LOCUS       MR217696                8808 bp    DNA     circular  SYN 
DEFINITION  Synj1 (Myc-DDK-tagged) - Mouse synaptojanin 1 (Synj1), transcript variant 2
ACCESSION   NM_001045515
VERSION     NM_001045515.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4965..5056 
                     /label="Myc-DDK"  
     misc_feature    5086..5671 
                     /label="PolyA signal"  
     misc_feature    5143..5162 
                     /label="Restriction Enzyme"  
     misc_feature    5947..6619 
                     /label="ColE1"  
     misc_feature    7049..7843 
                     /label="Kan/Neo"  
     misc_feature    7878..8180 
                     /label="SV40 ori"  
     misc_feature    8266..8743 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4947..4953 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4947 
                     /label="Synj1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTTCAGC AAAGGATTTC GAATCTACCA CAAATTGGAT CCCCCACCGT 
     1081 TCAGCCTCAT AGTGGAAACT AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG 
     1141 TGGCTGTGCT CTCATCTGCA GAAAAGGAGG CTATTAAAGG CACATATGCC AAAGTACTGG 
     1201 ATGCATACGG ACTTTTGGGA GTTTTACGAT TAAATCTTGG TGATACCATG CTGCACTATC 
     1261 TGGTCCTAGT CACTGGATGC ATGTCTGTGG GGAAAATCCA AGAATCTGAA GTTTTCCGAG 
     1321 TTACTTCCAC TGAGTTTATA TCATTGCGAG TTGATGCTTC AGATGAGGAC CGCATTTCGG 
     1381 AAGTACGAAA AGTTTTGAAC TCGGGAAACT TTTATTTTGC ATGGTCTGCA TCTGGAGTCA 
     1441 GCTTAGATCT GAGTCTGAAT GCACATCGAA GCATGCAAGA GCACACAACC GACAATAGGT 
     1501 TTTTCTGGAA TCAGTCTCTG CATTTGCATC TCAAGCACTA TGGTGTGAAC TGCGATGACT 
     1561 GGTTATTACG GCTCATGTGT GGGGGAGTAG AGATTAGAAC CATTTATGCT GCCCATAAAC 
     1621 AGGCAAAAGC TTGCCTCATC TCAAGACTAA GCTGTGAACG AGCTGGGACA AGGTTCAATG 
     1681 TCCGGGGAAC CAACGATGAT GGTCATGTTG CCAACTTTGT AGAGACAGAG CAGGTTATAT 
     1741 ACTTAGATGA CTGTGTCTCT TCCTTCATAC AAATCCGAGG ATCTGTTCCA TTGTTCTGGG 
     1801 AGCAACCAGG GTTGCAAGTG GGATCCCATC GTGTTCGTAT GTCAAGGGGA TTTGAAGCCA 
     1861 ATGCGCCTGC CTTTGACAGG CATTTCCGAA CACTTAAGGA CTTATATGGT AAACAGATAG 
     1921 TAGTGAATCT GCTTGGGTCT AAGGAAGGCG AGCATATGCT GAGCAAGGCT TTCCAGAGTC 
     1981 ATTTAAAAGC CTCTGAACAC GCTTCTGATA TCCACATGGT GTCTTTTGAC TATCATCAAA 
     2041 TGGTTAAAGG AGGAAAGGCA GAAAAACTAC ACAGTATTCT CAAACCACAA GTCCAGAAGT 
     2101 TTCTAGATTA TGGATTTTTT TATTTCGATG GCAGTGAAGT TCAAAGGTGC CAGAGTGGTA 
     2161 CAGTTCGAAC AAACTGCTTG GATTGTCTGG ATAGAACAAA TAGTGTGCAG GCATTCCTTG 
     2221 GCTTAGAGAT GCTCGCTAAG CAGCTGGAAG CTCTGGGCTT GGCTGAGAAG CCTCAGCTGG 
     2281 TGACCCGCTT CCAAGAAGTT TTCCGGTCCA TGTGGTCTGT GAATGGCGAC TCAATCAGTA 
     2341 AAATATATGC AGGCACCGGG GCCCTGGAAG GGAAGGCCAA GTTAAAAGAT GGTGCGCGAT 
     2401 CTGTCACCAG AACCATCCAG AATAACTTCT TCGACAGCTC CAAGCAGGAA GCCATTGATG 
     2461 TCCTGCTCCT GGGAAATACT CTCAACAGCG ATTTAGCTGA CAAAGCCCGA GCGCTTCTAA 
     2521 CTACTGGAAG TTTGCGTGTT TCTGAACAGA CATTACAGTC AGCATCTTCC AAAGTCTTAA 
     2581 AGAACATGTG TGAGAACTTC TACAAATACT CAAAGCCCAA GAAGATCCGA GTGTGTGTTG 
     2641 GCACCTGGAA TGTGAACGGC GGGAAGCAGT TCCGCAGCAT AGCGTTCAAG AACCAGACGC 
     2701 TCACAGACTG GCTTCTGGAC GCTCCCAAGT TAGCCGGCAT CCAGGAGTTT CAAGATAAGA 
     2761 GAAGTAAGCC AACTGATATA TTTGCAATTG GCTTTGAAGA AATGGTGGAG CTGAATGCTG 
     2821 GGAACATTGT GAATGCAAGC ACAACCAACC AGAAGCTGTG GGCAGTGGAG CTGCAGAAGA 
     2881 CCATCTCCAG GGACAACAAG TATGTCCTGC TGGCCTCGGA GCAGCTGGTG GGTGTCTGCC 
     2941 TGTTTGTCTT TATCAGACCA CAGCACGCTC CTTTTATCAG GGATGTTGCA GTTGATACTG 
     3001 TGAAAACTGG CATGGGAGGT GCAACTGGAA ACAAGGGCGC CGTTGCAATT CGGATGCTCT 
     3061 TCCACACCAC CAGCCTTTGC TTCGTCTGCA GCCACTTTGC TGCAGGGCAG TCCCAAGTCA 
     3121 AAGAGAGAAA TGAAGATTTT GTAGAAATAG CACGAAAACT GAGTTTCCCC ATGGGACGAA 
     3181 TGCTCTTCTC CCATGACTAT GTATTTTGGT GTGGTGATTT CAACTATCGA ATTGACCTTC 
     3241 CGAATGAAGA GGTTAAAGAG CTCATAAGAC AACAAAACTG GGATTCTCTG ATCGCTGGGG 
     3301 ATCAGCTCAT CAACCAGAAA AATGCAGGAC AGATCTTTAG AGGATTCTTA GAGGGAAAAG 
     3361 TAACGTTTGC TCCAACCTAT AAATATGACT TGTTTTCTGA AGACTATGAC ACCAGTGAGA 
     3421 AGTGTCGCAC CCCTGCTTGG ACAGACCGTG TCCTCTGGAG AAGGAGGAAG TGGCCTTTTG 
     3481 ACAGATCAGC TGAAGATTTA GATCTCCTGA ATGCTAGTTT CCAAGATGAA AGTAAAATTC 
     3541 TTTATACATG GACCCCTGGC ACCTTGCTGC ACTATGGAAG AGCCGAGCTG AAGACTTCTG 
     3601 ACCATAGGCC CGTCGTTGCC TTGATTGACA TAGATATATT TGAAGTTGAA GCTGAAGAGA 
     3661 GACAAAAAAT TTATAAAGAA GTTATTGCCG TCCAGGGCCC ACCAGATGGC ACAGTGCTGG 
     3721 TCTCAATCAA AAGTTCTGCA CAAGAAAGTA CTTTTTTTGA TGATGCTTTG ATTGATGAGC 
     3781 TTCTGCGGCA GTTTGCACAC TTTGGTGAAG TTATACTCAT AAGATTTGTA GAAGATAAAA 
     3841 TGTGGGTTAC ATTTTTGGAG GGAAGCTCTG CCTTGAATGC TCTGAGCCTA AATGGGAAAG 
     3901 AGCTATTAAA TCGGACTATA ACAATTACTT TAAAAAGTCC AGACTGGATC AAACATTTGG 
     3961 AAGAAGAGAT GAGTTTAGAG AAAATCAGTG TCACGTTGCC CTCATCAGCA AGCTCCACCC 
     4021 TGCTTGGTGA AGACGCAGAG GTCGCAGCAG ACTTTGACAT GGAAGGTGAC GTCGATGACT 
