LOCUS       MR217530                8841 bp    DNA     circular  SYN 
DEFINITION  Col20a1 (Myc-DDK-tagged) - Mouse collagen, type XX, alpha 1 (Col20a1)
ACCESSION   NM_028518
VERSION     NM_028518.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4998..5089 
                     /label="Myc-DDK"  
     misc_feature    5119..5704 
                     /label="PolyA signal"  
     misc_feature    5176..5195 
                     /label="Restriction Enzyme"  
     misc_feature    5980..6652 
                     /label="ColE1"  
     misc_feature    7082..7876 
                     /label="Kan/Neo"  
     misc_feature    7911..8213 
                     /label="SV40 ori"  
     misc_feature    8299..8776 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4980..4986 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4980 
                     /label="Col20a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCTCCAA GGCTCCTACC AACACTTCTG CCTCTGGATG TTCTTGGGCA 
     1081 CCACGTTGGC ACTGGGGCAG GGACAAGTAA GTAGCCGCCT GAGGCTGGCA GTACTGCCTG 
     1141 AGGACCAGCT GCAGATGAAG TGGAGGGAAG CAGAGGGAAG TGGCCTTGGC TTCTTGGTGC 
     1201 AGGTGACACC TATGGCAGGG GACTTGGAGC AGGAGCTGAT ACTGACCACC AAGACCCCCA 
     1261 AGGCCACTGT GGGTGGCCTG AACCCTTCCA AGAGCTACAC CTTGCAGATC TTTGAGCTCA 
     1321 CTGACTCGGG GCCTATCCTG CTGGCACGAA GGGAGTTTGT GATTGAGGAT CTGAAGAGCC 
     1381 AATCTTTGGG CAGAGGCAGC CGGAGGCTGG CTGGAGCCAC CCTGGAGCCT ACCTCCTTGC 
     1441 CTCTGAGGGG CCCAGACTCT GAGAAAACAT CTGAGCCCAG CATTGCCTTT ACCTTAAGCA 
     1501 GGGACCTGCC CATTCTTGAT CACCCCCAGT TCCAATGTAC ACCCCCAACA CCTGCTGATA 
     1561 TAATCTTCCT GGTGGACGGG TCCTGGAGCA TCGGCCACAA CCACTTCCAG CAGGTGAAAG 
     1621 ACTTCCTGGC CAGCATCATC ACACAATTTG CAATAGGACC AGATAAAGTC CAAGTAGGTC 
     1681 TGACACAGTA CAGTGGAGAC CCCCAGACCG AGTGGGACCT GAACTCCTTC CAAACCAAGG 
     1741 AGCAAGTGCT AGCAGCTGTA CACCACCTCC GCTACAAGGG AGGAAACACA TTCACAGGCC 
     1801 TTGCTCTAAC CCATGTGCTG GAGCAGAACC TGAAGCCAGC AGCCGGTGTC CGTCCAGAGG 
     1861 CAGCCAAAGT GTTGATTCTG GTGACAGATG GCAAGTCCCA GGATGATGTG CGCACAGCTG 
     1921 CCAGGATCCT TAAGGATCAG GACATTGATG TCTTTGTTGT GGGTGTGAAG AATGTCGATG 
     1981 AGGCTGAGCT GAAGCTCCTG GCGTCCCAGC CACTGGACAT CACTGTCCAC AACGTGCTGG 
     2041 ACTTCCCTCA GCTGGACACC CTAGCTCCTC TGCTCAGCCG ACTCATTTGC CAAAAAATTC 
     2101 AGGGCAGAGG CCCAGTCAAA CCAGCAGCAG GTACTCGGGT CCTGGACCCC CTCCCCACGC 
     2161 CCACCAGACT GATCCTGACC CACGCCACCT CATCCAGTAT CCACCTGTCT TGGACTCCAG 
     2221 CCTTATACCC ACCCCTCAAG TATCTAATTG TATGGCAGCC TTCTAGGGGT GGTGCCCCCA 
     2281 AGGAGGTGGT GGTGGAGGGG CCTGTCTCAT CCATGGAGCT TGGAAACCTG ACCTCCAGCA 
     2341 CAGAATACTT GGTCTCTGTG CTTCCTGTCT ATGAGAGTGG GGTTGGCAAG AGCCTGCAAG 
     2401 GCCGGGCAAC CACAGCACCC CTGCCTCCAC CTGGGCCACT GACCCTAGCT GCAGTGACAC 
     2461 CCAGAACTCT CCACGTCACC TGGCCGCCCT CTGCTGGGGT CACTCAGTAC TTGGTGCAGT 
     2521 ACTTGCTGGC TACCTCCACT GGGGAGGAGC AAAAGAGAGA GGTACATGTG GGACAGCCTG 
     2581 AGGTACTGCT GGATGGTCTG GAGCCTGGCC AGGACTATGA TGTCTCAGTG CAGAGCCTTC 
     2641 GGGGACCAGA GGCCAGCGAG GTCCAGAGCA TCCGTGCCAG GACCTCTGCC CTGGGGCCCC 
     2701 CAAGACACCT GACTTTCTCA GATGTTCGCT ACAACTCAAC CTGTGTGTCC TGGGAAGCCC 
     2761 AGAGGCCTGT GCGCCTGGTC AAGGTCAGCT ACATCTCTAG TGATGGCAGC CACTCTGGGC 
     2821 AGACTCAGGT TCCTGGGAAC CTTACTTCAG CTACTCTGGG CCCACTTTCT TCCTCTACCA 
     2881 TGTACACCGT CAGAGTCACC TGTTTTTACC TTGGGGGTGG TTCCTCCGTG CTGACTGGCC 
     2941 ATGTAACCAC ACAGAAAGCT CCTAGCCCTG GCCAGCTGTC TGTGATGGAG CTCCCGGGAG 
     3001 ATGCAGTAAA GCTGTCATGG CTGGCCACTG CCTTGTCTGG TGTACTTGTT TATCAGATCA 
     3061 AGTGGATGCC CTTGGGAGAA GGGAAGGCCC GTGAGATCTC TGTCCCAGGG ACCCTTGGCA 
     3121 CAGCCACCTT GCCTGGCCTG ATGAAACATG TGGAATATGA GATCACCATC CTTGCCTACT 
     3181 ACAGGGACGG CACTCGCAGC GACCCCGTGT CCCTCCGCTA CACCCCCTCT GCAGCAAGCA 
     3241 GAAGCCCCCC ATCCAGCCTG GCCTTGTCCT CGGAGACCCC CAACAGCCTG CAGGTCAACT 
     3301 GGACACCACC AAGTGGCCAT GTCCTCCATT ACCGGCTCAA CTACACACTG GCCTCAGGCT 
     3361 CTGGACCAGA GAAATCGATC TCTGTACCAG GCACCAGGAG CCATGCCGTG CTTCGTGATC 
     3421 TGATGTCTGC CACGAAGTAC AGGGTCCTGG TCTCAGCTGT CTACAGAGCA GGGGAGAGCA 
     3481 TGGCAGTGTC TGCCACATAC CGCACAGCAG CCTGCCCAGC CCTGCACCCG GACAGTTCCC 
     3541 TCTCAGGGTT TGACCTGATG GTGGCCTTTG GCCTCGTGGC AAAGGAGTAT GCCTCTATTC 
     3601 GAGGAGTTGC CATGGAGCCC TCAGCCTTGG GTGTGGTCCC AACCTTCACT CTCTTCAAGG 
     3661 ATGCTCAACT GATGCGTCGG GTCAGTGACA TCTACCCAGC AACCCTGCCA CCAGAACACA 
     3721 CCATTGTCTT CCTTGTGCGT CTACTCCCTG AGACACCCCG GGAAGCCTTT GCACTGTGGC 
