LOCUS       MR216472                8175 bp    DNA     circular  SYN 
DEFINITION  Magi2 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 2 (Magi2), transcript variant 3
ACCESSION   NM_001170745
VERSION     NM_001170745.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4332..4423 
                     /label="Myc-DDK"  
     misc_feature    4453..5038 
                     /label="PolyA signal"  
     misc_feature    4510..4529 
                     /label="Restriction Enzyme"  
     misc_feature    5314..5986 
                     /label="ColE1"  
     misc_feature    6416..7210 
                     /label="Kan/Neo"  
     misc_feature    7245..7547 
                     /label="SV40 ori"  
     misc_feature    7633..8110 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4314..4320 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4314 
                     /label="Magi2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATTGGAG AAAAGTGGTG CTCTCCTAGA AAGCGGGACC TATGAAGACA 
     1081 ACTACTACGG TACCCCGAAG CCTCCAGCTG AACCAGCACC ATTATTAAAT GTAACAGACC 
     1141 AGATACTTCC GGGAGCTACT CCAAGTGCTG AGGGGAAGCG GAAAAGAAAT AAGTCAGTGA 
     1201 CCAACATGGA GAAAGCAAGT ATAGAGCCTC CAGAGGAGGA AGAAGAAGAA AGGCCTGTAG 
     1261 TCAATGGAAA CGGCGTGGTC ATAACCCCAG AATCCAGTGA ACATGAAGAC AAAAGTGCAG 
     1321 GTGCCTCAGG GGAGACACCC TCCCAGCCTT ACCCTGCACC CGTGTACAGC CAGCCCGAAG 
     1381 AGCTCAAGGA CCAGATGGAC GATACAAAGC CAACAAAGCC TGAGGAGAAC GAGGACTCTG 
     1441 ATCCATTGCC TGATAACTGG GAAATGGCCT ACACAGAGAA GGGGGAAGTC TACTTCATTG 
     1501 ACCATAACAC AAAGACAACA TCATGGCTGG ATCCGCGACT TGCGAAAAAG GCTAAACCTC 
     1561 CAGAAGAGTG CAAAGAAAAT GAGCTTCCAT ATGGCTGGGA AAAAATCGAT GATCCTATAT 
     1621 ATGGCACTTA CTATGTTGAC CACATAAATA GAAGAACACA GTTTGAAAAC CCTGTCCTGG 
     1681 AAGCAAAAAG GAAGCTACAG CAACATAACA TGCCCCACAC AGAACTTGGA GCAAAGCCCC 
     1741 TGCAGGCCCC AGGTTTCCGA GAAAAGCCAC TCTTCACCCG GGATGCATCC CAGTTGAAGG 
     1801 GAACGTTCCT CAGCACCACC CTCAAAAAGA GCAACATGGG CTTTGGGTTT ACCATCATTG 
     1861 GTGGAGACGA GCCGGATGAG TTTCTACAGG TGAAAAGTGT GATCCCGGAT GGGCCTGCCG 
     1921 CACAGGATGG GAAAATGGAG ACAGGTGATG TCATTGTCTA TATTAATGAA GTTTGTGTCC 
     1981 TTGGACACAC TCATGCAGAT GTTGTCAAAC TTTTCCAGTC TGTTCCTATT GGTCAGAGTG 
     2041 TCAACTTGGT GTTGTGTCGT GGCTACCCTT TGCCCTTTGA CCCTGAAGAT CCTGCTAACA 
     2101 GCATGGTGCC ACCCCTTGCA ATAATGGAGA GGCCACCTCC GGTGATGGTC AATGGAAGAC 
     2161 ATAACTATGA AACATACTTG GAATACATTT CTCGGACCTC ACAGTCGGTC CCAGATATTA 
     2221 CAGACCGGCC ACCTCATTCT TTGCACTCCA TGCCAGCTGA CGGCCAGCTA GATGGCACGT 
     2281 ATCCACCACC CGTCCATGAC GACAATGTGT CTATGGCTTC GTCTGGAGCC ACTCAAGCTG 
     2341 AACTTATGAC CTTAACCATT GTGAAAGGTG CCCAGGGATT TGGCTTTACT ATTGCCGACA 
     2401 GTCCCACGGG ACAGCGGGTG AAACAAATCC TTGACATTCA GGGATGCCCT GGGCTGTGTG 
     2461 AAGGAGACCT CATTGTTGAG ATCAACCAAC AGAATGTACA GAACCTGAGC CATACAGAAG 
     2521 TAGTGGATAT ACTTAAGGAC TGCCCCGTTG GAAGTGAGAC TTCTTTAATC ATCCATCGAG 
     2581 GAGGTTTCTT TTCTCCATGG AAAACTCCAA AGCCTATGAT GGACCGATGG GAGAACCAAG 
     2641 GCAGTCCACA AACAAGTTTA TCTGCTCCGG CCGTCCCACA GAACCTGCCC TTCCCACCTG 
     2701 CCCTTCACAG GAGCTCCTTT CCTGATTCAA CAGAGGCCTT TGACCCACGG AAGCCTGACC 
     2761 CATATGAGCT CTACGAGAAA TCGAGAGCCA TTTATGAAAG TAGGCGTCCA GATTATAAGG 
     2821 AACTGGATGT TCACCTTCGG AGGATGGAGT CTGGATTTGG CTTTAGAATC CTTGGGGGAG 
     2881 ATGAACCTGG ACAGCCTATT TTGATCGGAG CCGTCATTGC CATGGGCTCA GCTGACAGAG 
     2941 ACGGCCGTCT ACACCCAGGA GATGAGCTTG TCTATGTCGA TGGGATCCCA GTGGCTGGCA 
     3001 AGACCCACCG CTATGTCATC GACCTCATGC ACCACGCGGC CCGCAATGGG CAGGTTAACC 
     3061 TCACTGTGAG AAGAAAGGTG CTATGTGGAG GGGAGCCCTG CCCAGAGAAT GGGAGGAGTC 
     3121 CAGGCTCTGT ATCAACTCAC CACAGCTCTC CGCGCAGTGA CTATGCCACC TACTCCAACA 
     3181 GCAACCACGC CGCCCCCAGC AGCAATGCCT CACCTCCTGA AGGCTTTGCC TCACACAGCT 
     3241 TGCAGACCAG TGATGTGGTC ATTCACCGCA AAGAAAACGA AGGGTTTGGC TTCGTCATCA 
     3301 TCAGCTCTCT GAACAGGCCT GAGTCTGGAG CCACCATAAC TGTGCCCCAT AAAATTGGAC 
     3361 GAATCATTGA TGGGAGCCCT GCAGATCGCT GTGCCAAACT CAAAGTGGGC GACCGTATCT 
     3421 TAGCAGTCAA CGGCCAGTCT ATCATCAACA TGCCTCACGC TGACATTGTG AAGCTCATCA 
     3481 AGGACGCCGG TCTCAGTGTC ACCCTTCGCA TCATTCCTCA GGAGGAGCTC AACAGCCCAA 
     3541 CATCAGCACC CAGTTCAGAG AAACAGAGCC CCATGGCCCA GCAGCACAGC CCTCTGGCCC 
     3601 AGCAGAGTCC TCTGGCCCAG CCAAGCCCCG CCACCCCCAA CAGCCCAGTC GCACAGCCAG 
     3661 CTCCTCCCCA ACCTCTCCAG CTGCAAGGAC ACGAAAATAG TTACAGGTCA GAAGTTAAAG 
