LOCUS       MR216717                8481 bp    DNA     circular  SYN 
DEFINITION  Gpr158 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 158 (Gpr158)
ACCESSION   NM_001004761
VERSION     NM_001004761.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="Gpr158"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGCCATG GCTTACTCTT TACTCTTCTG CCTCCTGCTC GCTCATCTGG 
     1081 GATTGGGAGA GGTTGGTGCC AGCCTCGACC CTCCGGGACG GCCAGATTCC CCTCGAGAGA 
     1141 GGACTCCGAG GGGGAAGCAG CATGGTCAGC AGCTGCCGCG AGCCTCTGCC CCAGACCCCT 
     1201 CTATTCCCTG GAGCCGCTCC ACTGACGGCA CCATCTTGGC ACAAAAACTA GCCGAGGAGG 
     1261 TGCCCGTGGA CGTGGCCTCT TACCTCTACA CTGGGGACTT CCACCAGCTG AAGAGAGCCA 
     1321 ACTGCTCAGG CCGCTACGAG TTGGCTGGCC TGCCGGGAAA GTCACCGTCC CTAGCCAGCT 
     1381 CCCATCCTTC CTTGCACGGG GCGCTGGACA CTCTGACACA CGCCACCAAC TTCCTCAACA 
     1441 TGATGTTGCA GAGCAATAAG TCTCGGGAAC AGACCGTGCA GGATGACCTG CAGTGGTACC 
     1501 AGGCTCTGGT GCGAAGTCTC CTGGAGGGTG AGCCCAGCAT CTCCCGGGCG GCCATCACCT 
     1561 TCAGCACCGA GTCGCTGTCC ACGCCCGCCC CGCAGGTCTT CCTCCAGGCC ACCCGCGAGG 
     1621 AGAGTCGCAT CCTGCTGCAG GACCTGTCCT CCTCCGCTCA CCACCTGGCT AACGCCACTC 
     1681 TAGAGACCGA GTGGTTCCAT GGGCTTCGGC GAAAGTGGAG GCCCCACTTG CACCGCCGTG 
     1741 GCTCCAATCA GGGGCCACGG GGCCTAGGCC ACAGCTGGCG GCGCAGGGAT GGGCTTGGAG 
     1801 GGGACAGGAG CCACGTCAAG TGGTCCCCAC CTTATCTGGA GTGCGAGAAT GGGAGTTACA 
     1861 AGCCTGGGTG GCTGGTCACG CTCTCTGCTG CCTTCTACGG GCTTCAACCT AACCTGGTCC 
     1921 CGGAATTCAG GGGTGTGATG AAAGTTGATA TAAGCCTTCA GAAAGTGGAC ATTGACCAAT 
     1981 GTTCGAGTGA TGGCTGGTTT TCAGGAACTC ACAAATGCCA CCTTAACAAC TCAGAGTGTA 
     2041 TGCCAATCAA AGGCCTGGGA TTCGTGCTTG GAGCCTATCA GTGCATTTGC AAAGCAGGGT 
     2101 TCTATCATCC CCGTGTCTTC TCAGTGAACA ACTTCCAGAG AAGGGGTCCA GATCACCATT 
     2161 TTTCAGGAAG CACAAAGGAT GTGTCAGAAG AAACCCACGT CTGCCTGCCC TGCAGAGAAG 
     2221 GCTGCCCCTT CTGTGCTGAT GATCGTCCAT GCTTTGTCCA GGAGGATAAA TACCTGCGGC 
     2281 TCGCTATTAT CTCTTTCCAA GCCCTGTGTA TGCTGCTGGA CTTTGTCAGC ATGCTGGTGG 
     2341 TCTACCATTT TCGCAAAGCA AAGAGCATCA GGGCATCTGG GCTCATCCTG TTGGAAACAA 
     2401 TATTGTTTGG TTCTCTGCTC CTATACTTTC CGGTCGTTAT TCTGTACTTT GAGCCAAGTA 
     2461 CATTTCGTTG TATTCTCCTA AGGTGGGCTC GTCTTCTTGG TTTTGCTACT GTTTATGGAA 
     2521 CTGTGACCCT CAAGCTTCAC AGGGTTTTGA AGGTGTTCCT TTCACGAACA GCACAAAGAA 
     2581 TCCCATACAT GACTGGTGGT CGGGTCATGA GGATGCTGGC AGTAATAGTC TTGGTTGTGT 
     2641 TTTGGTTTCT TGTTGGCTGG ACTTCTTCTA TGTGCCAGAA TTTGGAGCGG GATATTTTGC 
     2701 TTGTTGGCCA AGGGCAAACA TCTGATCACC TCACCTTCAA TATGTGCCTC ATTGACCGCT 
