LOCUS       MR217686                7983 bp    DNA     circular  SYN 
DEFINITION  Pear1 (Myc-DDK-tagged) - Mouse platelet endothelial aggregation receptor 1 (Pear1), transcript variant 1
ACCESSION   NM_028460
VERSION     NM_028460.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4140..4231 
                     /label="Myc-DDK"  
     misc_feature    4261..4846 
                     /label="PolyA signal"  
     misc_feature    4318..4337 
                     /label="Restriction Enzyme"  
     misc_feature    5122..5794 
                     /label="ColE1"  
     misc_feature    6224..7018 
                     /label="Kan/Neo"  
     misc_feature    7053..7355 
                     /label="SV40 ori"  
     misc_feature    7441..7918 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4122..4128 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4122 
                     /label="Pear1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACTTTGT CCCCTCCTCC TCCTGGCCCT AGGCCTGCGT CTGACTGGGA 
     1081 CACTCAACTC CAATGATCCC AATGTCTGTA CCTTTTGGGA AAGCTTCACC ACGACCACTA 
     1141 AGGAGTCCCA CCTGCGCCCC TTCAGCCTGC TCCCAGCTGA GTCCTGCCAC AGGCCCTGGG 
     1201 AGGACCCCCA CACCTGTGCC CAGCCTACGG TTGTCTACCG GACTGTGTAC CGTCAGGTGG 
     1261 TGAAGATGGA CTCCCGCCCA CGCCTGCAGT GCTGTAGGGG TTACTACGAG AGCAGAGGGG 
     1321 CCTGTGTCCC ACTCTGTGCC CAGGAGTGTG TCCATGGTCG CTGTGTGGCT CCGAATCAGT 
     1381 GCCAGTGTGC ACCAGGCTGG CGGGGTGGCG ACTGCTCCAG CGAGTGTGCC CCGGGAATGT 
     1441 GGGGACCACA GTGTGACAAG TTCTGCCACT GTGGCAACAA CAGTTCCTGT GATCCCAAGA 
     1501 GTGGGACGTG CTTTTGCCCC TCTGGCCTGC AGCCCCCCAA CTGCCTTCAG CCCTGCCCTG 
     1561 CCGGCCACTA TGGTCCTGCC TGCCAGTTTG ATTGCCAGTG CTATGGGGCA TCCTGTGACC 
     1621 CCCAGGATGG AGCCTGTTTC TGCCCTCCAG GGAGAGCAGG ACCCAGCTGT AATGTGCCCT 
     1681 GTTCACAGGG CACTGATGGC TTCTTCTGCC CCAGAACCTA TCCTTGCCAA AATGGAGGTG 
     1741 TTCCTCAGGG CTCCCAAGGC TCCTGCAGCT GCCCACCGGG CTGGATGGGT GTCATTTGTT 
     1801 CCCTGCCATG CCCAGAGGGT TTCCATGGAC CCAACTGTAC TCAGGAATGT CGCTGCCACA 
     1861 ACGGTGGCCT CTGTGACAGG TTTACTGGGC AGTGCCACTG TGCTCCTGGC TATATCGGGG 
     1921 ATCGGTGCCA AGAAGAGTGC CCCGTGGGCC GCTTCGGTCA AGACTGTGCT GAGACCTGTG 
     1981 ACTGTGCTCC TGGCGCCCGT TGCTTTCCTG CTAATGGCGC GTGTCTGTGT GAACATGGCT 
     2041 TCACAGGCGA CCGCTGCACT GAGCGCCTCT GTCCGGATGG CCGCTATGGT CTGAGCTGCC 
     2101 AGGAGCCCTG CACCTGCGAC CCAGAACACA GTCTCAGCTG CCACCCGATG CACGGCGAGT 
     2161 GCTCCTGCCA GCCAGGTTGG GCGGGCCTCC ACTGCAACGA GAGCTGCCCT CAGGACACGC 
     2221 ATGGCCCGGG CTGCCAGGAG CACTGCCTCT GTCTGCACGG AGGGCTCTGC CTTGCCGACA 
     2281 GCGGCCTCTG CCGGTGCGCG CCGGGATACA CGGGACCTCA CTGCGCTAAC CTATGTCCAC 
     2341 CGGACACTTA CGGGATCAAC TGTTCCTCCC GCTGCTCCTG TGAAAATGCC ATTGCCTGCT 
     2401 CTCCCATCGA CGGCACGTGC ATCTGCAAGG AAGGTTGGCA GCGTGGTAAC TGCTCTGTTC 
     2461 CCTGTCCCCT TGGCACCTGG GGCTTCAATT GCAATGCCAG TTGCCAGTGT GCCCACGACG 
     2521 GAGTCTGCAG CCCCCAAACT GGAGCCTGTA CTTGCACCCC TGGGTGGCAT GGTGCTCACT 
     2581 GCCAGCTTCC CTGCCCGAAG GGACAGTTTG GTGAAGGCTG TGCCAGTGTC TGTGACTGTG 
     2641 ACCACTCTGA TGGCTGTGAC CCTGTTCATG GACAGTGCCG ATGTCAGGCT GGTTGGATGG 
     2701 GCACACGCTG CCACCTGCCT TGCCCGGAGG GCTTTTGGGG AGCCAACTGC AGTAACACCT 
     2761 GTACCTGCAA GAATGGTGGT ACCTGTGTGT CTGAGAATGG CAACTGCGTG TGCGCACCAG 
     2821 GGTTCCGAGG CCCCTCCTGC CAGAGGCCCT GCCCGCCTGG TCGCTATGGC AAACGCTGTG 
     2881 TGCAATGCAA GTGTAACAAC AACCATTCTT CCTGCCACCC ATCGGACGGG ACCTGCTCCT 
     2941 GCCTGGCGGG CTGGACAGGC CCTGACTGCT CCGAGGCATG TCCCCCAGGC CACTGGGGAC 
     3001 TCAAATGCTC CCAACTCTGC CAGTGTCATC ATGGTGGGAC CTGCCACCCC CAGGATGGGA 
     3061 GCTGTATCTG CACGCCAGGC TGGACTGGAC CCAACTGCTT GGAAGGCTGC CCACCAAGAA 
     3121 TGTTTGGTGT CAACTGCTCC CAGCTATGTC AGTGTGATCT CGGAGAGATG TGCCACCCAG 
     3181 AGACTGGGGC TTGTGTCTGT CCCCCAGGAC ACAGTGGTGC AGACTGCAAA ATGGGAAGCC 
     3241 AGGAGTCCTT CACCATAATG CCCACCTCTC CCGTGACCCA TAACTCACTG GGTGCAGTGA 
     3301 TTGGCATTGC AGTACTGGGA ACCCTCGTGG TGGCCCTGAT AGCACTGTTC ATTGGCTACC 
     3361 GCCAGTGGCA AAAGGGCAAG GAACATGAGC ACTTGGCAGT GGCTTACAGC ACTGGGCGGC 
     3421 TGGATGGCTC TGATTACGTC ATGCCAGATG TCTCTCCGAG CTATAGTCAC TACTACTCCA 
     3481 ACCCCAGCTA CCACACACTG TCTCAGTGTT CTCCTAACCC CCCACCCCCT AACAAGGTCC 
     3541 CAGGCAGTCA GCTCTTTGTC AGCTCCCAGG CCCCTGAGCG GCCAAGCAGA GCCCACGGGC 
     3601 GTGAGAACCA TGTCACACTG CCCGCTGACT GGAAGCACCG CCGGGAGCCC CATGAAAGAG 
