LOCUS       MR216888                9018 bp    DNA     circular  SYN 
DEFINITION  Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 1
ACCESSION   NM_001166416
VERSION     NM_001166416.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5173..5264 
                     /label="Myc-DDK"  
     misc_feature    5294..5879 
                     /label="PolyA signal"  
     misc_feature    5351..5370 
                     /label="Restriction Enzyme"  
     misc_feature    6155..6827 
                     /label="ColE1"  
     misc_feature    7257..8051 
                     /label="Kan/Neo"  
     misc_feature    8086..8388 
                     /label="SV40 ori"  
     misc_feature    8474..8951 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5148..5155 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5148 
                     /label="Med23"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTACCGATG GAGACGCAAC TGCAGAGCAT TTTCGAGGAG GTGGTGAAAA 
     1081 CAGAAATTAT AGAAGAGGCC TTCCCAGGAA TGTTTATGGA TACCCCCGAG GATGAAAAAA 
     1141 CAAAACTAAT TAGCTGCTTG GCGGCCTTCC GGCAGTTTTG GAGTGGACTT TCTCAGGAGT 
     1201 CTCATGAACA GTGTGTTCAG TGGATTGTTA AATTTATTCA TGGCCAACAC AGCCCCAAAA 
     1261 GAATTTCTTT CCTCTATGAC TGCTTAGCAA TGGCAGTTGA GACTGGTCTC CTTCCACCCA 
     1321 GGATGGTTTG TGAATCTTTG ATAAACTCTG ACTCTCTTGA ATGGGAAAGA ACACAGCTTT 
     1381 GGGCCTTAAC ATTTAAGCTG GTTCGGAAAA TCATTGGAGG AGTGGATTAC AAGGGTGTTC 
     1441 GAGACCTTCT CAAAGCGATT TTGGAGAAAA TCTTGACAAT CCCAAATACA GTGAGCTCTG 
     1501 CTGTTGTCCA GCAGCTTCTG GCAGCAAGAG AGGTTATAGC TTATATCTTA GAACGAAATG 
     1561 CCTGCCTATT GCCGGCCTAC TTTGCAGTCA CAGAGATCAG AAAACTATAT CCTGAGGGAA 
     1621 AACTTCCACA CTGGTTACTA GGAAACTTGG TATCAGACTT TGTGGATACC TTCAGGCCCA 
     1681 CAGCAAGGAT TAACTCCATT TGTGGCCGAT GCAGTCTTCT GCCAGTTGTG AACAACTCGG 
     1741 GAGCCATCTG TAACTCATGG AAACTGGACC CTGCAACTCT GCGATTTCCT TTGAAAGGCC 
     1801 TTTTGCCATA TGATAAGGAT CTGTTTGAAC CGCAGACTGC TTTGTTGAGA TATGTACTGG 
     1861 AGCAACCTTA TTCCAGGGAT ATGGTCTGCA ATATGCTAGG TTTAAATAAG CAGACCTTGA 
     1921 ACATTGCTCA GCACAAGCAG CGCTGCCCTG TGCTGGAGGA CCAGTTGGTG GACCTGGTGG 
     1981 TGTACGCCAT GGAGAGGTCA GAGACCGAGG AGAAGTTTGA CGATGGGGGA ACCAGCCAGC 
     2041 TCCTGTGGCA GCACCTTTCC AGCCAGCTCA TTTTCTTTGT GCTTTTCCAG TTTGCCAGTT 
     2101 TCCCACATAT GGTCCTCTCT CTCCACCAGA AGTTAGCAGG GCGAGGACTG ATTAAAGGGC 
     2161 GAGACCATCT GATGTGGGTG CTGCTGCAGT TCATTTCTGG AAGTATTCAG AAAAATGCCC 
     2221 TCGCTGACTT CCTCCCTGTC ATGAAGCTCT TTGACCTGCT GTACCCGGAG AAGGAGTGTA 
     2281 TCCCAGTTCC TGATATTAAC AAACCCCAGT CAACGCACGC CTTTGCAATG ACTTGTATTT 
     2341 GGATTCATCT TAATAGAAAA GCTCAAAATG GCGACTCCAC GCTCCAGATC CCAATACCCC 
     2401 ATTCCCTGAA GCTGCACCAT GAGTTCCTCC AGCAGAGTCT GAGAAATAAA AGCTTACAGA 
     2461 TGAATGACTA TAAGATTGCC CTGCTATGTA ACGCGTATTC CACAAACTCA GAGTGTTTTA 
     2521 CGTTACCTAT GGGAGCTCTG GTAGAAACTA TTTATGGAAA TGGAATTATG AGAGTGCCTC 
     2581 TCCCTGGAAC GAGCTGTTTG GCCTCAGCAT CAGTGACTCC CTTACCGATG AACCTCCTGG 
     2641 ATTCACTGAC GGTGCATGCC AAGATGAGCC TTATTCACAG CATTGCAACC AGAGTGATAA 
     2701 AACTGGCTCA TACGAAATCC AGTGTTGCGC TGGCTCCAGC CCTAGTGGAA ACTTACAGCC 
     2761 GTTTACTGGT CTACATGGAA ATTGAGTCTT TGGGCATCAA AGGATTTATC AGTCAGCTCC 
     2821 TGCCCACTGT CTTCAAGTCC CACGCCTGGG GCATCCTGCA CACACTGCTG GAGATGTTCA 
