LOCUS       MR216561                8574 bp    DNA     circular  SYN 
DEFINITION  Stag2 (Myc-DDK-tagged) - Mouse stromal antigen 2 (Stag2), transcript variant 2
ACCESSION   NM_001077712
VERSION     NM_001077712.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4731..4822 
                     /label="Myc-DDK"  
     misc_feature    4852..5437 
                     /label="PolyA signal"  
     misc_feature    4909..4928 
                     /label="Restriction Enzyme"  
     misc_feature    5713..6385 
                     /label="ColE1"  
     misc_feature    6815..7609 
                     /label="Kan/Neo"  
     misc_feature    7644..7946 
                     /label="SV40 ori"  
     misc_feature    8032..8509 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4713..4719 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4713 
                     /label="Stag2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATAGCAGCT CCAGAAATAC CAACTGATTT TAATCTACTG CAGGAGTCAG 
     1081 AGACACACTT TTCTTCTGAC ACAGATTTTG AAGACATTGA AGGAAAAAAT CAGAAGCAAG 
     1141 GCAAAGGCAA AACTTGTAAA AAAGGCAAAA AGGGTCCAGC AGAAAAGGGT AAAAGTGGAA 
     1201 ATGGAGGAGG AAAACCTCCT TCTGGCTCAA ACCGAATGAA CGGTCATCAT CAACAGAATG 
     1261 GAGTTGAAAA CATGATGTTG TTCGAAGTTG TTAAAATGGG CAAGAGTGCT ATGCAGTCGG 
     1321 TGGTAGATGA CTGGATCGAG TCCTACAAGC ATGACCGGGA CATAGCGCTT CTTGACCTTA 
     1381 TCAATTTCTT TATTCAGTGC TCAGGCTGCA AAGGAGTCGT CACAGCAGAG ATGTTCAGAC 
     1441 ATATGCAGAA CTCTGAGATA ATTCGAAAAA TGACAGAAGA ATTTGACGAG GATAGTGGGG 
     1501 ATTACCCACT TACCATGGCT GGTCCTCAGT GGAAGAAGTT CAAGTCCAGC TTCTGTGAGT 
     1561 TCATTGGTGT GTTAGTACGG CAGTGTCAAT ACAGTATCAT ATATGATGAG TACATGATGG 
     1621 ATACTGTCAT TTCACTTCTT ACTGGATTGT CTGACTCCCA AGTCAGAGCA TTTCGACATA 
     1681 CAAGCACCCT GGCAGCTATG AAGTTGATGA CTGCTTTGGT AAATGTGGCA CTAAATCTTA 
     1741 GCATTAATAT GGATAACACA CAAAGACAGT ACGAGGCAGA ACGGAATAAA ATGATTGGAA 
     1801 AACGAGCTAA CGAAAGGCTA GAACTCCTGT TACAAAAGCG TAAAGAGCTT CAGGAAAATC 
     1861 AAGATGAGAT AGAAAATATG ATGAATGCAA TATTTAAAGG AGTGTTTGTA CATAGATACA 
     1921 GGGATGCAAT AGCTGAAATC AGAGCTATAT GCATTGAAGA GATTGGCATT TGGATGAAGA 
     1981 TGTATAGTGA TGCCTTCCTT AATGACAGCT ATTTAAAATA TGTTGGTTGG ACTATGCATG 
     2041 ATAAGCAAGG TGAAGTCAGA CTCAAATGTC TTACTGCTTT ACAAGGGCTT TACTATAACA 
     2101 AAGAACTTAA TTCCAAACTG GAACTCTTTA CCAGTCGATT CAAGGATAGA ATTGTGTCTA 
     2161 TGACCCTTGA CAAAGAATAT GATGTTGCAG TACAAGCAAT AAAGTTACTC ACTCTTGTTT 
     2221 TACAGAGTAG TGAAGAAGTT CTAACTGCAG AAGATTGTGA AAATGTCTAT CATCTGGTTT 
     2281 ATTCAGCTCA CCGGCCAGTA GCAGTTGCAG CTGGAGAATT TCTCTACAAA AAGCTCTTCA 
     2341 GCCGCAGAGA CCCAGAAGAG GATGGGCTGA TGAAAAGAAG GGGAAGGCAA GGCCCGAATG 
     2401 CCAACCTTGT GAAGACTTTG GTGTTTTTCT TTTTGGAAAG TGAGTTACAC GAGCATGCAG 
     2461 CTTACCTTGT GGACAGCATG TGGGATTGTG CTACTGAGCT CCTGAAAGAC TGGGAATGTA 
     2521 TGAATAGCTT GTTATTAGAA GAGCCTCTTA GTGGAGAGGA AGCTCTAACA GACAGACAAG 
     2581 AGAGTGCTTT GATTGAAATA ATGCTTTGTA CTATTAGACA AGCAGCTGAA TGTCATCCTC 
     2641 CCGTGGGAAG AGGGACAGGA AAAAGGGTGC TGACAGCAAA GGAGAAGAAG ACACAGTTGG 
     2701 ATGACAGAAC AAGAATCACT GAGCTGTTTG CTGTGGCCCT TCCGCAGTTA TTAGCTAAAT 
     2761 ACTCTGTAGA TGCAGAAAAG GTGACCAATT TGTTGCAGTT ACCTCAGTAC TTTGATTTGG 
     2821 AGATATACAC CACTGGACGA TTAGAAAAGC ATTTGGATGC CTTATTGCGC CAGATCCGAA 
     2881 ATATTGTAGA GAAGCACACA GACACAGATG TTTTGGAAGC ATGTTCTAAA ACTTACCATG 
     2941 CATTATGTAA CGAAGAGTTC ACCATCTTCA ACAGAGTAGA TATTTCAAGA AGTCAGCTGA 
     3001 TTGATGAACT GGCAGATAAA TTTAACCGGC TTCTTGAAGA TTTTCTGCAA GAGGGAGAAG 
     3061 AACCTGATGA AGATGACGCA TATCAGGTAT TGTCAACATT GAAGAGGATC ACTGCTTTTC 
     3121 ATAATGCTCA TGATCTTTCA AAGTGGGATT TGTTCGCTTG TAATTACAAA CTCTTGAAAA 
     3181 CCGGAATCGA AAATGGAGAT ATGCCTGAGC AGATTGTTAT TCACGCACTG CAGTGTGCTC 
     3241 ACTATGTAAT CCTTTGGCAG CTTGCTAAGA TCACTGAAAG CACGTCTACA AAGGAGGACT 
     3301 TACTGCGCTT AAAGAAACAG ATGAGGGTAT TTTGCCAGAT ATGTCAGCAT TACTTGACCA 
     3361 ACGTGAATAC TACTGTTAAA GAACAGGCCT TCACTATTCT GTGTGACATT TTGATGATCT 
     3421 TCAGTCACCA GATTATGTCT GGAGGGCGTG ACATGTTAGA GCCATTAGTT TATACTCCTG 
     3481 ATTCTTCATT GCAGTCTGAG TTGCTCAGCT TTATTTTGGA TCATGTCTTC ATTGAACAGG 
     3541 ATGATGATAG TAATAGTGCA GATGGTCAGC AAGAGGATGA AGCCAGCAAA ATTGAAGCTT 
     3601 TGCACAAGAG GAGAAATTTA CTTGCAGCAT TTTGTAAGCT AATTGTATAT ACCGTGGTGG 
     3661 AGATGAACAC AGCTGCAGAT ATCTTCAAGC AGTATATGAA GTATTATAAT GATTATGGCG 
     3721 ATATCATCAA AGAAACAATG AGTAAAACAA GGCAGATTGA CAAAATTCAG TGTGCTAAGA 
     3781 CCCTTATTCT CAGTTTGCAG CAGCTTTTTA ATGAGATGAT ACAAGAGAAT GGCTATAATT 
     3841 TTGATAGATC ATCCTCAACA TTTAGTGGCA TAAAAGAACT TGCTCGGCGT TTTGCTTTAA 
