LOCUS       MR217612                8253 bp    DNA     circular  SYN 
DEFINITION  Sh3pxd2a (Myc-DDK-tagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a), transcript variant 1
ACCESSION   NM_008018
VERSION     NM_008018.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4410..4501 
                     /label="Myc-DDK"  
     misc_feature    4531..5116 
                     /label="PolyA signal"  
     misc_feature    4588..4607 
                     /label="Restriction Enzyme"  
     misc_feature    5392..6064 
                     /label="ColE1"  
     misc_feature    6494..7288 
                     /label="Kan/Neo"  
     misc_feature    7323..7625 
                     /label="SV40 ori"  
     misc_feature    7711..8188 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4392..4398 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4392 
                     /label="Sh3pxd2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCGCCTAC TGCGTGCAAG ATGCCACCGT GGTGGACGTG GAGAAGCGGA 
     1081 GGAGCCCCTC TAAACACTAT GTATACATTA TCAACGTGAC CTGGTCTGAC TCCACCTCCC 
     1141 AGACTATCTA CCGGAGGTAC AGCAAGTTCT TCGACCTGCA GATGCAGCTT CTGGATAAGT 
     1201 TTCCTATTGA AGGTGGCCAG AAGGATCCGA AGCAAAGGAT TATTCCCTTT CTTCCAGGCA 
     1261 AGATCCTCTT CCGGAGAAGC CACATCCGCG ACGTGGCTGT GAAGAGACTA AAGCCCATCG 
     1321 ATGAATACTG CAGGGCGCTT GTCCGGCTGC CGCCCCACAT TTCACAGTGT GACGAAGTCT 
     1381 TCCGGTTCTT TGAGGCACGG CCTGAGGATG TCAACCCTCC AAAAGAAGAC TATGGCAGTT 
     1441 CCAAGAGGAA ATCAGTGTGG TTGTCCAGCT GGGCTGAGTC TCCCAAGAAG GACGTGACAG 
     1501 GTGCCGACAC CAACGCCGAG CCCATGATCC TGGAACAGTA CGTGGTGGTG TCCAACTATA 
     1561 AGAAACAAGA GAACTCGGAG CTGAGCCTCC AGGCCGGGGA GGTGGTAGAT GTCATCGAGA 
     1621 AGAACGAAAG CGGCTGGTGG TTTGTGAGCA CATCTGAAGA GCAAGGTTGG GTCCCCGCCA 
     1681 CCTACTTGGA GGCCCAGAAT GGCACACGAG ACGACTCGGA CATCAACACC TCCAAGACTG 
     1741 GGGAAGTGTC CAAGAGACGC AAGGCACACC TGCGGCGCCT GGATCGCCGG TGGACCCTGG 
     1801 GCGGGATGGT CAACAGGCAG CACAGCCGAG AAGAGAAGTA TGTCACTGTG CAGCCCTACA 
     1861 CCAGCCAGAG CAAAGACGAG ATCGGCTTCG AGAAGGGTGT CACCGTGGAG GTGATTCGAA 
     1921 AGAACCTGGA AGGCTGGTGG TACATCAGGT ACCTCGGCAA AGAGGGTTGG GCACCAGCGT 
     1981 CCTACCTGAA GAAGGCCAAG GATGACCTGC CGACCCGGAA GAAGAACCTG GCGGGTCCGG 
     2041 TGGAGATCAT AGGGAACATT ATGGAGATCA GCAACCTTCT CAACAAGAAG GCATCTGGGG 
     2101 ATAAGGAGGC TCCGGCTGAA GGCGAGGGAT CCGAGGCCCC CATCACCAAG AAAGAGATCA 
     2161 GCTTACCGAT CCTCTGCAAC GCCTCCAATG GCAGCGCCTT GGCCATTCCC GAGAGGACCA 
     2221 CATCCAAGCT AGCCCAGGGC TCCCCAGCTG TGGCCAGGAT CGCCCCTCAG AGGGCCCAGA 
     2281 TCAGCTCCCC AAATCTGAGG ACAAGACCTC CCCCGCGCAG AGAATCCAGC CTGGGGTTCC 
     2341 AGCTGCCAAA GCCGCCAGAG CCCCCTTCTG TTGAGGTAGA ATACTACACC ATTGCTGAAT 
     2401 TCCAGTCCTG CATTTCTGAC GGGATCAGCT TTCGAGGCGG ACAGAAGGCA GAGGTCATCG 
     2461 ACAAGAACTC GGGTGGTTGG TGGTACGTGC AGATCGGGGA GAAGGAGGGC TGGGCCCCAG 
     2521 CCTCATACAT TGACAAGCGC AAGAAACCCA ACCTCAGCCG CCGAACCAGC ACTCTGACGC 
     2581 GGCCCAAGGT GCCGCCACCT GCGCCCCCCA GCAAGCCTAA GGAGGCCGAG GAGAATCCTG 
     2641 TGGGTGCCTG TGAGAGCCAG GGCTCCCCAC TGAAGGTTAA ATACGAGGAA CCCGAGTATG 
     2701 ACGTCCCTGC CTTTGGCTTT GACTCAGAGC CCGAGATGAA TGAAGAGCCT TCAGGGGACA 
     2761 GAGGTTCAGG TGACAAGCAT CCCGCCCAGC CCCGAAGGAT CTCGCCTGCC TCTTCCCTGC 
     2821 AACGGGCCCA TTTCAAGGTG GGTGAGTCTT CTGAGGACGT GGCCCTGGAA GAGGAGACCA 
     2881 TCTATGAGAA TGAGGGCTTC AGGCCATACA CAGAAGACAC CCTGTCTGCC AGAGGCTCCT 
     2941 CTGGGGACAG TGACTCCCCT GGGAGCTCCT CTTTGTCCCT TGCCGTGAAA AACTCCCCTA 
     3001 AATCAGATTC CCCCAAATCC TCATCACTCC TAAAGCTCAA AGCAGAGAAG AATGCCCAGG 
     3061 CAGAACTGGG GAAAAACCAG TCCAACATCT CCTTCTCCTC CTCTGTCACC ATCAGCACCA 
     3121 CCTGTTCCTC CTCCTCATCC TCGTCCTCCT TGTCCAAGAA CAATGGTGAC CTGAAACCAC 
     3181 GTTCTGCCTC AGATGCAGGT ATCCGTGACA CCCCTAAGGT TGGGACCAAG AAAGATCCTG 
     3241 ATGTGAAGGC CGGGCTGGCC TCCTGCGCCC GAGCCAAGCC ATCCGTGAGA CCAAAGCCAG 
     3301 TCCTGAACCG AGCGGAGTCT CAAAGCCAGG AGAAGATGGA TATTAGTTCC TTACGGCGCC 
     3361 AGCTGAGGCC CACAGGCCAG CTCCGGGGGG GCCTCAAGGG CTCTAGGAGT GAGGACTCAG 
     3421 AGCTGCCTCC ACAGATGGCT TCTGAGGGAT CCAGGCGAGG TTCTGCGGAC ATCATCCCTC 
     3481 TCACGGCCAC CACTCCCCCG TGTGTCCCCA AAAAGGAATG GGAAGGGCAA GGCGCCACCT 
     3541 ACGTGACGTG CAGCGCCTAT CAGAAGGTCC AGGACTCGGA GATCAGCTTC CCCGAAGGCG 
     3601 CCGAGGTGCA CGTGCTGGAG AAGGCGGAAA GTGGGTGGTG GTACGTGAGG TTTGGGGAGC 
     3661 TGGAGGGCTG GGCTCCTTCC CACTACTTGG TGGCCGAGGA GAACCAGCAA CCTGACACAG 
     3721 CTAGCAAAGA GGGAGACACA GGAAAGAGCT CGCAGAACGA GGGCAAGTCA GACAGCCTGG 
