LOCUS       MR217545                8196 bp    DNA     circular  SYN 
DEFINITION  Pold1 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1)
ACCESSION   NM_011131
VERSION     NM_011131.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4353..4444 
                     /label="Myc-DDK"  
     misc_feature    4474..5059 
                     /label="PolyA signal"  
     misc_feature    4531..4550 
                     /label="Restriction Enzyme"  
     misc_feature    5335..6007 
                     /label="ColE1"  
     misc_feature    6437..7231 
                     /label="Kan/Neo"  
     misc_feature    7266..7568 
                     /label="SV40 ori"  
     misc_feature    7654..8131 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4335..4341 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4335 
                     /label="Pold1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTGTAAG CGGCGACAAG GACCAGGCCC TGGGGTGCCC CCAAAGCGGG 
     1081 CTCGAGGGCA CCTCTGGGAT GAGGACGAGC CTTCGCCGTC GCAGTTTGAG GCGAACCTGG 
     1141 CACTGCTGGA GGAAATAGAG GCTGAGAACC GGCTGCAGGA GGCAGAGGAG GAGCTGCAGC 
     1201 TGCCCCCAGA GGGCACCGTG GGTGGGCAGT TTTCCACTGC AGACATTGAC CCTCGGTGGC 
     1261 GGCGGCCCAC CCTACGTGCC CTGGACCCCA GCACGGAGCC CCTCATCTTC CAGCAGCTGG 
     1321 AGATTGACCA CTATGTGGGC TCAGCACCAC CCCTGCCAGA AGGGCCCCTG CCATCCCGGA 
     1381 ACTCAGTGCC CATACTGAGG GCCTTTGGGG TCACCGATGA AGGCTTCTCC GTCTGCTGCC 
     1441 ACATACAGGG CTTTGCCCCC TACTTCTACA CCCCCGCGCC TCCTGGTTTT GGGGCCGAGC 
     1501 ACCTGAGTGA GCTGCAGCAG GAGCTGAACG CAGCCATCAG CCGGGACCAG CGCGGTGGGA 
     1561 AGGAGCTCTC AGGGCCGGCA GTGCTGGCAA TAGAGCTATG CTCCCGTGAG AGCATGTTTG 
     1621 GGTACCACGG TCATGGCCCT TCTCCATTTC TCCGCATCAC CCTGGCACTA CCCCGCCTTA 
     1681 TGGCACCAGC CCGCCGCCTT CTGGAACAGG GTGTCCGAGT GCCAGGCCTG GGCACCCCGA 
     1741 GCTTCGCACC CTACGAAGCC AACGTGGACT TTGAGATCCG GTTCATGGTG GATGCTGACA 
     1801 TTGTGGGATG CAACTGGTTG GAGCTGCCAG CTGGAAAGTA CGTTCGGAGG GCGGAGAAGA 
     1861 AGGCCACCCT GTGTCAGCTG GAGGTGGACG TGCTGTGGTC AGATGTGATC AGTCACCCAC 
     1921 CGGAGGGGCA GTGGCAGCGC ATTGCACCCC TGCGTGTGCT TAGCTTCGAC ATCGAGTGTG 
     1981 CTGGCCGAAA AGGCATCTTC CCTGAGCCTG AGCGTGACCC CGTGATCCAG ATCTGTTCTC 
     2041 TGGGGCTGCG CTGGGGGGAG CCGGAGCCAT TCTTGCGTCT GGCACTCACG CTGCGGCCCT 
     2101 GTGCCCCCAT CCTGGGTGCC AAAGTGCAGA GCTATGAGCG GGAAGAAGAC CTGCTCCAGG 
     2161 CCTGGGCCGA CTTCATCCTT GCCATGGACC CTGACGTGAT CACCGGCTAC AACATTCAGA 
     2221 ACTTTGACCT CCCATACCTC ATCTCTCGGG CACAGGCCCT AAAGGTGGAC CGCTTCCCTT 
     2281 TCCTGGGCCG CGTGACTGGT CTCCGCTCCA ACATCCGTGA CTCCTCCTTC CAATCAAGGC 
     2341 AGGTCGGCCG GCGGGACAGT AAGGTGATCA GCATGGTGGG TCGCGTTCAG ATGGATATGC 
     2401 TGCAGGTGCT GCTTCGGGAA CACAAGCTCC GCTCCTACAC GCTCAACGCT GTGAGTTTCC 
     2461 ACTTCCTGGG CGAGCAGAAG GAGGACGTTC AGCACAGCAT CATCACCGAC CTGCAGAATG 
     2521 GGAACGAACA GACGCGCCGC CGCCTGGCCG TGTACTGCCT GAAGGACGCC TTTCTGCCAC 
     2581 TCCGACTACT AGAGCGCCTT ATGGTGCTGG TGAATAATGT GGAGATGGCG CGTGTCACGG 
     2641 GTGTACCCCT TGGGTACCTG CTCACCCGGG GCCAGCAGGT CAAGGTCGTG TCTCAGCTGC 
     2701 TGCGCCAGGC CATGCGCCAG GGGCTGCTGA TGCCTGTGGT GAAGACCGAG GGCAGTGAGG 
     2761 ACTACACGGG AGCCACAGTC ATTGAGCCCC TCAAAGGGTA CTATGACGTC CCCATTGCCA 
     2821 CCCTGGACTT CTCCTCCTTG TACCCATCCA TCATGATGGC CCATAATCTG TGCTACACCA 
     2881 CGCTGCTCCG ACCTGGGGCT GCCCAGAAGC TGGGCCTTAA ACCAGATGAG TTCATCAAGA 
     2941 CACCCACTGG GGATGAGTTT GTGAAGTCAT CTGTACGGAA GGGCCTCCTG CCCCAGATCC 
     3001 TGGAGAATCT GCTGAGTGCC CGCAAGAGGG CCAAGGCTGA GCTGGCTCAG GAGACGGACC 
     3061 CCCTGCGGCG ACAGGTCTTG GACGGCCGGC AACTGGCACT AAAAGTGAGT GCCAACTCCG 
     3121 TATATGGCTT CACTGGTGCC CAGGTGGGCA AGCTGCCATG TTTGGAGATC TCCCAGAGTG 
     3181 TCACTGGGTT CGGGCGGCAG ATGATTGAGA AAACCAAGCA GCTTGTGGAG TCCAAGTACA 
     3241 CCGTGGAAAA TGGCTACGAT GCCAACGCCA AGGTAGTCTA CGGTGACACG GACTCTGTGA 
     3301 TGTGCCGGTT TGGCGTCTCC TCTGTGGCTG AAGCAATGTC TCTGGGGCGG GAGGCTGCAA 
     3361 ACTGGGTATC CAGTCACTTC CCATCACCCA TCCGGCTGGA GTTCGAGAAG GTTTACTTCC 
     3421 CATACCTGCT CATCAGCAAG AAGCGCTATG CTGGCCTGCT CTTCTCCTCC CGCTCTGATG 
     3481 CCCATGACAA AATGGACTGC AAGGGCCTGG AGGCTGTGCG CAGGGACAAC TGTCCCCTGG 
     3541 TGGCCAACCT CGTTACATCC TCTCTGCGCC GGATCCTCGT GGACCGGGAC CCTGATGGGG 
     3601 CAGTAGCCCA TGCCAAGGAC GTCATCTCGG ACCTGCTGTG CAACCGCATA GACATCTCCC 
     3661 AGCTGGTCAT CACCAAAGAG TTGACCCGCG CAGCAGCAGA CTATGCTGGC AAGCAGGCTC 
     3721 ACGTGGAGCT GGCTGAGAGG ATGAGGAAGC GCGACCCCGG CAGTGCGCCC AGCCTGGGTG 
