LOCUS       MR216435                9381 bp    DNA     circular  SYN 
DEFINITION  Sycp2 (Myc-DDK-tagged) - Mouse synaptonemal complex protein 2 (Sycp2)
ACCESSION   NM_177191
VERSION     NM_177191.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5538..5629 
                     /label="Myc-DDK"  
     misc_feature    5659..6244 
                     /label="PolyA signal"  
     misc_feature    5716..5735 
                     /label="Restriction Enzyme"  
     misc_feature    6520..7192 
                     /label="ColE1"  
     misc_feature    7622..8416 
                     /label="Kan/Neo"  
     misc_feature    8451..8753 
                     /label="SV40 ori"  
     misc_feature    8839..9316 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5520..5526 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5520 
                     /label="Sycp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGTGAGA CCAGACCTCC AACAGTTAGA AAAGTGTATT GATGATGCTT 
     1081 TGAGAAAAAA TGACTTCAAA CCTTTGTTGG CACTTTTACA AATTGATATT TGTGAAGATG 
     1141 TGAAGATTAA ATGCAGCAAA CAATTCCTCC GCAAGTTGGA TGACTTAATA TGCAGGGAAC 
     1201 TTAATAAAAA GGATATCCAA ACTGTTTCAA GCATCTTGAT ATCTATTGGA AGATGTAGCA 
     1261 AGAATATCTT TATATTGGGA CAAGCTGGAC TTCAAACCAT GATAAAACAA GGATTAGTCC 
     1321 AAAAGATGGT TTCCTGGTTT GAAAATTCCA AGGAGATTAT TCTAAATCAA CAACAATCAA 
     1381 AAGATGAAGC TGTTATGAAT ATGATAGAAG ACTTATTTGA TCTTTTGATG GTCATATATG 
     1441 ACATCAGTGA TGAAGGTAAA AACCAAGTAT TGGAAAGTTT CATACCTCAA ATCTGTGCCC 
     1501 TGGTTATTGA TTCAAGAGTG AATTTTTGCA TTCAGCAGGA GGCTTTAAAA AAAATGAATT 
     1561 TGATGCTTGA CAGAATACCT CAAGATGCCA ACAAAATACT TTCTAATCAA GAAATGTTAA 
     1621 CTCTCATGAG TAATATGGGA GAAAGGATTT TAGATGTAGG AGATTATGAA TTACAGGTAG 
     1681 GCATTGTGGA AGCTTTGTGT AGAATGACCA CAGAAAAACG GAGACAAGAA CTGGCATATG 
     1741 AATGGTTTTC AATGGACTTT ATTGCTAATG CATTTAAGGA AATTAAAGAC TGTGAATTTG 
     1801 AAACAGATTG CAGAATATTT CTCAACTTGG TAAATGGCAT TCTTGGTGAT AAAAGAAGGG 
     1861 TCTATACATT TCCTTGCTTG TCAGCATTTC TTGGTAAATA TGAGCTGCAG ATACCATCAG 
     1921 ATGAAAAACT TGAGGAATTC TGGATCGATT TTAATCTTGG GAGCCACACT CTGTCATTCT 
     1981 ACATTGCTGG AGATGAAGAA GATCACCAAT GGGAAGCTGT CACTGTACCT GAAGAAAAAG 
     2041 TTCAGATGTA CAACATTGAA GTGAGAGAAT CAAAGAAGCT ACTGACTCTA ACTTTGAAAA 
     2101 ATATAGTAAA AATTAGTAAA AAAGAAGGAA AAGAGTTACT TTTTTATTTT GATGAATCAT 
     2161 TAGAAATCAC CAACGTGACT AAAAAAGTTT TTGGTGGAAA TAAGTATAAG GAATTTACCA 
     2221 GAAAACAAGG TATTTCAGTT GCCAAAACAT CTATTCATGT ACTTTTTGAT GCAAGTGGAT 
     2281 CACAGATTCT AGTACCAGAA AGTCAACCAT CTCCAGTCAA AGAAAACCTC ATTCATCTAA 
     2341 AAGAGAAATC CGACATCCAA AAGAAACTTG TAAACCCTCT AGAACTAGGC AATAGCAGCA 
     2401 GCCAGGATGA GATCACTACA CCTAGCAGAA AGAAAATGTC TGAAGCATCA ATGATTGTTC 
     2461 CTGATACAGA CAGATACACT GTGCGAAGCC CAATACTTTT AATCAACACA TCAACACCGC 
     2521 GAAGAAGTAG GGAACCACTG CAAGCAATAA ATTCTGTGGA GAAAGCTGTT TCTAAAACAT 
     2581 CAGAAAGTGG AATGGATTAT GCTGCGTCAC CCAAATCTAG ACAATCAGAT GGAAGAAAAA 
     2641 GATGGAATAA TAGAGCCAAC CATAACAAAA CTACTGCTGT CATACAAAAC AAACAATACG 
     2701 AGGATAATGA ATCCCCAGAC CAAAATTTCA ATGAAATTGA GGACACTCTC TCTAATGTAT 
     2761 CTTCTGCAGT GGGAAAAGTA GACAAGCCTG TATTGCCTGG TGTTTTAGAC ATCTCAAAAA 
     2821 ATACAACACA CTCCAGATGG GCATGTTGGA CACCTGTAAC AACTATCAAA CTCTGCAATA 
     2881 ACCAGAGAAG CCGTGCTTTA CCTGGAGACA CTTGTACCCA AGATACTGGT GTCAACAAAA 
     2941 AATGCACTAA ACAAAAATCA GTATCAGATG ATGATTCTGA AGAAACACAA AAGGGAAAAT 
     3001 ATAGTAAAGA TGTAATCAAG TGTAACAAGT CAGATGAAGC AGAATTTTGT GAAAGAAACA 
