LOCUS       MR217258                7722 bp    DNA     circular  SYN 
DEFINITION  Megf11 (Myc-DDK-tagged) - Mouse multiple EGF-like-domains 11 (Megf11), transcript variant 2
ACCESSION   NM_172522
VERSION     NM_172522.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3879..3970 
                     /label="Myc-DDK"  
     misc_feature    4000..4585 
                     /label="PolyA signal"  
     misc_feature    4057..4076 
                     /label="Restriction Enzyme"  
     misc_feature    4861..5533 
                     /label="ColE1"  
     misc_feature    5963..6757 
                     /label="Kan/Neo"  
     misc_feature    6792..7094 
                     /label="SV40 ori"  
     misc_feature    7180..7657 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3861..3867 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3861 
                     /label="Megf11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCGTCT GCGGTGGGCC TCCTTGTCTT CCTTCTGCAA GCTGCTCTTG 
     1081 CTCTGAACCC TGAAGACCCC AATGTGTGTA GCCACTGGGA GAGCTATGCC GTGACTGTGC 
     1141 AGGAGTCTTA TGCACACCCC TTTGATCAGA TCTACTACAC ACGATGTGCA GACATCCTCA 
     1201 ACTGGTTCAA GTGTACCCGG CACCGGATCA GCTATAAGAC CGCGTATAGG CGCGGCCTCC 
     1261 GGACCATGTA CCGGCGGAGG TCCCAATGCT GCCCTGGCTA CTATGAGAAC GGAGACTTCT 
     1321 GCATTCGCTG TGACAGCGAG CACTGGGGTC CCCACTGCAG CAACCGGTGT CAGTGTCAGA 
     1381 ACGGCGCCCT GTGCAACCCT ATCACCGGCG CCTGCGTGTG CGCCCCGGGC TTCCGAGGCT 
     1441 GGCGCTGTGA GGAACTCTGC GCTCCTGGTA CTCACGGCAA GGGCTGCCAG CTGCTCTGTC 
     1501 AGTGCCACCA TGGCGCCAGC TGTGACCCGC GCACTGGCGA GTGCCTCTGC GCTCCTGGCT 
     1561 ACACAGGCGT TTACTGTGAG GAGCTGTGCC CCCCTGGGAG CCATGGAGCT CACTGTGAGC 
     1621 TGCGCTGCCC CTGCCAGAAT GGAGGCACCT GCCACCACAT CACTGGCGAA TGTGCCTGCC 
     1681 CTCCAGGCTG GACGGGAGCA GTGTGTGCCC AGCCCTGCCC TCCAGGGACC TTTGGCCAGA 
     1741 ACTGTAGCCA GGACTGTCCC TGCCACCATG GAGGCCAGTG TGACCATGTG ACTGGACAAT 
     1801 GCCACTGTAC AGCTGGATAC ATGGGGGACA GGTGTCAAGA AGAATGTCCC TTTGGAACGT 
     1861 TCGGTTTCCT GTGCTCTCAA CGCTGTGACT GCCACAATGG AGGTCAATGT TCACCAGCCA 
     1921 CAGGGGCCTG TGAGTGTGAG CCTGGCTACA AGGGCCCTAG CTGCCAGGAG CGGCTATGCC 
     1981 CTGAGGGCCT GCATGGCCCA GGCTGCACCT TGCCCTGCCC CTGTGACACC GAGAACACTA 
     2041 TCAGCTGCCA TCCAGTTACT GGAGCTTGTA CCTGCCAACC AGGCTGGTCT GGCCACTACT 
     2101 GCAATGAGTC CTGCCCCGCC GGCTACTATG GCAACGGTTG CCAGCTACCC TGCACCTGCC 
     2161 AGAACGGTGC TGACTGCCAC AGTATCACCG GGAGCTGCAC TTGTGCTCCA GGCTTCATGG 
     2221 GAGAGGTGTG TGCCGTCCCC TGTGCTGCAG GGACCTATGG TCCCAACTGT TCATCTGTAT 
     2281 GTAGCTGTAG CAACGGCGGC ACCTGTTCCC CAGTGGATGG CTCCTGCACC TGCCGAGAGG 
     2341 GATGGCAGGG CCTGGACTGC TCCCTGCCTT GTCCCAGTGG GACCTGGGGC CTGAACTGCA 
     2401 ATGAGACTTG CATCTGTGCC AATGGAGCTG CCTGCAGCCC CTTTGATGGG TCCTGTGCCT 
     2461 GCACCCCAGG CTGGCTGGGG GACTCCTGTG AACTGCCCTG CCCGGACGGC ACTTTTGGGC 
     2521 TGAACTGCAG TGAGCATTGC GACTGCAGCC ATGCTGATGG CTGTGACCCT GTCACAGGCC 
     2581 ACTGCTGCTG CCTGGCAGGA TGGACAGGCA TCCGCTGTGA TAGCACGTGT CCTCCAGGTC 
     2641 GCTGGGGCCC CAACTGTTCA GTGTCCTGCA GCTGTGAGAA CGGAGGTTCC TGCTCCCCGG 
     2701 AGGACGGGAG CTGCGAGTGT GCCCCTGGCT TTCGAGGACC CTTATGTCAG AGAATCTGCC 
     2761 CACCAGGATT CTACGGCCAT GGCTGCGCCC AGCCTTGTCC CCTCTGCGTG CACAGCAGGG 
     2821 GGCCCTGCCA CCACATCAGT GGTATCTGTG AGTGCCTGCC AGGATTCTCT GGAGCCTTGT 
     2881 GCAACCAAGT GTGTGCTGGA GGGCACTTCG GGCAGGACTG TGCCCAGCTC TGTTCCTGTG 
     2941 CCAACAATGG GACCTGCAGC CCCATCGATG GCTCCTGTCA GTGCTTCCCT GGGTGGATTG 
     3001 GCAAGGACTG CTCACAGGGT TGCCCTTCAG CATTTTTTGG GAAGGACTGT GGGCACATAT 
     3061 GCCAGTGTCA GAATGGAGCC AGCTGTGACC ACATCACTGG GAAATGCACC TGTCGAACAG 
     3121 GCTTCTCTGG CCGCCACTGT GAACAGAGAT GTGCCCCTGG AACCTTTGGA TATGGGTGTC 
     3181 AGCAGCTATG TGAGTGCATG AACAATGCCA CTTGTGACCA CGTCACTGGT ACCTGTTACT 
     3241 GTAGCCCGGG ATTCAAAGGA ATCAGGTGTG ACCAAGCTGC CCTCATGATG GATGAGCTGA 
     3301 ATCCCTACAC CAAGATCAGT CCAGCTCTGG GAGCAGAGCG GCACTCAGTG GGTGCTGTCA 
     3361 CCGGCATCGT TCTCCTGTTG TTCCTGGTGG TGGTGCTGCT GGGCCTGTTT GCCTGGCGAC 
     3421 GGAGGCGGCA GAAAGAGAAA GGCCGTGACC TGGCTCCCCG AGTCTCCTAC ACCCCAGCCA 
     3481 TGAGGATGAC CAGCACAGAC TACTCTCTCT CAGATTTGTC TCAAAGTAGC AGCCATGCCC 
