LOCUS       MR216598                8439 bp    DNA     circular  SYN 
DEFINITION  Pum1 (Myc-DDK-tagged) - Mouse pumilio 1 (Drosophila) (Pum1), transcript variant 1
ACCESSION   NM_030722
VERSION     NM_030722.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4596..4687 
                     /label="Myc-DDK"  
     misc_feature    4717..5302 
                     /label="PolyA signal"  
     misc_feature    4774..4793 
                     /label="Restriction Enzyme"  
     misc_feature    5578..6250 
                     /label="ColE1"  
     misc_feature    6680..7474 
                     /label="Kan/Neo"  
     misc_feature    7509..7811 
                     /label="SV40 ori"  
     misc_feature    7897..8374 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4578..4584 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4578 
                     /label="Pum1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGTTGCA TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT 
     1081 TCAGCCCCCA CCTGAAACAT CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT 
     1141 TGACATCTGG AACAGGGTCG CAAGCGCAGC CACAGCCAGC TGCAAATCAG GCTCTTGCAG 
     1201 CTGGAACACA CTCCAGCCCT GTCCCAGGAT CCATAGGAGT TGCAGGCCGT TCCCAGGACG 
     1261 ACGCTATGGT GGACTACTTC TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGAA 
     1321 GTGGTGGAGG CGGCTATAAT ACTAGCAAAC ATCGATGGCC TACAGGGGAT AACATTCATG 
     1381 CAGAACACCA GGTACGCTCT ATGGATGAAT TGAATCATGA TTTTCAAGCA CTTGCTCTGG 
     1441 AAGGGAGAGC TATGGGCGAG CAGCTCTTGC CAGGTAAAAA GTTTTGGGAA ACAGATGAAT 
     1501 CCAGCAAAGA TGGACCAAAA GGAATATTCC TGGGTGATCA ATGGCGAGAC AGTGCCTGGG 
     1561 GAACATCAGA TCATTCAGTT TCCCAGCCAA TCATGGTGCA GAGAAGACCT GGTCAGAGTT 
     1621 TCCATGTGAA CAGTGAGGTC AATTCTGTAC TGTCCCCACG GTCGGAGAGT GGGGGACTAG 
     1681 GCGTTAGCAT GGTGGAGTAT GTGTTGAGCT CATCCCCGGG CGACTCCTGT CTAAGAAAAG 
     1741 GAGGATTTGG CCCAAGGGAT GCAGACAGTG ATGAAAACGA CAAAGGTGAA AAGAAGAACA 
     1801 AGGGTACGTT TGATGGAGAT AAGCTAGGAG ATTTGAAGGA GGAGGGAGAT GTGATGGACA 
     1861 AGACCAATGG TTTGCCAGTG CAGAATGGGA TTGATGCAGA CGTCAAAGAT TTTAGCCGTA 
     1921 CCCCTGGTAA TTGCCAGAAC TCTGCTAATG AAGTGGATCT TCTGGGTCCA AACCAGAATG 
     1981 GTTCTGAGGG CTTAGCCCAG CTGACCAGCA CCAATGGTGC CAAGCCTGTG GAGGATTTCT 
     2041 CCAACATGGA GTCCCAGAGT GTCCCCTTGG ACCCCATGGA ACATGTGGGC ATGGAGCCTC 
     2101 TGCAGTTTGA TTATTCAGGC ACGCAGGTAC CTGTGGACTC TGCAGCAGCA ACTGTGGGAC 
     2161 TTTTTGACTA CAATTCTCAA CAACAGCTAT TCCAAAGACC CAATGCACTT GCTGTTCAGC 
     2221 AGTTGACAGC TGCCCAGCAG CAGCAGTATG CACTTGCAGC AGCCCACCAG CCTCACATCG 
     2281 GTTTAGCTCC CGCTGCGTTT GTCCCCAACC CATACATCAT CAGCGCTGCT CCCCCAGGGA 
     2341 CGGACCCCTA CACAGCTGGA TTGGCTGCAG CAGCGACACT CGGCCCAGCT GTGGTCCCTC 
     2401 ACCAATATTA TGGAGTCACG CCATGGGGTG TCTACCCTGC CAGTCTCTTC CAGCAGCAGG 
     2461 CAGCTGCAGC TGCAGCAGCT ACAAACTCTG CTACTCAGCA GAGCGCTCCG CAGGCCCAGC 
     2521 AGGGACAGCA GCAGGTTCTT CGTGGAGGAG CTAGCCAACG TCCTTTGACC CCAAATCAGA 
     2581 ACCAGCAGGG ACAACAAACA GATCCCCTTG TAGCAGCTGC AGCAGTGAAT TCTGCTCTTG 
     2641 CCTTTGGACA AGGATTGGCT GCAGGTATGC CAGGTTATCC AGTCTTGGCA CCTGCTGCTT 
     2701 ACTATGACCA AACTGGTGCC CTTGTGGTGA ATGCAGGGGC AAGAAATGGC CTCGGAGCTC 
     2761 CAGTCCGGCT TGTTGCTCCA GCCCCAGTCA TCATTAGCTC CTCAGCTGCA CAAGCAGCTG 
     2821 TTGCAGCGGC TGCAGCGTCA GCAAACGGAG CAGCTGGTGG TCTTGCCGGA ACTACAAATG 
     2881 GACCATTTCG ACCTTTAGGC ACCCAGCAGC CCCAGCCCCA GCCTCAGCAG CAGCCCAGTA 
     2941 ACAATCTGGC TTCCAGCTCT TTCTATGGCA ACAACTCTCT GAGCAGTAAC TCCCAGAGCA 
     3001 GCTCCCTCTT CTCTCAGGGC TCTGCACAGC CTGCCAACAC GTCCTTGGGA TTCGGAAGCA 
     3061 GCAGTTCCCT TGGTGCCACC CTGGGATCGG CCCTTGGAGG GTTTGGAACA GCAGTTGCAA 
     3121 ACTCCAACAC TGGCAGTGGC TCCCGCCGTG ACTCCCTGAC TGGCAGCAGT GACCTTTATA 
     3181 AGAGGACATC GAGCAGCTTG GCCCCCATTG GACACAGTTT TTATAGCAGC CTTAGCTATT 
     3241 CCTCCTCTCC TGGACCCGTG GGCATGCCTC TCCCTAGTCA GGGACCAGGA CATTCACAGA 
     3301 CACCACCTCC TTCCCTCTCT TCACATGGAT CCTCTTCAAG CTTAAACCTG GGAGGACTCA 
     3361 CAAACGGCAG TGGAAGATAC ATCTCTGCTG CTCCCGGTGC CGAAGCCAAG TACCGCAGTG 
     3421 CAAGCAGTGC CTCCAGCCTT TTCAGCCCCA GCAGCACCCT TTTTTCTTCC TCTCGTTTGC 
     3481 GATATGGAAT GTCTGATGTT ATGCCCTCTG GCAGGAGCAG ACTTCTGGAA GATTTTCGAA 
     3541 ACAACCGGTA CCCTAATTTA CAACTGCGGG AGATTGCTGG GCATATAATG GAATTTTCTC 
     3601 AAGACCAGCA TGGCTCTAGA TTCATTCAGC TGAAACTAGA GCGTGCCACA GCAGCTGAGC 
     3661 GCCAGCTTGT CTTCAATGAA ATCCTCCAGG CTGCGTACCA ACTTATGGTG GATGTGTTTG 
     3721 GAAATTACGT CATTCAGAAG TTTTTTGAAT TTGGCAGTCA TGAACAGAAG CTGGCTCTGG 
     3781 CTGAACGGAT TCGAGGCCAT GTCCTATCAT TGGCACTGCA GATGTACGGC TGCCGAGTCA 