     3721 CGAGGCAAGA TGTGAAGCCA GACATCCGGC AGCCTCCCTT CACAGACTAC AGGCAGCCCC 
     3781 CGCTGGACTA CAGGCAGCCC CCGGGAGGAG ACTACTCACA GCCCCCACCC TTGGACTACA 
     3841 GGCAGCACTC TCCAGACACC AGGCAGTACC CTCTGTCAGA CTACAGGCAG CCACAGGATT 
     3901 TTGATTATTT CACTGTGGAC ATGGAGAAAG GAGCCAAAGG ATTTGGATTC AGCATTCGTG 
     3961 GAGGAAGGGA ATACAAGATG GATCTGTATG TGTTGAGATT GGCAGAGGAT GGGCCAGCCA 
     4021 TAAGGAACGG CAGGATGAGG GTAGGAGATC AGATCATTGA AATAAATGGG GAAAGCACAC 
     4081 GAGACATGAC CCACGCCAGA GCAATAGAAC TCATCAAGTC TGGAGGAAGA AGAGTGCGGC 
     4141 TGCTGCTGAA GAGAGGCACG GGGCAGGTCC CGGAGTATGG AATGGTACCT TCCAGCCTCT 
     4201 CCATGTGCAT GAAAAGTGAC AAGCATGGGT CCCCATATTT CTACTTACTG GGCCACCCTA 
     4261 AAGACACGAC GAACCCCACG CCTGGAGTGC TGCCGCTGCC GCCGCCCCAG GCCTGCCGGA 
     4321 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4381 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4441 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4501 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4561 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4621 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4681 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4741 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4801 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4861 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4921 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4981 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5041 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5101 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5161 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5221 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5281 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5341 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5401 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5461 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5521 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5581 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5641 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5701 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5761 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5821 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5881 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5941 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6001 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6061 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6121 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6181 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6241 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6301 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6361 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6421 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6481 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6541 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6601 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6661 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6721 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6781 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6841 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6901 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6961 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7021 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7081 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7141 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7201 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7261 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7321 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7381 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7441 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7621 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7681 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7741 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7801 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7861 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7921 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7981 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8041 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8101 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8161 TTTAACGCGA ATTTT
//