LOCUS       MR216613                8445 bp    DNA     circular  SYN 
DEFINITION  Pum1 (Myc-DDK-tagged) - Mouse pumilio 1 (Drosophila) (Pum1), transcript variant 3
ACCESSION   NM_001159604
VERSION     NM_001159604.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4602..4693 
                     /label="Myc-DDK"  
     misc_feature    4723..5308 
                     /label="PolyA signal"  
     misc_feature    4780..4799 
                     /label="Restriction Enzyme"  
     misc_feature    5584..6256 
                     /label="ColE1"  
     misc_feature    6686..7480 
                     /label="Kan/Neo"  
     misc_feature    7515..7817 
                     /label="SV40 ori"  
     misc_feature    7903..8380 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4584..4590 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4584 
                     /label="Pum1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGTTGCA TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT 
     1081 TCAGCCCCCA CCTGAAACAT CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT 
     1141 TGACATCTGG AACAGGGTCG CAAGCGCAGC CACAGCCAGC TGCAAATCAG GCTCTTGCAG 
     1201 CTGGAACACA CTCCAGCCCT GTCCCAGGAT CCATAGGAGT TGCAGGCCGT TCCCAGGACG 
     1261 ACGCTATGGT GGACTACTTC TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGAA 
     1321 GTGGTGGAGG CGGCTATAAT ACTAGCAAAC ATCGATGGCC TACAGGGGAT AACATTCATG 
     1381 CAGAACACCA GGTACGCTCT ATGGATGAAT TGAATCATGA TTTTCAAGCA CTTGCTCTGG 
     1441 AAGGGAGAGC TATGGGCGAG CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT 
     1501 CCAGCAAAGA TGGACCAAAA GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG 
     1561 GAACATCAGA TCATTCAGTT TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT 
     1621 TCCATGTGAA CAGTGAGGTC AATTCTGTAC TGTCCCCACG GTCGGAGAGT GGGGGACTAG 
     1681 GCGTTAGCAT GGTGGAGTAT GTGTTGAGCT CATCCCCGGG CGACTCCTGT CTAAGAAAAG 
     1741 GAGGATTTGG CCCAAGGGAT GCAGACAGTG ATGAAAACGA CAAAGGTGAA AAGAAGAACA 
     1801 AGGGTACGTT TGATGGAGAT AAGCTAGGAG ATTTGAAGGA GGAGGGAGAT GTGATGGACA 
     1861 AGACCAATGG TTTGCCAGTG CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA 
     1921 CCCCTGGTAA TTGCCAGAAC TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG 
     1981 GTTCTGAGGG CTTAGCCCAG CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT 
     2041 CCAACATGGA GTCCCAGAGT GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC 
     2101 TGCAGTTTGA TTATTCAGGC ACGCAGGTAC CTGTGGACTC TGCAGCAGCA ACTGTGGGAC 
     2161 TTTTTGACTA CAATTCTCAA CAACAGCTAT TCCAAAGACC CAATGCACTT GCTGTTCAGC 
     2221 AGTTGACAGC TGCCCAGCAG CAGCAGTATG CACTTGCAGC AGCCCACCAG CCTCACATCG 
     2281 GTTTAGCTCC CGCTGCGTTT GTCCCCAACC CATACATCAT CAGCGCTGCT CCCCCAGGGA 
     2341 CGGACCCCTA CACAGCTGGA TTGGCTGCAG CAGCGACACT CGGCCCAGCT GTGGTCCCTC 
     2401 ACCAATATTA TGGAGTCACG CCATGGGGTG TCTACCCTGC CAGTCTCTTC CAGCAGCAGG 
     2461 CAGCTGCAGC TGCAGCAGCT ACAAACTCTG CTACTCAGCA GAGCGCTCCG CAGGCCCAGC 
     2521 AGGGACAGCA GCAGGTTCTT CGTGGAGGAG CTAGCCAACG TCCTTTGACC CCAAATCAGA 
     2581 ACCAGCAGGG ACAACAAACA GATCCCCTTG TAGCAGCTGC AGCAGTGAAT TCTGCTCTTG 
     2641 CCTTTGGACA AGGATTGGCT GCAGGTATGC CAGGTTATCC AGTCTTGGCA CCTGCTGCTT 
     2701 ACTATGACCA AACTGGTGCC CTTGTGGTGA ATGCAGGGGC AAGAAATGGC CTCGGAGCTC 
     2761 CAGTCCGGCT TGTTGCTCCA GCCCCAGTCA TCATTAGCTC CTCAGCTGCA CAAGCAGCTG 
     2821 TTGCAGCGGC TGCAGCGTCA GCAAACGGAG CAGCTGGTGG TCTTGCCGGA ACTACAAATG 
     2881 GACCATTTCG ACCTTTAGGC ACCCAGCAGC CCCAGCCCCA GCCTCAGCAG CAGCCCAGTA 
     2941 ACAATCTGGC TTCCAGCTCT TTCTATGGCA ACAACTCTCT GAGCAGTAAC TCCCAGAGCA 
     3001 GCTCCCTCTT CTCTCAGGGC TCTGCACAGC CTGCCAACAC GTCCTTGGGA TTCGGAAGCA 
     3061 GCAGTTCCCT TGGTGCCACC CTGGGATCGG CCCTTGGAGG GTTTGGAACA GCAGTTGCAA 
     3121 ACTCCAACAC TGGCAGTGGC TCCCGCCGTG ACTCCCTGAC TGGCAGCAGT GACCTTTATA 
     3181 AGAGGACATC GAGCAGCTTG GCCCCCATTG GACACAGTTT TTATAGCAGC CTTAGCTATT 
     3241 CCTCCTCTCC TGGACCCGTG GGCATGCCTC TCCCTAGTCA GGGACCAGGA CATTCACAGA 
     3301 CACCACCTCC TTCCCTCTCT TCACATGGAT CCTCTTCAAG CTTAAACCTG GGAGGACTCA 
     3361 CAAACGGCAG TGGAAGATAC ATCTCTGCTG CTCCCGGTGC CGAAGCCAAG TACCGCAGTG 
     3421 CAAGCAGTGC CTCCAGCCTT TTCAGCCCCA GCAGCACCCT TTTTTCTTCC TCTCGTTTGC 
     3481 GATATGGAAT GTCTGATGTT ATGCCCTCTG GCAGGAGCAG ACTTCTGGAA GATTTTCGAA 
     3541 ACAACCGGTA CCCTAATTTA CAACTGCGGG AGATTGCTGG GCATATAATG GAATTTTCTC 
     3601 AAGACCAGCA TGGCTCTAGA TTCATTCAGC TGAAACTAGA GCGTGCCACA GCAGCTGAGC 
     3661 GCCAGCTTGT CTTCAATGAA ATCCTCCAGG CTGCGTACCA ACTTATGGTG GATGTGTTTG 
     3721 GAAATTACGT CATTCAGAAG TTTTTTGAAT TTGGCAGTCA TGAACAGAAG CTGGCTCTGG 
     3781 CTGAACGGAT TCGAGGCCAT GTCCTATCAT TGGCACTGCA GATGTACGGC TGCCGAGTCA 
