LOCUS       MR216882                8163 bp    DNA     circular  SYN 
DEFINITION  Fmnl1 (Myc-DDK-tagged) - Mouse formin-like 1 (Fmnl1), transcript variant 2
ACCESSION   NM_001077698
VERSION     NM_001077698.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4320..4411 
                     /label="Myc-DDK"  
     misc_feature    4441..5026 
                     /label="PolyA signal"  
     misc_feature    4498..4517 
                     /label="Restriction Enzyme"  
     misc_feature    5302..5974 
                     /label="ColE1"  
     misc_feature    6404..7198 
                     /label="Kan/Neo"  
     misc_feature    7233..7535 
                     /label="SV40 ori"  
     misc_feature    7621..8098 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4302..4308 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4302 
                     /label="Fmnl1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACGCG GCTGGCAGCG CCGAACAGCC CGCGGGCCCC ACCGCGTCGC 
     1081 CCCCGAAGCA GCCAGCCGTC CCCAAGCAAC CAATGCCGGC GGCCGGGGAG CTGGAGGAGA 
     1141 GGTTCACGCG GGTCTTGAAC TGTATGAACT TGCCTCCAGA CAAGGTCCAG CTGCTGAGCC 
     1201 AGTATGACAA TGAGAAGAAA TGGGAGCTCA TCTGTGACCA GGAGCGGTTT CAAGTCAAGA 
     1261 ATCCCCCTGC AGCCTACATC CAGAAGCTGA AGAGCTACCT GGATACCGGT GGGGTCAGCC 
     1321 GAAAGGTAGC ATCTGATTGG ATGTCCAACC TGGGGTTTAA GAGGCGAGTT CAGGAGTCTA 
     1381 CACAGGTGCT GCGGGAACTG GAGACTTCCC TGAGGACAAA CCACATTGGG TGGGTGCAGG 
     1441 AGTTCCTCAA TGAAGAGAAC CGGGGTCTGG ATGTCCTGCT TGAGTACCTG GCCTTTGCCC 
     1501 AGTGCTCTGT TGCATATGAC ATGGAAAGCA CAGACAGTGT GGCCTCTGGT GCAGAGAAGA 
     1561 GCAAGCCCCT GGACCAGTCT GTGGAAGACC TCAGCAAGGC ACCTCCCTCA TCTGTGCCCA 
     1621 AAAGTCGCCT GACCATCAAG CTGACCCCTG CCCACAGCAG GAAGGCGCTG AGGAATTCCC 
     1681 GAATCGTCAG CCAGAAGGAT GATGTCCACG TGTGCATCAT GTGTCTAAGA GCCATCATGA 
     1741 ACTACCAGTC CGGCTTCAGC CTTGTTATGA ACCACCCAGC CTGTGTCAAT GAGATCGCTC 
     1801 TAAGCCTTAA CAACAAGAGC CCCAGAACCA AGGCTCTGGT ACTGGAGCTG CTGGCAGCTG 
     1861 TATGTCTCGT GCGGGGAGGA CACGACATCA TCCTTGCGGC CTTTGACAAC TTCAAGGAGG 
     1921 TTTGCGGGGA GCAGCACCGA TTTGAAAAGC TAATGGAGTA TTTCCGGCAC GAAGACAGCA 
     1981 ACATTGACTT CATGGTGGCT TGCATGCAAT TCATCAACAT TGTGGTGCAC TCTGTGGAGA 
     2041 ATATGAACTT CCGTGTCTTC CTGCAATATG AGTTCACTCA CCTGGGTCTG GACCTGTACT 
     2101 TGGAGAGGCT CCGGCTCACT GAGAGTGACA AGCTGCAGGT GCAGATCCAG GCGTATCTGG 
     2161 ACAACGTTTT TGATGTGGGG ACATTGTTGG AGGAGACAGA GACGAAGAAT GCAGTGTTGG 
     2221 AGCACATGGA GGAACTGCAG GAGCAGGTGG CAACGCTGAC AGAGCGGCTT CGGGACACAG 
     2281 AGAACGACTC CATGGCCAAG ATCGCTGAAC TGGAGAAGCA GCTAAGCCAG GCTCGCAAGG 
     2341 AATTGGAGAC CCTGAGGGAG CGCTTCAGCG AGTCGACCCC CATGGGCACT TCCAGACGAA 
     2401 TCCCTGAACC TGAGAAAGTG CCTGTCCCTA CCGTGGTGCG ACCCTCGGCT CTAGAACTGA 
     2461 AGGTGGAGGA GCTTGAGGAA AAGGGGTTAA TCCGCATCCT GCGGGGGCCG GGGGACGTCG 
     2521 TTTCCATCGA GATCCTTCCA GGCGCTGCGG CGACACCAAG CGGTGACGAC GCACAAGCTC 
     2581 CGAGGGTGTC CACTGATTCC CCTAGCACAG CAGAGTCAAT TCCCGAAGCA GCATCACCAC 
     2641 CGCCTCCGCC ACCTCCACCA CCACCTCCAC TACCTAACCT CCAGTCCCAG CAGGAAGCCC 
     2701 CTCCCTCCGC ACCCCCGCTG GCCCCGCCCC TCCCAGGCTG CGCAGAGCCG CCACCTGCAC 
     2761 CACCGTTGCC TGGAGACCTG CCTCCCCCAC CTCCGCCACC CCCACTTGGT ACTGATGGAC 
     2821 CAGTGCCACC GCCACCGCCA CCGCCTCCGG GAGGGCCCCC TGATATCCTT GGAGGACAAG 
     2881 GCCCAGATAT CGGCCCAGGG GTGAAAGCCA AGAAACCCAT CCAGACCAAG TTCCGGATGC 
     2941 CACTGCTTAA CTGGGTGGCG CTGAAACCCA GCCAGATCAC AGGCACTGTC TTCACGGAGC 
     3001 TCAATGACGA GAAGGTGCTC CAGGAGCTAG ACATGAATGA CTTTGAGGAA CACTTTAAGA 
     3061 CCAAATCCCA AGGTCCCTGC CTGGATATCA GCGCTCTGAA GGGGAAGGCC TCCCAGAAGG 
     3121 CCCCCACTAA GACAATACTC ATTGAAGCCA ACCGGGCCAA GAATTTGGCT ATCACCCTGC 
     3181 GCAAGGGCAA CCTAGGAGCA GACAGAATCT GCCAGGCCAT CGAGACGTAT GACCTACAGA 
     3241 CTCTCAGCTT GGATTTCTTG GAGCTGTTGA CCCGCTTCCT GCCCACGGAC TACGAGCGTA 
     3301 GCCTCATAGC CCGATTCGAG AAGGAACAGC GGCCGATGGA AGAGCTGTCA GAGGAGGACC 
     3361 GCTTCATGCT TCGCTTCAGT CGCATCCAGA GACTTCCGGA GCGCATGAAC ACACTCACCT 
     3421 TCCTGGGCAA CTTTCCAGAC ACGGCCCAGC TGCTCATGCC GCAACTGAAC GCCATCATTG 
     3481 CAGCCTCTAT GTCTATCAAG TCCTCTGACA AGCTTCGCCA GATCCTAGAG ATTGTCCTGG 
     3541 CTTTTGGCAA CTACATGAAC AGTAGCAAGC GTGGGGCAGC CTATGGTTTC CGACTTCAGA 
     3601 GTCTGGATGC GTTGTTGGAG ATGAAGTCGA CCGACCGGAA ACAGACGTTG TTGCACTACT 
     3661 TGGTGAAGGT CATTGCTGAG AAGTACCCAC AGCTCACGGG TTTTCATAGT GATCTGCACT 
     3721 TTTTGGACAA GGCAGGCTCA GTGTCCCTGG ACAGCGTACT GGGAGACGTG CGCTCCTTGC 
