LOCUS       MR216647                9069 bp    DNA     circular  SYN 
DEFINITION  Ccnb3 (Myc-DDK-tagged) - Mouse cyclin B3 (Ccnb3)
ACCESSION   NM_183015
VERSION     NM_183015.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5226..5317 
                     /label="Myc-DDK"  
     misc_feature    5347..5932 
                     /label="PolyA signal"  
     misc_feature    5404..5423 
                     /label="Restriction Enzyme"  
     misc_feature    6208..6880 
                     /label="ColE1"  
     misc_feature    7310..8104 
                     /label="Kan/Neo"  
     misc_feature    8139..8441 
                     /label="SV40 ori"  
     misc_feature    8527..9004 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5208..5214 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5208 
                     /label="Ccnb3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACCACCA CTACTACCCA AAAGATCCAA ACTGGAGACT GAGAAGGCTC 
     1081 AGTCTAACAA GATCACACCT AGAGAGGAGC AGCAATCTGA AAAGATTGGA AAGAGTAACC 
     1141 ATGCAGCATC TTCATCTTCA TCTTCAACTC AAGGCGCAGT AAAGAGGAGA TCAGTGTTTG 
     1201 AAGATGTAAC TAATGCATCT CATAGTCAAT GTGTCCAGTC AAAGGAAGAC AATATAGAGC 
     1261 TTAAAAGCCA TGTTTCCAAA AGGACAAAGA AGGGAGTTGG TGAAGTTACT CAGAAAAAAA 
     1321 TAAAAAGTTC TAAGATGGGG CATGTTACTT CACTGTCAAA CATGGAGAAA GAGTTTATTC 
     1381 TGGATATACC CAACAAGCCT AAAACACTAA CTACTGAGGA ACCATCTGTC TTTCAAAAGA 
     1441 CATTAGTTTT AAATGAGGAG CCTGCTACTA AAGAGACATG CCTTATGAGG AAGACATTAA 
     1501 AGAGTTGTGC ATTTCATCAG GAGACACTTC TAATGGAGAA GCCACTAACC TTGCTGGTGG 
     1561 AGACTGAAGA TTACAATGAG TTTGATACAG AACTGATGAC TTCTAAAAAG AAGGATAAAC 
     1621 CTGAAGATCC AACCATCATT GAGGAGATGA CTGATTTGAA GAAGTCTGTT ATTAGGAAGG 
     1681 TGACGCTCAC CTCAAGCCCA TTATGGTTAA AGAATAAACA TGTTGTTCAA GAAGAGAAAC 
     1741 CTGTGATTCA GGAGAAGAGT TCATTCAAGA AGATATCATT AGTATCAAAT GTGGTGACTA 
     1801 CTAAAGAGAA GCCACCAGTC AAAAAGCCAC ATTTCAGGAA AAAAAAGCCT ACCACTGAGA 
     1861 TGAAGTCTTT GTTACAGGAG CCATCACTGG AAGAGAAATA TAATACTCAA GAAGATGCAA 
     1921 GCATCTTGAA GAAGCCACAG GTGTTACAAG AGAATACAAA TAACAAAGAT GCTACTTTAA 
     1981 CAGAGCCAGT TACTTTTAAA GGGAAACATA GTGCCAATGA AGCAACCCAT ACAAAGAAGC 
     2041 CATCCTCTTC TAAAAATAAT CCTGACCCTC AGGGAAAAGG CACTAATTTG AGGCCACTAA 
     2101 GAGTTCATCC AGTCACCTAT GAAAATGAGC CAATGTCCTC TAAGAAGTCA ACTACTAAGA 
     2161 AGAAAGATTC CCATTTCCAT GGGCCATCTG TACTGCCAGA CAAGCATAGC CCTCAAATGG 
     2221 AGGTGTCCAC TGTGAAAAAA TCCCTGGCTC TGCCAAATCC TACCACTGAG GAGAAGATGT 
     2281 TACATTTTCC AGTAGCTACA GTTTTGGAGA AGCAGCATAA CATGGGCGAG GCCCCTTGCC 
     2341 TGAAGAAGCC CTCACCTTTA AGGAAGCAGC AGCAGCTCCC TAAAAGAAGA CGTTTCTTCA 
     2401 GTAACTCTGC AGTACAAGAA ACAGTCATTC GCAAGCCATT GTTCTTTAAG ATGTCTACCA 
     2461 CTGAGAAAGA TCCCCCTTCC CAGTGGCCAT CTGCACTGCC AAAGAAGCAT ATCAGTCCTG 
     2521 GGGAATTGTC CAAACAGAAG AAGCAGCATG TCTCACCAAA GCATAACATG GAGGAGGACT 
     2581 CACAGTGTTG GTTAGATTCA GCTTTTAAGA AACAGCTCAG CAGGGAGGAG CCAGCCTCCA 
     2641 CCCATACGCC TTTAAAGTTA GAGATGCAGC AGGCTATTAC TAAAGAGACA GGGTTCCATT 
     2701 TGAGAAACCC ACTAGTATTA CCAACAGTCA CTTCAGAAGC AAAGTCACTG ACTAAGGAGC 
     2761 CTCCATCCTT TAGGGAACAA AATACATCTT TACTGAAAAG AAAGAGTACT ACTCACACAA 
     2821 TAACCTTACA GCAAGCACAG TCAGAGTGGC AGGAAATGAC AGATGAAGAC AGAAATTTGT 
     2881 TTTCTATAAA GCCTGGATCA CATAGGAAGG AACCTATACC TGAGTTCCTT CAGAATCCAT 
