LOCUS       MR216506                9213 bp    DNA     circular  SYN 
DEFINITION  Arap1 (Myc-DDK-tagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (Arap1), transcript variant 3
ACCESSION   NM_001040111
VERSION     NM_001040111.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5368..5459 
                     /label="Myc-DDK"  
     misc_feature    5489..6074 
                     /label="PolyA signal"  
     misc_feature    5546..5565 
                     /label="Restriction Enzyme"  
     misc_feature    6350..7022 
                     /label="ColE1"  
     misc_feature    7452..8246 
                     /label="Kan/Neo"  
     misc_feature    8281..8583 
                     /label="SV40 ori"  
     misc_feature    8669..9146 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5343..5350 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5343 
                     /label="Arap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAGGGT TACGATGCCG CACTGTCTGT AGCTGAGTGG CTGCGGGCAC 
     1081 TTCACCTGGA GCAGTACACG GCACTCTTTG AGCAGCACGG ACTGGTGTGG GCCACCGAGT 
     1141 GCCAAGGCCT CAGCGATGCT GGCCTGCTAG ACATGGGCAT GCACCTCCCC GGCCACCGCC 
     1201 GCCGCATCCT GGCTGGTCTT CACCGCGCCC ATGCACCCCC AGTCCCTCTG CCTCGCCCCG 
     1261 CCCCACGGCC CGTGCCGATG AAGCGACACA TCTTCCGTTC ACCACCTGTG CCCGTCACTC 
     1321 CACCAGAGCC GCCGCCCACA GCTGGAGAGG ATGAGGGTCT GCCTGCCGCT CCACCTATCC 
     1381 CACCCCGCAG GAGCTGCCTC CCACCTGCCT GCTTCACTCC GACATCCACA GCCGCCCCAG 
     1441 ATCCTGTGCT GCCCCCACTG CCTGCCAAGA GACACTTGGT AGAACCCAGC GTTCCTCCTG 
     1501 TCCCTCCTCG AACCGGACCC CCCTACCCAC AGGCCAGCCT TCTTGCTAAG GAGGAGTTAC 
     1561 TGCTGCCATC AGTGTCACCC CGGTCCCAAC CAGAGCCTGC AGAGACTCCG TCCACCCTCC 
     1621 TGCCTGCCTT TCCCCAGGGG CCCCTGCAGC CTCCGTCTCC TCCTCCCTGT CCTCCTGTGA 
     1681 TCCCTCCCAA GCCTCCTCGC CTGCTCCCTG AGTTTGACGA CTCTGACTAT GATGATGTCC 
     1741 CGGAGGAGGG GCCAGGGGCC CCAGCCAGTG TGATGACCAA GGAGGAGCCC CTGCCGAGCC 
     1801 GAGTCCCACG GGCTGTGCGT GTGGCTAGTC TGCTGAGCGA GGGGGAGGAG CTGTCTGGGG 
     1861 ATGATTCGGA AGATGACGAT GACCATGCCT ATGAGGGTAT CCCCAATGGT GGGTGGCCAA 
     1921 CGAGTGGCCT GAATCCACCC TTACGCAGCC TGATCCCTGA TCTCCCACTG CACCCCATGG 
     1981 ATGAGTTGCC TGGGGGTCCC ACCCCCATCA CACCTGTCAT CAAGGCTGGC TGGCTGGACA 
     2041 AGAACCCACC ACAGGGGTCT TATATCTATC AGAAGCGATG GGTGAGACTG GACGCTGATT 
     2101 ACCTGCGATA CTTTGACAGT AACAAGGACG CCTACTCTAA GCGCTTTGTT CCTGTGGCCT 
     2161 GCATCTGCCG AGTAGCTCCT ATTGGAGACC AGAAGTTTGA AGTGATCACA AATAATCGGA 
     2221 CCTTCGCCTT CCGGGCAGAG AGTGATGTGG AGCGGAACGA GTGGATGCAG GCCCTGCAGC 
     2281 AGGCAGTAGT TGAGCATCGA GCCCGTTTTC GGCTTTCTAG TGCTTCTGTG TTGGGAGTCC 
     2341 GAGGCTCCGA GCAGCCTGAC CGTGCTGGCA GCCTGGAGCT ACGAGGCTTC AAGAATAAGC 
     2401 TTTACGTGGC TGTGACTGGG GACAAAGTAC AGCTCTATAA GAATCTGGAG GAATTCCATC 
     2461 TGGGCATTGG TATCACCTTT ATCGACATGA ATGTGGGCAA TGTCAAGGAA GTGGATCGGC 
     2521 GCAGCTTTGA CCTCACTACC CCCTACCGCA TCTTCAGCTT CTCGGCTGAT TCAGAGTTAG 
     2581 AGAAGGAACA GTGGCTGGAG GCCATGCAGG GAGCCATCGC AGAGGCCCTA TCTACCTCAG 
     2641 AGGTGGCTGA GCGCATCTGG GCCGCAGCCC CCAACAGGTT CTGTGCCGAC TGTGGGGCCG 
     2701 CCCAGCCTGA CTGGGCCTCC ATCAACCTCT GCGTTGTCAT CTGCAAGCGC TGTGCAGGGG 
     2761 AGCACCGCGG CCTGGGTGCA GGAGTGTCCA AGGTGCGGAG TTTGAAGATG GACAGGAAGG 
     2821 TGTGGACAGA AGCACTCATC CAGCTCTTCT TACATCTGGG CAATGGCCCC GGGAACCACT 
     2881 TCTGGGCTGC TAATGTGCCT CCTAGTGAGG CCCTGGAGCC CAGCAGCAGC CCTGGTGCCC 