     3661 GCGCCAGCCA CCTGGACCGA AGCTATAGCT GTAGCTATAG CCACAGGAAT GGCCCGGGAC 
     3721 CATTCTGTCA TAAAGGTCCC ATCTCTGAAG AGGGACTAGG GGCAAGCGTT ATGTCCCTGA 
     3781 GCAGTGAGAA CCCCTATGCT ACCATCCGAG ACCTGCCCAG CCTGCCTGGG GAACCCCGAG 
     3841 AAAGCGGCTA TGTGGAGATG AAAGGACCTC CATCAGTGTC CCCTCCCAGG CAGTCTCTTC 
     3901 ATCTCCGGGA CAGGCAGCAG CGGCAACTGC AGCCACAGAG GGACAGTGGC ACCTATGAGC 
     3961 AGCCCAGCCC CTTGAGCCAT AATGAAGAGT CTTTGGGCTC CACGCCCCCG CTTCCTCCAG 
     4021 GCCTGCCTCC TGGTCACTAC GACTCCCCCA AGAACAGCCA TATCCCTGGA CACTATGACT 
     4081 TGCCTCCAGT ACGGCATCCT CCATCCCCTC CATCCCGGCG CCAGGACCGC ACGCGTACGC 
     4141 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4201 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4261 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4321 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4381 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4441 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4501 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4561 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4621 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4681 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4741 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4801 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4861 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4921 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4981 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5041 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5101 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5161 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5221 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5281 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5341 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5401 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5461 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5521 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5581 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5641 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5701 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5761 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5821 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5881 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5941 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6001 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6061 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6121 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6181 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6241 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6301 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6361 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6421 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6481 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6541 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6601 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6661 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6721 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6781 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6841 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6901 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6961 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7021 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7081 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7141 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7201 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7261 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7321 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7381 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7441 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7501 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7561 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7621 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7681 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7741 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7801 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7861 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7921 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7981 TTT
//