LOCUS       MR217244                9315 bp    DNA     circular  SYN 
DEFINITION  Plb1 (Myc-DDK-tagged) - Mouse phospholipase B1 (Plb1), transcript variant 1
ACCESSION   NM_001081407
VERSION     NM_001081407.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5472..5563 
                     /label="Myc-DDK"  
     misc_feature    5593..6178 
                     /label="PolyA signal"  
     misc_feature    5650..5669 
                     /label="Restriction Enzyme"  
     misc_feature    6454..7126 
                     /label="ColE1"  
     misc_feature    7556..8350 
                     /label="Kan/Neo"  
     misc_feature    8385..8687 
                     /label="SV40 ori"  
     misc_feature    8773..9250 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5454..5460 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5454 
                     /label="Plb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGTAC CCAGGTGTGT CCCCGGTGGG ACTGCTGCTG CTGCTACTGC 
     1081 TGGGACAAGG GCCCTCCCAA ATCCATGGCT CTTCTGGAGA GAACACATTG GCATGGCAGT 
     1141 CCCAGCAAGT GTTTTGGACC CTGAAGAATT TTCCATTCCC TTGCAAGCCA AAGAAGTTAG 
     1201 AATTGAGTGT GCTTTCTGAG TCAGTTCACT CTCTGAGACC CTCAGACATT AAACTCGTGG 
     1261 CAGCCATCGG CAACCCAGAA ATTCCTCTAG CCCCTGGGTC TGGCACGATC AACATGGAGA 
     1321 AACCTCAAAG CATCAAGAAC CAACCACAGG ATGTATGCAT GGGAATCATG ACAGTTCTTT 
     1381 CAGATATCAT CAGACATTTC AGTCCTTCTG TCCTGATGCC CACGTGTTCT CCTGGGAAGG 
     1441 GCACTGCAGT CCACACTACT GCTGAAGATT TGTGGATTCA GGCTAAAGAG CTGGTGAGGC 
     1501 GCCTGAAGGA CAACCCGCAA CTTGACTTTG AGAAGGACTG GAAACTCATC ACTGTGTTCT 
     1561 TCAGTAACAC AAGCCAGTGT CACCTGTGTC CCTCTGCTCA GCAGAAAAGT CACTTGATGA 
     1621 GGCACATGGA GATGCTGTGG GGGGTGCTGG ATTACCTGCA TCATGAGGTC CCCAGAGCAT 
     1681 TTGTGAATTT GGTGGATCTC TCTGAGGTTT TGGCCATGGA TCTTCAGCAT CAAGAGACTG 
     1741 GCTTCAGCCC TGCACCAGAG GTTTGCAAAT GCACAGAGAC AACGACGCTA TCCAAAGCTG 
     1801 TCATGCAGTG GTCCTATCAG GAAGCCTGGG AAGATCTTCT GGCCTCTAGC AAGTTCAACA 
     1861 AGCATGAGAC CTTCGCGGTG GTTTTCCAGC CTTTCTTCGA TGAAATTGAA CCACCCTTGA 
     1921 AAAGGTCCTC GCCCCAGGAT CCCACCACAC TCGCCCTCAG GATCTGGAAT AGCATGATGG 
     1981 AACCAGTGGG TCAAAAGGAT GGGCTACTCA ATACTGCAGA GAGAAAGACA ATGAAATGTC 
     2041 CCTCTGAGGA GAGTCCCTAT CTGTTCACCT ACAAAAATAG CAACTACCAG GCCAGACGAC 
     2101 TGAAACCTAT AACAAAGCTT CAGATGAAAG AAGGATCAGA ATTCACCTGT CCTGACAAGA 
     2161 ACCCCTCGAA CTCAATCCCC ACAACAGTTC ACAGCTTGCG GCCAGCTGAT ATCAAGATCA 
     2221 TAGGAGCATT GGGTGACTCG CTCACGGCAG GCAATGGAGC TGGGGCCAGC CCTTGGAATA 
     2281 TCTTGGATGT CTTAACTGAA TACCGAGGCC TGTCCTGGAG TGTGGGCGGG GATGAGACCA 
     2341 TCAAAACCGT GACCACCCTA CCCAACATTC TCCGGGAATT CAACCCCTCC TTGAAGGGCT 
     2401 TTTCTGTTGG CACTGGGAAA GAAAGCACTT CCCGAGCATC CTTCAACCAG GCCGTAGCAG 
     2461 GAGCCAAATC TGATGGCTTA GCTGGCCAGG CCAGAAAGCT GGTGGACCTG ATGAAGGCAG 
     2521 ACAAGACAAT AAACTTTCAG GAAGACTGGA AGATAATCAC TGTGTTTATA GGAGGCAATG 
     2581 ACCTCTGTGC TTCCTGCAGT AACTCGACTC GCTTTTCTCC CCAAAACTTC ATAGACAACA 
     2641 TCAAGAACGC CCTGGACATC CTCCATGCAG AGGTTCCACG GGCCTTTGTG AACATGGCAA 
     2701 TGGTGATGGA GATCACCCCC TTGAGAGAAC TATTCAATGA ACCTACAGTC TCATGCCCAC 
     2761 GGAACATCCT CAGCCGACTG TGTCCTTGTG TCCTGGGCCT TGGTGATAAC TCAGAAGAAC 
     2821 TTTCCAGCCT CGTTCAAAGG AACAGGGATT ATCAGAAGAA AACTGAAGAG CTCATTAATA 
     2881 GCGGACGATA TGACACGAGG GATAATTTCA CTGTGGTTGT CCAGCCCTTA TTTGAAAATG 
     2941 TGTCCATGCC ACGGACCCCG GAGGGCGTGC CCGACAAGTC TTTCTTTGCC CCTGACTGTT 
