LOCUS       MR217638                8418 bp    DNA     circular  SYN 
DEFINITION  Ptpn21 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 21 (Ptpn21), transcript variant 1
ACCESSION   NM_011877
VERSION     NM_011877.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4573..4664 
                     /label="Myc-DDK"  
     misc_feature    4694..5279 
                     /label="PolyA signal"  
     misc_feature    4751..4770 
                     /label="Restriction Enzyme"  
     misc_feature    5555..6227 
                     /label="ColE1"  
     misc_feature    6657..7451 
                     /label="Kan/Neo"  
     misc_feature    7486..7788 
                     /label="SV40 ori"  
     misc_feature    7874..8351 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4548..4555 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4548 
                     /label="Ptpn21"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACTGCCA TTTGGATTGA AGTTGAAACG CACCCGACGC TACACTGTGT 
     1081 CCAGCAAGAG CTGCCTGGTT GCCCGGATCC AGCTGCTCAA TAATGAGTTT GTGGAGTTCA 
     1141 CTCTGTCTGT GGAGAGCACT GGCCAGGAGA GCCTAGAAGC TGTGGCCCAG CGCCTGGAGC 
     1201 TGAGAGAGGT CACTTACTTT AGCCTCTGGT ACTACAACAA GCAGAATCAG CGGCGCTGGG 
     1261 TGGATTTGGA GAAACCCCTG AAGAAGCAGC TGGACAAGCA CGCACTGGAG CCTACCGTCT 
     1321 ACTTCGGCGT GCTATTCTAT GTGCCTTCTG TTTCCCAGCT GCAGCAGGAG ATTACCAGGT 
     1381 ATCAGTATTA CCTGCAGCTG AAGAAAGATA TTCTGGAAGG AAACCTTCCC TGTACATTAG 
     1441 AACACGCCAT TCAGCTGGCC GGCTTAGCTG TTCAAGCTGA TTTTGGTGAC TTTGATCAGT 
     1501 ATGAATCCCA AGATTTTCTT CAGAAATTTG CCTTGCTCCC CGTGGCATGG CTACAGGATG 
     1561 AAAAAGTGCT GGAAGAAGCA GCCCAGAAAG TGGCCTTACT GCACCAGAAA TACAGAGGGC 
     1621 TCACAGCTCC TGAGGCCGAG CTGCTGTACA TGCAGGAGGT AGAGAGAATG GATGGCTACG 
     1681 GAGAGGAGAG CTACCCTGCC AAGGACAGCC AAGGAAGTGA CATATCCATT GGTGCCTGTC 
     1741 TCGATGGCAT CTTTGTGAAA CACAAGAACG GAAGGCCTCC TGTGGTGTTC AGGTGGCACG 
     1801 ACATTGCTAA CATGTCGCAT AACAAGTCCT TTTTTGCACT AGAGCTGGCA AATAAAGAGG 
     1861 AGACCATCCA GTTTCAAACT GAAGACATGG AGACAGCCAA GTACGTGTGG CGGCTGTGTG 
     1921 TGGCACGGCA CAAATTCTAC AGACTGAACC AGTGTAGCCT GCAAACGCAG GCCGCCACGC 
     1981 TGAACTCAGT CAGGAGGGGC TCATCCTCCA GGATGTCTCT GCCTAAACCC CAGCCCTATG 
     2041 CGATGCCTCC CCCACCCCAG CTGCATTATA ATGGACATTA TACAGAGCCA TTTGCTTCTT 
     2101 CCCAAGACAA CATCTTTGTG CCCAACAAGA ATGGATTCTA CTGTCACTCC CAGACAAGCC 
     2161 TGGATAGAAC CCAGATTGAC CTCAGTGGTC GCATCCGCAA CGGCAGCGTC TACAGTGCAC 
     2221 ACAGTACGAA TTCCTTAAAC ACACTTCAGC CCTACCTGCA GCCCTCCCCC ATGTCCTCCA 
     2281 ACCCGAGTAT CACTGGCAGT GATGTCATGA GACCCGACTA CATCCCGTCC CACCGGCACA 
     2341 GTGCGCTCAT TCCCCCGTCT TACCGCCCGA CCCCTGACTA CGAGACCGTG ATGAAGCAGC 
     2401 TCAACAGAGG CATGGTGCAC GCAGACCGGC ACAGCCACTC GCTGAGGAAC CTCAACATTG 
     2461 GCAGTTCCTA TGCGTACAGC CGGCCCGACG CCTTGGTCTA CAGCCAGCCC GAGATTCGGG 
     2521 AGCACCCTCA CCTCACCTCT CCCCAGTCAG CCCACTACCC CTTTAACCTG AACTACAGTT 
     2581 TCCACAGCCA GTCTCCGTAT CCCTACCCTG CCGAGAGGCG GCCAGTGGTG GGCGCCGTGA 
     2641 GTGTGCCTGA ACTGACAAAT GTGCAGCTGC AGGCTCAGGA CTACCCAGCT CCAAACATTA 
     2701 TGAGAACCCA GGTGTACCGC CCACCCCCGC CGTATCCTTA CCCAAGACCT GCCAACAGCA 
     2761 CCCCAGACCT GTCCCGCCAC CTCTACATCA GCAGCAGCAA TCCGGATCTC ATCACCAGGC 
     2821 GCGTCCATCA CTCAGTGCAG ACCTTCCAGG AGGACAGCTT GCCCGTGGCT CACTCTCTGC 
     2881 AGGAGGTCAG CGAGCCCCTC ACAGCAGCGC GCCATGCCCA CCTACAGAAG AGGAACAGTA 
     2941 TTGAAATCGC AGGGCTCACA CATGGATTCG AAGGCCTGAG GCTCAAGGAG AGGACGGTGT 
     3001 CAGCCTCAGC AGCAGACGTG GCGCCCCGGA CCTTCTCAGC AGGCTCCCAG TCCAGCGTCT 
     3061 TCTCTGACAA AATGAAGCAG GAGGGGACTG AAGAGCAGGA GGGTGGCAGA TACAGCCATA 
     3121 AGAAGTCCCT TTCTGATGCC ACCATGCTGA TTCATAGCAG TGAGGAGGAC GAGGACTTGG 
     3181 AAGAGGACAG CAGCAGAGAA CAGGCCATCT CAGCTGTGTC TGAGCCCCGC CTTACGGCCG 
     3241 CTTTCTCTCA GGAACTAAAC TACCCCTGTG CTTCAGCGAC TCCGATCACT GGGCCTCTGC 
     3301 ATATTTTTGA GCCCAAGCCC CATGTCACAG AGCCTGAGAA GAGGGCAAAG GACATCAGCC 
     3361 CTGCCCACCT GGTTGTGGAG ACCCATCGGC CCCGAAGAGA TGGACTGCTG ACCCCCTCCA 
     3421 TGTCCGAGTC AGACCTCACG ACCTCAGGGA GGTACCGAGC TAGGAGGGAC TCTGTCAAGA 
     3481 AAAGGCCAGT GTCAGACCTC CTCTCTGGGA AAAAAAGCGC TGTGGAAGGA CTTCCGCCAC 
     3541 TAGGGGGGAT GAAAAAAACT CGAGCAGATG CAAAAAAAAT TGGTCCCCTC AAGCTGGCAG 
     3601 CCCTCAATGG GCTCTCCCTG TCCCGGCTGC CCCTGCCTGA TGAAGGAAAA GAAGTGTCCA 
     3661 CCAGAGCGAC AAATGACGAG AGGTGCAAGG TTCTGGAGCA GCGGTTAGAG CAAGGCATGG 
     3721 TCTTCACCGA ATATGAGAGG ATCCTTAAGA AGCGGCTGGT TGATGGTGAG TGCTCGACTG 
     3781 CCCGGCTCCC TGAAAATGCA GAGAGAAATC GATTTCAAGA TGTGCTCCCC TACGATGATG 
