LOCUS       MR217278                8790 bp    DNA     circular  SYN 
DEFINITION  Kdm2b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 3
ACCESSION   NM_001005866
VERSION     NM_001005866.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4947..5038 
                     /label="Myc-DDK"  
     misc_feature    5068..5653 
                     /label="PolyA signal"  
     misc_feature    5125..5144 
                     /label="Restriction Enzyme"  
     misc_feature    5929..6601 
                     /label="ColE1"  
     misc_feature    7031..7825 
                     /label="Kan/Neo"  
     misc_feature    7860..8162 
                     /label="SV40 ori"  
     misc_feature    8248..8725 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4929..4935 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4929 
                     /label="Kdm2b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCAGCTG CTGCGTGCGA TTGACCGCCA GAGATACGAC GAGAACGAAG 
     1081 ACTTGTCGGA CGTGGAGGAA ATTGTCAGCG TCCGTGGCTT CAGCCTGGAG GAGAAGCTAC 
     1141 GTAGCCAGTT ATACCAGGGG GACTTCGTGC ATGCTATGGA AGGCAAAGAT TTTAACTATG 
     1201 AGTACGTACA GAGAGAAGCT CTCAGGGTCC CCCTGGTTTT TCGGGACAAG GATGGACTAG 
     1261 GGATCAAGAT GCCAGACCCT GATTTCACAG TCCGAGACGT CAAACTCCTG GTGGGGAGCC 
     1321 GCCGTTTGGT GGATGTCATG GACGTCAACA CCCAGAAGGG TACCGAGATG AGCATGTCCC 
     1381 AGTTTGTGCG CTACTACGAG ACACCAGAGG CACAGCGGGA TAAACTGTAC AACGTCATCA 
     1441 GCCTCGAGTT CAGCCATACG AAGCTGGAGC ATCTGGTCAA GCGTCCCACT GTGGTGGACC 
     1501 TGGTCGACTG GGTGGACAAC ATGTGGCCTC AGCATCTAAA GGAAAAGCAG ACAGAAGCCA 
     1561 CCAATGCCCT TGCAGAGATG AAGTACCCCA AAGTGAAAAA GTACTGTCTG ATGAGCGTGA 
     1621 AGGGCTGTTT CACTGACTTC CACATTGACT TTGGAGGCAC CTCCGTGTGG TACCATGTGT 
     1681 TCCGTGGTGG CAAGATCTTT TGGCTGATCC CTCCAACCCT GCACAACTTG GCTTTGTACG 
     1741 AGGAGTGGGT GCTGTCTGGC AAACAGAGCG ACATCTTTCT GGGAGACCGC GTGGAACGCT 
     1801 GCCAAAGAAT TGAGCTGAAG CAAGGCTACA CCTTTTTCAT CCCTTCCGGT TGGATCCATG 
     1861 CGGTTTATAC GCCTGTGGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAT AGCTTCAACG 
     1921 TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGGAC CCGGGTTCAG CCCAAGTTCC 
     1981 GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATATGTG TACTGTGTGA 
     2041 CCCAGCGCTC CTACCTCACT CAGGAATACC AGAGAGAATT AATGCTTATT GATGCCCCAA 
     2101 GAAAAACCAG TGTAGACGGC TTTTCATCCG ACTCCTGGCT GGACATGGAG GAGGAGTCCT 
     2161 GCGAGCAGCA GCCACAGGAG GAAGAGGAGG AGGAGGAGGA CAAGGAGGAG GAAGGAGATG 
     2221 GTGCAGATAA AACACCCAAG CCACCCACCG ATGACCCCAC CTCACCCACC AGCACCCCGC 
     2281 CCGAAGACCA GGACAGCACA GGGAAGAAGC CTAAAGCCCC TGCCATACGG TTCCTCAAGA 
     2341 GGACGTTGTC CAATGAGTCA GAGGAAAGTG TCAAGTCGAC CTCGATGCCC ACGGACGATC 
     2401 CCAAGACGCC CACGGGCTCC CCGGCCACCG AGGTTTCTAC CAAGTGGACT CACCTTACCG 
     2461 AATTTGAACT GAAGGGCTTG AAAGCCCTGG TTGAAAAGCT AGAGTCCCTT CCGGAGAATA 
     2521 AGAAGTGTGT CCCTGAGGGA ATCGAGGACC CCCAGGCCCT CCTGGAAGGT GTAAAGAATG 
     2581 TACTGAAAGA GCACGTGGAT GACGACCCCA CCCTGGCCAT CACCGGGGTC CCTGTGGTCA 
     2641 GCTGGCCAAA GAAAACTGCA AAGAACCGGG TGGTGGGTCG GCCTAAGGGC AAGTTGGGCC 
     2701 CGGCCTCAGC GGTGAAGTTG GCTGCCAACC GAACAACAGC AGGAGCTCGC AGGCGCCGGA 
     2761 CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG TGGAGAGTGC CACTTTTGCA 
     2821 AGGACATGAA GAAGTTTGGA GGTCCTGGGC GCATGAAGCA GAGCTGCATC ATGCGGCAGT 
     2881 GCATCGCGCC AGTGCTGCCC CACACCGCCG TGTGCCTTGT GTGTGGCGAG GCAGGGAAGG 
     2941 AGGACACAGT GGAAGAGGAA GAAGGCAAGT TTAACCTCAT GCTCATGGAA TGCTCCATCT 
     3001 GCAACGAGAT CATCCACCCT GGATGCCTTA AGATTAAGGA ATCGGAGGGT GTGGTCAACG 
     3061 ATGAGCTTCC CAACTGCTGG GAGTGTCCGA AGTGTAACCA TGCCGGCAAG ACCGGGAAAC 
     3121 AAAAGCGTGG CCCTGGCTTT AAGTATGCCT CCAACCTGCC TGGCTCCTTG CTCAAGGAGC 
     3181 AGAAGATGAA CCGGGACAAC AAGGAAGGGC AAGAGCCTGC CAAGCGGAGA AGTGAGTGTG 
     3241 AAGAGGCTCC CCGTCGGAGG TCAGACGAGC ACCCCAAAAA GGTGCCTGCA GATGGCATCC 
     3301 TCCGCCGAAA GTCTGATGAT GTGCACCTGA GGAGGAAGCG GAAATACGAG AAGCCCCAAG 
     3361 AGCTGAGTGG ACGCAAGCGA GCCTCGTCGC TTCAAACGTC CCCCGGTTCC TCCTCTCACC 
     3421 TCTCGCCGAG GCCCCCTCTA GGCAGCAGTC TCAGCCCTTG GTGGAGATCC AGTCTCACTT 
     3481 ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA AAGCGGCGGT 
     3541 CCTGGAAGAA CGCTGAGGAT CGTCTGTCAC TGGCCAACAA GCCCCTTCGG CGCTTTAAGC 
     3601 AGGAACCGGA GGACGATCTG CCTGAGGCAC CTCCTAAGAC CCGGGAGAGT GATCAGTCAC 
     3661 GTTCCAGCTC ACCCACTGCT GGTCCCAGCA CTGAGGGAGC TGAAGGCCCA GAAGAGAAGA 
     3721 AAAAGGTGAA GATGCGCCGG AAGCGGCGAC TTGTTAACAA GGAGCTGAGC AAAGAGCTAA 
     3781 GCAAGGAGCT CAACCATGAG ATCCAAAAGA CGGAGAGCAC CCTGGCTCAC GAGAGCCAGC 
     3841 AGCCCATCAA GTCAGAGCCT GAGAGCGAGA ACGACGAGCC CAAGAGGCCC TTAAGCCACT 
     3901 GCGAGCGCCC CCACCGCTTC AGCAAAGGGC TCAACGGCAC ACCTCGGGAG CTGCGGCACT 
     3961 CGCTGGGACC TGGCCTGCGT AGTCCACCTC GTGTTATGTC CCGGCCCCCG CCCTCTGCAT 
     4021 CCCCACCCAA GTGCATCCAG ATGGAGCGTC ACGTGATCCG GCCACCGCCC ATCAGCCCCC 