     3781 AGATGATGGC TGAAGATTTC CAGCCCATCC TCGGGGTTCT GCTGGATGCT GGCAGAAAGT 
     3841 CTCTGACTTA CTTCAACCAT GATTCCCGGG CAGCCTTGCA GGAGGTCACC TTTGACCTGC 
     3901 AGGATGCAAA GAAGATCTTC TTTGGAAGCT TCCACAAGGT ACATATAGCT GTAGGCCACT 
     3961 CTAAGGTCAG ACTCTATGTG GACTGTCGGA AGGTGGCCGA GCGTCCCATT GGGGATGCTG 
     4021 GCAGCCCACC CACAGGTGGT TTCATCACAC TAGGGAGGCT AGCCAAGGCC AGAGGGCCAC 
     4081 GGAGCAGCTC AGCCACATTC CAGCTCCAGA TGTTGCAAAT CGTGTGCAGT GATACCTGGG 
     4141 CAGACAAGGA CAGGTGTTGT GAGATTCCTG CACTGAGGGA TGGAGAGACG TGCCCGGCCT 
     4201 TCCCTTCAGC CTGTGCCTAC TCATCTGAGA CCCCTGGGCC ACCAGGGCCC CAAGGCCCTC 
     4261 CGGGTCTCCC TGGAAGGAAC GGCCCTCCAG GACAGCAGGG GCACCCAGGG CCCAAGGGGG 
     4321 AGCCGGGCCC ACCTGGACAA ACAGGACCTG AAGGCCCTGG AGGTCAGCAG GGGTCACCAG 
     4381 GGACCCAGGG CCGCGCAGTC CAGGGACCCA TGGGGCCACC AGGAGCCAAA GGAGAGAAGG 
     4441 GGGATCAGGG ACTTTCAGGC TTGCAGGGCC TCTCTGGCCA GCAAGGCATC CCTGGGAAAA 
     4501 CTGGCCTACA GGGACCAAAG GGCATGAGAG GACTGGAGGG ACCTGCTGGC CTGCCAGGAC 
     4561 CACCTGGCCC CAGGGGGTTC CAGGGCTTGG CAGGGGCCAG GGGCACCAAT GGGGAGCGAG 
     4621 GAGCCCCAGG AGCTGTGGGT CCCACAGGTC TACCAGGGTC CAAAGGTGAA CGAGGAGAGA 
     4681 AGGGAGAGCC ACAGTCCCTT GCCACCATCT TCCAGCTGGT GAGCCAGGCC TGCGAGTCAG 
     4741 CCATACGGAC ACATGTGCTG AAGCTGAATT CCTTCCTCCA TGAGAATGCC CGTCCCCCAA 
     4801 TGCCCTTCAT GGCAGAGACA GCTAAGCTAG GCAGACCCAG ATCTATAGAT CCTGGATTAC 
     4861 ACAACGAGGC TCTACTTCCT GGAGACTGGG GACATATCCT CCGCCCTGAG GACCAAGGTG 
     4921 AGCCTGTGAC TATCAGCCAC ACAAGCAACC CTAGACTGCA GGAGGTGCAG ACCCCAGAAT 
     4981 CCCTGGAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5041 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5101 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5161 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5221 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5281 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5341 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5401 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5461 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5521 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5581 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5641 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5701 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5761 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5821 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5881 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5941 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6001 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6061 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6121 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6181 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6241 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6301 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6361 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6421 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6481 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6541 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6601 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6661 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6721 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6781 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6841 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6901 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6961 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7021 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7081 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7681 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7861 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7921 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7981 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8041 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8401 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8461 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8521 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8581 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8641 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8701 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8761 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8821 CAAAAATTTA ACGCGAATTT T
//