LOCUS       MR217252                8181 bp    DNA     circular  SYN 
DEFINITION  Aasdh (Myc-DDK-tagged) - Mouse aminoadipate-semialdehyde dehydrogenase (Aasdh)
ACCESSION   NM_173765
VERSION     NM_173765.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4338..4429 
                     /label="Myc-DDK"  
     misc_feature    4459..5044 
                     /label="PolyA signal"  
     misc_feature    4516..4535 
                     /label="Restriction Enzyme"  
     misc_feature    5320..5992 
                     /label="ColE1"  
     misc_feature    6422..7216 
                     /label="Kan/Neo"  
     misc_feature    7251..7553 
                     /label="SV40 ori"  
     misc_feature    7639..8116 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4320..4326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4320 
                     /label="Aasdh"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCTTCAG GAGTTGGTGC TTCGGACTGC CTCTGTCTAT ATGGATAGAA 
     1081 CAGCTGTATG TTTTGACGAA GGCAATAACC AGCCTCCAGT GTGCTATTCC TACAAGGCTC 
     1141 TGTTGAGCGC GGCTTCAGAA TTATCCCATT TTCTGATAGC ACACTGTGAC TTTGGAGGAA 
     1201 TTCGGGAAAT TGGTCTCTAT TGTCAACCTG GGATAAACTT ACCCTCCTGG ATTTTAGGGA 
     1261 TTCTTCAGGT CCCCGCAGCG TATGCGCCCA TTGATCCAGA CTCCCCACCG TCCTTATCAA 
     1321 CTTACTTTAT GAAAAAATGT GACCTAAAGT ATGTTCTCGT TGAAAAACAG CAGCTTAGTA 
     1381 AATTCAAATC TTCACATGAG ACAGTTCTGA ACTATGACAC AGTTTCCGTG GAACACAAGG 
     1441 ACCTGGCACT CTTCAGACTG CATTGGGAAG ATGGTCGGGT GAGCACAGTG CTTGGTGACA 
     1501 GAGCAGATCA GCACAAGGTG ACGGATAGAG AAGACAGAGT GAGTGCTGAG AGCAGGACCC 
     1561 CAGAGAAGGA GCACATGGAC ATGCGGCATG ACGGTTGCTT GGCTTATGTC CTCCATACCT 
     1621 CAGGGACCAC GGGGACACCG AAGATTGTCA GAGTGCCCCA TGCATGCATA CTGCCTAATA 
     1681 TCCAGCACTT CCGGTCACTT TTTGACATCA CTCAAGAAGA CATTTTGTTT CTGGCTTCTC 
     1741 CTCTGACCTT CGATCCATCT GTTGTGGAGA TATTTGTTTC TCTGTCCAGT GGGGCCTGCC 
     1801 TGCTTATCGT GCCAACTTCT GTCAAAGTGC TACCATCAAA ATTAGCTGAC ATTCTCTTTT 
     1861 CCCGCCACAG AGTGACTGTT TTACAGGCAA CGCCAACATT GCTGAGAAGA TTTGGATCTG 
     1921 AGCTCATCAA GTCCACTGTC CTGTCAGCGC ATACGTCTCT GAGAGTCCTG GCCCTCGGTG 
     1981 GGGAAGCCTT CCCCTCGCTG ACCATCCTCA AAAGCTGGCG AGGCAAAGGC AACAGAACCC 
     2041 AGATATTTAA CATCTATGGT ATCACAGAGG TATCCAGCTG GGCCACTTTT TACAGGATCC 
     2101 CAGAGGAGAT TCTTAATTCT GCCGTGAAAC ATGAATCCCC TGTGCAGCTG GGGTCGCCAC 
     2161 TACTTGGAAC GGTGATTGAA GTCAGAGACC AAAATGGTTC TCCAGTTCTC GAAGGCACTG 
     2221 GCCAAGTATT TTTAGGTGGG AAGAACAGAG TGTGTTTTCT TGATGATGAA ATGACAGTGC 
     2281 CCCTTGGCAC CATGAGAGCC ACAGGAGACT TTGTGACTGT GAAAGATGGA GAGATATTTT 
     2341 TCTTGGGAAG AAAGGACAGC CAGATCAAGC GCCATGGCAA ACGTCTTAAC ATTGCACTTG 
     2401 TGCAACAGGT TGCTGAAGAA CTTCGCCAGG TGGAGTCCTG CGCGGTCACT TGGTATAATC 
     2461 AGGAAAGATT GATTCTCTTC ATCGTATCCA AAGTTGACTT AGTAAAGGAC TGCATCTTTA 
     2521 AAGAATTGCA GAAACACCTT CCAGCACACG CCCTGCCTGA TGACATGGTG CTGATCGATA 
     2581 CCCTGCCATT TACATGCCAT GGCAAAGTTG ATGTTTCTGA GTTAAACAAG ATATATTTAG 
     2641 ACTACATAAG CTCACAGCCT AGGAATGAAC TCCATGGAAA AGAGGAACTT TGGGGAAAAT 
     2701 TACAGTATTT ATGGAAGTCT ATTCTGTGTC TCCCTGAGGA TCCAGAGGAT ACTCTGAAGG 
     2761 TTCCCGCCAA CTCCGTCTTT TTAGATAGTG GTGGGGATTC CCTCAAGTCC ATGCGGCTCC 
     2821 TCAGTGAGAT TGAGAGGCTC ACCGGGACAG CCATCCCTGG CCTTCTGGAA GTTATCCTCA 
     2881 GCAGTTCCCT CTTAGACGTT TACAACCACA TCGTTCAAGC CGTGTTCACA CCGGAGGACA 
     2941 GGAAAGCCAA CAGGAGTTAC ACCACAAAAA GGAAATTCAG TGATGCTGAT CCAGAGGAAG 
     3001 CCAGCGGGAA GCCTGCACGT CTGGAGTCTG CCTGGCCTTC GAACCATGCC GGCGAGACCA 
     3061 ACTCCGTGAT CGCTCTGAGC AGAGGGAGTC AGGTTTTGTC TCTTGGCGCT GGGAGGCTTT 
     3121 TAACTCAGTT AGGACTCTGC CTGCCAGTCT GTTCTCTGGA TTTAATCCCA CAGACTAACA 
     3181 CTCAGATATT AAAAAGCCTA AGTCCTCCAG CTCCTGATGA AAACTTGGAA AAGCCCCCAC 
     3241 TTTTTCAGCA GGGGAGCCCC GTGGTTGGGG CTATGGCGAT GGCATTGCGG GAGAGGTGGC 
     3301 GGTCAGACAC AGGCAAATGC GTTGATGCCT CCCCTCTGCT GGTGAGAGCG GCTGTACAGG 
     3361 ATAAGCCCTC CACAACCGTG TATATTGGCT CGCACTCGCA CACAGTGAAG GCTGTCGACC 
     3421 TGTCCTCTGG GGAGACGAGG TGGGAACAGC TTCTGGGAGA TCGAATTGAA TCCTCAGCGT 
     3481 GTGTGTCTAA ATGTGGAAAC TTTATTGTAG TGGGCTGTTA TAATGGATTA GTCTATGTCC 
     3541 TAAAAAGTAA TAGTGGAGAA AAATATTGGA CATTTACTAC TGAAGATGCT GTCAAAAGCT 
     3601 CACCAGCCGT GGATCCAACC ACAGGACTCA TTTATGTCGG ATCTCATGAC CAGCACGCAT 
     3661 ATGCTTTAGA CATTTATGAA AAGAAATGTG TCTGGAAGCT AAACTGTGAA GGCGCTCTCT 
