LOCUS       MR216492                8724 bp    DNA     circular  SYN 
DEFINITION  Pitpnm2 (Myc-DDK-tagged) - Mouse phosphatidylinositol transfer protein, membrane-associated 2 (Pitpnm2)
ACCESSION   NM_011256
VERSION     NM_011256.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4881..4972 
                     /label="Myc-DDK"  
     misc_feature    5002..5587 
                     /label="PolyA signal"  
     misc_feature    5059..5078 
                     /label="Restriction Enzyme"  
     misc_feature    5863..6535 
                     /label="ColE1"  
     misc_feature    6965..7759 
                     /label="Kan/Neo"  
     misc_feature    7794..8096 
                     /label="SV40 ori"  
     misc_feature    8182..8659 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4863..4869 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4863 
                     /label="Pitpnm2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCATAAAG GAATACCGGA TTCCCCTGCC AATGACCGTG GATGAATACC 
     1081 GCATTGCCCA GCTGTACATG ATACAGAAGA AGAGCCGGAA TGAGACCCAC GGGCAAGGCA 
     1141 GCGGGGTAGA GATCCTGGAG AACCGGCCCT ACACAGACGG CCCTGGCGGC TCCGGGCAAT 
     1201 ACACCCACAA GGTGTATCAT GTGGGCATGC ACATTCCTGG CTGGTTTCGC TCCATCTTGC 
     1261 CCAAAGCAGC CTTGCGGGTG GTGGAGGAGT CCTGGAATGC CTACCCCTAT ACCCGAACAA 
     1321 GGTTCACCTG CCCCTTTGTG GAGAAGTTCT CCATTGACAT TGAAACCTTT TATAAGACAG 
     1381 ACACTGGGGA AAACAATAAT GTGTTCAACC TGTCTCCTGT GGAAAAGAGC CAGCTGATAA 
     1441 CAGACATCAT CGACATCGTC AAGGATCCTG TGCCCCCCAG TGAGTATAAG ACAGAAGAAG 
     1501 ACCCCAAGCT GTTCCAGTCA GTCAAGACTT GCCGGGGACC TCTTTCTGAA AACTGGATTC 
     1561 AGGAGTACAA GAAGCGGCTC CTCCCCATCA TGTGCGCCTA CAAGCTCTGC AAGGTGGAGT 
     1621 TCCGATACTG GGGCATGCAG TCCAAGATTG AAAGGTTCAT CCATGACACA GGTCTGCGGC 
     1681 GGGTGATGGT GAGGGCCCAC CGGCAGGCCT GGTGCTGGCA GGACGAGTGG TACGGACTGA 
     1741 CCATGGAGAA AATCCGAGAG CTGGAGAGGG AGGTGCAGCT CATGCTGTCC CGCAAAATGG 
     1801 CCCAGTTTTC GGAGGAAGGC CCCTCAGAAC TAAGCAAGGA CAGTGCCACC AAGGACCAGG 
     1861 CATCTGGAAC AACCTCTGAT CCTGGCAGCA AAAACGGGGA GCCTCTGGGG CGGGGCCTGA 
     1921 AGAAGCAGTG GTCCACCTCC TCCAAGTCCT CAAGGTCCTC CAAGCGGGGA GCCAGCCCCT 
     1981 CCCGACATAG CATCTCAGAG TGGAGGATGC AGAGTATCGC CAGGGACTCC GACGAAGGCT 
     2041 CGGAAGAGGA GTTCTTCGAT GCACATGAGA ACCTGTACTG CACAGAGGAA AAACAAGCCA 
     2101 AGGACATGAC CAAGTGGAAC TCCAACGACC TCATGGACAA AATGGAGAGT CCGGAGCCTG 
     2161 AGGAATCACA AGATGAAATC TACCAGCAGA GTGGCTCTGA GTTCAGGGTG GCCTCCAGCG 
     2221 TGGAGCAGCT GAACATCATC GAGGATGAGG TGAGCCAGCC ACTAGCCGCG CCACCCTCCA 
     2281 AGATTCACGT GCTGCTGCTG GTGCTGCATG GAGGCACCAT CCTGGACACT GGAGCGGGGG 
     2341 ACCCCAGCTC CAAGCAGGGG GACACCAATA CTATCACCAA CGTGTTCGAC ACCGTCATGC 
     2401 GCGTGCACTA CCCCAGTGCC CTAGGCCACC TAGCCATCCG CCTGGTGCCC TGCCCACCCA 
     2461 TCTGTGCTGA TGCCTTCGCC CTCGTCTCCA ACCTCAGTCC CTATGGCCAT GATGAGGGCT 
     2521 GTCTGTCTAG CAGCCAGGAC CACATCCCCC TAGCTGCCCT GCCCCTGCTG GCCACTTCCT 
     2581 CACCCCAGTA CCAGGAGGCA GTTGCTACAG TGATTCAGCG GGCCAACCTG GCCTACGGAG 
     2641 ACTTCATCAA GTCCCAGGAA GGTGTGACCT TCAATGGTCA GGTCTGCCTG ATTGGAGACT 
     2701 GTGTTGGGGG CATCCTGGCA TTTGATGCCT TATGCTACAG TGGCCAGCCA GTGTCTGAGA 
     2761 GTCAAAGCAG TAGCCGCCGA GGCAGTGTGG TCAGCATGCA GGACGCTGAC CTGCTGTCCC 
     2821 CGGGCACCCT GGCGAATGCA GCACACTGCT CCGGTGGCAG TGGCGGTGGC GGCAGCGGAG 
     2881 GCTCCAGCCT GGAGAGCAGT CGGCACCTGA GCCGCAGCAA CATTGACATC CCCCGAAGCA 
     2941 ACGGCACTGA GGACTCCAGA AGGCAGCTGC CCCGCAAAAG GAGTGACTCG TCCACCTATG 
     3001 AGCTGGACAC CATCCAGCAG CACCAGGCCT TCCTATCCAG CCTCCACGCC AGCGTGCTGA 
     3061 GGAATGAGCC CAGCTCCCGC CGCTCAAGCA GTTCCACGAT GCTGGACGGT GCCGGGGCCC 
     3121 TGGGCAAGTT TGACTTCGAG ATTGCCGACC TCTTCCTCTT CGGGTGCCCG CTGGGGCTAG 
     3181 TCCTAGCCTT GAGGAAAACA GTCATCCCTT CCCTGGACGT TTTCCAGCTG CGTCCAGCGT 
     3241 GTCAGCAAGT GTACAACCTC TTCCACCCCG CGGACCCCTC AGCCTCCCGC CTGGAGCCGC 
     3301 TGCTGGAGCG GCGTTTCCAC AGCCTGCCGC CTTTCAGCAT CCCTCGGTAC CAGCGCTACC 
     3361 CGCTGGGGGA CGGCTGCTCC ACACTGCTGG CGGATGTGCT TCAGACCCAC AACACAGTCT 
     3421 TCCAAGAGCA TGCAGCCCCC TCGTCACCTG GCACAGCCCC TGCTGGCCGT GGCTTCCGCA 
     3481 GAGCCAGTGA GATCAGTATC GCCAGTCAGG TGTCAGGCAT GGCCGAGAGC TACACAGCGT 
     3541 CCAGCATCGC CCAGATTGCT GCCAAGTGGT GGGGACAGAA GCGGATTGAC TATGCCCTGT 
     3601 ATTGCCCGGA CGCCCTCACG GCCTTCCCCA CGGTGGCCTT GCCCCACCTC TTCCACGCCA 
     3661 GCTACTGGGA GTCAACCGAC GTGGTCTCCT TTCTGCTGAG ACAGGTCATG CGGCACGACA 
     3721 GCTCGAGCAT CCTAGAGCTG GACGGCAAGG AGGTGTCCGT GTTCACGCCT TCACAGCCCA 
     3781 GGGAAAGGTG GCAGCGCAAG AGGACCCACG TGAAGCTGCG GAACGTGGCA GCCAACCACC 
     3841 GGATCAATGA CGCAGTTGCC AATGAGGATG GCCCGCAGGT TGTGACGGGC CGGTTCATGT 
     3901 ATGGGCCCTT AGACATGGTC ACCCTGACTG GGGAAAAGGT GGATGTGCAC ATCATGACGC 
