LOCUS       MR217670                9195 bp    DNA     circular  SYN 
DEFINITION  Fyco1 (Myc-DDK-tagged) - Mouse FYVE and coiled-coil domain containing 1 (Fyco1), transcript variant 2
ACCESSION   NM_001110253
VERSION     NM_001110253.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5352..5443 
                     /label="Myc-DDK"  
     misc_feature    5473..6058 
                     /label="PolyA signal"  
     misc_feature    5530..5549 
                     /label="Restriction Enzyme"  
     misc_feature    6334..7006 
                     /label="ColE1"  
     misc_feature    7436..8230 
                     /label="Kan/Neo"  
     misc_feature    8265..8567 
                     /label="SV40 ori"  
     misc_feature    8653..9130 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5334..5340 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5334 
                     /label="Fyco1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCTAGC AGCACTGAGA CTCAGCTCCA GAGGATCATC CGTGATCTAC 
     1081 AAGATGCAGC GACAGAACTA AGCCATGAAT TTAAGGAAGG CGGAGAGCCA ATCACAGATG 
     1141 ACAGCACCAG TTTGCACAAG TTTTCTTACA AACTTGAGTA TCTTCTGCAA TTTGATCAGA 
     1201 AGGAGAAGGC GAGCCTCCTG GGAAGCAAGA AAGACTACTG GGATTACTTT TGTGCGTGCC 
     1261 TAGCCAAGGT GAAAGGAGCA AATGATGGCA TTCGATTTGT CAGGTCCATC TCTGAGCTCC 
     1321 GAACATCTCT GGGGAAAGGG AGAGCCTTCA TTCGCTACTC TTTGGTGCAC CAGAGGCTGG 
     1381 CAGACACCTT GCAGCAGTGC TTCATGAACA CTAAAGTGAC CAGTGACTGG TATTATGCAA 
     1441 GAAGCCCCTT TCTGAAGCCA AAGCTGAGCT CTGACATTGT GGGTCAACTG TATGAGCTGA 
     1501 CTGAGGTTCA GTTTGATCTA GCACCAAGAG GCTATGACTT GGATGCCGCC TGGCCAACAT 
     1561 TTGCCAGGAG GACGCTAGCC ACCAGCACGT CTGCTTACAT GTGGAAACCC CCCAGCCGAA 
     1621 GCTCCAGCAT GAGTAGTTTA GTGAGCAACT ACTTGCAGAC TCAGGAGATG GCCTCCAGTC 
     1681 TTGACCTGAA TTGTTCTCTA AACAATGAAG CACTAGAAAG CTTCGACGAG ATGCGGCTGG 
     1741 AGCTGGACCA GTTGGAGGTT CGGGAGAAAC AGCTACAAGA ACGTGTACAG CAGCTAGACA 
     1801 GGGAGAACCA AGCACTGCGA ATGTTGGTCA GCAGACAAGG GGGGCAGCTT CAGGTAGAGA 
     1861 AGGAGATGGG GTACCTTGCA GTCGAGGACA GCATTGGCCT TGTGAGCCTG GTAGCAGAAC 
     1921 TCCAGAAGCA GGGGGACGTC AGCCAGGCCA CAGTGAAGAA GCTACAGTCA TGTCTGCAGG 
     1981 CCTTGGAGCT AAATGTAGAC AAGAAAGAGT ACAGTCCCTC AGCCTTGCAG CTTGAGAACA 
     2041 TGGCGAAGGA GCTTGACACT GTGAGAGGCT CCTTGGGTAG GGAGAACCAG CTCCTGGCCA 
     2101 GCCTTTCAGA GCGCCTTGCT AGGGCAGAGA AAGGGGAAAA GACACCTCCA GACACAGAGC 
     2161 TTCATCAGGA GCCAGTTCCT GCGGATCTGG TGCTCAAGTT CCAGGAGCTG AAGGGAAAAC 
     2221 TTCAAGCCCT AGAAGGAGAG AACACCGAGG CCCAGGAGCT CAACAGGCAG CAGAGTATCA 
     2281 AGCTAGAGCA ACTGGCCAAG GAACTACAGC TGAAGGAGGA GGCCCGGGCT AGCCTAGCAC 
     2341 ACTTAGTGAA GGATGTGGTC CCACTTCAGG AAGAGCTGTC TGGAAAGAAG CAAGAGTCAG 
     2401 CTCAGCTCCG ACGACAGCTT CAGGAATCCC TGGCCCACTT GAGCTCTGTG GAGGAGGAGC 
     2461 TGGCTGAAGC CAGGCAGCAG GAAAAGCAGC ATCGAGAAGA AAAGCAGCTA CTGGAGCAGG 
     2521 AAGCCACGTC TCTTACATGG CAGCTGCAGC TCCTAGAGAC CCAGCTAGGA CAGGTGAGTC 
     2581 AGCTTGTAAG TGACCTGGAG GAGCAGAAGA AGCAACTCAT GCAGGAGAGA GACCATCTCA 
     2641 GCCAGAGAGT GGGCACACTG GAGCAGCTAG CCGAGGTGCA TGGCCCGCCA CAGTCTGCGG 
     2701 AGATGCCGGA AAAGAGGCAG CAGTGCCTCC GGGAAGAACA GGTAAACAAT AGCACAGTGT 
     2761 CAGAGGCAGA GCAGGAGGAG TTGCAGAAGG AGCTGCAGAA CATGGTTGAC CGCAACCAGC 
     2821 TCCTCGAAGG CAAACTGCAG GCCTTGCAAA CTGACTATAA GGCACTGCAG CAGCGAGAGG 
     2881 CAGCAATCCA GGGCTCTTTA GCCTCCCTGG AAGCTGAACA GGCTAGCATC CGGCACCTGG 