     3781 AAAAGATTGA GAAGCGTGTG CAGGCGCTCA ACACTGTGAA CCAGAGCAAG AGGGCCACCC 
     3841 CACCCATCCC CTCGAAGCCT CCCGGGGGCT TCGGCAAGAC CTCGGGCACC GTAGCGGTGA 
     3901 AGATGAGGAA CGGGGTCCGG CAAGTGGCCG TCAGGCCCCA ATCTGTGTTT GTGTCTCCGC 
     3961 CACCCAAGGA CAACAACCTG TCCTGTGCCC TTCGGAGGAA CGAGTCGCTA ACGGCCACCG 
     4021 ACAGCCTCAG AGGTGTCCGC AGGAACTCCT CCTTTAGCAC CGCACGGTCA GCAGCCGCTG 
     4081 AGGCCAAGGG CCGCCTGGCC GAGCGGGCTG CCAGCCAGGG CTCAGAATCG CCCCTGCTGC 
     4141 CTACCCAGCG CAAAGGCATC CCTGTCTCCC CCGTGCGTCC CAAGCCCATA GAGAAGTCCC 
     4201 AGTTTATCCA CAACAACCTC AAGGATGTGT ACATCTCGAT TGCAGACTAT GAGGGGGACG 
     4261 AAGAGACGGC TGGCTTCCAG GAGGGGGTGT CCATGGAGGT GCTGGAGAAG AACCCCAATG 
     4321 GCTGGTGGTA CTGCCAGATC CTGGATGAGG TGAAGCCCTT CAAGGGCTGG GTACCCTCCA 
     4381 ACTACCTTGA GAAGAAGAAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4441 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4501 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4561 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4621 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4681 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4741 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4801 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4861 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4921 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4981 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5041 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5101 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5161 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5221 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5281 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5341 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5401 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5461 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5521 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5581 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5641 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5701 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5761 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5821 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5881 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5941 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6001 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6061 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6121 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6181 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6241 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6301 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6361 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6421 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6481 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6541 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6601 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6661 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6721 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6781 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6841 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6901 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6961 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7021 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7081 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7141 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7201 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7261 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7321 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7381 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7441 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7501 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7681 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7741 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7801 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7861 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7921 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7981 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8041 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8101 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8161 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8221 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//