     4081 ACAGCGCTGA AGTAGAGGAG CTTCTTCCTC AGCATCTGCA GCCGTCCTCA AGTTCTGGCC 
     4141 TGGGCACTTC TCCAAGTTCT TCACCCCGGA CCAGTCCCTG CCAGTCCCCC ACAGTACCAG 
     4201 AGTACTCCGC CCCTTCTCTT CCCATCAGAC CTAGCCGAGC ACCGTCAAGA ACTCCAGGAC 
     4261 CTCCAAGTTC GCAGGGCTCT CCAGTTGACA CTCAGCCAGC GGCCCAGAAG GATTCTTCCC 
     4321 AGACTCTAGA GCCCAAGAGA CCACCCCCTC CCCGCCCAGT CGCTCCTCCG GCACGTCCTG 
     4381 CCCCACCACA GAGACCACCT CCACCTTCAG GGGCTAGGAG TCCTGCGCCT GCTAGAAAAG 
     4441 AATTTGGAGG TGTTGGAGCC CCTCCCAGTC CCGGGGTTGC TAGGAGAGAG ATAGAAGCAC 
     4501 CCAAAAGCCC TGGAACAGCA CGGAAAGATA ATATAGGGCG TAATCAGCCT TCCCCTCAAG 
     4561 CCGGACTTGC AGGCCCAGGA CCCGCTGGAT ACGGTGCGGC TAGACCGACA ATTCCAGCTC 
     4621 GTGCTGGAGT GATCAGCGCC CCTCAGAGCC AGGCTCGGGT ATGTGCTGGA AGGCCGACTC 
     4681 CTGACAGCCA AAGCAAGCCC TCGGAGACAT TGAAAGGTCC TGCTGTCCTT CCAGAACCAC 
     4741 TGAAGCCTCA GGCTGCATTT CCTCAGCAGC CTTCTCTGCC CACACCTGCT CAAAAGTTAC 
     4801 AGGACCCGCT TGTCCCCATA GCAGCACCTA CTATGCCTCC CTCTGGCCCC CAACCAAATT 
     4861 TGGAAACCCC CCCACAGCCC CCACCTCGGA GCAGGTCATC TCAAAGCTTG CCTTCAGACT 
     4921 CCTCACCACA GCTGCAGCAG GAGCAACCAA CAGGGACGCG TACGCGGCCG CTCGAGCAGA 
     4981 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5041 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5101 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5161 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5221 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5281 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5341 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5401 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5461 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5521 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5581 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5641 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5701 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5761 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5821 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5881 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5941 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6001 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6061 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6121 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6181 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6241 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6301 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6361 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6421 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6481 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6541 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6601 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6661 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6721 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6781 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6841 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6901 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6961 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7021 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7081 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7141 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7201 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7261 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7321 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7381 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7441 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7501 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7561 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7621 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7681 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7741 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7801 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7861 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7921 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7981 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8041 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8101 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8161 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8221 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8281 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8341 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8401 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8461 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8521 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8581 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8641 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8701 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8761 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//