LOCUS       MR217119                8178 bp    DNA     circular  SYN 
DEFINITION  Srgap3 (Myc-DDK-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 3 (Srgap3)
ACCESSION   NM_080448
VERSION     NM_080448.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4335..4426 
                     /label="Myc-DDK"  
     misc_feature    4456..5041 
                     /label="PolyA signal"  
     misc_feature    4513..4532 
                     /label="Restriction Enzyme"  
     misc_feature    5317..5989 
                     /label="ColE1"  
     misc_feature    6419..7213 
                     /label="Kan/Neo"  
     misc_feature    7248..7550 
                     /label="SV40 ori"  
     misc_feature    7636..8113 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4317..4323 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4317 
                     /label="Srgap3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCATCTCAA ACTAAGTTCA AGAAAGACAA AGAGATCATT GCTGAATATG 
     1081 AAGCCCAAAT AAAAGAGATC CGCACGCAGC TGGTGGAGCA GTTCAAATGC CTGGAGCAGC 
     1141 AGTCCGAGTC GCGGCTGCAG CTGCTGCAAG ACTTGCAGGA GTTCTTCCGC AGGAAAGCTG 
     1201 AGATTGAGCT GGAGTATTCC CGAAGCCTGG AGAAGCTGGC TGAGCGCTTC TCCTCCAAGA 
     1261 TTCGCAGCTC CCGGGAGCAC CAGTTCAAGA AGGATCAATA CCTCCTCTCG CCCGTGAACT 
     1321 GTTGGTATCT GGTACTGCAT CAGACTCGGC GGGAGAGCCG AGACCATGCC ACCCTCAACG 
     1381 ACATCTTCAT GAACAATGTC ATCGTCCGCC TCTCCCAGAT CAGTGAAGAT GTCATCAGAC 
     1441 TCTTCAAAAA GAGCAAAGAG ATTGGCCTGC AGATGCATGA GGAGCTGCTG AAGGTCACCA 
     1501 ACGAGCTCTA CACAGTCATG AAAACCTACC ACATGTACCA TGCAGAGAGC ATCAGTGCAG 
     1561 AAAGCAAGCT GAAGGAGGCT GAGAAGCAGG AGGAGAAACA GTTTAACAAG TCAGGAGAGC 
     1621 TAAGCATGAA CCTGCTCCGG CACGAGGACA GACCCCAGCG CCGCAGCTCC GTGAAGAAGA 
     1681 TTGAGAAGAT GAAGGAGAAG AGGCAGGCCA AGTATTCTGA GAACAAGCTC AAGTGCACGA 
     1741 AGGCCCGGAA TGATTACCTG CTGAATCTGG CAGCCACCAA CGCAGCTATC AGCAAATACT 
     1801 ACATCCACGA TGTCTCTGAT CTCATCGATT GCTGTGATTT GGGCTTTCAC GCCAGCCTGG 
     1861 CCCGCACTTT TCGGACCTAC CTCTCAGCAG AATATAACCT GGAGACCTCT CGCCATGAGG 
     1921 GTCTGGACGT CATAGAGAAT GCAGTGGACA ACCTGGACTC CCGGAGTGAC AAGCACACAG 
     1981 TCATGGATAT GTGCAGCCAG GTCTTTTGCC CTCCACTCAA GTTTGAGTTC CAGCCCCACA 
     2041 TGGGGGATGA GGTCTGCCAG GTCAGTGCAC AGCAGCCTGT CCAGACAGAG CTGCTCATGC 
     2101 GGTACCACCA GCTGCAGTCC AGGCTGGCCA CTCTCAAGAT TGAGAATGAA GAGGTTCGGA 
     2161 AAACCCTGGA TGCCACCATG CAGACCTTAC AGGACATGCT GACCGTGGAA GACTTCGATG 
     2221 TCTCTGACGC CTTCCAACAC AGCCGATCTA CAGAGTCCAT AAAGTCAGCT GCTTCTGAGA 
     2281 CCTACATGAG CAAAATCAAC ATCGCCAAAA GGAGAGCCAA CCAGCAGGAA ACAGAAATGT 
     2341 TTTATTTTAC AAAATTTAAA GAATATGTGA ATGGCAGTAA CCTCATCACC AAGCTGCAAG 
     2401 CCAAGCATGA CTTACTCAAG CAGACCCTGG GTGAAGGGGA GAGAGCAGAG TGCGGCACGA 
     2461 CCAGGCCCCC CTGTCTTCCC CCTAAACCAC AGAAAATGAG GAGACCTAGG CCTCTTTCAG 
     2521 TGTATAGCCA TAAACTCTTT AACGGCAGTA TGGAAGCGTT CATTAAGGAT TCAGGACAAG 
     2581 CTATACCACT TGTAGTTGAG AGCTGTATTC GATTCATCAA TTTATATGGA CTCCAGCAGC 
     2641 AGGGGATCTT CAGAGTTCCA GGGTCTCAGG TTGAAGTCAA TGACATCAAG AATTCCTTTG 
     2701 AGAGAGGTGA AGACCCCCTC GTGGATGACC AAAATGAGCG AGATATCAAT TCAGTTGCTG 
     2761 GTGTTTTAAA ACTGTATTTC CGAGGACTGG AAAACCCACT CTTTCCTAAG GAAAGGTTTC 
     2821 AAGATTTGAT ATCTACTATT AAACTGGAGA ACCCAGCTGA CAGGGTGCAC CCAATCCAGC 
     2881 AGATCCTCAT CACCCTGCCC CGCGTGGTCA TTGTGGTCAT GAGATACCTC TTTGCTTTCC 
     2941 TCAATCACCT TTCCCAGTAC AGTGATGAGA ACATGATGGA TCCCTACAAC TTGGCCATCT 
     3001 GCTTCGGGCC CACCCTCATG CACATCCCCG ATGGGCAGGA CCCTGTATCC TGCCAGGCGC 
     3061 ATGTCAATGA AGTCATCAAA ACTATCATCA TCCACCATGA AGCCATTTTC CCCAGCCCTC 
     3121 GGGAGCTAGA GGGACCCGTG TACGAAAAAT GCATGGCTGG AGGGGAAGAG TACTGTGACA 
     3181 GCCCACACAG TGAGCCGGGG ACCATTGATG AAGTTGACCA TGACAATGGC ACGGAGCCTC 
     3241 ACACCAGTGA CGAAGAAGTG GAGCAGATTG AGGCCATCGC CAAGTTTGAC TATGTGGGGC 
     3301 GGTCCCCTCG TGAACTATCC TTCAAAAAAG GGGCCTCACT ACTCCTGTAT CACCGTGCCT 
     3361 CAGAGGACTG GTGGGAGGGC CGCCACAACG GTGTAGATGG ACTCATCCCT CATCAGTACA 
     3421 TAGTTGTACA GGACATGGAC GATGCCTTCT CCGATAGCCT GAGCCAAAAG GCTGACAGCG 
     3481 AGGCCAGCAG TGGGCCACTG CTGGATGACA AGGCCTCTTC CAAGAATGAC CTCCAGTCCC 
     3541 CCACAGAGCA CATCTCTGAT TACGGCTTTG GGGGGGTGAT GGGCCGAGTG CGACTACGGT 
     3601 CCGACGGAGC AGCCATCCCC AGGCGCCGAA GTGGGGGCGA CACACACAGC CCACCCCGGG 
     3661 GCTTGGGTCC CAGCATAGAC ACGCCACCCC GAGCTGCTGC CTGCCCCAGC AGCCCCCACA 
     3721 AAATCCCCCT CAGCCGGGGA CGGATCGAGA GCCCTGAGAA GAGAAGGATG GCGACTTTCG 
