LOCUS       MR216552                9156 bp    DNA     circular  SYN 
DEFINITION  Nphp4 (Myc-DDK-tagged) - Mouse nephronophthisis 4 (juvenile) homolog (human) (Nphp4)
ACCESSION   NM_153424
VERSION     NM_153424.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5313..5404 
                     /label="Myc-DDK"  
     misc_feature    5434..6019 
                     /label="PolyA signal"  
     misc_feature    5491..5510 
                     /label="Restriction Enzyme"  
     misc_feature    6295..6967 
                     /label="ColE1"  
     misc_feature    7397..8191 
                     /label="Kan/Neo"  
     misc_feature    8226..8528 
                     /label="SV40 ori"  
     misc_feature    8614..9091 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5295..5301 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5295 
                     /label="Nphp4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGACTGG CACAGAGCCT TCACCCAGAA CACACTGGTC CCTCCCCACC 
     1081 CACAGAGAGC GAGGCAGCTT GGGAAGGAGT CCACGGCATT CCAGTGCATC CTGAAGTGGC 
     1141 TTGACGGGCC ACTCATTAAG CAGGGAATCC TAGACATGCT GTCGGAGCTT GAATGCCATC 
     1201 TGCGAGTGAC CCTCTTCGAT GTCACCTACA AACATTTCTT TGGGAGGACG TGGAAAACCA 
     1261 CAGTGAAGCC CACCAATCAA CCCTCTAAGC AGCCACCTAG GATCACCTTC AATGAGCCCT 
     1321 TGTACTTCCA CACGACCTTG AGCCACCCCA GCATCGTGGC TGTGGTAGAA GTGGTCACCG 
     1381 AAGGCAGGAA GAGGGACGGC ACCCTGCAGC TGCTGTCCTG TGGGTTTGGA ATTCTTCGCA 
     1441 TCTTTGGCAA TAAGCCAGAA TCTCCCACCT CTGCAGCCCA GGACAAACGG TTGAGGCTGT 
     1501 ATCATGGCAC CCCAAGAGCC CTCCTGCACC CACTTCTCCA GGATCCCATA GAACAGAACA 
     1561 AGTTCATGAG GCTGATGGAG AATTGTAGCC TGCAGTACAC CCTGAAGCCA CACCCACCCC 
     1621 TGGAGCCCGC CTTCCACCTG CTCCCTGAGA ACCTCCTGGT GTCTGGTTTC CAGCAGATCC 
     1681 CTGGCCTTCT CCCGCCTCAT GGGGACACGG GTGATGCCCT GCGGAAGCCT CGCTTCCAGA 
     1741 AGCCCACCAC ATGGCACTTA GATGACCTCT TCTTCACCCT GTACCCTTCC CTGGAGAAGT 
     1801 TTGAGGAGGA ACTTGTGCAG CTGCTCATCA GCGACCGGGA GGGGGTCGGC CTGCTGGACA 
     1861 GCGGCACCCT CGAGGTCCTG GAGCGGCGCC TGCACGTCTG CGTCCACAAC GGGCTGGGCT 
     1921 TCGTGCAGAG GCCACAGGTG GTTGTGCTTG TGCCTGAGAT GGACGTGGCC TTGACCCGCT 
     1981 CGGCCAGCTT CAGCAGGAAA ATCAGTGCCT CTTCCAAGAA CAGCTCTGGA AATCAGGCTC 
     2041 TAGTTTTGAG AAGCCACCTG CGACTCCCGG AGATGGTCAG TCACCCAGCC TTCGCCATCG 
     2101 TCTTCCAGCT GGAGTACGTG TTCAACAGCC CCTCTGGAGC GGATGGCGGT GCCAGCTCGC 
     2161 CCACCTCAAT ATCCAGCGTG GCGTGCATGC ACATGGTCCG ATGGGCTGTT TGGAACCCCG 
     2221 ATCTGGAAGT GGGCCCAGGA AAGGTGACGC TGCCTCTTCA GGGTGGGGTT CAGCAAAACC 
     2281 CCTCACGCTG TCTGGTCTAT AAGGTGCCTT CAGCTAGCAT GAGCTCCGAG GAGGTGAAGC 
     2341 AGGTGGAGTC CGGAACCATC CAGTTCCAGT TCTCACTGAG CTCAGACGGC CCCACAGAGC 
     2401 ATGCCAACGG CCCCAGGGTG GGGCGTCGGT CCTCCCGGAA GATGCCGGCA TCCCCTTCAG 
     2461 GCACCCCAGC ACCAGCTGCC CGGGATCTTG CTGCAACACA GGACTCTCCT GTGGGCCCAG 
     2521 GGCTCTCGCT CTCCCAGCTG ACGGCTTCCC CGCTGTCCCC GGCTCTGCAA AGCTCTTCCA 
     2581 AGCCCCCTCT TCAGCCCCCG GACAGCTCTC AGTCCCCAGA GGGTCCCCAG CTCCAGGCCG 
     2641 AATCTGTATT AGAGAGCAGG GTCTCTCACC TGGAAGCCGA CCTGAGCCAG CCGGCCTCGC 
     2701 TCCAGGGAAC GCCTGCTGTC GAGCATCTAC AGGAACTTCC CTTCACTCCT CTGCATGCCC 
     2761 CCATCGTCGT GGGAGCACAG ACCAGGAGCT CTAGAAGCCA GCTCTCCAGA GCTGCGATGG 
     2821 TGCTTCTGCA GTCTTCTGGC TTCCCCGAGA TCCTTGATGC CAGCCAGCAG CCCGTGGAGG 
     2881 CCGTCAACCC CATAGACCCC GTGAGGTTCA ACCCTCAGAA GGAGGAGTCG GACTGTCTTC 