     3961 AACCACCTTC AGGTGAGTGG CTGCACCTAG ACACTCTAGT GACCAACAGC AGTGGGCGCG 
     4021 TCTCCTACAC CATTCCAGAA ACACACCGCC TGGGGGTGGG CGTCTACCCC ATCAAGATGG 
     4081 TGGTCAGGGG AGACCACACA TTCGCCGACA GCTACATCAC TGTGCTCCCC AGGGGCACAG 
     4141 AGTTTGTGGT CTTCAGTATA GACGGCTCCT TTGCCGCCAG TGTGTCCATC ATGGGCAGCG 
     4201 ACCCCAAGGT GCGCGCCGGG GCTGTGGACG TGGTGCGACA CTGGCAGGAC CTGGGCTACC 
     4261 TCATCATCTA TGTGACCGGC CGGCCCGACA TGCAAAAGCA GCGGGTGGTG GCATGGCTGG 
     4321 CTCAGCACAA TTTCCCTCAT GGTGTGGTGT CCTTCTGTGA TGGCCTGGTG CACGACCCAC 
     4381 TGCGCCACAA GGCTAACTTT CTGAAGCTGC TCATCTCTGA GCTTCACCTG CGCGCACATG 
     4441 CGGCCTACGG CTCCACGAAG GATGTCGCAG TCTATAACTC CATCAGCCTC TCTCCCATGC 
     4501 ACATCTACAT TGTGGGCCGC CCCACCAAGA AGCTGCAGCA GCAGTGCCAG TTCATCACGG 
     4561 ACGGCTATGC GGCCCACCTG GCCCAGCTCA AGTACAATCA CCGGGCTCGC CCAGCCCGGA 
     4621 ACACGGCCAC GCGCATGGCT TTGCGCAAGG GCAGCTTCGG CCTACCTGGC CAGAGTGACT 
     4681 TCCTTCGCTC TCGGAATCAC CTGCTCCGCA CCATCTCGGC CCAGCCCAGT GGGCCCAGCC 
     4741 ATCGGCACGA TCGGACACAG ACCCAGATGG ACAGCGAGCA GCGGGGCCAG CGCAGCATGA 
     4801 GCGTGGCAGC CAGCTGCTGG GGCCGAGCCA TGGCAGGCCG GCTTGAACCA GGGGCAGCCA 
     4861 CGGGACCCAA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4921 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4981 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5041 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5101 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5161 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5221 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5281 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5341 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5401 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5461 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5521 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5581 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5641 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5701 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5761 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5821 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5881 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5941 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6001 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6061 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6121 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6181 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6241 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6301 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6361 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6421 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6481 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6541 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6601 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6661 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6721 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6781 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6841 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6901 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6961 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7021 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7081 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7141 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7201 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7261 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7321 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7381 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7441 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7501 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7561 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7621 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7681 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7741 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7801 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7861 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7921 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8161 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8221 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8281 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8341 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8401 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8461 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8521 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8581 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8641 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8701 TAACAAAAAT TTAACGCGAA TTTT
//