     3001 TCCACTTTAA TGCCAAGACT CATGCCCGCT CAGCCATTGC CCTCTGGAAG AACATGCTGG 
     3061 AACCTGTGGG CCACAAGACA AGACATAATA ATTTTGAAAT CAAGGCCCCT ATCGTGTGTC 
     3121 CGAACCAGGC CTCGCCATTT CTGAGCACTA CCAAGAACAG CAACCTGGGT AATGGAACCT 
     3181 GGATGGTGTG CGAGGAGAGA GCCCCTTCTG CCTCACCACC AACCTCAGTG CATACCCTGA 
     3241 GACCTGCAGA CATTCAAGTT GTGGCAGCTC TGGGAGACTC TCTGACTGCT GGCAATGGAA 
     3301 TCAGCTCCCA AGAAGGTAAC CTCACCGATG TCTCCACACA GTATCGAGGA CTGTCCTATA 
     3361 GTGCTGGTGG GGACAAGACC CTGGAGAATG TGACCACTTT GCCCAATATC CTACGGAAAT 
     3421 TTAATGGAAA TCTCACAGGC TACTCAGTAG GAACCGGTGA CTCCAGTTCC GCAAACGCTT 
     3481 TCCTTAACCA GGCTGTCCCT GGGGCGAAGG CTGAGAACCT TACAAGCCAA GTCCGGACTC 
     3541 TGGTTCAGAA GATGAAGAGT GACAACAGAG TGAACTTCAA CCGAGACTGG AAGGTCATCA 
     3601 CGGTGATGAT CGGCGCCAGT GACTTGTGTG ACTTCTGCAC GGATTCGAAT CATTACTCTG 
     3661 CAGCCAATTT TTTTGACCAT CTCCAAAATG CCTTGGACAT TCTACATAAG GAGGTACCCA 
     3721 GAGCCCTGGT CAACCTTGTG GACTTCATTA ACCCCAGTAT CATTCGGGAA GTATTCCTGA 
     3781 AGAACCCAGA CAAGTGCCCA GTGAATCAGT CCAGTGTCCT GTGCAACTGT GTTCTGACCC 
     3841 CGAGGAAGGA TTCCTATGAG CTGGCAAGGT TGGAGGCCTT CACCAAATCC TACCAGAGTA 
     3901 GCATGCTCCA ACTGGTTGAG TCAGGCCGCT ACGACACTCG AGAGGACTTC TCTGTGGTAC 
     3961 TGCAGCCCTT TCTCCTCAAC ACCAAGCTCC CTGTCCTAGA GAACGGGAAA CCAGATACAT 
     4021 CCTTCTTTGC CCCAGACTGC ATCCACCTAA ACCAGAAGTT CCACACTCAG CTCGCGAGAG 
     4081 CCCTCTGGGC CAATATGCTT GAACCCCTGG GAAAGAAAAC TGATACTTTG GACCCAAAAG 
     4141 GACACATATC TTTGGCCTGC CCCACCAAGG ATCAGCCCTT CCTGAGAACC TTCCGAAACA 
     4201 GTAACTACAA GTATCCTACC AAGCCAGCCA TTGAGAACTG GGGCAGTGAC TTCCTGTGCA 
     4261 CAGAGAAGAG CCCTTCCAGC CAGGTACCCA CCTCAGTTCA TGAGCTCCGA CCAGCAGACA 
     4321 TCAAGGTGGT GGCAGCAATG GGTGACTTTC TGACTACAGC CACCGGAGCT CGGCCAAGTG 
     4381 GATATAAAAG GCTAGCCACG CCCTGGAGGG GGCTGTCTTG GAGCATTGGA GGAGATGGAA 
     4441 AGTTGGAGAC CCATACCACA CTTCCCAACA TCCTGAAGAA GTTCAACCCT TCCATCACTG 
     4501 GATTCTCTAC TGGTACCCTG GACAACAAGG CAGGATTAAA TGTGGCAGAA GAAGGAGCCA 
     4561 GAGCTCAGGA CATGCCGGCC CAGGCTAAGA CCCTGGTGAA GAAGATGAAA AGCACACCTA 
     4621 CAATCAACCT ACAGGAAGAC TGGAAGCTGA TTACACTCCT CATTGGAAAC AACGACCTGT 
     4681 GTCTTTACTG TGAGAATCCG GAGGACAACT CAACTAAAGA GTATGTCAAG TACATCCAGC 
     4741 AAGCCTTGGA CATCCTCTAT GAGGAGCTTC CCAGAGTTTT CATCAATGTG GTGGAAGTCA 
     4801 TGGAGCTGGC TGGCCTGCAC CACGTCCAAG GTGGGAAATG TGCCATGCCA CTGGCAGTTC 
     4861 AGAAAAACTG CAGTTGCTTA AGACACTCCC AAAACCTCAC AGCGATGCAG GAGCTGAAGA 
     4921 AACTAAACTG GAACCTCCAG AGCGGCATCT CCGAGCTCTC CTATTGGCAC CGGTACATGG 
     4981 AGCGTGAGGA CTTCGCAGTC ACTGTGCAGC CTTTCTTCCG GAATACCTTT ATCCCACTGA 
     5041 ATGAGCGTGA GGGCCTGGAC CTCACTTTCT TCTCTGAAGA CTGTTTCTAC TTCTCAGACC 
     5101 GTGGGCATGC TGAGATGGCC ATTGCCCTCT GGAATAACAT GCTGGAACCA GTGGGCTGGA 
     5161 AGACATCCTC CAATAACTTC ATATACAACA GAACCAAACT CAAGTGCCCC TCACCTGAAA 
     5221 GGCCTTTCCT CTACACCCTC CGGAATAGTC AGCTTCTTCC AGACAAGGCT GAAGAACCCT 
     5281 CCAATGCACT CTACTGGGCA GTGCCAGTGG CAGCAATAGG TGGCCTGGCA GTTGGCATCC 
     5341 TTGGAGTGAT GTTGTGGAGA ACTGTGAAAC CCGTCCAACA GGAGGAGGAG GAGGAGGACA 
     5401 CTCTTCCAAA TACAAGTGTG ACCCAGGATG CTGTATCAGA AAAGAGGCTC AAAGCTGGGA 
     5461 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5521 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5581 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5641 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5701 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5761 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5821 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5881 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5941 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     6001 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     6061 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6121 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6181 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6241 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6301 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6361 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6421 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6481 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6541 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6601 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6661 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6721 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6781 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6841 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6901 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6961 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     7021 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     7081 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7141 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7201 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7261 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7321 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7381 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7441 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7501 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7561 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7621 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7681 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7741 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7801 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7861 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7921 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7981 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     8041 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     8101 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8161 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8221 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8281 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8341 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8401 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8461 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8521 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8581 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8641 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8701 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8761 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8821 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8881 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8941 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     9001 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     9061 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9121 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9181 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9241 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9301 TTTAACGCGA ATTTT
//