     3061 TTCAAGAACA AAATCATCCT AAATATTCAC AAAAGAAAAA TACTGCAAAT GCAAAGAAGA 
     3121 GTGATTGGCA TATTGAATCT GAAACTACTT ATAAATCTGT ACTCCTAAAT AAGACAACTG 
     3181 AAGAATCTCT CATCTATAAG AAGACATGTG TATTGTCAAA AGATGTGAAT ACTACTATCT 
     3241 GTGATAAAAG CCCTTCTAGA AAAAGCAAGA GGAATCATAC AAAATCAAGA AAGGAACTGA 
     3301 TGTCTGAACT TACATCATGT GAGCTAGAAG AAATACCAGT GAGAGAAAAT TCAAAAGGGA 
     3361 AAAGATTTAC TGGTGCATCA GAATCCTTGA TAAACCAAAT TAGTAGGAGA TATAACCCAA 
     3421 GTGATAGCAT GATGTCAACA AGAAAACTGA AGGAGCCTCA GGATGGCAGT GGATTTTCAA 
     3481 AAAAACCTGA TCTGCAGTTC AATAAGGTTC AGAGAAAGAG TTACAGGAAA CTGAAGGCAA 
     3541 CTGTTGTCAA TGTTACTTCT GAATGTCCAC TGGATGATGT ATATAATTTC AGCTTGAATG 
     3601 GTGCCGATGA ACCTGTTATA AAACTTGGAA TCCAAGAATT TCAAGCTACA ACTAGAGAAG 
     3661 CCAGTATGGA TAATTCATTA AAATTGGTAA AGAATCATGA TGAACATGAC CCTTTTCTCA 
     3721 AAACAAAAGA TAAAAGAATG TTAAGTTATG AGAAGAAAAC TCTCTTAAGT GACACTGAAA 
     3781 CCGAATGTGG ATGTGATGAC AGCAAGACTG ACATTAGCTG GCTAAAAGAA CCAAAAACAA 
     3841 AAAGACTAAT GGATTATAGT AGAAATAAAA ACACAACAAA ATATAAAAGT AGAAAATCAA 
     3901 GATCATCCAT GGAAAAAGGA CAACCAAGAC CCACAATGGT ACTCAATAAA AACAGTATGA 
     3961 AAAATGATTA TGAAGTAGTT GTAGATGGGA GAACCAGACT TCCACGAAGA GCAACAAAAA 
     4021 CAAAAAAAAA TTATAAAGAT CTTTCAACTT CAGAATCAGA ATCAGAGAGT GAAAAAGAAT 
     4081 GTTCATATTT GTTTAAAGAT AAACTGCCAA CAAAGGAGGA GACTATCCAT TCCAGAGCCC 
     4141 AAACAAAGAA ACTGCCCGAG AAACAACAGA AAGTCTTCAA TTCAGAAGCG CTGAAAGGAC 
     4201 AGCCATCAGA AGAACAGAAA AACTCCTCTC GGCTGAGAGA AGGGAGAGAA GACAGTCTGT 
     4261 GCCTGTCTTC TGCGTCTGTG TCCAGGAGCT CGTCCTCTGT GGAAGTGATG AGATGTACAG 
     4321 AGAAAATAAC AGAAAGGGAT TTTACTCAGG ATTATGACTA TATCACAAAA TCTCTTTCAC 
     4381 CTTATCCAAA AGCTCCATCA CCTGAATTCT TAAATGGAAA CAATAGCGTT GTAGGTCGGG 
     4441 GACAATCACC CAGAATTAGT GAGACCAGTG CAATGTGTGT AAGAAAGAGT TACTCACCTG 
     4501 CTTCAGGACC GCCCTTTTCG CCAAGACACA CTCCGACCAA GAATAATTCT GTTGTGAATA 
     4561 TGAAAAAAGC AAATTCAGTG ATAAATAATC AGAGAACCCA ACATTGTAAC AGCTATTCAG 
     4621 ATGTAAGCAG TAATAGCTCA GAGAAACTTT ATATGGAACC TGAATCTCCA GAGAGCTGTG 
     4681 ACAACCATAT GCAAAACAAG AGAGAGGGAA ATCATGCAGC ATCTCCATTA TCATTGTCTA 
     4741 GTGAAAAAAT AGAGAAAATG TGGTTTGACA TGCCCAGTGA AAATACTCAT GTATCAGGTC 
     4801 CCAGTCAACG TGGTAGCAAA AGGCGGATGT ACCTAGAAGA TGATGAGCTA AGTAATTCCA 
     4861 ATGAAGCAGA AGTAGAAGAG GCAGAAGAAA GGGAACATTT GCTTTCCAAA AAACGATGTC 
     4921 AATGGGAAAA TTCTGACCAG CACACCTTCA AAACTTCATT ATCGACACCA GATTTTTCTG 
     4981 TTCCTAAGGA CTGGCAACAA GAGTTACAAG GTGCTGGAAT GTTTTATGAT AACATCAGCT 
     5041 CAGACTATAA AAGGAAAACT GATAGCCAAC ATAAAATCAT GGATGATTTT ACTACAAAGA 
     5101 CATTGAAATT GACTCAACAA CATCTGATGG CAATGACCTC TCAAGCTCAG GGACGCAGGG 
     5161 ATGAAAATGT TGAGAAATTC CAAGTCACTC TCCTAGATGA GCTGGAAAAA GTTGAAAAAG 
     5221 ACTCACAGAC TTTGCGAGAC TTGGAGAAAG AGCTTGTGGA CATCGAGGAA AAGTTAGTTC 
     5281 AGAAGATGAG GGCATATCAC CGATGTGAGC GAGAGAGGTT TCGTGTTTTG AAAACTTCAC 
     5341 TGGATAAAAG TTTTCTTGTC TATAATTCTG TTTATGAAGA GTCTGTTTTT ACATCTGAGA 
     5401 TGTGTTTGAT GAAAGCAAAC ATGAAAATGC TACAAGACAA GCTACTTAAG GAGATGCATG 
     5461 AAGAGGAAGT TCTCAACATA CGCAGAGGAC TACAGTCATT ATTCAAGGCT CATGAAGGAA 
     5521 ATGATGCAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5581 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5641 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5701 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5761 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5821 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5881 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5941 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     6001 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     6061 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     6121 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6181 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6241 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6301 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6361 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6421 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6481 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6541 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6601 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6661 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6721 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6781 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6841 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6901 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6961 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     7021 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     7081 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     7141 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7201 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7261 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7321 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7381 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7441 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7501 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7561 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7621 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7681 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7741 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7801 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7861 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7921 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7981 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     8041 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     8101 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     8161 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8221 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8281 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8341 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8401 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8461 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8521 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8581 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8641 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8701 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8761 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8821 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8881 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8941 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     9001 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     9061 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     9121 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9181 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9241 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9301 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9361 CAAAAATTTA ACGCGAATTT T
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