LOCUS       MR216282                8490 bp    DNA     circular  SYN 
DEFINITION  Nek1 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1)
ACCESSION   NM_175089
VERSION     NM_175089.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4647..4738 
                     /label="Myc-DDK"  
     misc_feature    4768..5353 
                     /label="PolyA signal"  
     misc_feature    4825..4844 
                     /label="Restriction Enzyme"  
     misc_feature    5629..6301 
                     /label="ColE1"  
     misc_feature    6731..7525 
                     /label="Kan/Neo"  
     misc_feature    7560..7862 
                     /label="SV40 ori"  
     misc_feature    7948..8425 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4629..4635 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4629 
                     /label="Nek1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGTAT GTGAGACTGC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG 
     1081 CTGTTCTTGT TAAATCGACA GAGGATGGCA GACATTATGT CATCAAGGAA ATTAACATCT 
     1141 CAAGAATGTC TGATAAAGAA AGGCAAGAAT CAAGGAGAGA AGTTGCTGTA TTGGCAAACA 
     1201 TGAAGCATCC AAATATTGTC CAATATAAAG AATCATTTGA AGAAAATGGC TCTCTCTACA 
     1261 TAGTAATGGA TTACTGTGAA GGAGGTGATT TGTTTAAACG AATAAATGCT CAGAAAGGCG 
     1321 CTCTGTTTCA AGAAGACCAG ATTTTGGACT GGTTTGTGCA GATATGTTTG GCTCTGAAGC 
     1381 ATGTACATGA TAGAAAAATT CTTCACCGAG ACATAAAGTC ACAGAACATA TTTCTAACCA 
     1441 AAGATGGGAC AGTGCAGCTT GGAGATTTTG GAATTGCTCG AGTTCTTAAT AGTACTGTAG 
     1501 AGCTGGCTCG AACTTGCATA GGCACTCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA 
     1561 AGCCTTATAA CAATAAAAGT GACATTTGGG CTTTGGGCTG TGTCCTTTAT GAGTTGTGTA 
     1621 CACTTAAACA TGCATTTGAA GCTGGAAACA TGAAAAACCT GGTACTGAAG ATAATCTCCG 
     1681 GATCCTTTCC TCCAGTGTCT CCACATTACT CCTATGATCT CCGCAGCTTG CTGTCTCAGT 
     1741 TATTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGTTTTA 
     1801 TAGCTAAACG AATCGAAAAG TTTCTCTCCC CTCAGCTTAT TGCAGAAGAA TTTTGTCTAA 
     1861 AAACACTTTC AAAGTTTGGA CCACAGCCTC TCCCAGGTAA AAGACCAGCA TCAGGACAAG 
     1921 GTGTCAGTTC TTTTGTCCCT GCTCAGAAAA TCACAAAGCC TGCTGCTAAA TACGGAGTGC 
     1981 CTTTAACATA TAAGAAGTAT GGAGATAAAA AGTTACTTGA GAAAAAACCA CCCCCAAAAC 
     2041 ATAAACAGGC CCATCAAATT CCCGTGAAGA AAATGAATTC TGGAGAAGAA AGGAAGAAAA 
     2101 TGTCTGAGGA AGCAGCAAAA AAAAGAAGGT TGGAATTTAT TGAGAAAGAA AAGAAGCAAA 
     2161 AGGATCAGAT TAGGTTCCTG AAGGCTGAGC AGATGAAGCG GCAAGAGAAG CAGCGGTTGG 
     2221 AGAGGATAAA TAGGGCCAGG GAACAAGGAT GGAGGAATGT TTTAAGGGCT GGTGGAAGCG 
     2281 GTGAAGTAAA GGCTTCCTTT TTTGGCATTG GAGGGGCTGT CTCTCCATCA CCGTGTTCTC 
     2341 CTCGAGGCCA GTATGAACAT TACCATGCCA TTTTTGACCA AATGCAGCGG CTAAGAGCAG 
     2401 AAGATAATGA AGCAAGATGG AAGGGGGGAA TCTATGGTCG ATGGCTCCCA GAAAGGCAAA 
     2461 AAGGACACTT AGCTGTAGAG AGAGCCAACC AAGTGGAAGA ATTCCTACAG CGTAAACGAG 
     2521 AAGCTATGCA GAATAAAGCC CGAGCCGAAG GACACGTGGT TTATTTGGCA AGACTGAGGC 
     2581 AAATAAGACT ACAAAATTTT AATGAGCGCC AACAGATTAA AGCCAAACTT CGTGGTGAGA 
     2641 ATAAAGAAGC TGATGGTACC AAAGGACAAG AAGCAACTGA AGAGACTGAC ATGAGGCTCA 
     2701 AAAAGATGGA GTCACTTAAG GCGCAAACAA ATGCACGTGC TGCTGTACTA AAAGAACAGC 
     2761 TGGAGCGAAA AAGAAAGGAA GCTTATGAAA GAGAAAAGAA AGTATGGGAA GAACATTTGG 
     2821 TGGCGAGGGT AAAAAGCTCA GATGTTCCTC TGCCTTTGGA ACTTCTTGAA ACAGGTGGTT 
     2881 CTCCATCAAA GCAGCAGGTG AAGCCTGTCA TTTCTGTGAC TTCAGCTTTG AAAGAAGTGG 
     2941 GCCTGGATGG AAGTTTAACT GATACCCAGG AAGAAGAAAT GGAAAAGAGT AACAGTGCTA 
     3001 TTTCAAGTAA GCGAGAAATC CTGCGTAGGC TAAATGAAAA TCTTAAAGCT CAAGAGGATG 
     3061 AAAAGGAAAA GCAGCATCAC TCAGGTTCTT GTGAGACCGT TGGTCACAAA GATGAGAGAG 
     3121 AGTATGAGAC AGAAAATGCC ATTTCCTCTG ATCGCAAGAA GTGGGAGATG GGAGGTCAGC 
     3181 TTGTGATTCC TCTCGATGCA GTGACACTGG ATACATCCTT CTCTGCAACC GAAAAACATA 
     3241 CTGTGGGAGA GGTTATTAAA TTAGATTCTA ATGGCTCTCC AAGAAAAGTC TGGGGGAAAA 
     3301 ACCCTACAGA TTCTGTGCTG AAGATACTTG GAGAAGCTGA ATTACAGCTA CAGACAGAAC 
     3361 TACTAGAAAA CACATCTTTT AAAAGTGAGG TTTATGCTGA AGAGGAGAAC TACAAACCCT 
     3421 TACTTACTGA AGAAGAGAAT CTGCAGTGCA TTTCAAAAGA AATAAATCCA TCAGCTACTG 
     3481 TTGATTCTAC TGAAACGAAA AGTCCAAAGT TTACTGAGGT GTCTCCACAA ATGTCAGAAG 
     3541 GAAATGTGGA AGAACCTGAT GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTAGCACAC 
