LOCUS       MR216182                8160 bp    DNA     circular  SYN 
DEFINITION  Sema5b (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b)
ACCESSION   NM_013661
VERSION     NM_013661.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4317..4408 
                     /label="Myc-DDK"  
     misc_feature    4438..5023 
                     /label="PolyA signal"  
     misc_feature    4495..4514 
                     /label="Restriction Enzyme"  
     misc_feature    5299..5971 
                     /label="ColE1"  
     misc_feature    6401..7195 
                     /label="Kan/Neo"  
     misc_feature    7230..7532 
                     /label="SV40 ori"  
     misc_feature    7618..8095 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4299..4305 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4299 
                     /label="Sema5b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTAGTCCCA GGACCCCTGG CTCTCTCGCT GTTGCTGTCC AGCCTCACCC 
     1081 TGCTGGTGTC CCACCTCTCC AGCTCCCAGG ACATTGCCAG TGAGTCCAGC AGTGAGCAAC 
     1141 AGATGTGCAC GCGGAGGGAG CACCCCATCG TGGCCTTTGA AGATCTGAAG CCGTGGGTCT 
     1201 TTAACTTCAC CTACCCTGGA GTCCGGGATT TCTCCCAGCT TGCTCTTGAT CCCTCGAGGA 
     1261 ATCAGCTCAT CGTGGGAGCC AGGAACTACC TCTTCAGACT CAGCCTCGCC AATGTCTCCC 
     1321 TCCTTCAGGC TACAGAGTGG GCCTCCAGTG AGGACACGCG CCGCTCCTGC CAGAGCAAAG 
     1381 GGAAAACGGA GGAGGAGTGT CAGAACTATG TACGAGTCCT GATTGTTTCC GGCCGGAAGG 
     1441 TGTTCATGTG CGGTACCAAT GCCTTTTCCC CAGTGTGCTC CAGCAGACAG GTAGGGAACC 
     1501 TCAGCCGGAC TATTGAGAAG ATCAACGGTG TGGCCCGGTG CCCCTATGAC CCACGCCACA 
     1561 ACTCTACAGC CGTCATCTCC TCTCAGGGGG AGCTCTATGC AGCCACAGTC ATTGACTTCT 
     1621 CCGGTCGGGA CCCAGCCATC TACCGCAGCC TGGGCAGTGG GCCACCGCTT CGCACTGCCC 
     1681 AGTATAACTC CAAATGGCTC AATGAGCCAA ATTTTGTGGC AGCCTTTGAC ATCGGGCTGT 
     1741 TTGCGTATTT CTTCCTTCGG GAGAATGCCG TGGAGCATGA TTGTGGGCGC ACTGTGTACT 
     1801 CTCGGGTGGC CCGGGTGTGC AAGAATGATG TAGGTGGCCG TTTCCTGCTG GAGGACACGT 
     1861 GGACCACATT CATGAAGGCC CGGCTCAACT GCTCCCGCCC GGGAGAGGTC CCCTTCTACT 
     1921 ATAATGAGCT GCAGAGTGCC TTCCATCTGC CCGAGCAGGA CCTCATCTAT GGCGTCTTCA 
     1981 CCACTAACGT AAACAGCATT GCGGCTTCTG CTGTCTGCGC CTTCAACCTC AGTGCCATCT 
     2041 CCAAGGCTTT CAATGGCCCA TTTCGTTACC AGGAAAACCC CAGGGCTGCC TGGCTCCCAA 
     2101 TCGCCAATCC CATCCCCAAT TTCCAGTGTG GCACTCTGCC GGAGACTGGC CCCAACGAGA 
     2161 ACCTCACGGA GCGCAGCCTG CAGGACGCAC AGCGGCTCTT CCTGATGAGC GAAGCTGTGC 
     2221 AGCCAGTGAC ACCAGAGCCC TGTGTCACCC AGGACAGCGT CCGCTTCTCA CATCTCGTGG 
     2281 TAGACCTTGT GCAAGCTAAG GACACGCTCT ATCACGTACT CTACATAGGC ACGGAGTCGG 
     2341 GTACCATCCT GAAAGCGCTG TCCACGGCCA GCCGCAGCCT CCGTGGCTGC TACCTGGAGG 
     2401 AGCTACATGT GCTGCCTCCT GGGCGCCTTG AACCTCTGCG GAGCCTGCGC ATCCTGCACA 
     2461 GCGCGCGTGC GCTCTTCGTG GGATTGAGCG ACAGGGTGCT GCGGGTCCCA CTGGAGAGGT 
     2521 GTTCGGCCTA TCATAGCCAG GGGGCATGCC TGGGAGCACG GGACCCATAC TGCGGCTGGG 
     2581 ATGGGAAGCG GCAACTTTGC AGCACGCTTG AAGACAGTTC CAACATGAGC CTGTGGATCC 
     2641 AGAACATCAC AACCTGTCCT GTACGAAATG TGACACGGGA TGGGGGCTTC GGCCCATGGT 
     2701 CACCATGGAA ACCGTGTGAG CACTTAGATG GAGACAACTC GGGTTCTTGC CTGTGCCGGG 
     2761 CCAGATCCTG TGACTCCCCA AGGCCTCGCT GTGGGGGCCT CGAGTGCCTG GGGCCATCCA 
     2821 TCCATATTGC CAATTGCTCC AGGAATGGGG CGTGGACCGC ATGGTCATCG TGGGCTCAGT 
     2881 GCAGCACGTC CTGTGGGATC GGCTTCCAGG TCCGTCAGCG AAGTTGCAGC AACCCGGCGC 
     2941 CCCGCCATGG GGGCCGCATT TGCGTGGGCA AGAGCCGGGA GGAGCGGTTC TGTAATGAAA 
     3001 ATACACCTTG CCCAGTGCCC ATCTTCTGGG CTTCCTGGGG TTCCTGGAGC AAGTGCAGCA 
     3061 ACAACTGTGG AGGCGGCGTG CAGTCGCGAC GCCGTTCTTG CGAGAATGGC AACTCGTGCC 
     3121 CGGGTTGCGG CGTGGAGTTC AAGACCTGCA ACCCCGAGGC TTGCCCGGAA GTGCGACGCA 
     3181 ACACACCCTG GACGCCCTGG CTGCCCGTGA ACGTGACCCA GGGTGGAGCG CGCCAGGAGC 
     3241 AGCGATTCCG CTTCACCTGC CGCGCGCCGC TGCCGGACCC GCACGGTCTG CAGTTCGGCA 
     3301 AGAGGAGGAC AGAGACCAGG ACTTGCCCGG CAGACGGCAC CGGAGCCTGC GACACCGACG 
     3361 CCCTGGTGGA GGATCTCCTG CGCAGCGGGA GCACGTCACC ACACACTCTG AACGGAGGCT 
     3421 GGGCCACCTG GGGCCCGTGG TCATCCTGCT CCCGGGACTG CGAGCTGGGC TTCCGCGTCC 
     3481 GCAAGAGAAC TTGTACCAAC CCGGAGCCTC GCAACGGGGG CTTGCCCTGC GTGGGAGACG 
     3541 CTGCGGAGTA CCAAGACTGC AACCCACAGG CTTGCCCAGT GCGGGGTGCT TGGTCCTGCT 
     3601 GGACCGCATG GTCCCAGTGC TCAGCATCCT GCGGTGGTGG CCACTATCAA CGCACCCGTT 
     3661 CCTGCACCAG CCCCGCCCCA TCCCCAGGTG AGGACATCTG CCTCGGCCTG CACACGGAGG 
     3721 AGGCCCTATG TTCAACACAG GCCTGCCCAG AAGGCTGGTC ACTGTGGTCT GAGTGGGGTG 
