LOCUS       MR216718                8142 bp    DNA     circular  SYN 
DEFINITION  Ppp1r13b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 13B (Ppp1r13b)
ACCESSION   NM_011625
VERSION     NM_011625.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4299..4390 
                     /label="Myc-DDK"  
     misc_feature    4420..5005 
                     /label="PolyA signal"  
     misc_feature    4477..4496 
                     /label="Restriction Enzyme"  
     misc_feature    5281..5953 
                     /label="ColE1"  
     misc_feature    6383..7177 
                     /label="Kan/Neo"  
     misc_feature    7212..7514 
                     /label="SV40 ori"  
     misc_feature    7600..8077 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4281..4287 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4281 
                     /label="Ppp1r13b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCCGATG ATATTAACCG TGTTCTTGAG CAACAATGAA CAGATTTTAA 
     1081 CAGAAGTTCC TATAACACCA GAAACCACCT GTCGAGATGT CGTAGAATTC TGCAAGGAAC 
     1141 CTGGAGAGGG CGGTTGCCAC CTGGCTGAAG TGTGGAGAGG AAGTGAGCGT CCCATCCCCT 
     1201 ATGACCACAT GATGTATGAG CACCTGCAGA AGTGGGGGCC TCGCAGAGAA GAAGTAAAAT 
     1261 TTTTTCTTCG TCATGAGGAT TCTCCAACTG AGAGCAGTGA ACAAGGTGCC CGTCAGACCC 
     1321 AAGAGCAGAG AACTCAGAGG AGTGTAGTAA ATGTACCTGG AGAAAAGCGC ACTGAAAATG 
     1381 GGGTTGGGAA TCCACGTGTT GAACTTACCC TCTCAGAACT TCAAGATATG GCAGCCAGGC 
     1441 AGCAGCAGCA AATTGAAAAC CAGCAGCAAA TGTTGGTTGC CAAGGAACAG CGTTTACATT 
     1501 TTCTAAAGCA ACAGGAGCGC CGCCAGCAGC AGTCCGTTTC TGAAAATGAA AAGCTTCAGA 
     1561 AACTGAAAGA GCGAGTTGAA GCCCAGGAAA ACAAGCTGAA GAAGATCCGG GCCATGAGAG 
     1621 GACAAGTTGA CTACAGCAAG ATCATGAATG GCAATCTGTC TGCTGAGATA GAAAGGTTCA 
     1681 GTGCCATGTT CCAGGAGAAG AAACAGGAAG TTCAGACTGC AATCTTACGA GTTGATCAGC 
     1741 TTAGTCAGCA ACTAGAAGAT TTAAAGAAAG GAAAACTGAA TGGGTTCCAG TCATACAATG 
     1801 GCAGGTTGAC AGGGCCAGCA GCAGTGGAGT TGAAACGACT CTACCAAGAG CTGCAGATTC 
     1861 GTAACCAGCT TAATCAGGAG CAGAATTCCA AGCTCCAGCA GCAGAAGGAG CTCTTGAACA 
     1921 AGCGCAACAT GGAAGTGGCC ATGATGGATA AGCGGATCAG TGAGCTGCGG GAGCGCCTGT 
     1981 ATGGCAAGAA GATCCAGCTG AACCGTGTGA ATGGCACATC GTCACCACAG TCACCCCTGA 
     2041 GCACATCTGG CCGGGTCGCT GCTGTGGGTC CTTATATCCA GGTTCCCAGT ACTGGGGGGT 
     2101 TTCCTCTCCC AGGGGACCCT GTGAAGCCCC AGTCTCTCAC CATCGCCTCA AGTGCTGCCC 
     2161 ATGGAAGATC CAAATCTGCT AATGATGGAA ACTGGCCACC ATTAAAGCAG AATTCTGCTT 
     2221 CAGTGAAGTC AACTCAGATG ACTGGAGACT GGAAGGACTC AGGCATGGAG GGGACCCTAA 
     2281 AGCAAGGTGC CATCTCAAGC CAGCCTTTGC CTTTGTCAGC ACTGGGAGCT ACAGAAAAGC 
     2341 TGGGCATCGA GATTGGCAAA GGGCCACCTC CCATACCTGG TGTGGGCAAG CCACTGCCCC 
     2401 CAAGTTATGG GACATATCCA AGTTCTGGGC CCTTGGGCCC GGGATCAACA AGCTCTCTGG 
     2461 AGAGGAGGAA AGAAGGAAGC TTGCCCAGAC CTGGTGCAGG CCCACCGAGT CGGCAGAAAC 
     2521 CTGCCCCGCT GCCCCCAGCA AGCAACGCTC CCCAGCCTGG CTCCTCACAG CAGATTCAGC 
     2581 AGAGGATCTC AGTGCCACCA AGTCCCACAT ACCCGCCAGC GGGGCCACCT GCCTTTCCAA 
     2641 CTGGAGATGG AAAACCTGAG CTTCCACTCA CAGTGGCCAT TAGGCCTTTT CTGGCTGATA 
     2701 AAGGGTCAAG GCCACAATCT CCCAGGAAAG GACCTCAGAC AGTAAATTCA AGTTCTATAT 
     2761 ACTCCATGTA CCTCCAGCAA GCCACACCAC CTAAGAACTA CCAGCCACCA GCGCATGGCA 
     2821 CCTTAAATAA GTCAGTTAAA GCAGTGTACG GTAAGCCTGT CCTGCCGTCA GGGTCAGCAT 
     2881 CGCCATCACC CTTACCGTTT CTTCATGGGT CACTGGGCAC AGGCACGGCA CAGCCTCAGC 
     2941 CACCTTCAGA CTCTGCTGAG AAAGAGCCAG AGCAGGAAGG CCCCAGTGTA CCTGGAGAAG 
     3001 GCAGCACAGT GGAAAGCCTG CCACGGCCGC TCAGTCCCAC CAAACTCACA CCCATCGTAC 
     3061 ACTCCCCACT CCGTTATCAG AGTGATGCAG ACCTGGAAGC TCTCCGCAGG AAGCTGGCCA 
     3121 ATGCACCCCG ACCCCTGAAG AAGCGCAGCT CTATCACAGA GCCAGAGGGC CCTGGAGGGC 
     3181 CCAACATTCA AAAGTTGCTT TACCAACGTT TCAACACCTT GGCTGGTGGA ATGGAGGGTA 
     3241 CCCCTTTCTA CCAGCCCAGC CCTTCCCAGG ACTTTGTGGG CACCTTAGCT GACATGGACA 
     3301 ACGGAAACAC CAATGCCAAT GGCAACTTGG ACGAGCCTTT CCCTCCCCGG CCCACGGCCC 
     3361 CACTTCCTGA GGAGCTTGCC CCTTCCTCAG ATGCCAATGA TAATGAGTTA CCTTCCCCTG 
     3421 AGCCAGAGGA GCTCATTTGT CCCCAAACAA CTCATCAAAC TGCTGAGCCC ACTGAAGACA 
     3481 ATAACAACAA TGTGGCCCCA GTACCCTCCA CAGAACAGAT CCCAAGTCCT GTGGCTGAGG 
     3541 CTCCTTCAGA GGAGGATCAA GTCCCTCCAG CACCTCTTTC CCCCGTCATC CATCCTCCAG 
     3601 CAGCATCCGC GAGCAAACGC ACCAACCTGA AGAAGCCTAA CTCTGAACGG ACAGGGCACG 
     3661 GGCTGAGAGT GCGCTTCAAC CCCCTAGCCC TGCTTCTGGA CGCTTCTCTC GAAGGAGAGT 
     3721 TTGATCTAGT GCAGAGGATC ATCTATGAGG TGGAGGACCC TAGCAAACCC AATGACGAAG 