     3601 ACACAGATGG GAGTTTGCCA CCTGTTCTTA ATGATGTGTG GACTAGAGAG AAGGAAGCAG 
     3661 CTAAGGAAAC TGAGTTGGAA GATAAGGTTG CTGTGCAGCA GAGTGAAGTT TGTGAAGATA 
     3721 GAATTCCAGG GAACGTGGAC CAATCCTGTA AGGATCAGAG AGATCCTGCA GTAGACGATT 
     3781 CTCCGCAGTC TGGCTGTGAT GTAGAGAAGT CAGTACAGCC AGAATCGATT TTCCAGAAAG 
     3841 TGGTTCATTC TAAGGACTTG AACTTAGTTC AGGCAGTTCA TTGCTCACCA GAAGAACCAA 
     3901 TTCCAATTCG ATCTCACTCT GATTCTCCAC CAAAAACTAA GAGCAAGAAT TCCTTACTGA 
     3961 TTGGACTTTC AACTGGTCTG TTTGATGCAA ACAATCCAAA GATGCTGAGG ACCTGCTCAC 
     4021 TTCCAGATCT TTCCAAGCTG TTCAGAACCC TAATGGACGT TCCCACTGTG GGGGACGTTC 
     4081 ATCAAGACAG TCTTGAAATC GATGAGCTGG AAGATGAACC AATTAAAGAA GGGCCTTCTG 
     4141 ATTCCGAAGA CACTGTATTT GAAGAAACTG ACACAGATTT ACAAGAGCTT CAGGCCTCAA 
     4201 TGGAGCAGCT GCTTAGGGAG CAACCAGGTG ACGAATACAG TGAGGAGGAA GAGTCTGTTT 
     4261 TAAAAAGCAG CGATGTGGAG CAGACAGCAA GAGGGACAGA TGCCCCAGAC GAGGAGGACA 
     4321 ACCCCAGCAG CGAAAGCGCC CTGAACGAGG AATGGCACTC AGATAATAGT GACGCTGAGA 
     4381 CCACTAGTGA ATGTGAATAT GACAGTGTCT TTAACCATTT AGAGGAACTA AGACTTCACT 
     4441 TGGAGCAAGA AATGGGCTTT GAAAAGTTCT TTGAGGTTTA TGAGAAAGTA AAGGCTATTC 
     4501 ATGAGGATGA AGATGAAAAT ATTGAAATTT GTTCAACAAT AGTTGAGAAT ATTTTGGGCA 
     4561 ATGAGCACCA GCATCTCTAT GCCAAGATTC TGCATTTAGT CATGGCAGAT GGAGCCTATC 
     4621 AGGAAGATAA TGATGAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4681 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4741 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4801 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4861 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4921 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4981 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5041 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5101 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5161 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5221 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5281 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5341 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5401 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6301 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6361 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6421 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6481 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6541 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6601 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6661 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6721 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6781 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6841 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6901 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6961 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7021 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7081 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7141 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7201 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7261 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7321 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7381 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7441 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7501 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7561 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7621 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7681 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7741 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7921 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7981 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8041 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8101 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8161 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8221 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8281 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8341 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8401 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8461 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//