LOCUS       MR217558                7986 bp    DNA     circular  SYN 
DEFINITION  Atp12a (Myc-DDK-tagged) - Mouse ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a)
ACCESSION   NM_138652
VERSION     NM_138652.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4143..4234 
                     /label="Myc-DDK"  
     misc_feature    4264..4849 
                     /label="PolyA signal"  
     misc_feature    4321..4340 
                     /label="Restriction Enzyme"  
     misc_feature    5125..5797 
                     /label="ColE1"  
     misc_feature    6227..7021 
                     /label="Kan/Neo"  
     misc_feature    7056..7358 
                     /label="SV40 ori"  
     misc_feature    7444..7921 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4125..4131 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4125 
                     /label="Atp12a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCCGGAAA ACAGAAATCT ATTCTGTAGA GCTCAATGGA ACTAAAGACG 
     1081 TCGAGCTGGC AGACCAGAAA GATGACAAAA AGTTCAAGGG CGGGAAGAAC AAAGACTCAG 
     1141 AACCCAACAA GAGTCAGGAG GAGGAGCTGA AGAAAGAACT TGATCTGGAT GACCACAGAC 
     1201 TCAGTAATAC AGATTTGGAA CAGAAATATG GTACAAACAT CATCCAGGGT CTCTCCAGTA 
     1261 TCAGAGCTGC AGAGCTCCTG GCTCGGGATG GGCCCAACGC CCTCACCCCT CCCAAGCAAA 
     1321 CTCCAGAGAT CATCAAGTTC CTCAAACAGA TGGTGGGGGG CTTTTCCATC CTTCTGTGGA 
     1381 TAGGTGCAGC CTTGTGCTGG ATCGCATATG TGATCCAGTA TGTCAGCAGT ACTGCATCCC 
     1441 TGGACAATGT CTATTTGGGC GCCATACTTG TCCTGGTCGT TATTTTAACG GGGATTTTTG 
     1501 CTTATTACCA AGAAGCCAAA AGTACCAACA TTATGGCAAG CTTTAGTAAG ATGATCCCCC 
     1561 AGCAAGCTCT TGTCATCCGA GATGCAGAAA AGAAGATCAT TCCAGCAGAG CAGCTGGTGG 
     1621 TGGGGGACGT TGTGGAGATT AAAGGAGGGG ACCAGATCCC TGCAGACATC AGACTGGTGT 
     1681 TTTCCCAAGG GTGCAAAGTA GATAACTCCT CTCTCACTGG GGAGTCTGAG CCCCAGGCTC 
     1741 GGTCAACTGA GTTTACCCAT GAAAACCCCT TGGAAACTAA GAACATAGGC TTCTATTCTA 
     1801 CAACGTGCCT GGAAGGTACG GCAACTGGCA TTGTCATCAA CACGGGTGAC CGCACCATCA 
     1861 TCGGGCGCAT AGCCTCCTTG GCTTCAGGCG TTGGGAGTGA GAAGACACCC ATAGCCATTG 
     1921 AGATTGAGCA TTTTGTCCAT ATTGTGGCAG CAGTGGCTGT GTCCGTTGGC GTCATTTTCT 
     1981 TCATCACCGC CGTGTGCATG AAGTACTACG TCCTGGATGC CATCATCTTC CTCATTAGCA 
     2041 TCATTGTGGC CAATGTGCCA GAGGGCCTCC TGGCTACTGT CACTGTTACC CTGTCACTGA 
     2101 CGGCAAAGCG GATGGCTAAG AAGAACTGCT TGGTAAAGAA CCTGGAGGCC GTGGAGACGC 
     2161 TTGGCTCCAC CTCCATCATC TGCTCAGATA AGACAGGGAC CCTCACCCAG AACAGGATGA 
     2221 CTGTGGCCCA CTTGTGGTTT GACAACCAGA TCTTTGTGGC TGATACAAGT GAAAACCAGA 
     2281 CAAAACAAGC CTTTGACCAA AGCTCTGGAA CCTGGGCTTC CTTGTCCAAG ATAATAACAC 
     2341 TGTGTAACCG AGCAGAGTTC AGGCCAGGCC AGGAGAGTGT CCCCATCATG AAGAGAGTTG 
     2401 TGGTTGGAGA TGCCTCAGAA ACTGCTCTGC TAAAGTTCTC AGAGGTTATT TTGGGTGATG 
     2461 TGATGGACAT TAGGAAAAGA AACCACAAAG TAGCTGAGAT TCCTTTTAAT TCTACCAACA 
     2521 AATTTCAGCT CTCCATACAT GAGACCGAGG ACCCGAATGA CAAGCGCTTC CTCATGGTGA 
     2581 TGAAAGGGGC CCCTGAGAGG ATCTTAGAGA AGTGCAGCAC CATCATGATC AACGGCCAGG 
     2641 AGCAGCCACT GGACAAGAGC TCTGCCGATG CCTTCCACAC GGCCTACATG GAGCTGGGTG 
     2701 GTCTGGGAGA GCGTGTGTTG GGTTTCTGTC ACCTCTACTT GCCAGCAGAT AAGTTTCCCC 
     2761 AAAGCTACAC ATTTGATGTA GACTCCATAA ACTTTCCCAC TTCCAATCTC TGCTTTGTGG 
     2821 GGCTTCTGTC CATGATTGAC CCTCCTCGGT CTACCGTGCC AGATGCGGTC TCCAAATGTC 
     2881 GGAGTGCGGG GATCAAGGTT ATCATGGTCA CAGGTGATCA CCCTATCACA GCCAAAGCTA 
     2941 TTGCCAAGAG TGTAGGAATC ATATCAGCCA ACAATGAGAC GGTGGAAGAC ATCGCAAAAC 
     3001 GCCGCAACAT TGCTGTGGAG CAAGTCAACA AAAGAGAAGC GAAAGCCGCT GTGGTGACTG 
     3061 GCATGGAACT GAAGGACATG ACCCCAGAAC AACTGGATGA GCTTCTAATC AACTACCAAG 
     3121 AAATTGTGTT TGCCCGGACA TCACCCCAGC AGAAGTTGAT CATTGTAGAA GGCTGTCAGA 
     3181 GGCAGGATGC AGTTGTTGCT GTGACGGGTG ATGGAGTAAA CGACTCTCCA GCATTAAAGA 
     3241 AGGCAGACAT CGGGATTGCT ATGGGGATAG CAGGTTCTGA TGCTGCTAAG AATGCAGCTG 
     3301 ACATGGTCTT GCTCGATGAC AACTTTGCAT CTATAGTCAC AGGGGTGGAG GAAGGTCGCC 
     3361 TGATCTTCGA CAACCTGAAG AAGACCATTG CTTACACCCT GACAAAGAAC ATCGCTGAGC 
     3421 TCTGCCCTTT TTTGATCTAC ATTGTTGCTG GGCTCCCCCT GCCTATTGGC ACCATCACTA 
     3481 TCCTATTCAT CGACCTGGGC ACAGACATTA TCCCCTCCAT TGCCTTGGCC TATGAGAAAG 
     3541 CGGAAAGTGA CATTATGAAT AGGAAACCTC GCCACAAGAA GAAAGACAGA CTGGTGAACA 
     3601 AGCAGCTGGC CATCTACTCT TACCTTCACA TTGGTCTCAT GCAAGCCCTG GGAGGTTTCC 
