LOCUS       MR217166                8427 bp    DNA     circular  SYN 
DEFINITION  Arhgap20 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 20 (Arhgap20)
ACCESSION   NM_175535
VERSION     NM_175535.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4584..4675 
                     /label="Myc-DDK"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    4762..4781 
                     /label="Restriction Enzyme"  
     misc_feature    5566..6238 
                     /label="ColE1"  
     misc_feature    6668..7462 
                     /label="Kan/Neo"  
     misc_feature    7497..7799 
                     /label="SV40 ori"  
     misc_feature    7885..8362 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4572 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4566 
                     /label="Arhgap20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGCGATG TCTCCGCAGC AGGATGCTCT AGGGGCGCAG CCGGGGCGCT 
     1081 CCTCCTCGCT GACAGGCATG TCTCGCATCG CGGGAGGCCC TGGCACCAAG AAGAAAATGA 
     1141 AAACACTCGC AGAAAGGAGG AGAAGCGCCC CGTCCCTCAT CCTGGACAAA GCCTTACAGA 
     1201 AGCGGCCGAG CACCAGAGAC AGCCATTCTG CCAGCATTGA CACGTGTGCA TTCCTCTCGT 
     1261 CCTTCATGTG CTCCAGTCGC ACTCTGCTGA TTGATGGTCC AGTGGAACTT AAAAGAGGCC 
     1321 TGCAGAGGCA GGAGAGGCAT CTTTTCCTAT TCAACGATCT GTTTGTCTCA GCCAAAATCA 
     1381 AATATAACAA CAACTTTAAG ATAAAGAATA AAATAAGACT AACTGACATG TGGACAGCTA 
     1441 GCTGTGTGGA GGAAGTGGGA GAAGGCAACA TGAATGCTCA GAAGTCCTTT GTCCTGGGCT 
     1501 GGCCCACCGT GAACTTTGTG GCTACTTTCA GTTCTCCAGA ACAAAAGGAC AAATGGCTGT 
     1561 CTCTCCTTCA GAGATACATC GCTCTAGAAA AGGAGAAAGA CTACCCGAAG AGCATCCCCC 
     1621 TCAAAATCTT TGCCAAGGAC ATTGGAAACT GTGCCTATTT TAAAACTATA ACAGTGATGA 
     1681 ATTCAGACAC AGCGAGTGAA GTTATCAACA TGTCACTGCA AATGTTAGGA ATAACTGGCT 
     1741 CTGAGAGAGA TTACCAACTG TGGGTAAATT CTGGAAAAGA AGCGGCACCG TACCCACTCA 
     1801 TTGGACACGA GTATCCTTAT GGAATTAAAA TGAGCCACCT CCGAGACACT GCCCTTCTGA 
     1861 CGCAGGGATC AAGAGACTCT GCCAGCCCAT CCCAGCTCCA AGAGCCTTTC CTCATGGAGC 
     1921 AGCTGCCCCG CGAGATGCAA TGCCAGTTCA TCCTGAAACC TACCCGCCTG GCGACAGCCC 
     1981 AGCAACTGAG CGATTCCAGC CAGAAGACAT TTAAAAGGAG GCGCTCTATC ATCAACTGGG 
     2041 CTTTTTGGCG GGGTTCTAGC ACACACCTGG ACAACCTGCC CATGTCACCA ACATCCCCTA 
     2101 TGCCAGGACA GCTCTTTGGA GTTTCTTTAC CAGATCTTTG TGAGAACGAC AATCTGCCAA 
     2161 AGCCTATCTT GGATATGCTT TCCTTCCTTA ACCAAAAAGG CCCCCTCACA AAAGGCATCT 
     2221 TCAGGCAGTC GGCCAACATG AAATCTTGCA GAGAGCTAAA GGAGAAACTG AATTCTGGAA 
     2281 TCGAAGTCCA CCTTGACTGT GAATCCATCT TTGTGATAGC ATCTGTGTTG AAGGATTTTC 
     2341 TGAGAAATAT CCCAGAAAGT ATCTTTTCAT CAGATCTATA TGATCACTGG GTCTGTGTAA 
     2401 TGGATCAAGG AAATGATGAA GAAAAAATAA ATATAATTCA AAGGCTATTA GATCAGCTTC 