     2761 GGGATTACAT GACAGCAGTT GCTGAATTTT TATTCCTCTT GTGGGGCATT TATCTCTGCT 
     2821 ATGCAGTGCG GACTGTCCCT TCAGCATTTC ATGAGCCACG CTACATGGCT GTCGCAGTTC 
     2881 ACAATGAGCT CATTATCACG GCTATATTCC ATACAATTAG ATTCGTGCTC GCCTCGAGAC 
     2941 TTCAGCCCGA CTGGATGCTG ATGCTGTACT TTGCACATGC TCACTTGACT GTGACAGTCA 
     3001 CCATTGGGCT GCTTCTAATT CCAAAGTTTT CACATTCAAG CAATAATCCC CGAGATGACA 
     3061 TTGCTACGGA AGCATATGAA GATGAGCTGG ACATGGGTCG CTCTGGATCC TACCTGAACA 
     3121 GCAGTATCAA TTCAGCCTGG AGTGAACACA GCCTAGATCC AGAAGACATT CGGGATGAAC 
     3181 TGAAAAAACT CTATGCTCAG TTAGAAATAT ACAAACGTAA AAAGATGATC ACAAACAACC 
     3241 CCCACCTTCA GAAAAAACGG TGCTCCAAGA AGGGCTTAGG CCGGTCCATC ATGAGGCGCA 
     3301 TCACTGAGAT CCCAGAGACA GTCAGCAGAC AGTGCTCCAA AGAGGACAAG GAAGGCACAG 
     3361 ACCACAGTGC AGCCAAGGGG ACAGGCCTTG TCCGGAAGAA CCCTACTGAG TCCTCAGGGA 
     3421 ATACAGGGAG ACCCAAGGAA GAGTCCCTTA AAAATCGGGT CTTCTCTCTC AAGAAATCCC 
     3481 ACAGCACATA TGACCATGTG AGAGACCAAA CTGTAGAATC CAGCAGCTTG CCCATGGAGA 
     3541 GCCAGGAGGA GGAGGCCACC GAAAATTCAA CCTTGGAGTC GCTGTCAAGT AAAAAACTGA 
     3601 CACAAAAGCT CAAAGAAGAT AGTGAGGCCG AGTCCACTGA GTCAGTGCCG TTGGTCTGCA 
     3661 AGTCAGCCAG TGCTCACAAC CTCAGCTCAG AGAAGAAGCC TGGGCACCCA CGTACATCTA 
     3721 TGTTACAGAA ATCTCTCAGT GTCATTGCAA GTGCCAAGGA GAAGACACTT GGCCTGGCTG 
     3781 GCAAAACCCA GACTTTAGTT ATGGAAGACC GAGCTAAGTC CCAGAAACCA AAAGATAGAG 
     3841 AGACCATCAG GAAGTACTCA AATTCAGATA ATGTAGAGAC TATCCCAAAC TCCGGTCACA 
     3901 TGGAGGAGCC AAGAAAGCCC CAGAAATCTG GGATTATGAA ACAGCAGAGG GTCAGCCTCC 
     3961 CCACTGCCAA TCCTGACGTG AGCTCCGGTA TCACCCAGAT AAAGGACAAT TTTGATATCG 
     4021 GAGAAGTATG CCCTTGGGAG GTTTATGACC TGACCCCTGG TCCTATGCCT TCAGAGCCAA 
     4081 AAGCTCAAAA ACACGTATCA ATCGCAGCTT CTGAAGTGGA ACAAAACCCT GCTTCGTTTT 
     4141 TGAAGGAGAA GTCCTACCAC AAGTCTAAGG CAACTGAAGG TCTCTACCAA GCCAATCACA 
     4201 AGAGCATAGA CAAGACAGAG GTGTGCCCCT GGGAAATCCA CAGTCAGTCC CTTTTAGAAG 
     4261 ATGAGAATCG TCTGATTTCT AAGACTCCAG TTCTCCCAGG GAGAGCCAGA GAGGAGAATG 
     4321 GAAGCCAGCT CTACACAACC AATATGTGTG CTGGGCAATA TGAAGAACTG CCCCACAAAG 
     4381 TCGTAGCACC AAAAGTAGAG AATGAAAATC TCAACCAAAT GGGAGACCAG GAGAAACAGA 
     4441 CATCTTCCTC TGTGGATATC ATTCCTGGCT CCTGTAACTC AAGTAATAAC TCCCATCAGC 
     4501 CCTTAACATC ACGAGCCGAA GTGTGTCCCT GGGAGTTCGA GCCCCTAGAA CAACCAAATG 
     4561 CTGAAAGAAG CGTAACTTTA CCTGCCTCCT CTGCTTTAAG TGCAAATAAG ATACCAGGGC 
     4621 CTCAGAAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//