     3661 TCGTCTATTT CACTGTCTAT GCACAGCAGG GCTTTTGGCC CACCTCTCTC ATCAACCTGA 
     3721 GGGTATCATG GGAAACAGAT GACATAAATG ACTTGGAAGA TAGCTACGGG CAGGAATGGA 
     3781 CGAGGTATCA GAGAAAATAC CTGGAATGGA CAGGCTCAAC GGCTTTCTTT GTTGCCATCA 
     3841 TGGTCCAACA AATTGCAGAT CTGATCATCA GGAAAACCCG GAGGAATTCC ATCTTCCAGC 
     3901 AGGGGCTCTT CAGAAACAAA GTCATCTGGG TGGGGATCAT CTCGCAGATC ATCGTTGCGT 
     3961 TGGTCCTCTC CTACGGTCTC GGGAGTGTCA CAGCTCTGAG CTTTACCATG CTCAGGGCTC 
     4021 AGTACTGGTT TGTGGCTGTC CCGCATGCCA TTTTGATTTG GGTGTATGAC GAGATGCGGA 
     4081 AGTTGTTCAT CAGGCTCTAC CCTGGAAGCT GGTGGGACAA GAACATGTAT TATACGCGTA 
     4141 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4201 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4261 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4321 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4381 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4441 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4501 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4561 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4621 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4681 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4741 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4801 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4861 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4921 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4981 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5041 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5101 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5161 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5221 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5281 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5341 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5401 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5461 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5521 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5581 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5641 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5701 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5761 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5821 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5881 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5941 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6001 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6061 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6121 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6181 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6241 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6301 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6361 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6421 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6481 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6541 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6601 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6661 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6721 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6781 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6841 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6901 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6961 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7021 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7081 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7141 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7201 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7261 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7321 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7381 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7441 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7501 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7561 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7621 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7681 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7741 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7801 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7861 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7921 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7981 AATTTT
//