     3721 TTTCCTCTCC GTGTGTGAGC CTGAGTCCAC ACCATCTGTA CTGTGCTACG CTAGGGGGAC 
     3781 TCTTACTGGC CCTAAATCCT GCTTCTGGGA GCACGGTGTG GAAGCGCTCC TGTGGAAAGC 
     3841 CACTCTTCTC TTCCCCACGG TGCTACCAGC AATACATCTG CATCGGCTGC GTGGACGGCA 
     3901 GTTTGCTCTG TTTCACACAC TCAGGGGAGC AGGTCTGGCG CTTTGCTGCT GGAGGGCCAA 
     3961 TCTTCTCATC CCCATGCGTC TCGGCAGCAG AACAAGAAAT ATTTTTTGGT TCCCATGACT 
     4021 GCTTTATCTA CTGCTGTAGC AAGGAAGGTC ACCTCCGGTG GAAATTTGAG ACGACAGCCA 
     4081 GGGTGTATGC AACGCCCTTT GCTTTCAGTA ACCACCCCCG TAGCGATGAT GCACTGCTGG 
     4141 CAGCGGCATC CACTGACGGG AAACTGTGGG TCCTCGAGTC TCGGAGCGGA GAGCTGCGAA 
     4201 GTGTGTATGA ACTCCCCGGA GAAGTCTTCT CCTCTCCTGT GGTCTGGGAA TCAATGCTTG 
     4261 TTATTGGGTG CAGAAACAAT TATATTTACT GTCTGGATTT ATTGTGTGGT GATAAAAATA 
     4321 ACCAGGTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4381 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4441 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4501 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4561 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4621 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4681 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4741 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4801 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4861 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4921 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4981 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5041 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5101 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5161 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5221 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5281 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5341 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5401 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5461 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5521 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5581 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5641 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5701 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5761 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5821 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5881 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5941 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6001 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6061 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6121 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6181 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6241 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6301 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6361 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6421 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6481 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6541 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6601 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6661 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6721 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6781 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6841 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6901 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6961 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7021 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7081 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7141 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7201 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7261 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7321 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7381 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7621 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7681 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7741 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7801 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7861 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7921 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7981 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8041 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8101 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8161 CAAAAATTTA ACGCGAATTT T
//