     3781 ACCGAGTCCC CTATGTGATC ATTGGTGCTG CTAAGGGTGT GGCCGCCTAC ATGAAGTCGG 
     3841 AGGACCCCCT GTTTGTGCTG GAGCACAGCC TGCCCATCGA CACTCAGTAC TACCTGGAGC 
     3901 AGCAGCTGGC CAAGCCGCTC TTGCGCATCT TTGAGCCCAT CCTGGGTGAG GGCCGTGCAG 
     3961 AGTCTGTGCT GCTGCGCGGT GACCACACAC GATGCAAGAC TGTGCTCACC AGCAAGGTGG 
     4021 GCGGCCTCTT GGCCTTCACC AAGCGCCGCA ACTGTTGCAT TGGCTGCCGC TCCGTAATCG 
     4081 ACCATCAAGG AGCCGTGTGT AAGTTCTGTC AGCCACGGGA GTCGGAGCTC TATCAGAAGG 
     4141 AGGTGTCACA CCTGAATGCC TTGGAAGAAC GGTTCTCTCG CCTCTGGACA CAGTGTCAAC 
     4201 GCTGCCAGGG CAGCTTGCAT GAGGACGTCA TCTGTACCAG CCGTGACTGT CCCATCTTCT 
     4261 ACATGCGCAA GAAGGTGCGC AAGGACCTGG AAGACCAGGA ACGGCTGCTG CAGCGCTTTG 
     4321 GACCGCCCGG CCCTGAGGCC TGGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4381 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4441 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4501 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4561 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4621 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4681 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4741 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4801 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4861 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4921 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4981 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5041 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5101 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5161 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5221 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5281 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5341 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5401 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5461 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5521 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5581 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5641 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5701 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5761 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5821 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5881 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5941 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6001 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6061 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6121 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6181 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6241 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6301 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6361 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6421 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6481 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6541 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6601 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6661 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6721 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6781 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6841 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6901 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6961 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7021 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7081 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7141 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7201 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7261 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7321 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7381 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7441 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7501 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7561 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7621 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7681 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7741 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7801 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7861 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7921 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7981 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8041 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8101 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8161 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//