     3781 GGAGCGCTGG CAGTATCAAC TACCCTGACA AGAAGGCGCT GACAGAAGGG CTCTCCATGA 
     3841 GGTCGACTTG CGGTTCCACG CGACACAGCA GCCTAGGGGA CCACAAGTCC CTGGAGGCTG 
     3901 AGGCCCTGGC AGAAGACATC GAGAAGACCA TGAGCACCGC TCTGCATGAG TTGCGGGAAC 
     3961 TTGAGAGACA GAACACTGTC AAGCAGGCAC CAGATGTGGT GCTGGACACC CTGGAGCCCC 
     4021 TGAAGAATCC ACCAGGCCCC ATCAGCTCAG AGCCCGCCAG CCCCCTGCAC ACCATTGTCA 
     4081 TCCGAGACCC TGATGCTGCT ATGCGCCGAA GCAGCAGCTC ATCGACGGAG ATGATGACCA 
     4141 CCTTCAAGCC AGCCCTGTCA GCTCGCCTGG CTGGTGCCCA GCTGCGTCCG CCCCCCATGC 
     4201 GTCCGGTCAG ACCTGTGGTT CAGCACCGAT CCAGCAGCAG CAGCAGCTCA GGTGTGGGCA 
     4261 GCCCGGCAGT GACACCCACG GAGAAGATGT TCCCTAATAG CTCCTCGGAC AAGTCTGGCA 
     4321 CCATGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4381 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4441 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4501 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4561 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4621 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4681 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4741 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4801 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4861 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4921 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4981 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5041 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5101 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5161 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5221 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5281 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5341 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5401 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5461 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5521 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5581 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5641 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5701 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5761 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5821 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5881 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5941 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6001 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6061 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6121 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6181 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6241 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6301 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6361 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6421 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6481 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6541 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6601 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6661 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6721 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6781 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6841 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6901 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6961 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7021 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7081 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7141 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7201 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7261 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7321 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7381 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7441 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7501 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7621 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7681 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7741 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7801 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7861 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7921 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7981 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8041 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8101 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8161 AAATTTAACG CGAATTTT
//