     3841 TCCAGAAAGC TTTGGAGTTT ATTCCCTCAG ACCAGCAGAA CGAGATGGTG CGGGAGCTAG 
     3901 ATGGCCATGT CCTGAAGTGT GTGAAAGACC AGAACGGCAA CCATGTGGTT CAGAAGTGCA 
     3961 TTGAGTGTGT ACAGCCCCAG TCTTTGCAGT TTATCATCGA TGCATTCAAG GGGCAGGTGT 
     4021 TTGCTTTGTC TACGCACCCG TACGGCTGTA GGGTGATCCA GAGGATCCTA GAGCACTGTC 
     4081 TCCCTGACCA GACCCTCCCC ATCCTGGAGG AGCTTCACCA GCACACAGAG CAGCTTGTGC 
     4141 AGGATCAGTA TGGAAATTAT GTAATCCAAC ATGTACTGGA GCATGGTCGT CCTGAGGATA 
     4201 AAAGCAAAAT TGTAGCAGAG ATCCGAGGCA ATGTACTTGT ATTGAGTCAG CACAAGTTTG 
     4261 CAAGCAATGT TGTGGAGAAG TGTGTTACTC ACGCCTCACG TACAGAGCGT GCTGTGCTCA 
     4321 TTGATGAGGT GTGCACCATG AACGACGGTC CCCACAGTGC CTTATACACC ATGATGAAGG 
     4381 ATCAGTATGC TAACTATGTG GTCCAGAAGA TGATCGATGT GGCTGAGCCG GGTCAGCGGA 
     4441 AGATTGTCAT GCACAAGATC CGGCCACACA TTGCGACGCT TCGAAAGTAC ACCTATGGCA 
     4501 AGCACATCCT GGCCAAGCTC GAGAAGTACT ACATGAAGAA TGGCGTGGAC TTGGGGCCCA 
     4561 TTTGTGGTCC CCCTAATGGT ATCATCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4621 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4681 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4741 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4801 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4861 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4921 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4981 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5041 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5101 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5161 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5221 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5281 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5341 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5401 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5461 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5521 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5581 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5641 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5701 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5761 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5821 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5881 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5941 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6001 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6061 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6121 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6181 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6241 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6301 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6361 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6421 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6481 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6541 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6601 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6661 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6721 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6781 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6841 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6901 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6961 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7021 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7081 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7141 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7201 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7261 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7321 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7381 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7441 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7501 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7561 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7621 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7681 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7861 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7921 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7981 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8041 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8101 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8161 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8221 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8281 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8341 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8401 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//