LOCUS       MR216408                8481 bp    DNA     circular  SYN 
DEFINITION  Atp13a1 (Myc-DDK-tagged) - Mouse ATPase type 13A1 (Atp13a1)
ACCESSION   NM_133224
VERSION     NM_133224.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="Atp13a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGGTG GGCAACGCGG TTCCTTGCGG AGCCCGGCCT GGCGGGGCCC 
     1081 GGGACAACGG GTCTCCTCAG CCTGGGTCGC GATTGCGGCC GGGCCTAGCT GCCGGGCCAG 
     1141 CACTCATAGC GAACGGCGAC GAACTGGTGG CCGCCGTGTG GCCGTACCGG CGGCTGGCGC 
     1201 TTCTGCGGCG CCTCACCGTG CTGCCGTTTG CCGGGCTGCT GTACCCAGCC TGGCTGGGCG 
     1261 CCGCCGCCAG CGGTTGCTGG GGATGGGGCA GCAGCTGGAC CCAGATACCC GAGGCCGCGC 
     1321 TGCTGGCGCT CGCCACCATC TGCCTCGCGC ACGCACTCAC TGTCCTGTCG GGGCATTGGT 
     1381 CTGTGCACGC GCACTGCGCG CTCACCTGCA CCCCGGAGTA TGATCCCAAC AAAGTAACCT 
     1441 TTGTGAAGGT GGTGCCAACC CCCAACAATG GCTCCACTGA GCTTGTGGCC CTGCACCGTG 
     1501 ACAAGGGTGA AGATGGGCTG GAGGTGCTGT CATTTGAATT CCAGAAGATC AAATATTCCT 
     1561 ATGATGCCCT GGAGAAAAAG CAGTTCCTTC CGGTGGCCTT CCCAGTGGGG AATGCCTTCT 
     1621 CTTACTATCA AAGCAACAGA GGCTTCCAGG AAGACTCAGA GATCCGAGCT GCAGAGAAGA 
     1681 AGTTTGGAAG CAACAAGGCT GAGATGGTAG TGCCCGACTT CTCTGAGCTT TTCAAGGAGA 
     1741 GAGCCACAGC CCCTTTCTTC GTGTTTCAGG TATTCTGCGT TGGGCTCTGG TGCCTGGACG 
     1801 AGTATTGGTA CTACAGTGTC TTCACTCTGT CCATGCTGGT GGCTTTCGAA GCTTCCCTAG 
     1861 TGCAGCAGCA GATGAGGAAC ATGTCAGAGA TCCGAAAGAT GGGCAACAAG CCCCATATGA 
     1921 TACAGGTCTA CCGCAGCCGA AAGTGGAGGC CTGTCGCCAG TGATGACATT GTTCCTGGGG 
     1981 ACATCGTGTC TATCGGCCGC TCACCCCAGG AGAACTTGGT GCCCTGTGAT GTACTGCTGC 
     2041 TACGGGGCCG CTGCATTGTA GACGAGGCCA TGCTCACTGG GGAATCTGTG CCACAAATGA 
     2101 AGGAGCCCAT TGAAGACCTG AGCCCAGACC GTGTCCTAGA CCTGCAGGCC GATGCTCGGC 
     2161 TGCATGTCAT CTTTGGGGGC ACCAAGGTGG TACAGCACAT TCCCCCGCAG AAGGCCACCT 
     2221 CGGGCCTGAA GCCGGTCGAC AATGGATGTG TGGCCTTTGT CCTGAGAACT GGGTTCAACA 
     2281 CATCCCAGGG CAGACTACTG CGTACAATTC TCTTTGGGGT AAAGAGGGTC ACTGCAAACA 
     2341 ACTTGGAGAC CTTTATCTTC ATCCTCTTCC TCCTGGTCTT CGCCATTGCT GCAGCTGCGT 
     2401 ATGTGTGGGT TGAAGGGACC AAGGACCCCA GCCGGAACCG TTACAAGCTC TTTCTGGAGT 
     2461 GCACCCTCAT CCTCACCTCT GTTGTACCCC CTGAACTGCC CATTGAACTG TCTCTGGCTG 
     2521 TCAACACCTC CCTCATCGCC TTGGCCAAGC TCTACATGTA CTGCACAGAG CCTTTCCGGA 
     2581 TCCCCTTCGC TGGCAAGGTT GAAGTGTGTT GCTTTGACAA GACGGGTACC TTGACCAGTG 
     2641 ACAGCCTGGT GGTGCGAGGC GTGGCAGGGC TGAGAGATGG GAAAGAGGTG ACCCCAGTGT 
     2701 CCAGCATTCC CATAGAAACA CACCGGGCCC TGGCCTCCTG CCACTCGCTC ATGCAGCTGG 