     2941 TGCCACCAAA TGAAAATTGC TTAATTTCAC AAAAACTATC CCACTCAATG CCATTTGCCT 
     3001 CACAGAAGAC CACCAGTCAA GAAAGAGCAC ATAGGAAGGA ATCGGTGGCT TCAAATGATG 
     3061 ACAAGAACTT CTTCTCGCAG GACCTATTTT CTCCTTTCAG TTCTGCTGAT GAAGATACAT 
     3121 TGAAATTCCA TAAGTCTTTG GACTTTCAAG AGCAGGTTGA TAGAAAAAAT GACTCCCACA 
     3181 AAAAAATGTT TGACTCCCAG GACAGTGTTT CCGAAGAAGA GTCCTTTTTA AGAAAGCTAT 
     3241 TTTGTAAGGA CAGATGCTCC TCTACTGAAG AATTAAGCCA AGAGAGGACT GTTGCTCTGG 
     3301 AACAGGAATT CCTCTTAATA AAAATATTGA ATGAGAATAC CAGTAGTGAT GTTGATGAGC 
     3361 CTCTTAGTCA TCAGTCACCT CATATTCAGA ACCATTCTGA CACTACGAAG GAGGCCTTGG 
     3421 AGGCCTCAGA AGCCTTGGAG GCCCCAGAAG CCTTGGAGAC CTTAGAAGCC TTGGTGGCCT 
     3481 CAGAAGACTT GGAGGAACCT CTAAACATTT TAGAAGAACT CAGCACTGAG AATATGGTTG 
     3541 CCCTCATGAA GATGTTGGTC ACAGAAGATG AGTCTACTAA GGATTCTTTT AGCGGGAATT 
     3601 ACACTGCTGC CAGGGAAGCT CATGCTGAGA AATCCTTGTC CTTGGAAGAG ACCAGCATCA 
     3661 ATGAAGCGGC AACTTTGAAG GAGTCCTTAT CTTCACAGGA GAAGCACAGA GCTGAGCTGG 
     3721 TGACTGTTCT GAAGGAGCTA CTTGTTTTAA TGAAGAATCC CAGTCTGAAG AGGGTGGCCT 
     3781 TAGCGTTTCA GGAGAATCCA TCTAATAACG TAGAGACTCT CTTGAGGGAA GTATTGGCAT 
     3841 TGGTGGAGAA CTCTACAGCT GACGAATCTA CTCTGCAGGA GAAACCTAGC ACTAAAACAG 
     3901 ATGTTACCCC AAAGGAACTA TTGGCCTTGG AAGAGAATTC CAGCAATAAA AAGGCAAACC 
     3961 CCATGGACTC TTTATCCTTT GATCATAAGC CTGACACTGA GATGGGTGAA ATAGCCAGAA 
     4021 TGGTATTAAC TGATGAAGAG TACAACATAG ATACCCTGTA TGAGAGAGTA TTAGCACTGA 
     4081 GTCAGGGACT CATTGCTGCA GACCAGTTGT CCTTTACAGA TCTACAGAAT TTTGAAGAGA 
     4141 CTAAAATTGT TGATGAAGAG GAATTTTTCA AATCATTCCT TGTCTTTGAA AATAAGAATA 
     4201 GCCCTAATAT GTCCAGCAAT GCTTTTGAAT CCAGAACAGA CAATTCCAGT GCTATCATGC 
     4261 CAAGTTCAAA GGCATTCAAT CCTGTCGAGA ACTCCAACCC ATATGTGTCT AGTTCCAAAA 
     4321 GCTTTAAGTC AACTCTGGGT GCAAAAGAAA CTGAGATAAC CATACAGGAT GACAGTGACA 
     4381 GCCTGGAAAG AATTGAAAAG GAAGGCCAAG ATCCATTATT GAACACTATA TATGCCAAAG 
     4441 ACGTCTTCAA TTACTTGAAA GAAAGAGAGG AAAAGTTCCT AGTTCAGAAA TACATGGATG 
     4501 GGCAGATGGA ACTCACCAGT GATATGAGAG CCATTCTTGT GGACTGGTTG GTGGAGATAC 
     4561 AGGGATCCTT TCAGATGACC CATGAGACCC TGTATTTGGC AGTGAAGATA ATGGATCTCT 
     4621 ACCTAATGAA GGCACAATGC AAGAAGAATC ATCTACAACT CCTTGGTTCC ACTACTTACA 
     4681 TGATTGCAGC CAAATTTGAG GAATCCTATC CCCCTTCTCT GTCAGAGTTC CTGTTCATTT 
     4741 GTGAAGATAT GTATGAAAAG AGTGATATGG TATCTTTGGA AAGCAGCATC CTGCAAACTC 
     4801 TCAACTTTGA CATCAATATT CCCACAGCAT ATAATTTTCT GCGTAGATAT GCTTCGTGCA 
     4861 TCCATGCTAG CATGAAGACA CTAACCTTAT CCCGCTTCAT CTGTGAGATG ACCCTGCAAG 
     4921 AATATGAGTA TATTGAAGAG AGGCCTTCAA AGCTAGCTGC AGCCTCTTTC ATCTTGGCCC 
     4981 TCTATATGAG GAATCTCAGC AATTGTGTTC CTACACTGGA ATATTTCACT GGGTACAAGA 
     5041 TGGCAGAACT CCATATCTTG GTCAGGAAGT TAAACCATTT GCTGAACTTC CGATCCCATA 
     5101 GTATCCTCAA GAATGTGTTT GAGAAGTATT CTGAAGAGAC CTACTTTGAA GTCGCCAAAA 
     5161 TCCCTCCTTT GAGCAAGCAA GATCTAGAGA ATCTTTTGAA TTGTGCATTA TTCCACACGC 
     5221 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5281 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5341 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5401 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5461 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5521 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5581 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5641 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5701 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5761 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5821 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5881 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5941 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6001 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6061 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6121 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6181 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6241 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6301 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6361 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6421 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6481 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6541 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6601 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6661 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6721 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6781 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6841 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6901 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6961 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7021 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7081 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7141 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7201 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7261 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7321 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7381 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7441 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7501 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7561 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7621 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7681 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7741 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7801 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7861 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7921 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7981 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8041 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8101 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8161 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8221 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8281 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8341 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8401 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8461 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8521 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8581 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8641 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8701 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8761 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8821 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8881 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8941 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9001 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9061 GCGAATTTT
//