     2941 GAAACCAGAT GGAAGCAAGC CTCCTGGCTG TGAAGAAAGC CAAAGAGACT ATGAAAGCCC 
     3001 AGGTGGCAGA GAAGGAGGCT GCCCTGCAGA GTAAGGAGAG TGAGTGCCAG AGGCTGCAGG 
     3061 AAGAGGCAGA TCAGTGCCGG CTTCAGGCAG AGGCCCAGGC CCAGGAGCTC AGAGCTCTTG 
     3121 AGAACCAGTG CCAGCAGCAG ATTCAGCTGA TTGAGGTCCT CTCTGCAGAG AAAGGCCAAC 
     3181 AGGGACTCAG CCTGCCCCAA GTCAACACAG ACCAACTGGC CCTGTCTCAA GCACAGCTGG 
     3241 AAATCCACCA GGGGGAAGCC CAGCGGTTAC AGAATGAGGT GGTGGACCTC CAGGCCAAGC 
     3301 TCCAGGTGGC CCTAGGTGAT CGAGACAAGT TACAGAGCCA GCTGGGTGTG GCTGAGACAG 
     3361 TTCTAAGGGA GCACAAGACC CTGGTGCAAC AACTGAAAGA GCAGAATGAA GCCCTCAACA 
     3421 GGGCCCATGT TCAGGAGCTG CTTCAGTGCT CAGAGAGAGA GGGGATACTA CAGGAGGAGA 
     3481 GCATCTACAA GGCCCAGAAG CAGGAACAAG AGCTACGAGC CCTGCAGGCA GAGCTGTCAC 
     3541 AGGTGAGGTG CAGCTCTGAG GGAGCCCACC TGGAACATGC AGAGCTGCAA GACCAGCTGC 
     3601 ACCGAGCCAA CACAGACACA GCTGAGCTTG GTATACAGGT CTGTGCACTG ACAGCTGAGA 
     3661 AGGATCGAAT GGAGGAGGCC CTGGCCAGCC TAGCCCAGGA GCTCCAGGAC TCCAAAGAGG 
     3721 CAGCACTACA GGAACGAAAG GGCTTGGAGC TCCAAGTGAT GCAACTTCAG CAAGAGAAGG 
     3781 AGAAGTTGCA GGAAAAGGTG AAGGCAGCTG AGGAGGCAGC CAGTTCATTC TCTGGTCTGC 
     3841 AGGCACAGCT GGCCCAGGCT GAGCAGCTAG CCCAGAGCCT CCAAGAGACT GCACACCAGG 
     3901 AACAAGATGC CCTCAAGTTC CAACTAAGTG CTGAGATTAT GGACCACCAG AACCGATTAA 
     3961 AGACAGCCAA TGAAGAGTGT GGGCACCTCA GGGCCCAGCT AGAAGAACAA GGCCAGCAGC 
     4021 TGCAAATGAC TAAGGAGGCT GTGCAGGAAC TGGAGATCAC CAAGGCGGCC ATGGAGGAGA 
     4081 AGCTGAATTG CACCAGTAGC CACCTTGCAG AGTGCCAGGC CACTTTACTG CGCAAAGATG 
     4141 AAGAAAGCAC TATGCTTCAA ACCAGTCTAG AAAGAACACA GAAGGAACTT GAAAAGGCTA 
     4201 CATCAAAAAT TCAAGAATAT TACAACAAAC TCTGCCAAGA GGTGACAAAC AGGGAAAGGA 
     4261 ATGACCAGAA GATGCTTGCA GACCTCGATG ACCTGAACAG AACCAAGAAA TACCTTGAGG 
     4321 AGCGGCTGAT AGAGCTGCTC AGGGACAAAG ATGCTCTCTG GCAAAAATCC GATGCACTGG 
     4381 AAGAATTCCA GCAGAAGCTC AGTGCTGAAG AGAAATGTCT TGGGGACATG GAAGTCAACC 
     4441 ACTGCCATGA CTGCAAGCGG GAGTTCAGCT GGATAGTGCG ACGGCACCAC TGCAGGATAT 
     4501 GTGGCCGTAT CTTCTGTTAC TACTGCTGTA ATAACTATGT TGTGACTAAG CCCAGTGGCA 
     4561 AGAAGGAACG CTGCTGCCGA GCCTGCTTCC AGAAGTTCGG CGAAGGCTCT GGATCCAATG 
     4621 ATAGCAGTGG TTCAGGCACT AGCCAGGGAG AGCCCAGCCC CATGGTGTCA CCAGCTGAAG 
     4681 CAAGTCCCCA GTCCATAGGA AGCCAAGGGA TAAACTCAGT CTGCAGACCA CCAGACGATG 
     4741 CTGTGTTTGA CATCATCACT GATGAAGAAC TGTGCCAGAT CCAAGAATCT GGCTCCTCCT 
     4801 TGCCTGAAAC ACCCACTGAA ACTGATTCAA TGGACCCGAA TACGGCTGAA CAGGACACCA 
     4861 CATCAAACTC ATTAACCCCT GAAGACACTG AAGACGTGCC CATGGGGCAA GATGCTGAAA 
     4921 TCTGCTTGCT GAAGTCAGGA GAGCTGATGA TCAAATTACC CCTCACAGTA GAGGAGGTCG 
     4981 CCAGCTTCGG GGAGGGCAGC AGGGAGCTGT TTGTGAGGTC CAGTACCTAC AGCCTGATCA 
     5041 CCATCACCGT GGCTGAGCCT GGCCTCACCA TCAGCTGGGT CTTCTCTTCT GATCCCAAGA 
     5101 GCATCTCCTT CAGTGTGGTC TTCCAAGAGA CAGAAGACAC GCCGCTCGAC CAGTGCAAGG 
     5161 TCCTCATTCC CACCACCCGA TGCAATTCCC ACAAGGAGAA CATCCGGGGC CAGCTGAAGG 
     5221 TCCGAATCCC TGGCATCTAC TTGCTCATCT TTGACAACAC CTTTTCAAGA TTTATCTCCA 
     5281 AAAAAGTGCT TTACCACCTG ACTGTTGACC GGCCTGTGAT CTACGATGGA AGCGATTTCC 
     5341 CAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5401 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5461 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5521 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5581 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5641 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5701 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5761 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5821 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5881 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5941 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6001 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6061 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6121 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6181 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6241 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6301 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6361 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6421 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6481 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6541 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6601 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6661 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6721 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6781 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6841 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6901 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6961 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7021 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7081 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7141 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7201 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7261 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7321 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7381 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7441 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7501 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7561 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7621 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7681 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7741 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7801 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7861 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7921 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7981 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8041 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8101 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8161 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8221 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8281 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8341 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8401 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8461 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8521 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8581 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8641 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8701 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8761 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8821 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8881 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8941 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9001 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9061 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9121 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9181 TTTAACGCGA ATTTT
//