LOCUS       MR216423                7212 bp    DNA     circular  SYN 
DEFINITION  Chpf (Myc-DDK-tagged) - Mouse chondroitin polymerizing factor (Chpf), transcript variant 1
ACCESSION   NM_001001566
VERSION     NM_001001566.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3367..3458 
                     /label="Myc-DDK"  
     misc_feature    3488..4073 
                     /label="PolyA signal"  
     misc_feature    3545..3564 
                     /label="Restriction Enzyme"  
     misc_feature    4349..5021 
                     /label="ColE1"  
     misc_feature    5451..6245 
                     /label="Kan/Neo"  
     misc_feature    6280..6582 
                     /label="SV40 ori"  
     misc_feature    6668..7145 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3342..3349 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3342 
                     /label="Chpf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGCGTCG CTGCTGCTGT CCGTGCTGCG GCCCGCGGGG CCCGTGGCCG 
     1081 TGGGCATCTC TCTGGGCTTC ACCCTGAGCC TGCTCAGCGT CACCTGGGTG GAGGAGCCTT 
     1141 GCGGACCCGG GCCGCCCCAA CCCGGAGACT CTGAGCTGCC GCCGCGCGGC AACACCAACG 
     1201 CGGCGCGCCG GCCCAACTCG GTGCAGCCCG GATCCGAGCG CGAGAGGCCC GGGGCCGGCG 
     1261 CAGGCACCGG TGAGAGCTGG GAGCCTCGTG TCTTGCCCTA CCATCCCGCG CAGCCAGGCC 
     1321 AGGCCACCAA GAAGGCCGTC AGAACTCGGT ATATCAGCAC GGAGCTGGGC ATCAGGCAGA 
     1381 AGCTTCTGGT GGCAGTGCTG ACCTCACAAG CCACGTTGCC TACACTGGGT GTGGCTGTAA 
     1441 ACCGAACTCT GGGACACCGA CTGGAGCATG TAGTGTTCCT GACCGGTGCG AGGGGCCGCC 
     1501 GGACACCTTC AGGCATGGCG GTGGTGGCAC TGGGCGAAGA GAGGCCCATC GGACACCTGC 
     1561 ACCTGGCGCT GCGCCACCTG CTGGAGCAAC ACGGCGATGA CTTTGACTGG TTTTTCCTAG 
     1621 TGCCTGATGC CACCTATACT GAAGCGCATG GACTGGACCG CCTAGCTGGC CACCTCAGCC 
     1681 TTGCTTCAGC AACCCATCTC TATCTTGGCC GGCCGCAGGA CTTCATCGGT GGAGATACTA 
     1741 CCCCAGGCCG CTACTGCCAC GGGGGCTTTG GAGTCTTGCT CTCTCGCACA CTGCTACAGC 
     1801 AACTGCGCCC CCACCTGGAA AGCTGCCGCA ACGACATCGT CAGTGCTCGC CCGGATGAGT 
     1861 GGTTGGGCCG CTGCATCCTT GATGCCACAG GCGTGGGCTG TACTGGTGAC CACGAGGGAA 
     1921 TGCACTACAA CTACCTGGAA CTGAGCCCCG GGGAGCCTGT GCAGGAGGGG GACCCTCGTT 
     1981 TCCGCAGCGC CTTGACAGCC CATCCCGTGC GTGACCCTGT GCACATGTAC CAGCTGCACA 
     2041 AAGCTTTTGC CCGCGCTGAG CTGGACCGCA CGTACCAGGA GATTCAAGAA TTGCAGTGGG 
     2101 AGATCCAGAA TACCAGCCGA CTGGCTGCTG ATGGGGAGAG AGCCTCTGCC TGGCCAGTGG 
     2161 GCATCCCAGC ACCGTCTCGC CCTGCCTCAC GCTTTGAGGT TCTGCGCTGG GACTACTTCA 
     2221 CAGAACAATA CGCGTTCTCC TGCGCCGATG GCTCTCCCCG CTGCCCGTTG CGTGGGGCCG 
     2281 ACCAGGCTGA TGTGGCTGAC GTCCTGGGGA CAGCCTTAGA GGAGCTCAAC CGCCGTTACC 
     2341 AGCCAGCGCT GCAGCTCCAG AAGCAACAGC TGGTGAACGG CTACCGGCGT TTTGATCCAG 
     2401 CCCGAGGCAT GGAGTACACA CTAGACCTGC AGCTGGAAGC GCTGACACCC CAGGGTGGCC 
     2461 GCTGGCCCCT CACCCGCAGG GTGCAGCTCC TTCGGCCCTT GAGCCGAGTG GAGATCTTGC 
     2521 CTGTACCCTA TGTCACCGAG GCTTCTCGGC TCACTGTGCT ACTGCCGCTG GCTGCAGCGG 
     2581 AACGAGACCT GGCTTCTGGC TTCTTAGAAG CCTTTGCCAC TGCAGCCCTG GAACCTGGTG 
     2641 ATGCAGCAGC CTTGACCCTG CTGCTGCTGT ATGAGCCACG CCAGGCCCAG CGGGCAGCCC 
     2701 ACTCAGACGT CTTCGCACCT GTCAAGGCCC ACGTGGCAGA GCTAGAGCGG CGTTTCCCTG 
     2761 GTGCCCGGGT GCCCTGGCTC AGTGTGCAGA CAGCAGCGCC CTCTCCACTG CGTCTCATGG 
     2821 ATCTGCTGTC CAAGAAGCAC CCACTAGACA CTCTGTTCCT GCTGGCCGGG CCAGACACGG 
     2881 TACTCACACC TGATTTCCTG AACCGCTGCC GCATGCATGC CATCTCTGGC TGGCAGGCCT 
     2941 TCTTCCCCAT GCACTTCCAG GCCTTCCACC CTGCTGTGGC TCCACCTCAG GGCCCTGGGC 
     3001 CACCAGAGCT GGGCCGTGAC ACCGGTCACT TTGATCGCCA GGCTGCCAGT GAGGCATGCT 
     3061 TCTACAACTC CGACTATGTG GCGGCCCGTG GCCGGCTGGT GGCGGCCTCG GAGCAGGAGG 
     3121 AGGAGCTGCT GGAGAGCCTG GATGTGTACG AGTTGTTTCT GCGCTTCTCC AACTTGCACG 
     3181 TGCTGAGAGC AGTAGAGCCA GCCTTGCTGC AGCGCTACCG GGCCCAGCCG TGCAGTGCAC 
     3241 GGCTCAGTGA AGACCTTTAC CACCGCTGTC GCCAGAGCGT ACTTGAGGGC CTTGGCTCCC 
     3301 GCACCCAGCT TGCCATGCTG CTCTTTGAGC AGGAACAGGG GAACAGCACC AGCGGACCGA 
     3361 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3421 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3481 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3541 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3601 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3661 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3721 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3781 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3841 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3901 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3961 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4021 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4081 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4141 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4201 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4261 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4321 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4381 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4441 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4501 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4561 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4621 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4681 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4741 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4801 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4861 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4921 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4981 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5041 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5101 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5161 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5221 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5281 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5341 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5401 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5461 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5521 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5581 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5641 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5701 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5761 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5821 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5881 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5941 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6001 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6061 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6121 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6181 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6241 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6301 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6361 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6421 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6481 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6541 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6601 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6661 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6721 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6781 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6841 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6901 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6961 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7021 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7081 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7141 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7201 AACGCGAATT TT
//