     2941 GAGGGAATGA GATTGTGCTG CAGTTTCTTG CCTTCAGCAG AGCCGCCCAG GACTGCCCAG 
     3001 GAACGCCGTG GCCACAGACT GTGTATTTCA CCTTCCAGTT TTACCGTTTC CCACCCGAGA 
     3061 CCACGCCACG CCTGCAGCTG GTCAAGCTGG ATGGGACGGG CAAGAGCGGC TCTGGCTCCC 
     3121 TGTCCCACAT CCTCGTCCCC ATTAATAAAG ATGGCTCCTT TGATGCTGGC TCCCCCGGCC 
     3181 TCCAGCTGAG GTACATGGTG GATCCCGGGT TCCTGAAGCC AGGAGAGCAG CGCTGGTTCG 
     3241 CCCACTACCT GGCTGCTCAG ACGCTGCAGG TCGACGTCTG GGATGGAGAC TCCTTGCTCC 
     3301 TCATTGGGTC TGCTGGCGTC CAGATGAAGC ACCTCCTCCG CCAAGGGCGG CCAGCTGTTC 
     3361 AGGTCTCACA CGAACTGGAG GTCGTGGCCA CTGAGTACGA ACAGGAAATG ATGGCCGTGA 
     3421 GCGGCGATGT GGCTGGCTTC GGCAGCGTCA AGCCCATCGG AGTCCACACG GTAGTGAAGG 
     3481 GCCGCCTGCA CCTGACCTTG GCCAACGTGG GTCACGCTTG TGAGCCGAGA GCAAGAGGTT 
     3541 CCAACTTGCT GCCGCCATCC AGGTCTCGGG TCATCTCAAA TGATGGAGCC AGCTTCTTCT 
     3601 CTGGGGGAAG CCTCCTCATA CCCGGAGGCC CCAAGCGAAA GCGTGTGGTA CAGGCCCAGA 
     3661 GGCTGGCTGA TGTGGACAGC GAGCTGGCCG CCATGCTGCT GACACACACT CGAGCTGGCC 
     3721 AGGGGCCCCA GGCTGCAGGC CAGGAGGCAG ACGCTGTGCA CAAGCGGAAG CTGGAGAGGA 
     3781 TGCGGCTGGT GCGCCTGCAG GAGGCTGGAG GGGACTCTGA CAGTCGCAGG ATCAGCTTGC 
     3841 TGGCCCAACA CAGTGTCCGT GCACAGCACT CACGGGACTT GCAGGTCATT GATGCCTACC 
     3901 GGGAACGTAC CAAGGCCGAG AGCATTGCCG GAGTGCTAAG CCAGGCCATC ACCACCCACC 
     3961 ACACGCTGTA CGCTACACTG GGCACCGCTG AGTTCTTCGA GTTTGCACTT AAAAACCCAC 
     4021 ACAACACTCA GCACACGGTG GCCATCGAGA TCGACAGCCC TGAGCTCAGT ATCATCCTGG 
     4081 ACAGCCAGGA GTGGAGGTAC TTCAAGGAGG CCACCGGCCT GCACACGCCC CTGGAGGAGG 
     4141 ATATGTTCCA CCTGCGTGGG AGCCTGGCAC CCCAGCTCTA CCTGCGACCC CGGGAGACTG 
     4201 CCCACATCCC CCTTAAGTTC CAGAGCTTCT CCGTGGGCCC TCTTGCTCCG ACACAGGCCC 
     4261 CTGCTGAGGT GATCACAGAG AAGGACGCAG AGTCTGGACC CCTTTGGAAG TGCAGCGCCA 
     4321 TGCCTACGAA ACACGCCAAG GTCCTTTTCC GGGTGGAGAC TGGCCAGCTC ATTGCTGTGC 
     4381 TCTGCCTGAC CGTGGAGCCC CAGCCCCACG TGGTGGACCA GGTTTTCCGC TTCTACCACC 
     4441 CAGAGCTCAC GTTCCTGAAG AAGGCCATCC GCCTGCCTCC CTGGCACACG CTTCCAGGTG 
     4501 CTCCTGTGGG GATGCCTGGC GAGGATCCCC CAGTGCATGT TCGATGCAGT GATCCAAATG 
     4561 TCATCTGTGA GGCCCAGAAC GTGGGCCCCG GTGAGCCGAG AGATGTGTTC CTTAAGGTGG 
     4621 CCAGTGGTCC AAGCCCGGAG ATCAAAGACT TCTTTGTCGT CATCTATGCG GATCGCTGGC 
     4681 TGGCGGTGCC CGTGCAGACG TGGCAGGTCT GCCTCCACTC CCTGCAGCGC GTGGATGTTT 
     4741 CCTGTGTCGC AGGACAGCTG ACCCGTCTGT CCCTCGTCCT TCGGGGGACA CAGACCGTTA 
     4801 GGAAAGTGAG AGCTTTCACC TCCCACCCCC AGGAGCTGAA GACAGACCCT GCCGGTGTCT 
     4861 TTGTGTTGCC ACCTCATGGG GTACAGGACC TGCATGTGGG TGTGAGGCCA AGGAGGGCTG 
     4921 GCAGCCGCTT TGTCCATCTC AACCTAGTGG ACATAGACTA CCATCAGCTG GTTGCGTCAT 
     4981 GGCTTGTGTG CCTCTCCTGC CGCCAGCCAC TCATCTCCAA GGCCTTCGAG ATCACCATGG 
     5041 CAGCAGGGGA TGAGAAGGGC ACCAACAAGC GCATCACCTA CACCAACCCC TATCCTTCTC 
     5101 GGAGGACCTA CCGCCTGCAC AGTGACCGTC CCGAGCTGTT GCGCTTCAAG GAGGACTCCT 
     5161 TCCAGGTGGC GGGAGGAGAG ACCTACACCA TCGGCCTGCG GTTCCTGCCA AGTGGCAGCG 
     5221 CCGGGCAGGA GGAGATCCTG ATCTACATCA ATGACCACGA GGACAAGAAT GAGGAGACAT 
     5281 TCTGTGTGAA GGTCCTCTAC CAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5341 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5401 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5461 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5521 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5581 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5641 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5701 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5761 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5821 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5881 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5941 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6001 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6061 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6121 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6181 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6241 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6301 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6361 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6421 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6481 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6541 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6601 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6661 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6721 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6781 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6841 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6901 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6961 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7021 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7081 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7141 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7201 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7261 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7321 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7381 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7441 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7501 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7561 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7621 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7681 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7741 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7801 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7861 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7921 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7981 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8041 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8101 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8161 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8221 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8281 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8341 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8401 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8461 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8521 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8581 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8641 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8701 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8761 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8821 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8881 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8941 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9001 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9061 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9121 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//