     3781 TCTGCACTGA GGATGGGGCC CAGAGCCGGA GCCGGAGCTG TGAGGAGCTT CTCCCAGGAC 
     3841 CAGGTGCCTG TGTTGGCAAC AGCAGCCAGA GCCGGCCCTG CCCCTACAGT GAGATTCCTG 
     3901 TCATCCTACC TGCTTCCAGT GTGGAGGAGA CCACCAGCTG TGGAGGGTTC AATCTCATCC 
     3961 ACCTGATAGT CACTGGTGTG TCCTGCTTCC TGGTTTCTGG GCTCTTGACC TTGGCAGTGT 
     4021 ACCTGTCTTG CCAGCACTGC CAGCGCCAGT CTCAGGAGTC CACGCTTGTC CATCCTGCCA 
     4081 CACCTAACCA CTTGCACTAC AAGGGTGGGG GCACCCCCAA GAATGAGAAG TACACCCCTA 
     4141 TGGAATTCAA GACACTGAAC AAGAACAACT TAATCCCTGA TGACAGAGCC AACTTCTACC 
     4201 CACTGCAGCA GACCAATGTG TATACAACCA CGTACTACCC CAGCCCACTG AACAAGCCCA 
     4261 GCTTCCGGCC TGAGGCCTCA CCTGGACAGC GCTGTTTCCC CAACAGCACG CGTACGCGGC 
     4321 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4381 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4441 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4501 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4561 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4621 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4681 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4741 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4801 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4861 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4921 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4981 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5041 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5101 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5161 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5221 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5281 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5341 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5401 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5461 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5521 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5581 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5641 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5701 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5761 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5821 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5881 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5941 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6001 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6061 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6121 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6181 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6241 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6301 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6361 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6421 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6481 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6541 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6601 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6661 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6721 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6781 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6841 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6901 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6961 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7021 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7081 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7141 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7201 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7261 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7321 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7381 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7441 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7501 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7561 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7621 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7681 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7741 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7801 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7861 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7921 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7981 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8041 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8101 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//