     4081 CACCTGACTC GCTGCCCCTG GATGATGGAG CAGCCCACGT CATGCATAGG GAGGTGTGGA 
     4141 TGGCAGTCTT CAGCTACCTC AGCCACCGAG ACCTGTGTGT CTGCATGCGG GTCTGCAGGA 
     4201 CCTGGAACCG CTGGTGCTGC GATAAGCGGT TGTGGACCCG CATCGACCTG AACCGCTGCA 
     4261 AGTCCATCAC ACCCCTGATG CTGAGCGGTA TCATCCGGCG ACAGCCTGTC TCCCTTGACC 
     4321 TCAGTTGGAC CAACATCTCC AAGAAGCAGC TGAGTTGGCT CATCAACCGG TTGCCTGGGC 
     4381 TCCGAGACTT GGTGCTGTCA GGCTGCTCAT GGATCGCTGT CTCAGCCCTC TGTAGCTCCA 
     4441 GTTGTCCATT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGACTAAAG GATGCCCAGA 
     4501 TGAGGGATCT CCTGTCTCCA CCCACAGACA ACAGGCCAGG TCAGATGGAC AATCGGAGCA 
     4561 AGCTCCGGAA CATTGTAGAG CTGCGCCTAG CTGGCCTGGA CATCACAGAT GTCTCCCTGC 
     4621 GGCTCATTAT TCGCCATATG CCCCTGCTCT CGAAGCTCCA ACTCAGTTAC TGTAACCACA 
     4681 TCAATGACCA GTCCATCAAC CTGCTCACTG CCGTCGGCAC CACCACCCGA GACTCGCTGA 
     4741 CAGAGGTCAA CCTATCAGAC TGTAATAAGG TAACTGACCT GTGCCTGTCC TTCTTCAAAC 
     4801 GCTGTGGAAA TATCTGTCAT ATTGACCTGA GGTACTGCAA GCAAGTCACC AAGGAAGGCT 
     4861 GTGAGCAATT CATAGCTGAA ATGTCTGTGA GTGTCCAATT TGGGCAAGTG GAAGAGAAAC 
     4921 TCCTGCAAAA ACTAAGTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4981 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5041 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5101 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5161 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5221 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5281 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5341 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5401 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5461 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5521 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5581 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5641 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5701 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5761 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5821 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5881 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5941 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6001 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6061 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6121 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6181 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6241 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6301 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6361 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6421 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6481 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6541 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6601 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6661 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6721 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6781 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6841 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6901 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6961 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7021 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7081 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7141 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7201 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7261 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7321 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7381 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7441 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7501 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7561 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7621 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7681 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7741 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7801 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7861 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7921 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7981 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8041 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8101 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8221 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8281 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8341 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8401 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8461 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8521 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8581 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8641 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8701 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8761 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//