     2881 GCCACCGAAT GCACCACATT CAGCCCCACT ACCGAGTTCA GCTCCTGAGC CATCTCCACA 
     2941 CACTGGCTGC AGTCGCACAG ACCAACCAGA ACCAGCTCCA TCTGTGTGTG GAGAGCACTG 
     3001 CACTGAGGCT CATCACAGCC CTGGGAAGCT CAGAGGTCCA GCCGCAGTTC ACGCGTTTCC 
     3061 TCAATGATCC CAAAACAGTG CTGTCCGCCG AATCTGAAGA GCTGAACCGA GCCTTGATCC 
     3121 TGACCTTAGC CAGAGCAACC CACGTCACAG ATTTTTTTAC AGGATCTGAT TCAATACAGG 
     3181 GAACTTGGTG TAAAGATATC CTTCAGACCA TCATGAACTT TACTCCTCAT AACTGGGCTT 
     3241 CACATACCCT TAGCTGTTTT CCAGCCCCTC TGCAGGCTTT CTTCAAGCAA AATAACGTGC 
     3301 CCCAGGAAAG CCGTTTTAAT CTGAAGAAGA ATGTGGAAGA AGAGTACAGG AAGTGGAAGT 
     3361 CAATGACTGA TGAAAACGAG ATCATCACCC AGTTCTCTGT GCAGGGCTTC CCTCCACTCT 
     3421 TCCTCTGTCT CCTCTGGAAG ATGCTCCTGG AGACGGATCA TATTAGTCAG ATCGGCTACA 
     3481 AGGTCCTGGA GAGGATTGGG GCCAGGGCCC TGGTGGCCCA TGTGAGAACA TTTGCAGATT 
     3541 TCCTGGTGTA TGAGTTCTCT ACGTCAGCAG GAGGTCAGCA ACTCAATAAG TGCATTGAAA 
     3601 TTCTCAACGA CATGGTGTGG AAGTACAACA TTGTCACGCT GGACAGACTC ATTCTCTGCC 
     3661 TGGCTATGCG TAGTCATGAA GGAAATGAAG CCCAGGTTTG TTATTTCATA ATTCAGTTGC 
     3721 TGTTGCTCAA ACCAAATGAC TTCAGAAACC GAGTAAGTGA CTTTGTGAAG GAGAATTCCC 
     3781 CAGAGCACTG GCTCCAGAGT GACTGGCACA CCAAGCACAT GAGCTACCAC AAGAAATATC 
     3841 CCGAGAAGCT GTATTTTGAA GGCCTGGCAG AGCAGGTTGA CCCTCCTGTA CCAATCCAGT 
     3901 CCCCTTATCT GCCCATCTAC TTTGGAAATG TGTGTCTCCG GTTCCTTCCA GTATTTGATA 
     3961 TAGTAATCCA CAGGTTTTTA GAGTTGCTTC CAGTGTCCAA ATCACTAGAG ACTCTCCTGG 
     4021 ATCACCTAGG GGGCTTATAT AAATTCCATG ACCGGCCAGT GACTTATCTG TATAATACTT 
     4081 TGCACTATTA TGAAATGTGC CTGAGAAACC GTGACCATCT CAAACGAAAA CTTGTCCATG 
     4141 CAATCATTGG CTCCCTCAAA GATAACCGGC CACAGGGCTG GTGTCTCAGT GATACCTACC 
     4201 TGAAGCATGC TATGAATGCA CGGGAGGACA ACCCCTGGGT TCCGGAGGAC TCCTACTACT 
     4261 GCAAGCTGAT TGGCCGACTG GTAGACACCA TGGCGGGCAA GTCTCCAGGA CCCTTCCCAA 
     4321 ACTGTGACTG GAGATTCAAT GAGTTTCCCA ACCCCGCTGC CCACGCTCTG CATGTGACGT 
     4381 GTGTGGAACT CATGGCCTTG GCGGTTCCAG GCAAAGATGT GGGGAATGCC CTTCTCAATG 
     4441 TCGTCCTGAA AAGCCAGCCC TTAGTGCCAA GGGAGAACAT TACAGCGTGG ATGAATGCAA 
     4501 TTGGTCTGAT CATCACGGCC CTCCCAGAGC CGTACTGGAT TGTGCTTCAT GACCGAATTG 
     4561 TGAATGTGAT CAGCAGTTCT AGCCTGACGT CAGAGACAGA GTGGGTCGGT TACCCGTTCC 
     4621 GCCTCTTCGA CTTCACCGCT TGTCACCAGT CCTACTCGGA GATGAGCTGC AGCTATACGT 
     4681 TAGCTCTAGC CCATGCCGTG TGGCACCACT CCAGCATCGG GCAGCTCTCG CTTATCCCAA 
     4741 AGTTTCTCAC TGAGGCGCTT CTCCCGGTGG TGAAGACTGA GTTCCAGTTG CTCTACGTGT 
     4801 ACCATCTTGT GGGGCCATTT CTACAGAGGT TCCAACAGGA GAGAACCCGG TGCATGATTG 
     4861 AGATTGGCGT GGCATTTTAT GACATGCTGT TGAATGTAGA CCAGTGCAGC ACGCACTTAA 
     4921 ATTACATGGA CCCCATCTGC GACTTCCTGT ATCATATGAA GTATATGTTT ACTGGTGACA 
     4981 GTGTGAAAGA GCAAGTAGAG AAGATTATCT GTAATTTAAA GCCGGCTTTA AAGCTTCGTC 
     5041 TTCGATTCAT CACGCACATT AGCAAGATGG AGCCGGCAGT GCCACCCCAG GCCCTCAACA 
     5101 GCGGGTCTCC AGCACCTCAG TCTAACCAGG TGCCAGCATC TCTGCCGGTG ACTCAGAGCG 
     5161 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5221 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5281 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5341 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5401 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5461 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5521 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5581 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5641 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5701 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5761 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5821 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5881 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5941 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6001 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6061 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6121 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6181 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6241 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6301 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6361 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6421 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6481 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6541 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6601 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6661 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6721 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6781 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6841 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6901 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6961 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7021 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7081 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7141 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7201 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7261 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7321 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7381 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7441 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7501 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7561 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7621 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7681 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7741 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7801 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7861 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7921 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7981 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8041 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8101 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8161 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8221 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8281 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8341 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8461 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8521 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8581 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8641 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8701 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8761 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8821 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8881 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8941 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9001 AAATTTAACG CGAATTTT
//