LOCUS       MR216392                7353 bp    DNA     circular  SYN 
DEFINITION  Ttc16 (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 16 (Ttc16)
ACCESSION   NM_177384
VERSION     NM_177384.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3510..3601 
                     /label="Myc-DDK"  
     misc_feature    3631..4216 
                     /label="PolyA signal"  
     misc_feature    3688..3707 
                     /label="Restriction Enzyme"  
     misc_feature    4492..5164 
                     /label="ColE1"  
     misc_feature    5594..6388 
                     /label="Kan/Neo"  
     misc_feature    6423..6725 
                     /label="SV40 ori"  
     misc_feature    6811..7288 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3492..3498 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3492 
                     /label="Ttc16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAGACAGA AGCAAGACTC AGAGAACTCC ATCTGCCTCT AGCTCTAGAA 
     1081 CGGGGTTATT AAAGGAGCCT CACAGTGCCC GTTCCCAGAA AACTCCAAAG CTAAAGGTTC 
     1141 CTGAAGCCCC AGAGAAGATC CTGCGACGCA TCTTTGGGAC CAGTCATGTG TTCTATGTTA 
     1201 TGACAGATCC TACAAAACGA AGGATGATAG GATCAACAGT GCCTGCCAAA GTCAGAGAGT 
     1261 ACTATAACCA AGGCCATCAG TGCCTCCTAC AAGAGGACTG GGAGATGTCT GTGCTGTTCT 
     1321 TCTCCCGTGC CCTCCACCTG GACCCCAAAC TGGTAGACTT CTATGTATTC CGTGCTGAGG 
     1381 CCTTCATCCA ACTCTGTGAC TTCTCCTCTG CCCTCCAGAA CCTCCGCAGG GCCTACTCCT 
     1441 ATGACCCAGG GAATAACAAG TACCTGAACC GGCTGGCCTT TGTGCTCTAC CTACAGGGTC 
     1501 AGTGCCTGTA TGAGCTGTGT GACTTCCAGG AAGCCCTCTG TGTCTTCCTG CAAGCCTCCG 
     1561 ACCTCCAGCC CCAAAACGCT TCCTTCAGTT ACCGATGCAT GGCCTGCCTG CTGGCCCTCA 
     1621 AGCGGTATCA CGACTGCCTC GCCCTCATCA CTAGGGAGGT GAAGCAGGGC AGGGCCAGCG 
     1681 CTGATGTCTA CATCCTCCGG GCTCGGCTCT ACAACTTCTT CCAGAAGGCC AAGCTCTGCT 
     1741 ATCAGGACCT TCGAAGCGCC TTGCTCCTAG ATCCCTTGCA CGCACAGGCC AAAGGGCTAC 
     1801 TCCAGAAGAT GGTGGACCAG GCCAAGCAGT CTCTCCAAGA TGCCAGCACC CTGGCCGTGC 
     1861 AGGGCAAGGT GCACCGTGCA CTGAAGTGCA TCAACTGTGC CATTGAGAAC AATCCCCTGG 
     1921 ATCCCAACTT CTTCTTCTTC CGGGGCACCT TGCGTAGACG GCTCCAGCAA TTTGACCATG 
     1981 CTGTGGAAGA CTTCCTGAAG GCAATGGACA TGGTGACTGA CACCCAGGAC AACCTAGTGA 
     2041 AGCAGGCGCA GCGTCAGCTG CTGCTGACTT ACAATGACTT TGCTGTGCAC TGCTACAACC 
     2101 ATGGTGCCTA TCAGGAGGGC GTGCTGCTGC TGAACAAGGC TATCAGAGAC GAGCAGAATG 
     2161 AGAAGGGCTT GTACATCAAC CGTGGGGATT GCTTCTTCCA GCTGGGCAAC CTGGCCTTCG 
     2221 CGGAGGCTGA CTACAAGCAA GCGCTGGCCC TGAGCCCGCT GGATGAAGGA GCCAACTTGC 
     2281 GCATGGGTGT GCTGCAGGAG AAGCTGGGCT TCTGCCAGCA GAAACACAGG CAGTTCCAGA 
     2341 CAGCAGAGGA GCACTTCTCG GAGGCCATAA GGCACAGCCC TCAGAAACCA CAGTACTACC 
     2401 TGCACCGGGC CAAGTGCCGA CAGTTCCTGC AGAACACTCT GGGGGCCCGC CTGGATGTCG 
     2461 CTACTGTACT GCTTCTCAAT CCCGAGTATC CCAAGATGGC TGCAGTGATG AACACCCTCT 
     2521 TTCCTAGCAT GACTGTGGAG AATGTGCTTA AGTCCCAAGT GGCCGAACTG GCCAAGCTCC 
     2581 AGCTAAGTCG GATGATAGAA AATGGGCCGA AGAACATCTA CCCTCAGAGC ACTGTGGTGC 
     2641 AGCGGTTATT GGAACGCAGG AAAGCCCAAG TCCTGGTGAA GCTCTGGAAG CAGGAACGTC 
     2701 TTGGGACCCC TGAAGAGGAA GTGACTCTTT ACCAGGCTCC GCAGCTGGCA GAGGAAAAAA 
     2761 AGGTGAAAAC CGCTCGCAGA AGGACATCCC TGACAGACAG CTATGCGGAC CAGACCTCCT 
     2821 CAGGCTCCGT CTTTAGCATT GTGAGCATCA GCACGTCGGG GCCAGAGATG TCAACCAGTC 
     2881 AGGAGTATAA GAGCTCTTCC CACACGGCTA TAGAATCCTC TGAGTCCACA CTGCTAAAGC 
     2941 CTCAGCTCTC AGTACCCAGG AAGAGCCAGG AACTGACCTG GAGCCCCAAG GTCGTCCAGG 
     3001 CAGTCACAGA GAACCTAATC CAGAACGCCA CTGAGGTCAC ACCTGCCTAT GGCCAAAGGG 
     3061 ACTCCAAAAA GGCCACCCAA GTCCCAAAGC CCAAGAAGAC AGAGGATCCC AAGGATCCTA 
     3121 GCCAGAGCAC CAGCACGACA GAAGCCCCTG AGGGCCCAAG GCCTAGCAAG TCCAGGTCTA 
     3181 CCCTGAGCGT AAAGGAGAGA ATCAGAAGAG CTAAGGCTGT TCGCGCCCAG GGCTGGAAAC 
     3241 TCAAGGCCCA GCGCAGCAGC CAGAAAGTAA CAAAGACTCC CAGCCTGACC CACAGCACGA 
     3301 CCCATAGTGA CATTGGTGAG AGTGCCAATG ATACCCCGGG CCAGACTCCA TGGCCCAGCA 
     3361 AGGCTGCTGA TAGCTTAAGC TTCAGTGAGA TCTCGTCAAC AGATCTCAGC AGTAGTGAAA 
     3421 GCTTCTTGGA ACTGACTAAC CTTTTAACTC AGGAAGTCCA ACAGATCCCA GGAGACAGAG 
     3481 AGAAGCTCAC CTCTGACGAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3541 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3601 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3661 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3721 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3781 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3841 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3901 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3961 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4021 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4081 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4141 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4201 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4261 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4321 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4381 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4441 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4501 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4561 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4621 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4681 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4741 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4801 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4861 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4921 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4981 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5041 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5101 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5161 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5221 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5281 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5341 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5401 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5461 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5521 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5581 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5641 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5701 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5761 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5821 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5881 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5941 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6001 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6061 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6121 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6181 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6241 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6301 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6361 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6421 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6481 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6541 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6601 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6661 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6721 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6781 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6841 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6901 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6961 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7021 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7081 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7141 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7201 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7261 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7321 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//