     2761 ATGACGGCAC CCTAGTGGGT GACCCTCTGG AAAAGGCCAT GCTGACAGCT GTGGACTGGA 
     2821 CACTGACCAA AGATGAGAAA GTATTCCCCC GAAGTATTAA AACTCAGGGG CTGAAAATTC 
     2881 ACCAGCGCTT TCATTTTGCC AGTGCCCTAA AGCGAATGTC TGTGCTCGCC TCCTACGAGA 
     2941 AGCTTGGCTC CACTGACCTC TGCTACATCG CGGCTGTGAA GGGGGCCCCC GAAACCCTGC 
     3001 ACTCTATGTT TTCTCAGTGC CCACCTGACT ACCACCACAT CCACACTGAG ATCTCTCGGG 
     3061 AAGGAGCCCG TGTCCTGGCT CTGGGATACA AGGAGCTAGG ACATCTCACA CATCAGCAGG 
     3121 CCCGGGAGAT CAAGCGGGAG GCACTGGAGT GCAGCCTCAA GTTTGTGGGC TTCATCGTGG 
     3181 TCTCCTGTCC CCTCAAAGCT GACTCCAAGG CTGTGATCCG AGAGATCCAG AATGCATCCC 
     3241 ACAGGGTGGT CATGATCACA GGAGACAACC CCCTCACTGC ATGCCATGTA GCTCAGGAGC 
     3301 TGCACTTCAT TGACAAAGCC CACACGCTCA TCTTGCATCC TCCCTCGGAG AAAGGCCAGC 
     3361 CATGTGAGTG GCGCTCCATT GATAGCAGCA TCGTTCTGCC TCTCACCCTG GGCTCCCCCA 
     3421 AGGCACTGGC CCTGGAGCAC GCGCTATGCC TCACAGGCGA TGGCTTGGCT CACCTGCAGG 
     3481 CTGTGGACCC CCAGCAGCTG CTCTGCCTCA TCCCTCATGT GCAGGTGTTT GCCCGTGTAG 
     3541 CCCCTAAGCA GAAGGAATTT GTCATCACTA GCCTGAAGGA GCTGGGTTAT GTGACCCTTA 
     3601 TGTGTGGAGA TGGCACCAAC GATGTGGGCG CACTGAAGCA CGCTGATGTG GGTGTAGCAC 
     3661 TCCTGGCCAA TGCCCCTGAG AGGGTTGTAG AACGGCGGCG ACGGCCAAGG GACAGCCCTG 
     3721 TCCTGAGCAA CAGTGGCCCC AGAGTCTCCA GGTCCACCAA ACAGAAGTCG GCACTCCTCT 
     3781 CTCCTGAGGA GCCACCAGCT TCCCACAGGG ACCGCCTGAG CCAGGTGCTG CGGGACCTGG 
     3841 AGGAGGAGAG CACCCCCATC GTGAAGCTGG GGGATGCCAG CATCGCAGCA CCCTTCACCT 
     3901 CCAAGCTGTC CTCCATCCAG TGCATCTGCC ACGTGATCAA GCAGGGCCGG TGCACATTGG 
     3961 TGACGACGCT GCAGATGTTC AAGATCCTGG CCCTCAATGC CCTCATCCTG GCATACAGCC 
     4021 AAAGTGTCCT CTACCTGGAG GGTGTCAAGT TCAGTGACTT CCAGGCCACA CTGCAGGGGC 
     4081 TGCTGCTGGC CGGCTGCTTC CTCTTCATCT CCCGCTCCAA GCCTCTCAAG ACTCTTTCCC 
     4141 GAGAGCGGCC CCTACCCAAC ATCTTCAACC TGTACACCAT CCTTACGGTG ATGCTGCAAT 
     4201 TCTCTGTCCA CTTCCTGAGC CTGGTCTACC TGTACCGTGA GGCCCAGGCA CGCAGCCCTG 
     4261 AGAAGCAGGA GCAGTTTGTA GACCTGTACA AGGAGTTTGA GCCGAGCCTG GTCAACAGCA 
     4321 CTGTGTACAT CATGGCCATG GCCATGCAGA TGGCCACCTT CGCCATCAAC TACAAGGGCC 
     4381 CACCCTTCAT GGAGAGCCTG CCTGAGAACA AGCCCCTGGT GTGGAGTCTG GCAGTGTCAC 
     4441 TGCTGGCCAT CATTGGCTTG CTCCTTGGTT CCTCACCTGA CTTCAACAGC CAGTTTGGCC 
     4501 TTGTGGACAT CCCTGTAGAG TTCAAGCTGG TCATCGGCCA GGTCCTGGCC CTGGACTTCT 
     4561 GCCTGGCACT CCTGGCTGAC CGTGTCCTAC AGTTCTTTTT GGGGACTCCG AAGCTGAGAG 
     4621 TGCCTTCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//