     3841 TCCAGAAAGC TTTGGAGTTT ATTCCCTCAG ACCAGCAGGT AATTAACGAG ATGGTGCGGG 
     3901 AGCTAGATGG CCATGTCCTG AAGTGTGTGA AAGACCAGAA CGGCAACCAT GTGGTTCAGA 
     3961 AGTGCATTGA GTGTGTACAG CCCCAGTCTT TGCAGTTTAT CATCGATGCA TTCAAGGGGC 
     4021 AGGTGTTTGC TTTGTCTACG CACCCGTACG GCTGTAGGGT GATCCAGAGG ATCCTAGAGC 
     4081 ACTGTCTCCC TGACCAGACC CTCCCCATCC TGGAGGAGCT TCACCAGCAC ACAGAGCAGC 
     4141 TTGTGCAGGA TCAGTATGGA AATTATGTAA TCCAACATGT ACTGGAGCAT GGTCGTCCTG 
     4201 AGGATAAAAG CAAAATTGTA GCAGAGATCC GAGGCAATGT ACTTGTATTG AGTCAGCACA 
     4261 AGTTTGCAAG CAATGTTGTG GAGAAGTGTG TTACTCACGC CTCACGTACA GAGCGTGCTG 
     4321 TGCTCATTGA TGAGGTGTGC ACCATGAACG ACGGTCCCCA CAGTGCCTTA TACACCATGA 
     4381 TGAAGGATCA GTATGCTAAC TATGTGGTCC AGAAGATGAT CGATGTGGCT GAGCCGGGTC 
     4441 AGCGGAAGAT TGTCATGCAC AAGATCCGGC CACACATTGC GACGCTTCGA AAGTACACCT 
     4501 ATGGCAAGCA CATCCTGGCC AAGCTCGAGA AGTACTACAT GAAGAATGGC GTGGACTTGG 
     4561 GGCCCATTTG TGGTCCCCCT AATGGTATCA TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4621 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4681 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4741 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4801 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4861 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4921 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4981 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5041 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5101 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5161 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5221 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5281 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5341 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5401 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5461 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5521 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5581 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5641 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5701 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5761 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5821 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5881 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5941 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6001 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6061 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6121 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6181 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6241 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6301 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6361 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6421 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6481 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6541 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6601 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6661 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6721 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6781 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6841 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6901 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6961 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7021 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7081 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7141 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7201 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7261 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7321 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7381 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7441 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7501 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7561 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7621 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7681 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7801 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7861 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7921 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7981 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8041 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8101 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8161 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8221 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8281 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8341 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8401 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//