     3781 AGCGAGGTCT GGAGCTGACG CAGAGAGAGT TTGTGCGGCA AGATGACTGC CTGGTGCTCA 
     3841 AGGAGTTCCT AAGGGCCAAC TCTCCCACCA TGGACAAGTT GCTGGCAGAC AGCAAGACAG 
     3901 CTCAGGAGGC CTATGAGTCT GTGGTGGAGT ACTTTGGAGA AAATCCCAAG ACCACATCCC 
     3961 CCTCCATGTT CTTTTCCCTC TTCAGTCGCT TCACCAAGGC CTACAAGAAA GCGGAACAGG 
     4021 AGGTCGAACA GTGGAAGAAG GAAGCAGCTG CTGATACCTC AGGCAGGGAA GAGCCTCCAA 
     4081 CACCCAAGTC TCCACCCAAG GCCCGGCGAC AACAGATGGA CCTCATCTCT GAGCTAAAAC 
     4141 GGAAGCAGCA GAAAGAGCCA CTCATCTACG AGAGTGACCG AGATGGGGCC ATCGAGGACA 
     4201 TCATCACAGA TCTTCGGAAC CAGCCCTACA TCCGTGCAGA CACAGGACGT CGAAGTGCTC 
     4261 GCCGGCGCCC CCCTGGACCC CCACTGCCGG TCACTACTGA CCTCGCGCTG ACGCGTACGC 
     4321 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4381 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4441 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4501 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4561 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4621 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4681 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4741 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4801 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4861 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4921 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4981 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5041 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5101 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5161 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5221 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5281 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5341 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5401 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5461 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5521 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5581 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5641 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5701 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5761 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5821 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5881 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5941 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6001 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6061 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6121 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6181 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6241 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6301 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6361 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6421 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6481 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6541 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6601 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6661 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6721 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6781 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6841 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6901 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6961 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7021 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7081 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7141 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7201 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7261 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7321 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7381 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7441 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7501 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7561 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7621 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7681 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7741 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7801 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7861 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7921 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7981 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8041 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8101 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8161 TTT
//