LOCUS       MR217481                8508 bp    DNA     circular  SYN 
DEFINITION  Ambra1 (Myc-DDK-tagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 2
ACCESSION   NM_001080754
VERSION     NM_001080754.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4665..4756 
                     /label="Myc-DDK"  
     misc_feature    4786..5371 
                     /label="PolyA signal"  
     misc_feature    4843..4862 
                     /label="Restriction Enzyme"  
     misc_feature    5647..6319 
                     /label="ColE1"  
     misc_feature    6749..7543 
                     /label="Kan/Neo"  
     misc_feature    7578..7880 
                     /label="SV40 ori"  
     misc_feature    7966..8443 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4647..4653 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4647 
                     /label="Ambra1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTTGTC CCAGAGAAGA ATGCTGTACG AATCCTCTGG GGCCGAGAAC 
     1081 GGGGCACTCG TGCCATGGGT GCCCAGCGGC TTCTGCAGGA GCTGGTGGAG GATAAGACTC 
     1141 GATGGATGAA ATGGGAGGGC AAGAGAGTAG AACTGCCTGA TAGTCCACGC TCGACCTTCT 
     1201 TACTGGCCTT CAGCCCAGAC AGGACTCTCT TGGCATCCAC ACATGTGAAC CATAACATCT 
     1261 ATATTACAGA GGTTAAGACT GGCAAGTGTG TTCACTCTCT GATCGGACAC CGCCGTACTC 
     1321 CATGGTGTGT CACTTTTCAC CCCACCATCT CAGGCCTTAT TGCTTCTGGT TGCCTAGATG 
     1381 GGGAAGTTAG GATTTGGGAT TTACATGGGG GCAGTGAAAG CTGGTTCACA GACAGCAACA 
     1441 ATGCCATAGC CTCCTTGGCT TTCCACCCTA CGGCTCAGCT TCTACTAATT GCCACTGCAA 
     1501 ATGAGATCCA TTTCTGGGAT TGGAGTCGTC GGGAGCCCTT TGCTGTGGTG AAAACAGCCA 
     1561 GTGAGATGGA GCGTGTCCGC CTGGTGAGAT TTGATCCACT TGGACACTAC TTACTTACAG 
     1621 CTATTGTTAA CCCTTCTAAT CAGCAGGGTG ATGACGAACC AGAAATCCCT ATAGATGGAA 
     1681 CAGAGTTATC TCATTACCGT CAACGTGCCC TCCTGCAATC ACAGCCAGTT CGCCGGACAC 
     1741 CTCTCCTCCA CAATTTCCTG CACATGCTGT CCTCCCGTTC CTCTGGCATC CAGACCGAGC 
     1801 CCTTCCATCC CCCGGAGCAG GCTTCATCAA CGCAGCAGGA CCAGGGCCTC CTGAACCGGC 
     1861 CGTCTGCCTT CAGTACAGTC CAGAGCAGCA CTGCGGGCAA CACGCTCCGC AACCTCAGTC 
     1921 TGGGTCCTAC CCGGCGCTCT TTGGGGGGCC CTTTGTCTAG CCACCCTTCT AGGTATCACA 
     1981 GAGAACTAGC CCCTGGGCTG ACAGGTTCTG AGTGGACCCG GACAGTACTC ACTCTGAACT 
     2041 CTCGTTCTGA GGTAGAATCT ATGCCCCCAC CGAGGACCAG TGCCTCCTCA GTGAGTTTGC 
     2101 TTTCTGTACT GAGACAGCAG GAAGGTGGCT CTCAGGCATC TGTGTATACT TCAGCCACAG 
     2161 AAGGGAGGGG TTTTCCATCA TCAGGGTTGG CAACTGAGTC AGATGGAGGG AATGGTTCCA 
     2221 GCCAAAACAA CTCAGGCAGC ATTCGCCATG AGCTTCAATG TGACCTGAGA CGCTTCTTTT 
     2281 TGGAGTATGA CAGGCTGCAG GAGCTGGATC AGAGCCTGAG TGGGGAAACT CCCCAGACCC 
     2341 AACAGGCCCA GGAAATGCTC AACAATAATA TTGAATCTGA GAGACCAGGT CCTTCCCACC 
     2401 TGCCTACCCC ACACAGCAGT GAGAACAACT CCAACCTGTC TCGGGGCCAC CTTAATCGAT 
     2461 GCCGTGCTTG CCATAATCTT CTAACCTTCA ACAATGATAC CCTTCGTTGG GAAAGAACCA 
     2521 CACCTAACTA CTCATCTGGT GAGGCTAGCT CTTCCTGGCA CGTCTCCACT ACCTTTGAGG 
     2581 GCATGCCACC AAGTGGCAAC CAGCTACCAC CTCTTGAGCG GACTGAGGGC CAAATGCCTA 
     2641 GCTCCAGCAG GCTGGAGTTG AGCAGCTCTG CTAGTTCACA AGAGGAGAGG ACTGTGGGGG 
     2701 TGGCCTTTAA CCAGGAGACA GGCCACTGGG AACGCATTTA TACCCAGTCC AGCAGATCTG 
     2761 GAACTGTATC ACAGGAGGCC TTACATCAAG ATATGCCTGA AGAAAGCTCT GAGGAAGACT 
     2821 CCCTCAGGAG GAGGCTGCTG GAGTCTTCCC TCATTTCATT ATCCCGTTAT GATGGAGCAG 
     2881 GATCCAGAGA GCACCCAATT TACCCAGACC CAGCGAGATT ATCTCCTGCT GCATACTACG 
     2941 CCCAGAGGAT GATCCAGTAT CTCTCAAGGA GAGACAGTAT TCGCCAGCGC TCCATGCGCT 
     3001 ACCAACAGAA TCGGCTTCGT TCTTCCACCT CCTCTTCTTC CTCAGACAAC CAGGGTCCAT 
     3061 CAGTAGAGGG AACCGACTTG GAATTTGAGG ACTTTGAGGA CAATGGTGAC AGATCTAGGC 
     3121 ACCGAGCTCC CCGAAATGCC CGAATGTCTG CACCTTCACT TGGACGATTT GTTCCAAGGC 
     3181 GTTTTTTGCT GCCTGAGTAC TTGCCTTATG CTGGGATTTT TCATGAACGT GGACAGCCTG 
     3241 GCTTGGCTAC TCATTCTTCT GTTAACAGGG TCCTGGCAGG GGCAGTAATT GGAGATGGAC 
     3301 AGTCTGCTGT GGCCAGTAAC ATTGCCAATA CTACCTACAG GCTCCAGTGG TGGGACTTCA 
     3361 CTAAGTTTGA CCTTCCAGAA ATCAGTAATG CCTCTGTGAA TGTCCTGGTA CAGAATTGCA 
     3421 AGATCTACAA TGATGCCAGC TGTGACATTT CTGCAGATGG TCAGCTCCTG GCAGCCTTCA 
     3481 TCCCGAGCAG CCAGAGGGGC TTTCCTGATG AAGGCATCCT GGCAGTGTAT TCCCTGGCCC 
     3541 CCCATAACTT GGGGGAAATG CTCTACACCA AGCGATTTGG TCCCAATGCC ATTTCTGTAA 
     3601 GCCTGTCCCC GATGGGCCGA TACGTTATGG TGGGCTTGGC CTCACGAAGG ATCCTACTGC 
     3661 ACCCCTCCAC AGAGCACATG GTAGCCCAGG TCTTCAGGCT GCAACAGGCC CATGGTGGTG 
     3721 AGACTTCCAT GAGGAGAGTT TTCAATGTCC TTTACCCCAT GCCTGCAGAC CAACGGAGAC 
     3781 ACGTCAGCAT CAACTCTGCC CGTTGGCTAC CCGAGCCAGG GCTTGGTTTG GCCTATGGCA 
     3841 CCAACAAAGG AGACTTGGTG ATCTGCCGAC CAGAGGCCTT AAACTCTGGC ATCGAGTACT 
     3901 ACTGGGACCA GCTAAGTGAA ACAGTCTTCA CTGTCCACTC AAGTAGCAGG AGCAGCGAGC 