     3841 CGAGAGTCGA GTTGGTGCCA ACCAAGGAGA ACAACACTGG CTACATCAAC GCGTCACACA 
     3901 TTAAGGTCTC TGTCAGCGGA ATAGAATGGG ATTATATTGC CACACAGGGA CCATTACAAA 
     3961 ATACCTGCCA GGACTTTTGG CAGATGGTGT GGGAACAGGG AGTCGCAATT ATAGCAATGG 
     4021 TGACAGCAGA AGAGGAGGGA GGCCGGGAGA AGAGCTTTAG GTACTGGCCA CGGCTTGGTT 
     4081 CCAGGCACAA CACTGTCACC TATGGAAGGT TTAAGATCAC AACGCGCTTC CGCACGGACT 
     4141 CGGGCTGCTA TGCCACCACA GGTTTGAAGA TGAAGCACCT TCTCACAGGG CAGGAGAGAA 
     4201 CCGTTTGGCA TCTTCAGTAC ACAGACTGGC CTGAACACGG CTGTCCCGAA GACCTCAAAG 
     4261 GATTTTTATC CTACCTTGAA GAGATCCAGT CAGTTCGACG TCATACAAAC AGTACCAGTG 
     4321 AACCCAAAAG CCACAATCCC CCGCTGCTGG TGCACTGCAG TGCTGGCGTG GGAAGAACTG 
     4381 GGGTCGTCAT TCTGTCAGAG ATCATGGTTG CCTGTCTGGA ACACAATGAG GTGCTGGACA 
     4441 TCCCCAGAGT GCTGGACATG CTGAGGCAGC AGAGGATGAT GCTGGTGCAG ACACTTGGCC 
     4501 AGTACACCTT TGTGTACAGA GTGCTCATTC AGTTCCTGAA AAGCTCCAGG CTCATTAGCG 
     4561 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4621 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4681 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4741 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4801 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4861 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4921 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4981 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5041 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5101 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5161 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5221 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5281 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5341 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5401 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5461 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5521 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5581 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5641 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5701 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5761 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5821 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5881 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5941 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6001 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6061 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6121 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6181 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6241 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6301 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6361 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6421 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6481 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6541 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6601 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6661 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6721 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6781 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6841 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6901 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6961 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7021 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7081 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7141 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7201 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7261 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7321 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7381 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7441 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7501 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7561 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7621 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7861 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7921 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7981 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8041 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8101 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8161 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8221 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8281 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8341 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8401 AAATTTAACG CGAATTTT
//