     3781 GGATCACCCC CCTACACAAT GCTGTCTGTG CTGGCCACCA CCATATCGTG AAGTTTCTGC 
     3841 TGGACTTTGG TGTCAATGTG AATGCTGCAG ACAGTGACGG ATGGACACCA CTGCATTGTG 
     3901 CTGCCTCTTG CAACAGTGTC CACCTCTGCA AGCAGCTGGT GGAAAGTGGA GCCGCTATCT 
     3961 TTGCCTCCAC CATCAGTGAC ATTGAGACTG CTGCAGACAA GTGTGAAGAG ATGGAAGAGG 
     4021 GATACATCCA GTGTTCCCAG TTTCTGTATG GGGTACAAGA GAAGCTGGGA GTGATGAACA 
     4081 AAGGCACCGT GTATGCTTTG TGGGACTACG AGGCCCAGAA CAGCGATGAG CTGTCCTTCC 
     4141 ATGAAGGGGA TGCCATCACC ATCCTGAGGC GCAAAGATGA AAACGAGACC GAGTGGTGGT 
     4201 GGGCTCGTCT TGGGGACCGG GAGGGCTACG TGCCCAAAAA CTTGCTGGGG TTGTATCCAC 
     4261 GGATCAAACC CCGGCAGCGA ACACTTGCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4321 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4381 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4441 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4501 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4561 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4621 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4681 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4741 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4801 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4861 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4921 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4981 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5041 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5101 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5161 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5221 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5281 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5341 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5401 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5461 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5521 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5581 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5641 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5701 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5761 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5821 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5881 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5941 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6001 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6061 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6121 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6181 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6241 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6301 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6361 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6421 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6481 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6541 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6601 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6661 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6721 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6781 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6841 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6901 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6961 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7021 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7081 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7141 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7201 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7261 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7321 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7381 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7441 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7501 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7561 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7621 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7681 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7741 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7801 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7861 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7921 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7981 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8041 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8101 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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