     2941 GGCGGTATCA CCTGGAGGCC AAGTACAGAG AGGGCAAGTA CCGCCGCTAC CATCCGCTCT 
     3001 TTGGCAACCA AGAGGAACTG GACAAGGCCC TGTGTGCTGC AGTTACTACC ACTGACCTGG 
     3061 CTGAGACCCA GGCACTCCTG GGCTGTGGGG CTGGGGTCAG CTGCTTCTCA GGGGACCCAG 
     3121 CAGCTCCCAC ACCCCTGGCT CTTGCTGAGC AGGCCGGACA GACTCTGCAG ATGGAGTTTC 
     3181 TACGGAATAA TCAGAGTACA GAGGTCCCTC GGTTGGACTC AGTGAAGCCC TTGGAAAAGC 
     3241 ACTACTCCGT TACCCTGCCA ACTGTGAGCC ACAGCGGCTT CCTGTACAAA ACTGCTTCTG 
     3301 CCGGCAAACC TCTGCAGGAC CGCCGTGCCC GGGAAGAGTT CAGCCGGCGC TGGTGTGTCC 
     3361 TGAGTGATGG GGTCCTGAGC TACTATGAAA ACGAACGGGC AGTGACACCC AATGGGGAGA 
     3421 TTCGGGCCAG CGAGATAGTA TGCCTAGCAG TTTCCCCTCT GGACACCCAT GGCTTTGAGC 
     3481 ACACCTTTGA GGTGTACACA GAGGGAGAAC GGCTGTACCT GTTTGGGCTG GAGAATGCAG 
     3541 AGCTGGCTCA TGAGTGGGTC AAGTGCATTG CCAAGGCGTT CGTGCCTCCC CTGGCTGAGG 
     3601 ACCTGCTAGC CCGGGACTTT GAACGTCTTG GGCGCCTACC CTGTAAAGCT GGCCTGAGCC 
     3661 TTCAGCAGGC TCAGGAAGGC TGGTTTGCCT TGACTGGCTC TGAGCTCCGG GCTGTCTTCC 
     3721 CAGAGGGGCC CTGGGAAGAG CCGCTGCAGC TCCGGAAACT GCAAGAGCTT TCTATCCAAG 
     3781 GAGACAGCGA GAACCAAGTG CTGGTGCTGG TGGAGCGGAG GAGGACACTG TACATCCAGG 
     3841 GTGAGCGGCG GCTGGACTTC ATGGCTTGGC TAGGGGTCAT CCAGAAAGCA GCAGCCAGCT 
     3901 TGGGAGACAC ACTATCAGAA CAACAGCTTG GGGACTCGGA CATCCCAGTG ATTGTGTACC 
     3961 GCTGTGTGGA CTACATCACG CAGTGTGGCT TGACCTCAGA GGGTATCTAT CGAAAGTGTG 
     4021 GTCAGACCTC CAAGACTCAG AGACTGCTAG ACAGCCTCCG GCAGGACGCA CGCTCTGTGC 
     4081 ACCTAAAGGA GGGAGAACAG CACGTGGACG ACGTCTCCTC TGCACTCAAA CGCTTCCTCA 
     4141 GGGACCTGCC CGATGGGCTC TTCACGCGTG CGCAGCGCCT GGCCTGGCTG GAGGCCTCTG 
     4201 AGATCGAGGA TGAGGAAGAA AAGATCTCCA GGTATCGAGA GCTCTTGGTG CATCTGCCCC 
     4261 CTGTCAACCG GGCCACTGTG AAGGCCCTTA TCAGCCATCT GTACTGTGTA CAGTGCTTCT 
     4321 CAGACACAAA CCAAATGAAC ACACACAACC TGGCTATCGT GTTTGGGCCT ACACTCTTCC 
     4381 AGACAGACGG GCAGGACTAC AAGGCCGGCA AGGTGGTGGA AGACCTCATC AACCACTACG 
     4441 TGGTGGTGTT CAGTGTGGAC GAGGAGGAGC TGAGGAAGCA GAGGGAGGAA GTCACGGCCA 
     4501 TCGTGAAGAT GCGAGTGGCT GGCACTGCCA GTGGGACCCA GCATGCTGGC GACTTCATCT 
     4561 GCACAGTCTA CCTGGAGGAG AAGAAGGTGG AGACTGAACA GCATGTTAAG ATCCCAGCAT 
     4621 CCATGACTGC AGAGGAGCTT ACTCTGGAGA TTCTGGACCG CCGCAATGTG AGCATCAGGG 
     4681 AGAAGGACTA CTGGACTTGC TTTGAGGTCA ACGAGAAGGA GGAGGCAGAG CGCCCGCTGC 
     4741 ACTTTGCAGA GAAGGTGCTG CCCATTGTCC ATGGGCTGGG CATAGACAGC CATCTGGTGG 
     4801 TGAAGAAGTA CCAGTCCATG GAGGCCATGC TGTTGTACTT GGCCAGCCGT GTGGGTGACA 
     4861 CCAAGCATGG TATGATGAAG TTCCGTGAAG ACCGCAGCCT CCTGGGTCTG GGGCTACCTT 
     4921 CGGGTGGCTT CCACGATCGC TACTTCATTC TCAACAGCAG CTGCCTACGG CTCTACAAGG 
     4981 AGGTCCGGAG TCACCGGCCT GAGAAGGAGT GGCCTGTCAA GAGCCTCAAA GTCTACCTGG 
     5041 GTGTAAAGAA GAAACTGCGG CCACCTACTT GCTGGGGCTT CACGGTGGTG CACGAGACAG 
     5101 AAAAGCACGA GAAGCAGCAG TGGTACCTCT GCTGTGACAC GCAGATGGAA CTTCGAGAGT 
     5161 GGTTTGCCAC CTTCCTCTCT GTGCAGCACG ATGGCCTGGT GTGGCCCTCC GAGCCATCTC 
     5221 GAGTGTCCCG GGCAGTGCCT GAGGTCCGGA TGGGCAGTGT ATCGCTGATC CCTCTACGAG 
     5281 GCAGTGAAAA TGAAATGCGC CGGAGTGTGG CAGCCTTCAC TGCTGACCCC CTTTCCCTTC 
     5341 TCCGCCATGT CAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5401 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5461 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5521 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5581 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5641 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5701 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5761 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5821 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5881 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5941 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6001 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6061 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6121 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6181 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6241 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6301 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6361 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6421 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6481 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6541 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6601 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6661 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6721 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6781 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6841 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6901 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6961 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7021 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7081 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7141 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7201 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7261 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7321 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7381 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7441 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7501 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7561 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7621 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7681 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7741 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7801 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7861 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7921 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7981 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8041 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8101 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8161 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8221 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8281 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8341 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8401 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8461 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8521 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8581 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8641 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8701 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8761 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8821 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8881 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8941 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9001 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9061 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9121 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9181 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//