     3961 GGCCTGGAAC CAGCAGAGCC ACGTGGCGGA CAGACAGAGA CATGGGTTTG ATGAATGCAA 
     4021 TTGGGCTGCA GCCTCGGAAC CCAACCACCT CTGTGACATC TCAGGGCACC CAGACTCTGG 
     4081 CCCTCCAGTT GCAGAACGCT GAAACACAGA CTGAGAGGGA GGAAGAGGAA CCAGGGGCAG 
     4141 CATCTTCAGG TCCTGGTGAA GGCGAGGGCT CAGAATATGG TGGCAGTGGG GAAGATGCCC 
     4201 TCAGCAGGAT CCAGAGGCTG ATGGCCGAGG GTGGCATGAC AGCTGTGGTA CAACGGGAAC 
     4261 AGAGCACCAC CATGGCCTCC ATGGGTGGCT TTGGCAACAA CATCATCGTT AGCCACCGCA 
     4321 TTCACCGCAG CTCCCAGACA GGCACAGAGT CAGGGGCTGC TCGTACCTCC TCACCCCAGC 
     4381 CCTCCACCTC TCGAGGACTG CCATCAGAAC CTGGGCAACT GGCAGAGCGA GCGCTAAGCC 
     4441 CCCGGACAGC CTCCTGGGAC CAGCCTAGTA CCTCTGGGAG GGAACTACCC CAGCCAGCCC 
     4501 TGTCCTCTTC CTCCCCTGTC CCCATTCCAG TCCCCCTTGC CAGCAATGAG GGACCAACCA 
     4561 TGCACTGCAA TGTGACCAAT AACAGTCACC TTCCTGAGGG TGATGGTAGC AACAGGGGGG 
     4621 AAGCTGCAGG CCCTAGTGGA GAGCCACAGA ACCGAACGCG TACGCGGCCG CTCGAGCAGA 
     4681 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4741 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4801 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4861 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4921 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4981 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5041 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5101 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5161 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5221 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5281 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5341 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5401 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5461 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5521 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5581 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5641 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5701 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5761 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5821 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5881 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5941 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6001 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6061 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6121 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6181 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6241 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6301 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6361 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6421 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6481 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6541 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6601 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6661 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6721 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6781 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6841 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6901 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6961 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7021 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7081 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7141 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7201 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7261 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7321 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7381 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7441 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7501 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7561 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7621 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7681 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7741 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7801 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7861 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7921 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7981 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8041 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8101 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8161 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8221 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8281 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8341 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8401 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8461 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//