     3901 CTTTTGGACT TGATCAATTG AAAACCAGAG AAGCCATTGC CATGCTCCAC AAAGATGGTA 
     3961 TAGAATTTGC TTTTAAGGAG CCTAATCCAC AAGGGGAGAG CCATCCACCT TTAAACTTGG 
     4021 CATTTCTTGA TATTCTCAGT GAATTTTCTT CTAAACTGCT CCGACAGGAT AAAAGAACAG 
     4081 TGTATGTTTA TTTGGAAAAG TTCATGACCT TTCAGATGTC ACTCCGAAGA GAAGATGTAT 
     4141 GGCTTCCTCT GATGTCTTAC CGAAATTCTT TGCTAGCTGG TGGTGATGAT GATACTATGT 
     4201 CAGTCATTAG TGGAATGAGC AGTCGGGGAT CAACTGTACG GAGTAAAAAA TCAAAACCAT 
     4261 CTACAGGAAA GCGGAAAGTG GTTGAAGGCA TGCAACTAGC ACTCCCTGAA GAAAGCAGTA 
     4321 GCAGTGACAG TATGTGGTTG AGCAGAGAGC AAACACTGCA CACCCCTGTC ATGATGCAGA 
     4381 CACCACAGCT CACCTCCACT ATTATGAGAG AACCGAAAAG GTTACGGCCT GAAGATAGCT 
     4441 TCATGAGTGT CTATCCGATG CAGGCAGAAC ACCATCAAAC GCCTCTGGAT TATAATCGGC 
     4501 GTGGTACAAG CCTAATGGAA GATGATGAAG AGCCAATTGT TGAAGATGTT ATGATGTCCT 
     4561 CAGAAGGAAG GATTGAGGAT CTTAATGAGG GAATGGACTT TGACACCATG GATATAGACT 
     4621 TGCCACCATC AAAGAATAGA AGAGAGAGAA CAGAACTGAA GCCCGATTTC TTTGATCCAG 
     4681 CTTCAATTAT GGATGAATCA GTTCTTGGGG TGTCAATGTT TACGCGTACG CGGCCGCTCG 
     4741 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4801 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4861 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4921 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4981 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5041 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5101 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5161 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5221 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5281 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5341 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5401 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5461 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5521 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5581 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5641 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5701 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5761 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5821 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5881 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5941 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6001 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6061 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6121 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6181 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6241 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6301 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6361 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6421 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6481 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6541 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6601 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6661 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6721 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6781 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6841 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6901 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6961 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7021 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7081 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7141 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7201 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7261 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7321 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7381 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7441 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7501 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7561 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7621 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7681 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7741 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7801 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7981 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8041 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8101 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8161 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8221 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8281 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8341 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8401 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8461 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8521 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//