     2461 CCAGAGCCAA TGTTGTTTTC CTAAGGTATC TGTTTGGGGT ATTACACAAC ATTGAACAAC 
     2521 ATTCCTTGTC TAATCAGATG ACTGCATTTA ACTTAGCAGT GTGTATCGCT CCAAGCATTC 
     2581 TTTGGCCTCC TGCTTCCTCC AGTCCAGAGC TAGAAAATGA ATTTACAAAA AAGGTTTCTC 
     2641 TTCTTATACA ATTTCTGATT GAAAACTGCT GTAGGGTATT TGGAGAAGAA ATCGCTTCTC 
     2701 TCTTGGGGGA GCTTTCGGAG AGAAGCGACA GAGAACACAC CCCAGATATA ACTTGCTTCC 
     2761 AAATGAACGA CTCCTCCTAT GACAGCTTGG AAAACGAGCT CAATGAGGAA GCTGATGCTC 
     2821 CATGCAGTGA CCTGGTCAAA AAGCTTGGCC AGGGTAGCAG GAGCATGGAC TCTGTCTTAA 
     2881 CCCTCAGCGA CTATGACCTT GAACAGCCTG AGGTGGAAGG CCTCTTAACC CTGAGCAACT 
     2941 TTGACTTAGA TCAGTCTAAA GAGGAGCACA TTCCGATAAA GCCACCTCTG GAGCCCAAGC 
     3001 CAGTGAATGT TTTCGTAGGC TACAGGAAGG TTTCACTAGG GGAGCACGCT AGGGCCCCAG 
     3061 CTGGCCCAGG CACACTCAGC TGCCTGCCTG TGGCTGCAGC AGATGCCCCG AAAGTCCTGC 
     3121 GGCGGCACCG GCGCTCCTCA GAACCCAGCA TTGACTATCT AGACACAAAG CTTTCCTATC 
     3181 TCCGGGAGTT TTACCAGAAA AAGCTGCGCA AGTCCAGCTG CGATGCTGTC CTCTCTAGAA 
     3241 AAGATGAAGA TTACCTGAAG CAGACCCAGC CTCAGAAGAA AGGGGATAAG GTGTGTCTTA 
     3301 AACAGAGTTC AGTGACAGGC ACTGACGTCA GCAAGAGAAA CACAGCTAAT GAAAACATTA 
     3361 AGAAGAAAAG CTTGTCTGGT CATGAAGGAA CTCAAGTGAC ACTTTTTACT AAGTCCAAGC 
     3421 CAGTACCCAT CTCTGTGGCA TCTTACAGCC ACGGGTCCTC CCAGGATCAT CCCAGGAAGC 
     3481 AAGCCTTTGA TGCAGACCCA TGCCGGTTCT CCCCACCACA CCTAACAGAT GCCCAAAAGA 
     3541 GCTCAAGGGT GCAACATCGG CGCTGTTCAG AACCCAGCAT AGATGACCAG AACTACAAGC 
     3601 TGTCCTATCT CAGGGGGATC TATTCAATGA AACAAAATAA AGCCAGCTGT GAGGCTGGCC 
     3661 TCTTGCATGG GGAGGACGAC TATCTCAGAA GGCATAAGTC TTTGCAAATC GAGGGCCAAA 
     3721 AGCTTATTAA TCAGAGTTTG GTCATGGGCA TTGAGGTGGG CAAGAGCAGC AGCTCTCACC 
     3781 AAAGCACTGA GAAGGTTTTG CCCCCAAGAC TAAACCTTTG CCCAAGGGCA AGCTATTCAA 
     3841 GCTTATCTTC TCCAGGCTCG TCCCCATCCG GCTCCTCTGT AAGCTCCCAG GACAGCGCTT 
     3901 TCTCTCAGAT CTCTGAACAC TCCGTGTTTA CGCCCACCGA AACCTCCTCA CCAATAGATT 
     3961 GCACTTTCCA GACACAAAGA AAACAAGAAG AGCTGTCTTC AGACTTTGAC AGTCCCAGCC 
     4021 GCCTTTCCGG AATGCCCGGT CCCTCCATGG GTCAGGCCAG CAGCCACCTA GCTTATTTAA 
     4081 GAAAGGGTAC CACGGAGCAG CCATCACAAA TGCATTCTGT AACCCTTCAC CCCAGTGCGT 
     4141 GGTTGAGAAG TGGCTTGGTC ACTTTAAAAA ACTGGTCCCT CAAAAAGAAA ACAAAAGCAG 
     4201 CCAGACCAGA AGACAGGAAA GTCTGTTCCC TGAAAGAGCC TCTGGAGTTG CCGTCATGTG 
     4261 CTTCCGGTAC CCCAGAGGCT GACTCTCTAC AAGAGAGTCA GGATGACTTA CAGGGAGATG 
     4321 AAGGACCTGG GCAAACAGCC TGTGGATTCA GTTCTTATGC CTGTCAGGAC TCAGAGCAAC 
     4381 ATGCCGGCTC TCCATTCCAC CTGGCAGAAA GCAGACTCAA GCCTTGTATG AAGTTATATA 
     4441 AGGGAGAAGA GAGTGGAGGG CAGTACCCTT GTGATAACCC TTGGGAAGGA GCCTCTTCAA 
     4501 GTCTGGAGAC CACGGAGGAT ACAGCCAACC CAGGTGCAGA ACCTACAACA TTTGCGATGA 
     4561 CGGGGACAGA TATTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//