC 
     4081 TCATGCCAGA AAGTAAGAAA AAGAAAGAAG ATGACAAAAA GAAAAAAGAG AAAGAGGAAG 
     4141 CTGAACGGAT GCTTCAGGAT GATGAGGACA CTGTGCACCT CCCATTCGAA AGAGGGAGTC 
     4201 TCCTGCAGAT TCCAGTGAAA ACTCTAAAAT ATAGTATTGA CCCTTCAGTT GTTAACATAT 
     4261 CAGATGAAAT GGCCAAAACT GCCCAGTGGA AGGCACTTTC CATGAATACT GAGAATGCCA 
     4321 AAGTAACGAG ACCTAACACG AGCCCTGAAA AGCCTGTGAG TGTGACAATA AATTTCGAAG 
     4381 ATGAACCGTC AAAAAAATAC ATGGATGCTG AAACTTCATT GACGCGTACG CGGCCGCTCG 
     4441 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4501 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4561 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4621 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4681 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4741 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4801 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4861 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4921 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4981 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5041 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5101 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5161 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5221 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5281 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5341 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5401 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5461 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5521 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5581 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5641 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5701 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5761 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5821 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5881 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5941 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6001 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6061 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6121 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6181 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6241 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6301 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6361 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6421 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6481 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6541 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6601 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6661 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6721 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6781 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6841 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6901 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6961 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7021 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7081 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7141 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7201 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7261 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7321 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7381 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7441 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7501 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7561 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7681 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7741 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7801 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7861 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7921 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7981 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8041 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8101 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8161 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8221 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//