LOCUS       MR216999                7068 bp    DNA     circular  SYN 
DEFINITION  Arhgap28 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 28 (Arhgap28)
ACCESSION   NM_172964
VERSION     NM_172964.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3225..3316 
                     /label="Myc-DDK"  
     misc_feature    3346..3931 
                     /label="PolyA signal"  
     misc_feature    3403..3422 
                     /label="Restriction Enzyme"  
     misc_feature    4207..4879 
                     /label="ColE1"  
     misc_feature    5309..6103 
                     /label="Kan/Neo"  
     misc_feature    6138..6440 
                     /label="SV40 ori"  
     misc_feature    6526..7003 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3207..3213 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3207 
                     /label="Arhgap28"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGTGGAG GACTCGGGCG GCGTGGTGCT GACCGCCTAC CACTCGCACG 
     1081 CGCGCTCGCA ACCGCAGGGC GCTGAGCCAC GCTGCGCGTC CCGGGCCAGC CATCCGCTCA 
     1141 GCAGAAAATC CATTCCTCGC TGCCGACGAA TCAACAGGAT GCTCTCCAAT GAATCCCTGC 
     1201 ATCCTCCCTC CTTTAGTCGC TCCAACTCTC AAGCCTCAGT AGACAGCAGC GCATCCATGG 
     1261 AGGAGTTCCT GCGGGAAATT GAGAGTATCA AAGAGAGCAG CGTGGGGGCG TCGCAGGAGC 
     1321 AGCCACCCAC TGCTGCTGCT GCTGCTGCTG AAGTCAAGCC AGTGGATGAA GGAGAGCTTG 
     1381 AAGCGGAGTG GCTACAAGAT GTGGGATTGT CAACTCTGAT CTCTGGCAAC GAAGAGGAAG 
     1441 ACGGTAAAGC GCTCCTGTCT ACATTGACTC GGACCCAAGC TGCTGCAGTG AAAAAGAGAT 
     1501 ACAACACATA TACTCAGACT CTGAGGAAAA AGAATAAACA ACCCGTCAGG GATGTCAGAG 
     1561 ACATCTTCGG AGTCAGCGAA TCTCCTCCTA GTGATTCTTG TGAGCATGCT ACTCAGTTGG 
     1621 ACGGTACCAA GGAAGAAAAA GATCTGCCAG GAGTTACCAA GACAAGCAGA CCTCTGCCAG 
     1681 ACGATGCTTC TCTCAGTAGT ACCACCCTAT CCAATGGCGC CCAGGATGAA GAAGGTGGTT 
     1741 TTGTGGCCCT ACAGAGTGGT TCTGTGTCAA TACTTGAGGC TATCCCGGAT ATTGCTGTTC 
     1801 ACACCAATGG ATCTGCAGAT GCTGAGCAGT CGGTTCAGAG CACATTGAGT GATGATGATT 
     1861 ATCACGGGAA GAACGTTCCA GCAGAGGCTG AAGAGCTGTC CTTTGAGGTC TCTTACTCAG 
     1921 AAATGGTGAC AGAAATGCCT GATAGAAATA AATGGAAGAA GTCGGACATT AAGAAAGAAG 
     1981 ACTATGTCTT AACTAAATTT ATCATTCAGA AAACAAGATT TGGGTTGACC GAGACGGGGG 
     2041 ACCTGTCTGT GGAAGACATG AAGAAAATCC GCCACCTCTC TCTGATTGAG CTGACAGCCT 
     2101 TCTTCGATGC TTTTGGGATA CAACTGAAGA GAAACAAAAC CGAGCGAGTG AGAGGCCGAG 
     2161 ACAACGGGAT TTTCGGAGTG CCACTTACAG TCCTCCTGGA CAATGACCGG AAGAAGGACC 
     2221 CTGCAGTGAA AGTTCCCCTG GTGTTACAAA AATTTTTTCA GAAAGTCGAG GAATCAGGTC 
     2281 TGGAATCGGA AGGAATTTTT CGACTCTCGG GATGTACTGC CAAAGTCAAG CAGTACCGCG 
     2341 AAGAACTGGA TGCCAGGTTC AATGCTGACA AGTTCAAGTG GGACAAGATG TGTCACAGAG 
     2401 AAGCTGCGGT CATGTTGAAA GCATTCTTCA GAGAGCTGCC CACCTCTCTC TTCCCTGTTG 
     2461 AATATATACC CGCCTTCATC TCTCTGATGG AAAGAGGACC TGACATCAAA GTGCAGTTTC 
     2521 AAGCCTTGCA CCTCATGGTC ATGGCCCTGC CTGATGCCAA CAGGGACACA GCGCAGGCTC 
     2581 TGATGGCATT CTTCAATAAA GTGATCGCCA ACGAATCCAA GAACCGCATG AACCTGTGGA 
     2641 ACATTTCCAC AGTGATGGCG CCCAACTTGT TCTTCAGCAG AAGCAAGCAC TCTGACTGCG 
     2701 AGGAACTGCT GCTGGCCAAC ACGGCGACCC ACATCATCCG CCTGATGCTG AAGTACCAGA 
     2761 AGATTCTGTG GAAGGTTCCA TCTTTCTTGA TCACCCAGGT CAGAAGGATG AATGAGGCCA 
     2821 CCATGCTGTT GAAGAAGCAG CTCCCGAGTA TGAAGAAGCT GCTCAGGAGG AAGACCCTGG 
     2881 ATCGAGAGGT TTCAATCCTC AAGACCTCAA AGGTACCACA AAAATCACCT TCCTCAAGAA 
     2941 GAATGTCTGA TGTGCCAGAA GGTGTCATAA GGGTCCACGC TCCACTTCTG TCCAAAGTGT 
     3001 CCATGGCCAT TCAGCTCAAC AGTCAGACCA AAGCCAAAGA TATCCTGGCG AAATTTCAGT 
     3061 ATGAGAACAG TCATGGTTCA TCTGAACATA TTAAGATGCA GAACCAAAGG TTATATGAAG 
     3121 TTGGAGGAAA TATAGGACAA CACTGTTTAG ATCCTGATGC ATATATATTG GATGTGTATC 
     3181 ATATAAATCC TCATGCAGAA TGGGTCATCA AGCCCACGCG TACGCGGCCG CTCGAGCAGA 
     3241 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3301 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3361 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3421 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3481 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3541 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3601 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3661 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3721 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3781 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3841 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3901 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     3961 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4021 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4081 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4141 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4201 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4261 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4321 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4381 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4441 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4501 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4561 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4621 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4681 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4741 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4801 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4861 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4921 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     4981 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5041 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5101 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5161 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5221 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5281 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5341 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5401 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5461 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5521 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5581 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5641 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5701 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5761 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5821 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5881 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     5941 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6001 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6061 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6121 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6181 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6241 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6301 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6361 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6421 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6481 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6541 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6601 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6661 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6721 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6781 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6841 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6901 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     6961 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7021 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//