     3541 AGTGCTTTTC CAATGCCAGC TACCACACAC TGGCGTGTGG GGGGCCTGCC ACCAGCCAGG 
     3601 CCAGCACTCT GGACAGGAAC AGCCCCACCA AGCTCAGTAA CAAGTCCCTT GACAGAGACA 
     3661 CAGCAGGCTG GACCCCCTAC AGCTATGTGA ACGTGTTAGA CTCCCATTTC CAGATCAGTG 
     3721 CCCTGGAGGC CAGGTACCCG CCCGAGGACT TCTACATTGA ACTTAGACAC CTCAGCCGCC 
     3781 ATGCTGAGCC ACACTCACCA GGCACTTGTG GAATGGACAG ACGTCAGAAC ACATACATTA 
     3841 TGGACAAAGG CTTCAAAGTT GCACCTGCTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3901 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3961 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4021 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4081 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4141 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4201 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4261 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4321 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4381 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4441 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4501 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4561 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4621 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4681 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4741 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4801 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4861 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4921 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4981 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5041 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5101 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5161 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5221 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5281 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5341 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5401 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5461 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5521 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5581 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5641 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5701 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5761 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5821 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5881 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5941 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6001 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6061 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6121 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6181 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6241 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6301 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6361 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6421 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6481 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6541 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6601 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6661 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6721 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6781 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6841 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6901 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6961 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7021 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7081 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7141 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7201 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7261 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7321 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7381 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7441 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7501 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7561 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7621 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7681 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//